• Title/Summary/Keyword: Cell density

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Development of Inexpensive High Energetic Electrodes Ni-Cu and Ni-CeO2-Cu for Renewable Energy through Direct Ethanol Fuel Cell

  • Guchhait, Sujit Kumar;Paul, Subir
    • Journal of Electrochemical Science and Technology
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    • v.7 no.3
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    • pp.190-198
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    • 2016
  • Application of fuel cell to produce renewable energy for commercial purpose is limited by the high cost of Pt based electrode materials. Development of inexpensive, high energetic electrode is the need of the hour to produce pollution free energy using bio-fuel through a fuel cell. Ni-Cu and Ni-CeO2-Cu electrode materials, electro synthesized by pulse current have been developed. The surface morphology of the electrode materials is controlled by different deposition parameters in order to produce a high current from the electro-oxidation of the fuel, the ethanol. The developed materials are electrochemically characterized by Cyclic Voltammetry (CV), Chronoamperometry (CA) and Potentiodynamic polarization tests. The results confirm that the high current is due to their enhanced catalytic properties viz. high exchange current density (i0), low polarization resistance (Rp) and low impedance. It is worthwhile to mention here that the addition of CeO2 to Ni-Cu has outperformed Pt as far as the high electro catalytic properties are concerned; the exchange current density is about eight times higher than the same on Pt surface. The morphology of the electrode surface examined by SEM and FESEM exhibits that the grains are narrow and sub spherical with 3D surface, containing vacancies in between the elongated grains. The fact has enhanced more surface area for electro oxidation of the fuel, giving rise to an increase in current. Presence of Ni, CeO2, and Cu is confirmed by the XRD and EDXS. Fuel cell fabricated with Ni-CeO2-Cu material electrode is expected to produce clean electrical energy at cheaper rates than conventional one, using bio fuel the derived from biomass.

Survey of Corneal Thickness, Endothelial Cell and Intraocular Pressure in New Zealand White Rabbits (뉴질랜드 흰 토끼의 각막 두께, 내피세포 및 안압에 관한 조사)

  • Chang, Hwa-Seok;Lee, Dae-Yeoup;Kang, Eun-Hee;Lee, Jae-Hoon;Cho, Yang-Kyeung;Kim, Man-Soo;Kim, Hwi-Yool
    • Journal of Veterinary Clinics
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    • v.27 no.4
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    • pp.411-414
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    • 2010
  • Rabbit's cornea is the most common thing that used for researches in ophthalmology, so many experimentations have done. The purpose of this study was to survey the corneal pachymetry, tonometry and specular microscopy in New Zealand white rabbits. Three months old male rabbits ($2.9{\pm}0.2\;kg$) were examined with contact the specular microscope to evaluate the endothelial cell density, coefficiency of variation and the percentage of hexagonal cells in the central cornea. For the pachymetry, ultrasonic pachymeter was used for central cornea in each twenty rabbits, and tonometry was performed forty rabbit's eyes to evaluate the intraocular pressure. Corneal endothelial cell density in New Zealand white rabbit was $3304{\pm}354\;cell/mm^2$. Coefficiency of variation was $45.8{\pm}2$ and the percentage of hexagonal cells in the central cornea was $44.4{\pm}11%$. The pachymetry of cornea was $346.1{\pm}21\;{\mu}m$ and tonometry of eye was $14.3{\pm}2\;mmHg$. It is considered that normal New Zealand white rabbit's pachymetry, specular microscopy and tonometry were useful basic data of rabbit's cornea for research and clinic in veterinary ophthalmology.

