• Title/Summary/Keyword: Cell density

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Lipids Supply for Hybridoma Culture (하이브리도마 배양을 위한 lipids의 공급)

  • 박상재;최차용
    • KSBB Journal
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    • v.8 no.5
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    • pp.483-487
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    • 1993
  • BSA/acids component in serum free medium (SFM) developed for the culture of hybridoma cell line, KA112, was replaced by acids/Pluronic F-68 emulsion. Protein content of SFM was minimized, and increased maximum cell density was obtained in serum-free lipids supplemented medium (SFLSM). Cell growth promotion effect of the emulsion was not affected by filtration with 0.2$\mu$m filter.

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A Study on Heat Transport Phenomena in Fuel Cell Stack for the Performance Analysis of Phosphoric Acid Fuel Cell (인산형 연료전지의 성능해석을 위한 스택내의 열전달 현상에 관한 연구)

  • Moon, Duk-Yong;Gu, Ja-Yong;Seo, Jong-Chul;Kim, Yu
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 1991.11a
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    • pp.160-164
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    • 1991
  • The effect of various parameters, such as temperature, current density and operating valtage on the performance of phosphoric acid fuel cell stack was studied by using numerical analysis. The utilization ratio of reaction gas, inlet condition of reaction air and cooling air, inlet condition of cooling air flow latin were changed regularly, The results showed good agreements with the existing results and experimental ones.

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Continuous high cell density culture of Anaerobiospirillum succiniciproducens with membrane filtration for the production of succinic acid

  • Lee, Pyeong-Cheon;Lee, U-Gi;Lee, Sang-Yeop;Jang, Ho-Nam
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.338-341
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    • 2000
  • An internal membrane bioreactor system was employed for continuous succinic ac id production from glucose in order to prove its performance and practicality. Succinic acid-producing Anaerobiospirillum succiniciproducens required more $CO_2$ for the proper growth and succinic acid production in cell recycled continuous culture than in batch culture. The maximum productivity obtained in cell recycled continuous culture was about 3.3 g/L-h which was ca. 3.3 times higher than that obtained in batch culture.

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Experimental Investigation of the Excited Xe Atoms Density in Ultra-high-resolution PDPs

  • Ishii, Keiji;Hirano, Yoshikuni;Murakami, Yukio
    • 한국정보디스플레이학회:학술대회논문집
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    • 2008.10a
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    • pp.61-64
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    • 2008
  • We investigated experimentally the influence of the generation efficiency for excited Xe atoms on ultra-fine horizontal cell pitch AC PDPs. In the case of a 0.1 mm cell pitch, the sustain voltage increased; however, the generation efficiency was equal to or higher than for the conventional 0.22 mm cell pitch.

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Monte Carlo Simulation on the Adsorption Properties of Methane in Zeolite L

  • 문성두;Yoshimori Miyano
    • Bulletin of the Korean Chemical Society
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    • v.18 no.3
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    • pp.291-295
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    • 1997
  • The adsorption of methane in K+ ion exchanged zeolite L has been studied using grand canonical ensemble Monte Carlo simulation. Average number of molecules per unit cell, number density of molecules in zeolite, distribution of molecules per unit cell, average potential per sorbate molecule, and isosteric heats of adsorption were calculated, and these results were compared with experimental results. The simulation results agreed fairly well with experimental ones. All methane molecules were located in the main channel, and the average potential of sorbate molecule was almost constant regardless of average number of molecules per unit cell and the amounts sorbed in zeolite.

Performance Analysis of PEMFC Using Computational Flow Dynamics (CFD) (전산유체역학 (CFD)을 이용한 PEMFC의 성능분석)

  • Kim, Sunhoe
    • Journal of Digital Convergence
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    • v.11 no.8
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    • pp.199-204
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    • 2013
  • Computational fluid dynamics (CFD) is used to reduce number of experiments. The CFD tools are widely used for engine design and flow pattern analysis to reduce experiments. In this study the performance of a PEMFC single cell was analyzed by using STAR-CD, product of CD-ADAPCO. The effect of cell design and flow pattern on the performance of a PEMFC was analyzed with the 3-D simulation. As a result the performance of rectangular cell was the higher than that of square cell, while the flow direction scarcely affected on the performance of a PEMFC. Also the current density according to different excess ratio of air flow rate was compared and analyzed. The difference between maximum and minimum current density of flip-flow was lower than that of co-flow.

Characteristics of Culm Anatomy and Dimensional Variation in Genus Phyllostachys Grown Damyang District, Korea (담양지방(潭陽地方) 왕대속(屬) 4종(種)의 조직(組織) 및 간내(稈內) 변이성(變異性))

  • Lee, Jae-Ki;Park, Sang-Jin
    • Journal of the Korean Wood Science and Technology
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    • v.15 no.3
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    • pp.14-23
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    • 1987
  • This study was carried out to investigate the anatomical characteristics, the radial and axial variation of the cell dimension and bulk density for four bamboo species used as the major material of bamboo products in Korea; Phyllostachys(P.bambusoides, P.edulis, P.nigra var.henonis, and Pnigra). The results obtained were summarized as follows; 1) In the outer part of culm, the mophological difference of cell diameter and distribution were not acknowledged. However, gum-like substance was occurred In the epidermis, hypodermis, cortex and metaxylem vessel of P.nigra, while it was occured only in the metaxylem vessel of P.edulis. 2) The protoxylem vessel in the central zone of culm-wall could be in the two types; the first type consisted of 4-5 small cell pieces occurred in P.edulis and P.nigra var. henonis, the second type of 1-2 large cell pieces in P.bambusoides and P.nigra. And transition of cell diameter from the vascular bundle sheath to the ground tissue was gradually enlarged in P.nigra and P.edulis but abruptly in P.bambusoides and P.nigra var. henonis. 3) Thin-wall tyloses were well developed in the outer and central zone of culm-wall in four species. 4) Compared with the central part of culm-wall, the number of parenchyma cells and the amount of the vascular bundle sheaths per unit area were a few in the inner part. The inner part nearest to the pith cavity was consisted of thick-well parenchyma cell. 5) The dimensional variations of metaxylem vessel and parenchyma cell in the radial direction were decreased from the inner part to the epidermis in all species observed. 6) The fiber length was the maximum in the central zone and its diameter was increased from the inner part to the outer part. In the axial variation. fiber length was slightly increased from the base and then decresed slowly toward the top, and its diameter was reverse. 7) The axial variation of the bulk density was continuously increased from the base toward the top and its radial variation was rapidly increased from the inner part to the epidermis.

