Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Development and Characterization of a New Cell Line from Olive Flounder Paralichthys olivaceus

  • Kim, Ju-Won (Biotechnology Research Division, National Institute of Fisheries Science) ;
  • Oh, Bang Geun (Biotechnology Research Division, National Institute of Fisheries Science) ;
  • Kim, Julan (Biotechnology Research Division, National Institute of Fisheries Science) ;
  • Kim, Dong-Gyun (Biotechnology Research Division, National Institute of Fisheries Science) ;
  • Nam, Bo-Hye (Biotechnology Research Division, National Institute of Fisheries Science) ;
  • Kim, Young-Ok (Biotechnology Research Division, National Institute of Fisheries Science) ;
  • Park, Jung Youn (Biotechnology Research Division, National Institute of Fisheries Science) ;
  • Cheong, JaeHun (Dept. of Integrated Biological Science, Pusan National University) ;
  • Kong, Hee Jeong (Biotechnology Research Division, National Institute of Fisheries Science)
  • Received : 2018.07.12
  • Accepted : 2018.08.31
  • Published : 2018.09.30
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Abstract

A new embryonic cell line (OFEC-17FEN) derived from olive flounder Paralichthys olivaceus was developed. OFEC-17FEN cells were subcultured for <30 passages over ~200 days. OFEC-17FEN cells had a doubling time of 114.34 h and modal diploid chromosome number was 48. The pluripotency genes POU5f1 and NANOG were expressed in OFEC-17FEN cells. However, the lack of several pluripotency-related genes expression indicates that OFEC-17FEN cells are not stem cells. OFEC-17FEN cells transfected with plasmid pEGFP-c1 exhibited a strong green fluorescent signal at 48 h after transfection. Accordingly, OFEC-17FEN cells may be useful for both basic research and biotechnological application.

Keywords

References

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