• 한국발생생물학회지:발생과생식
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• 제19권3호
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• pp.127-134
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• 2015

The influence of triploidization on cell and nucleus size characteristics of the same tissues of erythrocyte, retina, kidney, hepatocyte and midgut epithelium in marine medaka, Oryzias dancena has been determined histologically. Induced triploid fish are produced by cold shock treatments. Likewise, the size of horizontal cell nucleus in inner nuclear layer of retina, ganglion cell nucleus in ganglion cell layer of retina, proximal tubule cell of kidney, hepatocytes and nuclear height of midgut epithelium all appear to be significantly larger than diploid (P<0.05). On the other hand, retina thickness is larger in diploid than induced triploid (P<0.05). Induced triploid shows low density of cell number. Results of this study suggest that same characteristics in the induced triploid exhibiting larger cells and nucleus sizes with fewer number of cells than the diploid can be useful criteria for the distinction between diploid and induced triploid, and also the ploidy level in marine medaka.

• Journal of Chest Surgery
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• 제44권2호
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• pp.159-164
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• 2011

Background: Renal cell carcinoma has shown less response to systemic therapies including chemotherapy, radiation, and immunotherapy than other cancers. Surgery has therefore become an important treatment tool. The protocol for treatment is the same for pulmonary metastasis of renal cell carcinoma. We performed surgery for pulmonary metastatic renal cell carcinomas and analyzed the results. Materials and Methods: We retrospectively analyzed 15 patients who had undergone pulmonary metastasectomy from renal cell carcinoma at our hospital from January 2005 to December 2009. Results: No patients had extrathoracic metastatsis. The mean age was 60.2 years (range 35~73). There were 12 male and 3 female patients. The number of synchronous and metachronous patients were 8 and 7, respectively. The mean survival times of synchronous and metachronous patients were 32.6 and 42.9 months, respectively. 6 patients had single lesions and 9 patients had multiple (more than 3) lesions. The surgical procedures included wedge resection (10), lobectomy (2), wedge resection with segmentectomy (2), and segmentectomy (1). Median observation and survival time were 54.1 and 34.9 months. The 1-year and 3-year survival rates were 80% and 50%, respectively. Conclusion: Pulmonary resection for pulmonary metastatic renal cell carcinoma was found to be a safe and effective treatment modality when complete resection was performed.

• 대한두경부종양학회지
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• 제25권1호
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• pp.33-35
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• 2009

Purpose : Granular cell tumour(GCT) is a rare benign tumour that can arise in anywhere throughout the body. Histopathological diagnosis of malignancy is difficult. We report a case of a granular cell tumor which developed on lower lip. Methods : A 58-year-old male had a palpable mass and whitish plaque in the oral mucosa for three months. Results : Under local anasthesia, the tumor was removed through wide excision including normal skin margin. Immediate reconstruction using local flap was done. Pathologic observation showed nests of polygonal cells with abundant granular, eosinophilic cytoplasm and round nuclei. Immunohistopathologic staining showed positive reaction on S-100 protein Conclusion : The results support the hypothesis that granular cell tumor is derived from Schwann cells. We report here on a case of granular cell tumor of lower lip that was successfully treated with radical resection.

• Journal of Radiation Protection and Research
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• 제34권1호
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• pp.15-24
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• 2009

The effect of low doses of ultraviolet (UV) irradiation on morphology changes of cell has been studied based on the observation of the cell length. It was shown that UV-irradiated cell has different behavior in comparison with non-irradiated cell. From the histogram of cell-length distribution, it was confirmed that cell cycle of non irradiated cell was 28 hours, and that cell cycle of irradiated cell with dose of $20\;Jm^{-2}$ was delayed (39 hours), while irradiated cell with $40\;Jm^{-2}$ and $60\;Jm^{-2}$ did not divide and kept growing continuously. It was supposed that in case of $20\;Jm^{-2}$ of irradiation dose, the cell cycle was delayed because the checkpoint worked in order to repair DNA damage induced by generation of pyrimidine dimer, reactive oxygen species and so on. It was also supposed that in case of $40\;Jm^{-2}$ and $60\;Jm^{-2}$ of irradiation dose, overgrowth was induced because the checkpoint was not worked well. The morphology of overgrown cell was similar to that of normally senescent cell. Therefore, it was considered that cell senescence was accelerated by UV irradiation with irradiation doses of $40\;Jm^{-2}$ and $60\;Jm^{-2}$.

