Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Silk Protein as a Fetal Bovine Serum Substitute for Animal Cell Culture

  • Jo, You-Young (Sericultural and Apicultural Materials Division, National Institute of Agricultural Science, RDA) ;
  • Kweon, HaeYong (Sericultural and Apicultural Materials Division, National Institute of Agricultural Science, RDA) ;
  • Ji, Sang Deok (Sericultural and Apicultural Materials Division, National Institute of Agricultural Science, RDA) ;
  • Kim, Jong Gil (Sericultural and Apicultural Materials Division, National Institute of Agricultural Science, RDA) ;
  • Kim, Kee Young (Sericultural and Apicultural Materials Division, National Institute of Agricultural Science, RDA)
  • Received : 2019.01.30
  • Accepted : 2019.03.21
  • Published : 2019.12.28
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Abstract

Fetal Bovine Serum (FBS) is an essential substance added to animal cell culture medium. However, its composition is unclear causing problems such as development of an immune response when cultured cells are transplanted into the human body. In this study, silk sericin, silk fibroin, and hemolymph obtained from silkworms were added to the cell culture medium in order to determine if it can replace FBS. After establishment of the cell culture, cell proliferation and expression levels of cell growth-related genes were compared with those of control cells (cells cultured in the medium with 10% FBS). Results showed that the test group treated with silk fibroin extracted from a Korean silkworm variety, Kumokjam could replace 10% FBS. In addition, expression levels of cell growth related genes such as Fibronectin and TGF-β1 increased significantly in cells cultured using silk fibroin, depending on the concentration used in cell adhesion and cell proliferation [24]. To date, no studies have been conducted to find a replacement for FBS. Thus, this study was carried out to develop a substitute for FBS by using silkworm-derived alternatives such as silkworm hemolymph, silk sericin, and silk fibroin, which are cheap and have various physiological effects, cell promoting effects, and can be mass produced.

Keywords

References

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