Enhancing Electricity Generation Using a Laccase-Based Microbial Fuel Cell with Yeast Galactomyces reessii on the Cathode

  • Chaijak, Pimprapa;Sukkasem, Chontisa;Lertworapreecha, Monthon;Boonsawang, Piyarat;Wijasika, Sutthida;Sato, Chikashi
    • Journal of Microbiology and Biotechnology
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    • v.28 no.8
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    • pp.1360-1366
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    • 2018
  • The fungi associated with termites secrete enzymes such as laccase (multi-copper oxidase) that can degrade extracellular wood matrix. Laccase uses molecular oxygen as an electron acceptor to catalyze the degradation of organic compounds. Owing to its ability to transfer electrons from the cathodic electrode to molecular oxygen, laccase has the potential to be a biocatalyst on the surface of the cathodic electrode of a microbial fuel cell (MFC). In this study, a two-chamber MFC using the laccase-producing fungus Galactomyces reessii was investigated. The fungus cultured on coconut coir was placed in the cathode chamber, while an anaerobic microbial community was maintained in the anode chamber fed by industrial rubber wastewater and supplemented by sulfate and a pH buffer. The laccase-based biocathode MFC (lbMFC) produced the maximum open circuit voltage of 250 mV, output voltage of 145 mV (with a $1,000{\Omega}$ resistor), power density of $59mW/m^2$, and current density of $278mA/m^2$, and a 70% increase in half-cell potential. This study demonstrated the capability of laccase-producing yeast Galactomyces reessii as a biocatalyst on the cathode of the two-chamber lbMFC.

Effect of Serum Type on Hybridoma Growth and Monoclonal Antibody Production (하이브리도마 세포증식과 단일클론항체 생산에 미치는 혈청 종류의 영향)

  • 전복환;박송용
    • KSBB Journal
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    • v.9 no.3
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    • pp.253-265
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    • 1994
  • We have studied the effects of serum concentration and initial cell density on hybridoma cell growth and monoclonal antibody (MAb) production at various media supplemented with different types of serum. The types of serum were fetal bovine sera, newborn bovine calf sera, calf sera including supplemented calf sera, horse serum, and goat serum. The concentrations of each serum were 0.5, 1.25, 2.5, and 5% (v/v) and the inoculum densities were $5{\times}10^4, 1{\times}10^5, 2{\times}10^5,$ cells/ml. The hybridoma cell growth and anti-Hepatitis B surface antigen (anti-HBsAg) MAb production were found to be enhanced by increasing the serum concentration and by increasing inoculum density regardless of serum type. We found that test sera purchased from different companies show different effects on cell growth and MAb production, although they are the same type of serum.

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A Novel Atomic Layer Deposited Al2O3 Film with Diluted NH4OH for High-Efficient c-Si Solar Cell

  • Oh, Sung-Kwen;Shin, Hong-Sik;Jeong, Kwang-Seok;Li, Meng;Lee, Horyeong;Han, Kyumin;Lee, Yongwoo;Lee, Ga-Won;Lee, Hi-Deok
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.14 no.1
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    • pp.40-47
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    • 2014
  • In this paper, $Al_2O_3$ film deposited by thermal atomic layer deposition (ALD) with diluted $NH_4OH$ instead of $H_2O$ was suggested for passivation layer and anti-reflection (AR) coating of the p-type crystalline Si (c-Si) solar cell application. It was confirmed that the deposition rate and refractive index of $Al_2O_3$ film was proportional to the $NH_4OH$ concentration. $Al_2O_3$ film deposited with 5 % $NH_4OH$ has the greatest negative fixed oxide charge density ($Q_f$), which can be explained by aluminum vacancies ($V_{Al}$) or oxygen interstitials ($O_i$) under O-rich condition. $Al_2O_3$ film deposited with $NH_4OH$ 5 % condition also shows lower interface trap density ($D_{it}$) distribution than those of other conditions. At $NH_4OH$ 5 % condition, moreover, $Al_2O_3$ film shows the highest excess carrier lifetime (${\tau}_{PCD}$) and the lowest surface recombination velocity ($S_{eff}$), which are linked with its passivation properties. The proposed $Al_2O_3$ film deposited with diluted $NH_4OH$ is very promising for passivation layer and AR coating of the p-type c-Si solar cell.