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Planar fuel cell design integrated with methanol reformer by using a high temperature membrane (고온형 멤브레인을 사용한 메탄올 개질 연료전지의 개질기 일체형 평판 설계)

  • Kim, Sung-Han;Jang, Jae-Hyuk;Gil, Jae-Hyoung;Lee, Hong-Ryul;Cha, Hye-Yeon;Ku, Bo-Sung;Jung, Chang-Ryul;Kundu, Arunaha;Miesse, Craig;Oh, Yong-Soo
    • 한국신재생에너지학회:학술대회논문집
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    • 2006.11a
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    • pp.467-470
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    • 2006
  • For a mobile application such as cellular phone, micro fuel cells should be extremely compact and thin. RHFC can be an alternative solution because RHFC gives higher power density than DMFC and does not need ahydrogen storage vessel In this paper, RHFC using methanol fuel is made as a novel planar design without a PROX. Both reformer and cell are made closely in a same plate to share the heater of reformer with the cell. The PBI membrane is used in the cell. The reason is that high temperature of reformer can cause a performance drop when perfluorosulfonic acid membrane such as Nafion is used such a high temperature operation also guarantees the higher CO tolerance to MEA catalyst. The cell is designed as an air-breathing type which the cathode of the cell is opened to the air. The commercial Cu/ZnO/Al2O3 steam reformer catalyst is packed in reformer channel. The active area of MEA is $11.9cm^2$ and the peak power density was $27.5mW/cm^2$.

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Performance Characteristics of Li-ion Battery and Supercapacitor Hybrid Cell for High Power / Low Temperature Discharge (고출력/저온 방전을 위한 리튬전지와 슈퍼캐패시터 하이브리드 셀의 방전 거동 특성 연구)

  • Jang, Woojin;Hong, Seung-Chul;Hong, Jung-Pyo;Hwang, Taeseon;Oh, Joon-Suk;Ko, Sungyeon;Lee, Gaeun;Ahn, Kyunyoung;Kim, Hyunsoo;Suhr, Jonghwan;Nam, Jae-Do
    • Transactions of the Korean Society of Automotive Engineers
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    • v.21 no.6
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    • pp.49-57
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    • 2013
  • In this study, we fabricated a parallelly connected Li-ion battery/supercapacitor hybrid cell to combine the advantageous characteristics of Li-ion battery and supercapacitor, high energy density and high power density, respectively, and investigated its discharging characteristics over a wide temperature range from -40 to $25^{\circ}C$. At the initial state of discharging of the hybrid cell, the power was mostly provided by the supercapacitor and then the portion of the Li-ion battery was gradually increased. By installing a switching system into the hybrid cell, which controls the discharging sequence of Li-ion battery and supercapacitor, the maximum power was improved by 40% compared with non switching system. In addition at low temperatures, the power and discharging time of the hybrid cell were significantly enhanced compared to a battery-alone system. The hybrid cell is expected to be applied in electric vehicles and small domestic appliances that require high power at initial discharging state.

Can a Fermentation Gas Mainly Produced by Rumen Isotrichidae Ciliates be a Potential Source of Biohydrogen and a Fuel for a Chemical Fuel Cell?

  • Piela, Piotr;Michalowski, Tadeusz;Miltko, Renata;Szewczyk, Krzysztof W.;Sikora, Radoslaw;Grzesiuk, Elzbieta;Sikora, Anna
    • Journal of Microbiology and Biotechnology
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    • v.20 no.7
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    • pp.1092-1100
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    • 2010
  • Bacteria, fungi, and protozoa inhabiting the rumen, the largest chamber of the ruminants' stomach, release large quantities of hydrogen during the fermentation of carbohydrates. The hydrogen is used by coexisting methanogens to produce methane in energy-yielding processes. This work shows, for the first time, a fundamental possibility of using a hydrogen-rich fermentation gas produced by selected rumen ciliates to feed a low-temperature hydrogen fuel cell. A biohydrogen fuel cell (BHFC) was constructed consisting of (i) a bioreactor, in which a hydrogen-rich gas was produced from glucose by rumen ciliates, mainly of the Isotrichidae family, deprived of intra- and extracellular bacteria, methanogens, and fungi; and (ii) a chemical fuel cell of the polymer-electrolyte type (PEFC). The fuel cell was used as a tester of the technical applicability of the fermentation gas produced by the rumen ciliates for power generation. The average estimated hydrogen yield was ca. 1.15 mol $H_2$ per mole of fermented glucose. The BHFC performance was equal to the performance of the PEFC running on pure hydrogen. No fuel cell poisoning effects were detected. A maximum power density of $1.66\;kW/m^2$ (PEFC geometric area) was obtained at room temperature. The maximum volumetric power density was $128\;W/m^3$ but the coulombic efficiency was only ca. 3.8%. The configuration of the bioreactor limited the continuous operation time of this BHFC to ca. 14 h.