• Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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• v.6 no.3
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• pp.383-391
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• 2016

Ballast water provides stability and manoeuvrability to a ship. Foreign harmful aquatic organisms, which were transferred by ballast water, cause disturbing ecosystem. In order to minimize transference of foreign harmful aquatic organisms, IMO(International Maritime Organization) adopted the International Convention for the Control and Management of Ship's Ballast Water and Sediments in 2004. If the convention take effect, a port authority might need to check that ballast water is properly disposed of. In this paper, we propose a method of counting harmful aquatic organisms in ballast water thorough image processing. We extracted three samples from the ballast water that had been collected at Busan port in Korea. Then we made three grey-scale images from each sample as experimental data. We made a comparison between the proposed method and CellProfiler which is a well known cell-counting program based on image processing. Setting of CellProfiler is empirically chosen from the result of cell count by an expert. After finding a proper threshold for each image at which the result is similar to that of CellProfiler, we used the average value as the final threshold. Our experimental results showed that the proposed method is simple but about ten times faster than CellProfiler without loss of the output quality.

• 제어로봇시스템학회:학술대회논문집
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• 2003.10a
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• pp.2441-2444
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• 2003

This paper describes fabrication of a micro cell counter integrated with an oxygen micropump and Sephadex G-25 beads counting experiment. The device utilized a phototransistor, microwindow, and light source of microscope for beads detection. Microheater and microchannel were used for pumping and guiding of beads to the microwindow. Counting capability of the device was tested with a peristaltic pump and the measured signals (${\sim}10\;mV$) with oscilloscope showed peak shape when beads passed the microwindow. Pumping of beads by the oxygen micropump was carried out by heating paraffin, which enveloped manganese dioxide (catalyst), to trigger the decomposition of hydrogen peroxide into water and oxygen. It lasted for 5 min with $7\;{\mu}l$ of wt. 30 % hydrogen peroxide. Beads counting by oxygen micropump showed peaks ($2{\sim}20\;mV$) with $30\;{\mu}l$ of beads sample and the number of peaks by magnitude was acquired.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.8
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• pp.3397-3401
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• 2014

Determining cell quantity is a common problem in cytology research and anti-tumor drug development. A simple and low-cost method was developed to determine monolayer and adherent-growth cell quantities. The cell nucleus is located in the cytoplasm, and is independent. Thus, the nucleus cannot make contact even if the cell density is heavy. This phenomenon is the foundation of accurate cell-nucleus recognition. The cell nucleus is easily recognizable in images after fluorescent staining because it is independent. A one-to-one relationship exists between the nucleus and the cell; therefore, this method can be used to determine the quantity of proliferating cells. Results indicated that the activity of the histone deacetylase inhibitor Z1 was effective after this method was used. The nude-mouse xenograft model also revealed the potent anti-tumor activity of Z1. This research presents a new anti-tumor-drug evaluation method.

• Journal of Biomedical Engineering Research
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• v.5 no.1
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• pp.69-76
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• 1984

An automatic white blood cell differential counting system was developed, which consists of feature extractor, main control computer, auto focus and search part and data acquisition part. This system is used as a clinical instrument whose purpose is to classify white blood cell images. It may also be used for other binary image processing.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.28 no.8 s.227
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• pp.1159-1165
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• 2004

This paper describes fabrication of a micro cell counter integrated with an oxygen micropump and counting experiment with Sephadex G-25 beads ($70{\sim}100\;{\mu}m$). The pumping part consisted of a microheater, catalyst (manganese dioxide) enveloped with paraffin, hydrogen peroxide, and microchannel, and the counting part consisted of collimated light, a microwindow, and a phototransistor including an external circuit. The micropump generated oxygen gas by decomposing hydrogen peroxide with manganese dioxide, which was initiated by melting the paraffin with the microheater, and pumped beads in the microchannel. When the beads passed the microwindow, they shaded the collimated light and changed the illumination on the phototransistor, which caused the current variation in the circuit. The signals, according to the bead size, reached up to 22 mV with noise level of 2 mV during 50 seconds and the numbers of peaks were analyzed by magnitude.