Effects of Concentrations of Glucose and Maltose on the Growth of Bacillus amyloliquefaciens (B. amyloliquefaciens 세포 성장에 미치는 포도당과 맥아당 농도의 영향에 관한 연구)

  • 차월석;박승규김종수
    • KSBB Journal
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    • v.9 no.4
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    • pp.428-435
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    • 1994
  • Cell growth and production of ${\alpha}$-amylase, acetic acid and lactic acid were investigated in Bacillus amyloliquefaciens(ATCC 23350) flask culture with various carbon sources. Maximum dry cell density increased with increase in initial maltose concentration. Maximum dry cell density was the highest(1.4g/$\ell$) at 10g/$\ell$ of initial glucose concentration. With 10g/$\ell$ of initial glucose concentration, maximum specific cell growth rate was obtained. Among the various carbon sources maximum ${\alpha}$-amylase production was obtained with 149 unit/ml at 20g/$\ell$ of initial maltose concentration. With 5g/$\ell$ of initial maltose concentration, maximum ${\alpha}$-amylase production rate was obtained. By increasing carbon source concentration, acetic acid formation decreased. Acetic acid formation was higher in glucose than in maltose. By increasing carbon source concentration, lactic acid formation increased. Lactic acid formation was higher in maltose than in glucose.

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Dynamic Inter-Cell Interference Avoidance in Self-Organizing Femtocell Networks (자가구성 펨토셀의 동적 셀간간섭 회피 기법)

  • Park, Sang-Kyu;Bahk, Sae-Woong
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.36 no.3A
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    • pp.259-266
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    • 2011
  • Femtocells are expected as the surest way to increase the system capacity with higher-quality links and more spatial reuse in future networks. In spite of their great potential, the system capacity is highly susceptible to network density because a large portion of users are exposed to inter-cell interference (ICI). In this work, we proposed a dynamic interference avoidance scheme in densely deployed cell environments. Our proposed DDIA (Distributed Dynamic ICI Avoidance) scheme not only works in a fully distributed manner, but also controls interference link connectivity of users with high agility so that it is suited for self-organizing networks (SONs). We introduced the concept of ICI-link and two-tier scheduling in designing the DDIA scheme. To avoid ICI without any central entity, our scheme tries to harmonize all base stations (BSs) with users adaptively. Through extensive simulations, it was shown that our proposed scheme improves the throughput of users by more than twice on average compared to the frequency reuse factor 1 scheme, who are exposed to ICI while maintaining or even improving overall network performance. Our scheme operates well regardless of network density and topology.

Fine Structure of the Integumentary Supporting Cell and Gland Cell of the Sea Bass, Lateolabrax japonicus (Teleostei: Moronidae) (농어 (Lateolabrax japonicus) 피부계 지지세포와 선세포의 미세구조)

  • Lee, Jung-Sick;Kim, Jae-Won
    • Applied Microscopy
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    • v.29 no.4
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    • pp.417-426
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    • 1999
  • The present investigation is designed to provide basic information on fine structure of the skin of the sea bass, Lateolabrax japonicks in relation to study of epidermal change with environmental and physiological change. The skin of the sea bass is divided into the epidermal layer and dermal layer. Epidermal layer consists of supporting cells and unicellular glands. The supporting cells were classified into the superficial cell, intermediated cell and basal cell. Gland cells were classified into the mucous secretory cell and club cell which is more frequently observed. Superficial cell of epidermal layer is squamous or cuboidal and contains well-developed rough endoplasmic reticulum and the surface is covered with numerous microridges. Superficial cells are connected to another cell with membrane interdigitations and desmosomes. Intermediated cell is ovoid and the electron density is higher than the other supporting cells. Basal cell is cuboidal and has a well-developed mitochondria and membrane interdigitation. The mucous secretory cell has a numerous membrane bounded secretory granules. The cytoplasm of club cell is divided into cortex and medullar. The medullar cytoplasm has a nucleus, intracellular organelles and central vacuole, and the cortical cytoplasm has a well-developed tonofilament. Club cells are connected to another cell with well -developed membrane interdigitations and desmosomes.

