• 동의생리병리학회지
• /
• 제28권3호
• /
• pp.330-336
• /
• 2014

This study aimed at examining the anti-inflammatory effects of Scutellariae Radix & Lonicerae Caulis water extract(SC). RAW 264.7 mouse macrophage cells were treated with $25{\sim}200{\mu}g/m{\ell}$ SC for 24 hours. Cell viability was then measured using MTT assays. The nitric oxide(NO) production and the creation of several cytokines in LPS-stimulated RAW 264.7 cells were investigated. SC inhibited significantly increasing the production of NO in LPS-induced RAW 264.7 cell at the density of 25, 50 and $200{\mu}g/m{\ell}$. SC inhibited significantly the TNF-${\alpha}$ of the RAW 264.7 cell induced by LPS at the density of $50{\mu}g/m{\ell}$. SC inhibited significantly the MIP-$1{\alpha}$ of the RAW 264.7 cell induced by LPS at the density of 25, 50 and $100{\mu}g/m{\ell}$. SC inhibited significantly the MIP-$1{\beta}$, MIP-2 at the density of 50, $100{\mu}g/m{\ell}$ in the RAW 264.7 cell increased by LPS, respectively. SC did not affect the production levels of VEGF in RAW 264.7 cell. As a result, SC significantly inhibited the inductions of MIP-$1{\alpha}$, MIP-$1{\beta}$, MIP-2 and NO in LPS-induced RAW 264.7 cell without causing the toxicity. These results signify that SC has anti-inflammatory effects on controlling the over inflammatory reaction on the RAW 264.7 cell.

• ALGAE
• /
• 제31권4호
• /
• pp.391-401
• /
• 2016

To develop an easy and rapid method of quantifying lipid contents of marine dinoflagellates, we quantified lipid contents of common dinoflagellate species using a colorimetric method based on the sulpho-phospho-vanillin reaction. In this method, the optical density measured using a spectrophotometer was significantly positively correlated with the known lipid content of a standard oil (Canola oil). When using this method, the lipid content of each of the dinoflagellates Alexandrium minutum, Prorocentrum micans, P. minimum, and Lingulodinium polyedrum was also significantly positively correlated with the optical density and equivalent intensity of color. Thus, when comparing the color intensity or the optical density of a sample of a microalgal species with known color intensities or optical density, the lipid content of the target species could be rapidly quantified. Furthermore, the results of the sensitivity tests showed that only $1-3{\times}10^5cells$ of P. minimum and A. minutum, $10^4cells$ of P. micans, and $10^3cells$ of L. polyedrum (approximately 1-5 mL of dense cultures) were needed to determine the lipid content per cell. When the lipid content per cell of 9 dinoflagellates, a diatom, and a chlorophyte was analyzed using this method, the lipid content per cell of these microalgae, with the exception of the diatom, were significantly positively correlated with cell size, however, volume specific lipid content per cell was negatively correlated with cell size. Thus, this sulpho-phospho-vanillin method is an easy and rapid method of quantifying the lipid content of autotrophic, mixotrophic, and heterotrophic dinoflagellate species.

• Journal of Microbiology and Biotechnology
• /
• 제6권3호
• /
• pp.221-224
• /
• 1996

Pseudomonas putida BM01 was grown efficiently on glucose as the sole carbon source with a supply of a nitrogen source in pH-stat mode using a low setpoint limit. A final cell concentration of 100 g/l was obtained in 30 h of fed-batch cultivation by controlling glucose concentration within the range of 5-20 g/l and maintaining dissolved oxygen tension above 10$%$ saturation using pure oxygen. This high cell density culture technique is believed highly useful for the production of poly(3-hydroxyalkanoates) by this strain.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2001년도 추계학술발표대회
• /
• pp.845-848
• /
• 2001

For understanding physiology and metabolism under various culture conditions, combined analysis of transcriptome and proteome is attractable way. We have manufactured DNA microarray containing 2,850 genes including all functionally known and putative ones. In this study, we report analysis of transcriptome and proteome during the high cell density culture of E. coli by using DNA microarray and 2-DE. Fed-batch fermentation of E. coli was carried out by exponential feeding of nutrients until the maximum cell density reached 74 g dry cell weight/L (g DCW/L). Changes in transcriptome and proteome during the HCDC are analyzed qualitatively and quantitatively to provide their physiological and metabolic meanings.

