• Title/Summary/Keyword: Cell Buffer

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On-line Monitoring of IPTG Induction for Recombinant Protein Production Using an Automatic pH Control Signal

  • Hur Won;Chung Yoon-Keun
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.10 no.4
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    • pp.304-308
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    • 2005
  • The response of IPTG induction was investigated through the monitoring of the alkali consumption rate and buffer capacity during the cultivation of recombinant E. coli BL21 (DE3) harboring the plasmid pRSET-LacZ under the control of lac promoter. The rate of alkali consumption increased along with cell growth, but declined suddenly after approximately 0.2 h of IPTG induction. The buffer capacity also declined after 0.9 h of IPTG induction. The profile of buffer capacity seems to correlate with the level of acetate production. The IPTG response was monitored only when introduced into the mid-exponential phase of bacterial cell growth. The minimum concentration of IPTG for induction, which was found out to be 0.1 mM, can also be monitored on-line and in-situ. Therefore, the on-line monitoring of alkali consumption rate and buffer capacity can be an indicator of the metabolic shift initiated by IPTG supplement, as well as for the physiological state of cell growth.

박막태양전지 TCO/P 버퍼층 활성화를 위한 P-layer 최적화 Simulation

  • Jang, Ju-Yeon;Baek, Seung-Sin;Kim, Hyeon-Yeop;Lee, Jun-Sin
    • Proceedings of the Korean Vacuum Society Conference
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    • 2011.02a
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    • pp.91-91
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    • 2011
  • 박막태양전지의 높은 효율개선을 위해 TCO층과 p-layer 사이에 buffer layer를 넣어 Voc와 FF를 개선하는 연구가 진행되고 있다. 이에 buffer layer의 활성화 정도를 높이기 위해 p-layer을 최적화 시키고자한다. 이 실험에서 a-Si:B에 N2O를 도핑시켜 Bandgap Energy 2.0 eV, Activation Energy 0.4 eV인 a-SiOx:B 막을 제작하여 buffer layer로 사용하였고 이 buffer layer에 의한 cell의 효율 향상을 최적화 하기위해 ASA simulation을 이용해 p-layer의 Bandgap Energy와 Activation Energy를 가변 하여 보았다. 실험결과 p-layer의 Bandgap Energy 1.95 eV에서 buffer layer와 p-layer사이에서의 barrier가 최소가 됨을 확인 할 수 있었고 Actication Energy 0.5 eV에서 가장 높은 Voc를 가짐을 알 수 있었다. 본 연구를 통해 p-layer의 Bandgap Energy 1.95 eV, Activation Energy 0.5 eV에서 buffer layer를 활성화시키기 위한 p-layer의 최적화 조건을 구현해 볼 수 있었다.

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Effects of pH, Buffer System and Lactate on the Simulated Ischemia-reperfusion Injury of H9c2 Cardiac Myocytes

  • Lee, Jun-Whee;Lee, Hye-Kyung;Kim, Hae-Won;Kim, Young-Hoon
    • The Korean Journal of Physiology and Pharmacology
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    • v.11 no.2
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    • pp.45-55
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    • 2007
  • We elucidated the effects of various components of ischemic medium on the outcome of simulated ischemia-reperfusion injury. Hypoxia for up to 12 hours induced neither apoptotic bodies nor LDH release. However, reoxygenation after 6 or 12 hours of hypoxia resulted in a marked LDH release along with morphological changes compatible with oncotic cell death. H9c2 cells were then subjected to 6 hours of simulated ischemia by exposing them to modified hypoxic glucose-free Krebs-Henseleit buffer. Lowered pH (pH 6.4) of simulated-ischemic buffer resulted in the generation of apoptotic bodies during ischemia, with no concomitant LDH release. The degree of reperfusion-induced LDH release was not affected by the pH of ischemic buffer. Removal of sodium bicarbonate from the simulated ischemic buffer markedly increased cellular damages during both the simulated ischemia and reperfusion. Addition of lactate to the simulated ischemic buffer increased apoptotic cell death during the simulated ischemia. Most importantly, concomitant acidosis and high lactate concentration in ischemic buffer augmented the reperfusion-induced oncotic cell death. These results confirmed the influences of acidosis, bicarbonate deprivation and lactate on the progression and outcome of the simulated ischemia-reperfusion, and also demonstrated that concomitant acidosis and high lactate concentration in simulated ischemic buffer contribute to the development of reperfusion injury.