• Food Science and Biotechnology
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• v.15 no.6
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• pp.866-870
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• 2006

The maintenance of physiological activity during dilution is very critical for the accurate enumeration of Vibrio spp. in marine samples. We investigated the effect of various diluents on the recovery of Vibrio parahaemolyticus using the direct plate counting and most probable number (MPN) methods. The effects of NaCl (0.85 and 3%) and pH (from 6.6 to 7.4) in diluents based on distilled water or phosphate buffered saline (PBS) were evaluated with three V. parahaemolyticus strains. PBS-3% NaCl (pH 6.6), as opposed to PBS, was the most effective diluent at maintaining viable cell numbers up to 2 log CFU/g during dilution for direct plate counting using on thiosulfate citrate bile salts sucrose (TCBS) selective agar, as well as minimizing the difference in cell numbers between TCBS and non-selective nutrient agar. It also increased counts of V parahaemolyticus inoculated into oysters relative to PBS (p<0.01), suggesting that PBS-3% NaCl (PH 6.6) can reduce the problem of underestimating V. parhaemolyticus counts using PBS alone.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.15 no.8
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• pp.648-658
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• 1990

There are many methods for speed up counting in speech recongition. A multiple processing method is the one way to achieve the aim using systolic array. This arithmetic operation by the array is achieved pipelining skill. And the operation is multiprocessing by processing element(PE) that is incresing counting efficiencies. The DTW PE cell is seperated into three large blocks. "MIN" is the one block for counting accumulated minimum distance, "ADD" block calculated these minimum distances, and "ABS" seeks for the absolut values to the total sum of local distances. We have accomplished circuit design and verification about the "ADD" and "ABS" blocks, and performed total layout '||'&'||' DRC(design rule check) using 3um CMOS N-Well rule base.le check) using 3$\mu$m CMOS N-Well rule base.

• Journal of IKEEE
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• v.24 no.1
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• pp.333-338
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• 2020

This paper proposes a method of estimating the SOC(State of Charge) of a battery cell using a neural network algorithm. To this, we implement a battery SOC estimation simulator and derive input and output data for neural network learning through charge and discharge experiments at various temperatures. Finally, the performance of the battery SOC estimation is analyzed by comparing with the experimental value by Ah-counting using Matlab/Simulink program and confirmed that the error rate can be reduced to less than 3%.

• Journal of the Korea Computer Industry Society
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• v.2 no.11
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• pp.1395-1406
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• 2001

In order to cover a widespread service range, terrestrial/satellite-mixed network is being combined with terrestrial ATM network. This dissertation analyzes and investigates several previously existent CDV compensation methods in order to compensate CDV arising from interfacing satellite TDMA and ATM. Specifically to supplement the problems of timestamp and cell number counting methods, new Variable Timestamp method for CDV compensation is proposed. To evaluate the proposed method, MMPP(Markov Modulated Poisson Process), which can express VBR service very well, is selected as a cell input traffic model of terrestrial transmitting earth station. After several simulation, it is also confirmed that CDV compensation capability for VBR services is very superior to the cell number counting method. In this case, as the timestamp number Nts increases, CDV compensation capability increases, and the CDV distribution length is reduced.

• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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• 2006.06b
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• pp.401-411
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• 2006

The cationic chitosan is prepared in this paper. This kind of cationic chitosan is a good retention aid for bleached hardwood pulp, the filler retention increased by 33.0% when the cationic chitosan (DS=1.27) dosage was 0.05%. Because many of the materials used in papermaking process are excellent microbiological nutrients, these nutrients will result in the growth of bacteria; uncontrolled growth of bacteria and fungi in the papermaking process adversely affects machine runnability. According to the standard methods of microbe growth inhibition test, cell counting was conducted after proper cultivated time. This paper explored the factors that affecting the cationic chitosan's antibacterial effect.