• 한국미생물·생명공학회지
• /
• 제7권1호
• /
• pp.47-49
• /
• 1979

Undigested cellulosic substrate was frequently replaced with new substrate during fermentation. About 3-fold increase in cell density could be obtained by changing the substrate.

• 한국표면공학회지
• /
• 제37권2호
• /
• pp.92-98
• /
• 2004

The current efficiency and the microstructure of the trivalent Cr deposits plated in flow cell system were investigated according to additives in sulfate bath and current density. The current efficiency of the deposits plated in the formic acid complexed bath was noticeably higher than that of the deposits from glycine complexed bath. The current efficiency of the deposits from the complexed baths with boric acid buffer increased linearly with current density in the range of 60-100 A/dm$^2$, while that of the deposits from the baths with both Al sulfate and mixed buffers increased parabolically with current density. The nodular crystallite size of the deposits increased with current density, and the deposits plated in low current density region had relatively smooth surface appearance with fine grains. The structure of the deposits from the complexed baths with boric acid buffer changed from amorphous structure to crystalline one with strong (110)peak with increasing current density. The deposits from the baths with both Al sulfate and mixed buffers had generally amorphous structure.

• 한국자기학회:학술대회 개요집
• /
• 한국자기학회 2015년도 임시총회 및 하계학술연구발표회
• /
• pp.78-79
• /
• 2015

We investigate the switching current for various cell diameters and DM interaction. We find that the current density for switching can depend strongly on the cell size when the switching is governed by the domain wall motion. Moreover the switching current density is also strongly influenced by DM interaction. In the presentation, we will discuss the effect of domain wall formation and more various DMI constant on the switching current desity in detail.

• 대한기계학회논문집B
• /
• 제27권5호
• /
• pp.635-643
• /
• 2003

Parametric study for the analysis of performance characteristics of a planar -type solid oxide fuel cell(SOFC) using computational flow analysis is conducted. A planar -type SOFC, which is composed by two gas channels (fuel and ai.) and one set of anode-electrolyte-cathode assembly, is modeled as a two -dimensional isothermal case. Results of computational analysis of flow field including distributions of mass fractions in gas channels are used to the performance analysis of the fuel cell. Flow analysis makes it possible to consider current density distributions along the length of the cell in the process of performance analysis of the SOFC. As results of parametric study, it is found that the mole fraction of fuel at the inlet of fuel channel, operating pressure and temperature are closely related to the performance characteristics of SOFC.

• Composites Research
• /
• 제17권2호
• /
• pp.34-39
• /
• 2004

본 연구에서는 폐쇄형 발포금속의 인장 특성을 이해하기 위하여 수정된 단위모델을 제시하였다. 또한 발포금속의 밀도는 가우스 분포에 의거하여 확률적으로 분포한다고 가정하고 본 연구에서 제시된 수정 단위 모델을 조합하여 유한요소 모델을 제안하였다. 이 모델은 실제 인장 시험과 유사한 변형거동을 보이는 것을 확인하였고, 적절한 밀도 분포와 내부 기공을 고려하게 되면, 해석에서 구해진 최대 인장 강도가 근사적으로 실험결과와 일치하는 것을 볼 수 있었다. 또한, 발포 알루미늄의 최대 인장 강도는 밀도 분포의 표준편차보다는 내부 기공 부피분율에 더 민감하게 변하는 것으로 밝혀졌다.

• 한국환경과학회지
• /
• 제23권10호
• /
• pp.1745-1753
• /
• 2014

Sediment microbial fuel cell (SMFC), equipped with Zn, Al, Cu, Fe or graphite felt (GF) anode and marine sediment, was performed. Graphite felt was used as a common cathode. SMFC was single chambered and did not use any redox mediator. The aim of this work was to find efficient anodic material. Oxidation reduction potential (ORP), cell voltage, current density, power density, pH and chemical oxygen demand (COD) were measured for SMFC's performance.. The order of maximum power density was $913mWm^{-2}$ for Zn, $646mWm^{-2}$ for Fe, $387.8mWm^{-2}$ for Cu, $266mWm^{-2}$ for Al, and $127mWm^{-2}$ for graphite felt (GF). The current density over voltage was found to be strongly correlated with metal electrodes, but the graphite felt electrode, in which relatively weaker electricity was observed because of its bio-oriented mechanism. Metal corrosion reactions and/or a complicated microbial electron transfer mechanism acting around the anodic compartment may facilitate to generate electricity. We presume that more sophisticated selection of anodic material can lead to better performance in SMFC.