Performance Analysis of ATM Switch Using Priority Control by Cell Transfer Ratio (셀 전송비율에 의한 우선순위 제어방식을 사용한 ATM 스위치의 성능 분석)

  • 박원기;김영선;최형진
    • Journal of the Korean Institute of Telematics and Electronics A
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    • v.32A no.12
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    • pp.9-24
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    • 1995
  • In this paper, we proposed and analysed two kinds of priority control mechanism to archive the cell loss rate requirement and the delay requirement of each class. The service classes of our concern are the high time priority class(class 1) and the high loss priority class(class 2). Two kinds of priority control mechanism is divided by the method of storing the arriving class 2 cell in buffer on case of buffer full. The first one is the method which discarding the arriving class 2 cell, the second one is the mothod which storing the arriving class 2 cell on behalf of pushing out the class 1 cell in buffer. In the proposed priority schemes, one cell of the class 1 is transmitted whenever the maximum K cells of the class 2 is transmitted on case of transmitting the class 1 cell and the class 2 cell sequentially. In this paper, we analysed the cell loss rate and the mean cell delay for each class of the proposed priority scheme by using the Markov chain. The analytical results show that the characteristic of the mean cell delay becomes better for the class 1 cell and that of the cell loss rate becomes better for the class 2 cell by selecting properly the cell transfer ratio according to the condition of input traffic.

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Decalcomanie of flat-tubular segmented SOFC cell bytranscription-method and output characteristics according to buffer Layer

  • Gu, Ja-Bin;Choe, Byeong-Hyeon;Ji, Mi-Jeong;Lee, Mi-Jae;An, Yong-Tae;Hwang, Hae-Jin
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2012.05a
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    • pp.93.2-93.2
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    • 2012
  • 연료전지는 전기화학반응을 이용한 발전 장치로서 기존 장치에 비하여 발전 효율이 높아 화석연료를 사용하면서 현재 당면 과제인 $CO_2$ 배출량 절감이 가능하고, 환경 보전성이 우수하여 미래의 전원으로 많은 연구개발이 진행되고 있다. 특히 제3세대 연료전지라 불리는 고체산화물 연료전지(Solid Oxide Fuel Cell이하 SOFC)는 고가의 외부 개질 장치 없이도 연료가 갖는 화학에너지를 연소과정 없이, 공기와 $H_2$, CO, $CH_4$와 같은 환원성 가스를 공급받아 $600{\sim}1000^{\circ}C$에서 전기화학적 반응을 통하여 직접 전기를 얻는 방식이며, 낮은 소음과 진동으로 인하여 온 사이트(On-site) 발전이 가능한 장점이 있는 연료전지이다. Decalcomanie는 전사용지에 Screen printing하여 건조 후 coating하는 방법으로 기존의 여러 coating 방법보다 다전지셀 제작이나 Buffer layer의 적용이 용이하고, 소재의 크기나 두께조절이 간편하며, 구성층의 표면조도나 굴곡에 대응이 용이한 방법이다. 새로운 Decalcomanie를 사용하여 평관형 다전지식 SOFC Cell 제작 및 각 Buffer layer에 적용, Screen printing법과 동일한 Cell 제조 후 MPD와 Impedance 분석을 통하여 Support 위에 전사지를 이용, 적층한 Cell의 전기화학적 특성에 관하여 분석하였다.

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Virtual System Buffer Model in Flexible Manufacturing Systems with an AGV System (AGVS를 포함한 FMS에서의 가상 시스템버퍼 모델에 관한 연구)

  • Kyung Sup Kim
    • Journal of the Korea Society for Simulation
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    • v.7 no.2
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    • pp.45-62
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    • 1998
  • This research is concerned with buffer management in a multi-cell FMS with an AGVS. Buffers in manufacturing systems are required to reduce blocking and starving caused by breakdowns, variability in process times, and diversity of part routing. Due to the high per unit buffer cost, which primarily consists of floor space and equipment costs, the total capacity of buffers in and FMS is very limited. Proper buffer management can provide a high system efficiency. This paper presents a buffer management model for a multi-cell FMS with an AGVS and a simulation study to compare the proposed model to a conventional buffer management model in a job shop FMS.

