• 제목/요약/키워드: Cell biovolume

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Lugol's Iodine Solution 첨가 후 보존 기간별 남조류 세포부피 변화 및 수축비를 이용한 생세포 부피 산정 (Effect of Lugol's Iodine Preservation on Cyanobacterial Biovolume and Estimate of Live Cell Biovolume Using Shrinkage Ratio)

  • 박혜경;이현제;이혜진;신라영
    • 한국물환경학회지
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    • 제34권4호
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    • pp.375-381
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    • 2018
  • The monitoring of phytoplankton biomass and community structure is essential as a first step to control the harmful cyanobacterial blooms in freshwater systems, such as seen in rivers and lakes, due to the process of eutrophication and climate change. In order to quantify the biomass of phytoplankton with a wide range in size and shape, the measurement of cell biovolume along with cell density is required for a comprehensive review on this issue. However, most routine monitoring programs preserve the gathered phytoplankton samples before analysis using chemical additives, because of the constraint of time and the number of samples. The purpose of this study was to investigate the cell biovolume change characteristics of six cyanobacterial species, which are common bloom-causing cyanobacteria in the Nakdong River, after the preservation with Lugol's iodine solution. All species showed a statistically significant difference after the addition of Lugol's iodine solution compared to the live cell biovolume, and the cell biovolume decreased to the level of 34.0 ~ 56.3 % at maximum in each species after the preservation. The nonlinear regression models for determining the shrinkage ratio by a preservation period were derived by using the cell biovolume measured until 180 days preservation of each target species, and the equation to convert the cell biovolume measured after preservation for a certain period to the cell biovolume of viable cell was derived using that formula. The conversion equation derived from this study can be used to estimate the actual cell biovolume in the natural environment at the time of sampling, by using the measured biovolume after the preservation in the phytoplankton monitoring. Moreover this is expected to contribute to the final interpretation of the water quality and aquatic ecosystem impacts due to the cyanobacterial blooms.

군산 인근 해역에서 종속영양 세균의 분포, 평균체적 및 세포외 효소활성력 (Distribution, Biovolume and Extracellular Enzyme Activities of Heterotrophic Bacteria in the Sea near Kunsan,Korea)

  • 이건형;김재원;김정희
    • The Korean Journal of Ecology
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    • 제17권1호
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    • pp.79-90
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    • 1994
  • 1991년 11월부터 1992년 8월까지 총 8회에 걸쳐 금강하구에서 고군산군도까지 6개 정점을 대상으로 종속영양 세균의 분포와 평군체적 및 세포와 효소활성을 측정하였다. 해양성 종속영양 세균은 1.0X103~5X105 c.f.u./ml의 범주에서 분포하였으며, 간균이 45~72%를 차지하였다. 또한 구균의 평균체적은 (7.69$pm$0.18)X10-2~(8.18$pm$0.38)X10-2$mu$m3, 간균은 (6.09$pm$0.29)X10-2~(7.72$pm$0.41)X10-2$mu$m3의 범주에서 변화하였다. 세포외 효소활성도를 측정한 결과, glucosidase의 활성은 0~3.49$mu$M/l/hr, chitinase의 활성은 0~1.25$mu$M/l/hr, phosphatase는 0~11.95$mu$M/l/hr, amylase는 0~3.80$mu$M/l/hr의 범주에서 측정되어 세포외 효소활성은 여름철에 높은 값을 보였으며, 측정효소중 phosphatase의 활성이 가장 높았다. 종속영양 세균의 분포와 세포외 효소활성은 수온과 유입되는 유기물의 양과 밀접한 관계가 있었으며, 세균의 체적의 크기는 세포외 효소 활성과는 직접적인 관계가 없었다.

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Image Analysis of Bacterial Cell Size by Diurnal Changes in Lake Soyang, Korea

  • Choi, Seung-Ik;Ahn, Tae-Seok;Kato, Ken-Ji
    • Journal of Microbiology
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    • 제34권4호
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    • pp.300-304
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    • 1996
  • To define the effects of zooplankton and phytoplankton to bacteria, bacterial numbers, frequency of dividing cells (FDC) and size distribution were performed with image analysis in the surface layer of Lake Soyang. In August 1992, when Anabaena was blooming, the bacterial number increased at daytime. Bacterial numbers and FDC value had a negative correlation (r = 0.83, P < 0.01). Bacterial size spectrums were dynamically changed during the day and night, especially the small bacteria less than $0.5\;{\mu}m^3$. Meanwhile, in October, after the bloom, the bacterial number was only one third of that in August, even though the FDC was higher than that in August. The bacterial numbers of small size class dropped at 13:00. But the size spectrums were relatively constant during the night time. These results suggest that the bacterial growth was tightly coupled with phytoplankton during Anabaena bloom. And after the bloom, the bacterial number was controlled grazing activity of zooplankton at daytime.