• Journal of the Korean Wood Science and Technology
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• 제34권4호
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• pp.9-14
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• 2006

PEG처리재의 치수안정성 기구를 조사하기 위해 에너지분산스펙트럼분석법(SEM-EDS)을 이용하여 세포벽 내에 존재하는 PEG분자들의 분포를 분석하였다. 주사전자현미경으로 관찰한 결과, 무처리재의 세포들은 건조에 의한 수축이 발생하여 세포들이 찌그러진 형태를 나타냈지만, PEG처리재의 세포는 세포벽이 무처리재에 비해 다소 팽윤되었고, 세포들은 거의 원형을 유지하고 있었다. SEM-EDS법으로 분석한 결과, 세포벽에 K이온이 고르게 분포되어 있는 것이 관찰되었다. 따라서 PEG분자는 세포벽 내까지 침투하여 목재의 치수안정성 증대에 기여하는 것이 확인되었다.

• 한국동물학회지
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• 제16권2호
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• pp.109-118
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• 1973

동면전, 중, 후 및 활동기 동안 조선산개구리(Rana amurensis coreana Okada)의 위장관 점막내에 출현하는 enterochromaffin cell의 내분비성 분비과립의 변화를 형태학적으로 관찰하여 다음과 같은 결과를 얻었다. 1. 활동기 및 동면전, 중, 후기의 위장관 점막상피 내 enterochromaffin cell의 출현비율은 1: 1.5 :0.36 : 0.9로서 본 세포의 수는 활동기군에 비하여 동면전기군은 유의하게 증가하였으며 동면 중기군은 유의하게 감소하였다. 2. 활동기 및 동면전기군의 EC cell은 대체로 그 세포질내에 분비과립이 차있는 다과립성 및 중자등과립성 세포가 대다수였으나 동면중기 및 후기군에서는 희과립세포가 현저히 증가되었다. 3. EC cell의 형태학적 변화는 다른 신진대사 특히 내분비적 활성의 약화로 야기되는 생체의 적응현상에서 온 결과라고 추측된다.

• 대기
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• 제17권4호
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• pp.435-453
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• 2007

KEOP (Korea Enhanced Observing Period)-2004 intensive summer observation was carried out from 20 June to 5 July 2004 over the Southwestern part of the Korean peninsula. In this study, the effects of KEOP-2004 intensive observation data on the simulation of precipitation system are investigated using KLAPS (Korea Local Analysis and Prediction System) and PSU/NCAR MM5. Three precipitation cases during the intensive observation are selected for detailed analysis. In addition to the control experiments using the traditional data for its initial and boundary conditions, two sensitivity experiments using KEOP data with and without Jindo radar are performed. Although it is hard to find a clear and consistent improvement in the verification score (threat score), it is found that the KEOP data play a role in improving the position and intensity of the simulated precipitation system. The experiments started at 00 and 12 UTC show more positive effect than those of 06 and 18 UTC. The effect of Jindo radar is dependent on the case. It plays a significant role in the heavy rain cases related to a mesoscale low over Changma front and the landing of a Typhoon. KEOP data produce more strong difference in the 06/18 UTC experiments than in 00/12 UTC, but give more positive effects in 00/12 UTC experiments. One of the possible explanations for this is that : KEOP data could properly correct the atmosphere around them when there are certain amounts of data, while gives excessive effect to the atmospheric field when there are few data. CRA analysis supports this reasoning. According to the CRA (Contiguous Rain Area) analysis, KEOP data in 00/12 UTC experiments improve only the surrounding area, resulting in essentially same precipitation system so the effects remain only in each convective cell rather than the system itself. On the other hand, KEOP data modify the precipitation system itself in 06/18 UTC experiments. Therefore the effects become amplified with time integration.