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Development of a predictive model of the limiting current density of an electrodialysis process using response surface methodology

  • Ali, Mourad Ben Sik;Hamrouni, Bechir
    • Membrane and Water Treatment
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    • v.7 no.2
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    • pp.127-141
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    • 2016
  • Electrodialysis (ED) is known to be a useful membrane process for desalination, concentration, separation, and purification in many fields. In this process, it is desirable to work at high current density in order to achieve fast desalination with the lowest possible effective membrane area. In practice, however, operating currents are restricted by the occurrence of concentration polarization phenomena. Many studies showed the occurrence of a limiting current density (LCD). The limiting current density in the electrodialysis process is an important parameter which determines the electrical resistance and the current utilization. Therefore, its reliable determination is required for designing an efficient electrodialysis plant. The purpose of this study is the development of a predictive model of the limiting current density in an electrodialysis process using response surface methodology (RSM). A two-factor central composite design (CCD) of RSM was used to analyze the effect of operation conditions (the initial salt concentration (C) and the linear flow velocity of solution to be treated (u)) on the limiting current density and to establish a regression model. All experiments were carried out on synthetic brackish water solutions using a laboratory scale electrodialysis cell. The limiting current density for each experiment was determined using the Cowan-Brown method. A suitable regression model for predicting LCD within the ranges of variables used was developed based on experimental results. The proposed mathematical quadratic model was simple. Its quality was evaluated by regression analysis and by the Analysis Of Variance, popularly known as the ANOVA.

Change of Blooming Pattern and Population Dynamics of Phytoplankton in Masan Bay, Korea (마산만 식물플랑크톤의 대발생 양상의 변화와 군집 동태)

  • Lee, Ju-Yun;Han, Myung-Soo
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.12 no.3
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    • pp.147-158
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    • 2007
  • To clarify the bloom pattern and species succession in phytoplankton community, the population dynamics with the determination of physico-chemical factors have been studies in Masan Bay, the south sea of Korea, for the periods November 2003-October 2004. Concentration of $NH_4-N$ was always higher than that of $NO_3-N$, which was similar level as compared to other costal areas. $PO_4-P$ concentration was lower than those in other coastal areas but similar to oligotrophic environments. Thus, phosphate seems the limiting nutrient rather than nitrogen. $SiO_2-Si$ concentration was also low as compared to other costal areas. Si:P ratio was low from autumn to winter, suggesting silicate and/or phosphate limitation during this period. The cell density of phytoplankton was high in winter 2003 and early autumn 2004. The carbon biomass was high in winter 2003 and summer 2004. And chlorophyll-a concentration was high in late autumn 2003 and summer 2004. Among 78 species of phytoplankton found in the bay during the investigated period, dominant species were two diatoms of Cylindrotheca closterium, Skeletonema costatum, and three dinoflagellates of Heterocapsa triquetra, Prorocentrum minimum, P. triestinum, and one raphidophyte of Heterosigma akashiwo. P. minimum dominated from late autumn to winter, but it was replaced by H. triquetra in late winter. P. triestinum dominated from late spring to early summer. Simultaneously, H. akashiwo cell density steadily increased, and it became dominant with C. closterium in late summer. With decreasing of H. akashiwo and C. closterium, S. costatum became the most dominant species in autumn. The canonical analyses showed that total phytoplankton cell density related to diatom cell density and it was affected by temperature, and concentrations of $NO_3-N\;and\;PO_4-P$. The carbon bio-mass and $chlorophyll-{\alpha}$ concentration related to diatom- and dinoflagellate cell densities and these were affected by flagellate cell density, salinity, and concentrations of $SiO_2-Si\;and\;PO_4-P$. Last six years monitoring data in Masan city obtained from Korean Meteorological Agency indicates gradual increase in air temperature. And the precipitation decreased especially in spring season. The winter bloom found in 2003 may be caused by the increase in the temperature and this bloom subsequently induced the nutrients depletion, which continued until next spring probably due to no precipitation. Therefore, the spring bloom, which had been usually observed in the bay, might disappear in 2004.