• Title/Summary/Keyword: Cell Force Measurement

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Development of A New Micro-fabricated AFM Probe for the Measurement of Biomaterials by using the Precision Glass Bead Supply Unit (글래스비드 정밀공급기구에 의한 바이오재료평가용 AFM프로브의 개발)

  • Kweon, H.K.;Lin, J.
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.13 no.6
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    • pp.30-36
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    • 2014
  • Many different cells types have been found to be highly sensitive to mechanical force imposed by their surroundings. The cellular response to external mechanical forces has very important effects on numerous biological phenomena. In spite of its importance in biological processes, the cell adhesion force remains difficult to measure quantitatively at the cellular level. In this paper, to enhance quantitative measurements of cell adhesive interactions, a new attaching system and a method in which a glass bead can be attached to an AFM cantilever was designed and fabricated, and the degree of range displacement was controlled in the system. In an experiment, the movement of the stage in the attaching system and the attaching process were measured. The effectiveness of this system was confirmed as well in the experiment. In addition, through a commercial AFM system, the spring constant of the modified AFM probe could be measured.

Stiffness Comparison of Tissue Phantoms using Optical Coherence Elastography without a Load Cell

  • Chae, Yu-Gyeong;Park, Eun-Kee;Jeon, Min Yong;Jeon, Byeong-Hwan;Ahn, Yeh-Chan
    • Current Optics and Photonics
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    • v.1 no.1
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    • pp.17-22
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    • 2017
  • Mechanical property of tissue is closely related to diseases such as breast cancer, prostate cancer, cirrhosis of the liver, and atherosclerosis. Therefore measurement of tissue mechanical property is important for a better diagnosis. Ultrasound elastography has been developed as a diagnostic modality for a number of diseases that maps mechanical property of tissue. Optical coherence elastography (OCE) has a higher spatial resolution than ultrasound elastography. OCE, therefore, could be a great help for early diagnosis. In this study, we made tissue phantoms and measured their compressive moduli with a rheometer measuring the response to applied force. Uniaxial strain of the tissue phantom was also measured with OCE by using cross-correlation of speckles and compared with the results from the rheometer. In order to compare stiffness of tissue phantoms by OCE, the applied force should be measured in addition to the strain. We, however, did not use a load cell that directly measures the applied force for each sample. Instead, we utilized one silicone film (called as reference phantom) for all OCE measurements that indirectly indicated the amount of the applied force by deformation. Therefore, all measurements were based on displacement, which was natural and effective for image-based elastography such as OCE.

Obtaining Design Characteristics of Lever-linked Roberval Mechanism through Weighing Method (무게측정방식에 따른 Lever-linked Roberval Mechanism의 설계특성)

  • An, Ji Yun;Ahn, Jung Hwan;Lee, Gil Seung;Kim, Hwa Young
    • Journal of Sensor Science and Technology
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    • v.29 no.5
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    • pp.336-341
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    • 2020
  • The deflection and null balance methods are used for precision force measurement in the precision industry. Since both methods are based on deformation, the performance of the load cell mechanism is important. In this study, the design variables were obtained via the free body diagram of a lever-linked Roberval mechanism (combined with a flexible hinge link and a Roberval mechanism), and the design characteristics were analyzed according to the weight method. Based on the design characteristics, the optimal design was conducted according to the weight method and FEM was used to verify its reliability.

Force Test of the Linear Induction Motor for Magnetic Levitation Train (자기부상열차용 유도형 리니어 모터의 작용력 시험)

  • Jang, S.S.;Chang, K.W.;Lee, S.H.;Kim, B.S.;Sung, H.H.;Cho, H.J.
    • Proceedings of the KIEE Conference
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    • 2001.07b
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    • pp.611-613
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    • 2001
  • In this paper, the force test of arch-type LIM is treated. The method of direct measurement is used load-cell on 3-component force guage. Indirect method is used search coil on LIM. PC-based data aquistion system include two method and measure the force in driving state. Measured data of direct and indirect method are analyzed and compared.

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Adhesion Strength Measurement of Chondrocyte (연골세포 부착력 평가)

  • Lee K. Y.;Park S. K.;Shin Deahwan;Park J. C.
    • Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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    • 2004.11a
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    • pp.362-366
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    • 2004
  • Quantitative evaluation of substrates for cells is essential to understanding cell-material adhesive interaction and it is also necessary for the development of new biomaterials. Many cells on adhesive molecules will form an organization of actin into bundles and production of the large, highly organized structures termed focal adhesions. To better understand adhesion formations between cells and substrata, we have quantified the force required to displace attached cell. we allowed rabbit knee chondrocyte to attach on a substratum of microscope slide glass. Our results demonstrate that a force is required to detach cells is changed according to detachment time variation.

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Corrosion of Quartz Crystal Marine Sensors in Sea Water (항만센서용 수정진동자의 해수에 의한 부식)

  • 최광재;장상목;김영한
    • Journal of Korean Port Research
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    • v.12 no.2
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    • pp.323-328
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    • 1998
  • A quartz crystal analyzer is utilized to monitor the corrosion process of an aluminum surface of a quartz crystal for marine sensor by sea water. A quartz crystal having 2000 $\AA$ of aluminum layer is installed in a specially designed cell and is in contact with sea water imitated electrolyte solution. While a constant potential is applied to the cell, the resonant frequency and resonant resistance are simultaneously measured using the quartz crystal analyzer. In addition, surface topographs are taken with an atomic force microscope(AFM) and the element analysis of the surface is conducted using an energy dispersive X-ray spectrometer(EDX). The simultaneous measurement of resonant frequency and resonant resistance during the corrosion process explains the change of surface structure caused by the corrosion. The variation of resonant frequency addresses the amount surface metal dissolution. As a conclusion, it is found that a simple measurement using the quartz crystal analyzer can replace the complex monitoring employing large equipments in the investigation of a corrosion process of sensor surface.