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High-Throughput In Vitro Screening of Changed Algal Community Structure Using the PhotoBiobox

  • Cho, Dae-Hyun;Cho, Kichul;Heo, Jina;Kim, Urim;Lee, Yong Jae;Choi, Dong-Yun;Yoo, Chan;Kim, Hee-Sik;Bae, Seunghee
    • Journal of Microbiology and Biotechnology
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    • 제30권11호
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    • pp.1785-1791
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    • 2020
  • In a previous study, the sequential optimization and regulation of environmental parameters using the PhotoBiobox were demonstrated with high-throughput screening tests. In this study, we estimated changes in the biovolume-based composition of a polyculture built in vitro and composed of three algal strains: Chlorella sp., Scenedesmus sp., and Parachlorella sp. We performed this work using the PhotoBiobox under different temperatures (10-36℃) and light intensities (50-700 μmol m-2 s-1) in air and in 5% CO2. In 5% CO2, Chlorella sp. exhibited better adaptation to high temperatures than in air conditions. Pearson's correlation analysis showed that the composition of Parachlorella sp. was highly related to temperature whereas Chlorella sp. and Scenedesmus sp. showed negative correlations in both air and 5% CO2. Furthermore, light intensity slightly affected the composition of Scenedesmus sp., whereas no significant effect was observed in other species. Based on these results, it is speculated that temperature is an important factor in influencing changes in algal polyculture community structure (PCS). These results further confirm that the PhotoBiobox is a convenient and available tool for performance of lab-scale experiments on PCS changes. The application of the PhotoBiobox in PCS studies will provide new insight into polyculture-based ecology.

식물플랑크톤 군집의 개체수, 생체량, chlorophyll $a$의 상관성; 인천, 통영, 울산 해역을 중심으로 (Correlations between Cell Abundance, Bio-volume and Chlorophyll $a$ Concentration of Phytoplankton Communities in Coastal Waters of Incheon, Tongyeong and Ulsan of Korea)

  • 주형민;이진환;정승원
    • 환경생물
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    • 제29권4호
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    • pp.312-320
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    • 2011
  • 본 연구는 해양 식물플랑크톤의 개체수 및 생체량과 chlorophyll $a$ 농도간의 연관성을 파악하기 위하여 2000년부터 2010년까지 인천, 통영, 울산 해역의 34개 정점에서 1160개 시료를 분석하였다. 연구결과 개체수와 chlorophyll $a$ 농도가 생체량과 chlorophyll $a$ 농도보다 높은 상관성을 보여 우리나라 연안해역에서는 1차 생산의 분석에 있어서 개체수 지표를 사용하는 것이 생체량을 사용하는 것보다 더 효율적일 것으로 판단된다. 그러나 좀 더 자세한 결과를 도출하기 위해서는 시기별로 보다 많고, 정확한 생체량 데이터를 확보하고, 시기별 chlorophyll 함량 자료의 축적과 함께 국내 연안에서 적용 가능한 모델 구축이 반드시 필요하다.

대구지역 인공저수지 조류의 계절별 천이 (Seasonal Succession of Algae in Artificial Reservoirs in Daegu City)

  • 이찬형;정윤숙;신상희;이순애;김용혜;홍성희
    • 한국미생물·생명공학회지
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    • 제30권3호
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    • pp.282-286
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    • 2002
  • 대구지역 인공저수지 조류군집의 계절적 천이와 수질을 조사하여 상호관련성을 알아보고자 하였다. 조류의 계절적 천이는 규조류$\longrightarrow$녹조류$\longrightarrow$남조류$\longrightarrow$규조류$\longrightarrow$규조류$\longrightarrow$남조류$\longrightarrow$남조류$\longrightarrow$남조류의 순서다. 이러한 천이순서는 다른 지역에서의 경향과는 다른 두 저수지의 특징적인 현상으로 국지적 환경조건에 의해 조류의 천이가 영향을 받는 것 같다. 2001년은 여름철 발생한 남조류가 저수온시기까지 남아있는 것으로 보아 저수지의 영양상태가 갈수록 악화되는 것으로 판단된다 조류현존량과 수질항목과의 상관분석에서는 대부분의 경우 상관계수가 낮게 나타났다. Chlorophyll-a는 수질항목과의 상관분석에서는 상관계수가 높게 나타났지만, 조류 현존량과의 상관계수는 낮게 나타난점은 조류현존량은 세포수만 나타낼뿐 조류종이나 영양상태에 따른 세포체적이 차이가 있음을 고려하지 않음도 원인이 될것으로 사료됨으로 세포수와 세포체적을 고려한 조류의 군집구조를 파악하는 방식으로의 접근이 요구된다.