• 전력전자학회논문지
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• 제16권5호
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• pp.521-531
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• 2011

본 논문은 그림자 영향을 고려한 PV(Photovoltaic) 시스템의 VPO(Variable Perturbation & Observation)MPPT(Maximum Power Point Tracking) 제어를 제시한다. 태양전지의 출력 특성은 비선형이고 온도, 일사량 및 그림자의 영향을 많이 받는다. MPPT 제어는 태양광발전 시스템의 출력 및 효율을 증가시키기 위한 매우 중요한 기술이다. 종래의 PO(Perturbation & Observation)와 IC(Incremental conductance) 등은 지속적인 자려진동에 의해 MPP(Maximum Power Point)를 찾는 방법으로 그림자 영향에 의해 출력이 급격하게 변할 경우 MPPT 제어를 수행하지 못한다. 이러한 문제점을 해결하기 위해 출력 변동에 따라 스텝 값이 변하는 새로운 제어 알고리즘을 제시한다. 제시한 알고리즘은 일사량, 온도 및 그림자 영향에 대해 종래의 제어 알고리즘과 응답특성을 비교하고 이를 통해 제시한 알고리즘의 타당성을 입증한다.

• Toxicological Research
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• 제21권1호
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• pp.77-85
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• 2005

Cadmium is an environmental pollutant exposed from contaminated foods or cigarette smoking and known to cause oxidative damage in organs. We investigated the cadmium-induced apoptosis and cell arrest in human breast cancer cells, MCF-7 cells and MDA-MB-231 cells. Obvious apoptotic cell death was shown in CdCl₂ 100 μM treatment for 12 hr, which were determined by DAPI staining and flow cytometric analysis. In cell cycle analysis, MCF-7 cells and MDA-MB-231 cells were arrested in S phase and G2/M phase respectively. These could be explained by the induction of cell cycle inhibitory protein, p21/sup Waf1/Cip1/ and p27/sup Kip1/, expression and reduction of cyclin/Cdk complexes in both cell lines. The decreased expression of cyclin A and Cdk2 in MCF-7 cells and cyclin B1 and Cdc2 in MDA-MB-231 cells were consistent with the flow cytometric observation. p-ERK expression was increased dose-dependent manner in both cell lines. It suggests that ERK MAPK pathway are involved in cadmium-induced cell cycle arrest and apoptosis. Moreover, cotreatment of zinc (100 μM, 12 hr) recovered the cadmium-induced cell arrest in both cells, which shows cadmium-induced oxidative stress mediates apoptosis and cell cycle arrest in human breast cancer cells.

• International Journal of Oral Biology
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• 제32권3호
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• pp.113-118
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• 2007

Treatment of oral cancers with chemotherapeutic agents become evaluated as an effective method to reduce cancer cell proliferation. Anti-proliferative and anti-oral cancer activities of momordin I on oral cancer cells were evaluated in this study. Momordin I was originally purified from a natural product, Ampelopsis radix and showed the antiproliferative activity against oral carcinoma, KB cells. Obtained $IC_{50}$ value was approximately $10.4{\mu}g/ml$. Time-and dose-dependent chromosomal DNA fragmentations were observed in momordin I-treated KB cells. Flow cytometry analysis showed time-dependent apoptotic cell appearance after treatment of momordin I. Approximately 18.6% apoptotic cells were observed at 72 hours after $20{\mu}g/ml$ of momordin I treatment. These observation were consistent with the results obtained in DNA fragmentation analysis. These data suggest that momordin I has anti-proliferative effect and induces cell death in KB cells through apoptosis.