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Design of Solenoid Actuator for FCV Cylinder Valve Considering Structural Safety (구조 안전성을 고려한 수소 연료 전지차 용기 밸브의 솔레노이드 액추에이터 설계)

  • Lee, Hyo Ryeol;Ahn, Jung Hwan;Shin, Jin Oh;Kim, Hwa Young
    • Journal of the Korean Society of Manufacturing Technology Engineers
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    • v.25 no.3
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    • pp.157-163
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    • 2016
  • Green vehicles include electric vehicles, natural gas vehicles, fuel cell vehicles (FCV), and vehicles running on fuel such as a biodiesel or an ethanol blend. An FCV is equipped with a cylinder valve installed in an ultra-high pressure vessel to control the hydrogen flow. For this purpose, an optimum design of the solenoid actuator is necessary to ensure reliability when driving an FCV. In this study, an electromagnetic field analysis for ensuring reliable operation of the solenoid actuator was conducted by using Maxwell V15. The electromagnetic field analysis was performed by magnetostatic technique, according to the distance between magnetic poles in order to predict the attraction force. Finally, the attraction force was validated through comparison between the Maxwell results and measurement results. From the results, the error of attraction force ranged from 4.53 % to 9.05 % at testing conditions.

A Study on the Measurement of Load Torque by the Field Coil Current in an Eddy Current Dynamometer (와전류 동력계에서 계자전류를 이용한 부하토크의 측정에 관한 연구)

  • Mun, Byung-Su;Lee, Eung-Suk;Lee, Hyung-Seok
    • Journal of the Korean Society for Precision Engineering
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    • v.18 no.10
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    • pp.84-92
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    • 2001
  • Commercial eddy current dynamometers control the torque of rotating body, power supply machine, with the field coil current being operated as a braking force. In this paper, we studied the relation between field coil current and torque load of eddy current dynamometer. By the torque measuring analysis of eddy current dynamometer it is linear relation between the brake force measured from a torque meter which is installed at the case of dynamometer and the multiplied shaft rpm by the squares of field coil current (N$\times$I$^2$). To prove the relation, it was experimented and showed that the torque operated by the rotating body can be measured with the shaft rpm and the field coil current of the eddy current dynamometer. This result shows a possibility that eddy current dynamometer can measure the torque of rotating body without a torque measuring device. such as load cell.

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ELECTRO-MICROSCOPE BASED 3D PLANT CELL IMAGE PROCESSING METHOD

  • Lee, Choong-Ho;Umeda Mikio;Takesi Sugimoto
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 2000.11b
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    • pp.227-235
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    • 2000
  • Agricultural products are easily deformable its shape because of some external forces. However, these force behavior is difficult to measure quantitatively. Until now, many researches on the mechanical property was performed with various methods such as material testing, chemical analysis and non-destructive methods. In order to investigate force behavior on the cellular unit of agricultural products, electro-microscope based 3D image processing method will contribute to analysis of plant cells behavior. Before image measurement of plant cells, plant sample was cut off cross-sectioned area in a size of almost 300-400 ${\mu}$ m units using the micron thickness device, and some of preprocessing procedure was performed with fixing and dyeing. However, the wall structure of plant cell is closely neighbor each other, it is necessary to separate its boundary pixel. Therefore, image merging and shrinking algorithm was adopted to avoid disconnection. After then, boundary pixel was traced through thinning algorithm. Each image from the electro-microscope has a information of x,y position and its height along the z axis cross sectioned image plane. 3D image was constructed using the continuous image combination. Major feature was acquired from a fault image and measured area, thickness of cell wall, shape and unit cell volume. The shape of plant cell was consist of multiple facet shape. Through this measured information, it is possible to construct for structure shape of unit plant cell. This micro unit image processing techniques will contribute to the filed of agricultural mechanical property and will use to construct unit cell model of each agricultural products and information of boundary will use for finite element analysis on unit cell image.

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Development of 4 Types of Fuel Cell's Blower (연료전지 블로어 4기종 국산화 개발)

  • Tak, Bong-Yeol;Kim, Chan-Gyu;Lee, So-A;Jang, Chun-Man
    • 한국신재생에너지학회:학술대회논문집
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    • 2011.05a
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    • pp.91-91
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    • 2011
  • This paper describes development procedure of the four types of fuel cell's blowers: pressurized fuel blower, selective oxidation air blower, cathode air blower, and burner air blower. Diaphragm blowers having two heads are selected to maintain force balance when the rotating arms are moving by the driving motor. Dimensions of a diaphragm cavity is designed according to the optimal design procedure using numerical simulation and experimental measurement. Experimental apparatus is designed by considering the bower characteristics having low flow rate and high pressure. Test blower is operated by a diaphragm, which has suction and discharge port on the top of the blower. For analyzing the internal flow of the blower, three-dimensional Navier-Stokes analysis is introduced in the present study. Throughout the optimal design of the blowers, blower performance is enhanced by reducing the unbalance motion of the rotating arm and loss region in the diaphragm cavity.

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