낙동강 하구생태계의 세균 생물량과 이차생산성 (Bacterial Biomass and Secondary Productivity in Naktong River Estuary)

  • 송성주;권오섭;이혜주;이진애;김영의
    • 미생물학회지
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    • 제32권3호
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    • pp.238-244
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    • 1994
  • 부영양화된 낙동강 하구역에서 세균에 의한 용존 유기물의 이용능을 조사하고 이에 미치는 환경요인의 영향을 파악하기 위하여 미생물학적 요인과 물리화학적 환경요인의 연중 변화를 분석하였다. 총세균수는 0.33 ~ $2.09{\times}10^7$ cells/ml의 변화폭을 나타냈으며, 오염부하량이 큰 정점에서 종속영양세균의 개체수와 비례하였다. 세균이 체적과 생물량은 각각 0.064~0.156 ${\mu}m^3$/cell, 0.163~1.036 ${\mu}g$-C/ml을 나타냈다. 세균의 이차생산성은 0.24~60.86 ${\mu}g$-C/l/h의 범위로 측정되었으며, 부영양화 지표로 사용되는 환경인자와 밀접한 관계를 보였다. 담수역에 속하는 정점에서의 세균생산성의 계절별 변화는 동계에 최고치를, 하계에 최저치를 나타내어 갈수기의 오염부하량 증가와 밀접한 상관성을 보였다. 해수역에 속하는 다대의 경우 동계에 낮은 값을, 하계에 높은 값을 나타내고, 환경요인 중 엽록소 a, 수은 등과 밀접한 상관성을 보였다 (r>0.5, p<0.05). 이로 미루어 낙동강 하구의 하구언 상류역에서는 주위에서 유입되는 유기물이, 하구언 하류역에서는 식물성 플랑크톤의 광합성 산무링 세균의 이차생산성에 미치는 주 영양물질임을 암시하고 있다.

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낙동강하구 연안수역의 세균 생산성과 환경요인의 영향 (Heterotrophic Bacterial Secondary Productivity and Effect of Environmental Parameters is Naktong Embayment Korea)

  • 김미정;윤인길;정익교;권오섭
    • 미생물학회지
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    • 제36권2호
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    • pp.125-129
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    • 2000
  • 낙동강 연안 수역에서 세균의 생산성과 생산물량을 측정하여 미생물의 먹이고리에 기여하는 정도를 파악하기 위해 미생물학적 요인과 주요 환경요인을 측정하였다. 낙동강 연안 수계는 겨울철 갈수기에 유입되는 부영양화 담수의 영향을 많이 받았다. 종속영양세균과 총세균의 개체수는 각각$5.4{\times}10^2 ~ 3.2{\times}10^4, 2.2{\times}10^5 ~ 9.8{\times}10^5 $cells/ml이었으며, 세균의 평균 체적과 생물량은 각각 0.023~0.201$\mu$$m^3$/cell, 0.010~0.140$\mu$g-C/ml로 정점별, 계절별 차이는 크지 않았으나 체적과 총세균수가 반비례하였다. Thymidine pool size는 12.93~44.66nM로, 동계에 비해 하계에 세균이 이용하기 쉬운 pool로 이루어져 하계의세균 이차 생산성 측정치가 과소 평가될수 있음을 보여주고 있다. 종속영양세균의 이차생산성은 0.12~22.38$\mu$g-C/l/h의 변화 범위를 나타내었으며, 담수 유출부와 인접한 정점에서 가장 낮은 값을 보였다. 동계에 세균 생산성의 분포는 엽록소 a 및 수온 변화와 밀접한 상관성을 보였으며, 또한 세균 생산량도 많은 영향을 미치는 것으로 나타나 본 조사 수역에서 측정된 세균 생산성의 상당 부분은 식물플랑크톤의 공합성 산물을 이용하는 세균 생물량의 증가임을 알 수 있다.

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Ecosysteme de I′Etang de Berre (Mediterranee nord-occidentale) : Caracteres Generales Physiques, Chimiques et Biologiques