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A Fairness Improvement Algorithm using Dynamic Threshold in ATM-GFR Service (ATM-GFR 서비스에서 동적 임계치를 이용한 공평성 향상 알고리즘)

  • Kim, Nam-Hee;Kim, Byun-Gon
    • The KIPS Transactions:PartC
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    • v.10C no.3
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    • pp.305-310
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    • 2003
  • The performance of various GFR implementations has been recently studied due to the interest to provide bandwidth guarantees with a simpler implementation than ABR in ATM networks. One of the important factors is buffer management for guaranteeing QoS in GFR service. An efficient buffer management algorithm is necessary to guarantee MCR for untagged cell in ATM switch. In this paper, we propose and evaluate a buffer management scheme to provide the GFR service guarantees. The proposed scheme can control the cell discarding for fairness in each VC, and compared with Double-EPD and DFBA. Our results show that the proposed buffer management with per-VC queuing achieves significant enhancement on goodputs and fairness index than those of existing methods.

Application of Buffer Layers for Back Contact in CdTe Thin Film Solar Cells

  • Chun, Seungju;Kim, Soo Min;Lee, Seunghun;Yang, Gwangseok;Kim, Jihyun;Kim, Donghwan
    • Proceedings of the Korean Vacuum Society Conference
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    • 2014.02a
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    • pp.318.2-318.2
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    • 2014
  • The high contact resistance is still one of the major issues to be resolved in CdS/CdTe thin film solar cells. CdTe/Metal Schottky contact induced a high contact resistance in CdS/CdTe solar cells. It has been reported that the work function of CdTe thin film is more than 5.7 eV. There has not been a suitable back contact metal, because CdTe thin film has a high work function. In a few decades, some buffer layer was reported to improve a back contact problem. Buffer layers which are Te, $Sb_2Te_3$, $Cu_2Te$, ZnTe:Cu and so on was inserted between CdTe and metal electrode. A formed buffer layers made a tunnel junction. Hole carriers which was excited in CdTe film by light absorption was transported from CdTe to back metal electrode. In this report, we reported the variation of solar cell performance with different buffer layer at the back contact of CdTe thin film solar cell.

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A Novel Hydrogen-reduced P-type Amorphous Silicon Oxide Buffer Layer for Highly Efficient Amorphous Silicon Thin Film Solar Cells (고효율 실리콘 박막태양전지를 위한 신규 수소저감형 비정질실리콘 산화막 버퍼층 개발)

  • Kang, Dong-Won
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.65 no.10
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    • pp.1702-1705
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    • 2016
  • We propose a novel hydrogen-reduced p-type amorphous silicon oxide buffer layer between $TiO_2$ antireflection layer and p-type silicon window layer of silicon thin film solar cells. This new buffer layer can protect underlying the $TiO_2$ by suppressing hydrogen plasma, which could be made by excluding $H_2$ gas introduction during plasma deposition. Amorphous silicon oxide thin film solar cells with employing the new buffer layer exhibited better conversion efficiency (8.10 %) compared with the standard cell (7.88 %) without the buffer layer. This new buffer layer can be processed in the same p-chamber with in-situ mode before depositing main p-type amorphous silicon oxide window layer. Comparing with state-of-the-art buffer layer of AZO/p-nc-SiOx:H, our new buffer layer can be processed with cost-effective, much simple process based on similar device performances.

The Cell Resequencing Buffer for the Cell Sequence Integrity Guarantee for the Cyclic Banyan Network (사이클릭 벤얀 망의 셀 순서 무결성 보장을 위한 셀 재배열 버퍼)

  • 박재현
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.41 no.9
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    • pp.73-80
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    • 2004
  • In this paper, we present the cell resequencing buffer to solve the cell sequence integrity problem of the Cyclic banyan network that is a high-performance fault-tolerant cell switch. By offering multiple paths between input ports and output ports, using the deflection self-routing, the Cyclic banyan switch offer high reliability, and it also solves congestion problem for the internal links of the switch. By the way, these multiple paths can be different lengths for each other. Therefore, the cells departing from an identical source port and arriving at an identical destination port can reach to the output port as the order that is different from the order arriving at input port. The proposed cell resequencing buffer is a hardware sliding window mechanism. to solve such cell sequence integrity problem. To calculate the size of sliding window that cause the prime cost of the presented device, we analyzed the distribution of the cell delay through the simulation analyses under traffic load that have a nonuniform address distribution that express tile Property of traffic of the Internet. Through these analyses, we found out that we can make a cell resequencing buffer by which the cell sequence integrity is to be secured, by using a, few of ordinary memory and control logic. The cell resequencing buffer presented in this paper can be used for other multiple paths switching networks.