  • Kim, Ki-Tai
    • 환경생물
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    • 제22권2호
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    • pp.247-258
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    • 2004
  • Climatological, hydrological and planktonical research studies, measurements of primary production and photosynthetic efficiency from December 1976 to December 1978 have been carried out in two brackish lakes: Lake Etang de Berre and Lake Etang de Vaine located in the French Mediterranean coast, in the region of Carry-le-Rouet located on the north-west Mediterranean near Marseilles, and in fresh water inflows from 4 Rivers (Touloubre, Durance, Arc, Durancole) to Lake Etang de Berre. Physico-chemical parameters were measured for this study: water temperature, salinity, density, pH, alcalinity, dissolved oxygen (% saturation), phosphate, nitrate, nitrite, silicate etc. Diverse biological parameters were also studied: photosynthetic pigments, phaeopigments, specific composition and biomass of phytoplankton, primary pelagic production etc. Climatical factors were studied: air-temperature, solar-radiation, evaporation, direction (including strength) of winds, precipitation and freshwater volume of the four rivers. The changes in Lake ‘Etang de Berre’ ecosystem depend on the quality of the water in the Durance River, and on the effects of seawater near the entrance of the Caronte Canal. The water quality of the lake varies horizontally and vertically as a result of atmospheric phenomena, maritime currents and tides. The distribution of water temperatures is generally heterogeneous. Southeasterly winds and the Northeasterly Mistral wind are important in the origins of circulated and mixed water masses. These winds are both frequent and strong. They have, as a result, a great effect on the water environment of Lake Etang de Berre. In theory, the annual precipitation in this region is well over eight times the water mass of the lake. The water of the Durance River flows into Lake Etang de Berre through the EDF Canal, amounting to 90% of the precipitation. However, reduction of rainfall in dry seasons has a serious effect on the hydrological characteristics of the lake. The temperature in the winter is partially caused by the low temperature of fresh water, particularly that of the Durance River. The hydrological season of fresh and brackish water is about one month ahead of the hydrological season of sea water in its vicinity. The salinity of Lake Etang de Berre runs approximately 3$\textperthousand$, except at lower levels and near the entrance to the Caronte Canal. However, when the volume of the Durance River water is reduced in the summer and fall, the salinity rises to 15$\textperthousand$. In the lake, the ratio of fresh water to sea water is six to one (6:1). The large quantities of seston conveyed by rivers, particularly the Durance diversion, strongly reduce the transparency in the brackish waters. Although the amount of sunshine is also notable, transparency is slight because of the large amount of seston, carried chiefly by Tripton in the fresh water of the Durance River. Therefore, photosynthesis generally occurs only in the surface layer. The transparency progressively increases from freshwater to open seawater, as mineral particles sink to the bottom (about 1.7kg $m^{-2}a^{-1}$ on the average in brackish lakes). The concentration of dissolved oxygen and the rate of oxygen saturation in seawater (Carry-le-Rouet) ranged from 5.0 to 6.0 $m\ell$ㆍ.$1^{-1}$, and from 95 to 105%, respectively. The amount of dissolved oxygen in Etang de Berre oscillated between 2.9 and 268.3%. The monographs of phosphate, nitrate, nitrite and silicate were published as a part of a study on the ecology of phytoplankton in these environments. Horizontal and vertical distributions of these nutriments were studied in detail. The recent diversion of the Durance River into Lake Etang de Berre has effected a fundamental change in this formerly marine environment, which has had a great impact in its plankton populations. A total of 182 taxa were identified, including 111 Bacillariophyceae, 44 Chlorophyceae, and 15 Cyanophyceae. The most abundant species are small freshwater algae, mainly Chlorophyceae. The average density is about $10^{8}$ cells $1^{-1}$ in Lake Etang de Berre, and about double that amount in Lake Etang de Vaine. Differences in phytoplankton abundance and composition at the various stations or at various depths are slight. Cell biovolume V (equivalent to true biomass), plasma volume VP (‘useful’ biomass) and, simultaneously. the cell surface area S and S/V ratio through the measurement of cell dimensions were computed as the parameters of phytoplankton productivity and metabolism. Pigment concentrations are generally very high on account of phytoplankton blooms by Cyanophyceae, Chlorophyceae and Cryptophyceae. On the other hand, in freshwaters and marine waters, pigment concentrations are comparatively low and stable, showing slight annual variation. The variations of ATP concentration were closely related to those of chlorophyll a and phytoplankton blooms only in marine waters. The carbon uptake rates ranged between 38 and 1091 mg$Cm^{-2}d^{-1}$, with an average surface value of 256 mg; water-column carbon-uptake rates ranged between 240 and 2310 mg$Cm^{-2}d^{-1}$, with an average of 810, representing 290 mg$Cm^{-2}$, per year 45 000 tons per year of photosynthetized carbon for the whole lake. Gross photosynthetic production measured by the method of Ryther was studied over a 2-year period. The values obtained from marine water(Carry-le-Rouet) ranged from 23 to 2 337 mg$Cm^{-2}d^{-1}$, with a weighted average of 319, representing about 110 gCm$^{-2}$ per year. The values in brakish water (Etang de Berre) ranged from 14 to 1778 mg$Cm^{-2}d^{-1}$, with a weighted average of 682, representing 250 mg$Cm^{-2}$ per year and 38 400 tons per year of photosynthesized carbon for the whole lake.