• Biomolecules & Therapeutics
• /
• 제20권6호
• /
• pp.513-519
• /
• 2012

Although the immense efforts have been made for cancer prevention, early diagnosis, and treatment, cancer morbidity and mortality has not been decreased during last forty years. Especially, lung cancer is top-ranked in cancer-associated human death. Therefore, effective strategy is strongly required for the management of lung cancer. In the present study, we found that novel daphnane diterpenoids, yuanhualine (YL), yuanhuahine (YH) and yuanhuagine (YG) isolated from the flower of Daphne genkwa (Thymelaeaceae), exhibited potent anti-proliferative activities against human lung A549 cells with the $IC_{50}$ values of 7.0, 15.2 and 24.7 nM, respectively. Flow cytometric analysis revealed that the daphnane diterpenoids induced cell-cycle arrest in the G0/G1 as well as G2/M phase in A549 cells. The cell-cycle arrests were well correlated with the expression of checkpoint proteins including the up-regulation of cyclin-dependent kinase inhibitor p21 and p53 and down-regulation of cyclin A, cyclin B1, cyclin E, cyclin dependent kinase 4, cdc2, phosphorylation of Rb and cMyc expression. In the analysis of signal transduction molecules, the daphnane diterpenoids suppressed the activation of Akt, STAT3 and Src in human lung cancer cells. The daphnane diterpenoids also exerted the potent anti-proliferative activity against anticancer-drug resistant cancer cells including gemcitabine-resistant A549, gefitinib-, erlotinib-resistant H292 cells. Synergistic effects in the growth inhibition were also observed when yuanhualine was combined with gemcitabine, gefitinib or erlotinib in A549 cells. Taken together, these findings suggest that the novel daphnane diterpenoids might provide lead candidates for the development of therapeutic agents for human lung cancers.

• Molecules and Cells
• /
• 제22권2호
• /
• pp.146-153
• /
• 2006

Cell polarity is critical for the division, differentiation, migration, and signaling of eukaryotic cells. RAX2 of budding yeast encodes a membrane protein localized at the cell cortex that helps maintain the polarity of the bipolar pattern. Here, we designate SPAC6f6.06c as $rax2^+$ of Schizosaccharomyces pombe, based on its sequence homology with RAX2, and examine its function in cell polarity. S. pombe $rax2^+$ is not essential, but ${\Delta}rax2$ cells are slightly smaller and grow slower than wild type cells. During vegetative growth or arrest at G1 by mutation of cdc10, deletion of $rax2^+$ increases the number of cells failing old end growth just after division. In addition, this failure of old end growth is dramatically increased in ${\Delta}tea1{\Delta}rax2$, pointing to genetic interaction of $rax2^+$ with $tea1^+$. ${\Delta}rax2$ cells contain normal actin and microtubule cytoskeletons, but lack actin cables, and the polarity factor for3p is not properly localized at the growing tip. In ${\Delta}rax2$ cells, and endogenous rax2p is localized at the cell cortex of growing cell tips in an actin- and microtubule-dependent manner. However, ${\Delta}rax2$ cells show no defects in cell polarity during shmoo formation and conjugation. Taken together, these observations suggest that rax2p controls the cell polarity of fission yeast during vegetative growth by regulating for3p localization.

• 대한조선학회지
• /
• 제11권2호
• /
• pp.23-40
• /
• 1974

Semisubmersible 해양석유시추선의 기본설계에 필요한 파랑중(波浪中)에서 운동(運動)을 계산(計算)하는 이론적방법(理論的方法)을 제시하고 "MOHOLE"과 "SEDCO 1350-F" 석유시추선들의 운동(運動)을 해석하였다. 이 규칙파에서 운동계산을 불규칙해양파(波)에 적용하는 응용해석을 보여주었다. 현재 이론적 방법으로는 6자유도(自由度)의 운동을 해양파의 어떤 방향에 대해서도 정확히 계산할 수 있으며 계산의 정확성은 수조(水槽)에서의 모형선의 운동측정치와 실선(實船)의 운동측정치와 비교하여 증명되었다. 또 현재의 방법은 종전에 개발된 방법보다 더 일반적(一般的)인 경우를 다룰 수 있으며 결과치도 더 정확하다. 극소운동특성을 갖는 해양석유시추선과 부체(浮體)해양구조물의 설계는 경비가 비싸고 시간이 많이 드는 모형실험보다는 유체역학적(流體力學的) Parameters를 신속 정확히 자주 변경 검토해야 하는 기본설계단계에서는 정확한 이론적인 전자계산기에 의한 계산방법이 절실히 필요하다. 예상(豫想)과 같은 부가질량(附加質量)과 감쇠력(減衰力)은 Resonance 운동주기에서만 운동에 영향을 준다. 해양구조물에 작용하는 파력(波力)은 Froude-Krilov force, 부가질량(附加質量) 및 감쇠력(減衰力)과 Restoring force로 구성했으며 규칙파(規則波)에서의 6자유도(自由度) 운동방정식은 본 논문에 제시된 실험측정치(値)와 실험으로 정확도가 증명된 이론치(値)의 부가질량과 감쇠력 계수(係數)를 써서 풀었다. 규칙파(規則波)에서의 계산된 운동을 Pierson Moskowitz 해양파(海洋波) 스펙트럼과 linear superposition principle에 의해 불규칙해양파(不規則海洋波)에서의 운동을 계산하는데 사용했다. 불규칙파(不規則波)에서의 운동은 운동스펙트럼과 통계적 운동치로 나타냈다. 현재의 계산방법은 실제 기본설게에 사용되어 왔으며, 다른 응용분야는 파랑중(波浪中)에서의 파면(波面)과 Deck간(間)의 Clearance, 계류선(係留線)의 동장력(動張力)계산의 기본 Data 및 기본설계의 Draft 등 Parameters를 통(通)한 Optimum Design 등(等)이다. 파(波)의 한 방향(方向)에 대(對)한 전자계산기(電子計算機)(IBM 370 또는 CDC 6400)에 의한 운동계산은 10초(秒)미만밖에 안걸린다. 또 현재의 계산방법은 해양석유시추선뿐 아니라 이와 비슷한 부체(浮體)해양구조물과 Pipe-laying선(船) 또 Supply Boat설계(設計)에도 쓰여지고 있다.

• The Journal of Korean Physical Therapy
• /
• 제19권4호
• /
• pp.1-14
• /
• 2007

Background: It is generally accepted that skeletal muscle contraction is triggered by nerve impulse and intracellular $Ca^{2+}\;([Ca^{2+}]_i)$ released from intracellular $Ca^{2+}$ stores such as sarcoplasmic reticulum (SR). Specifically, this process, called excitation-contraction (E-C) coupling, takes place at intracellular junctions between the plasma membrane, the transverse (T) tubule L-type $Ca^{2+}$ channel (dihydropyridine-sensitive L-rype $Ca^{2+}$ channel, DHPR, also called tetrads), and the SR $Ca^{2+}$ release channel (ryanodine-sensitive $Ca^{2+}$ release channel, RyR, also called feet) of internal $Ca^{2+}$ stores in skeletal muscle cells. Furthermore, it has been reported that the $Ca^{2+-}$ dependent and -independent contraction determine the expression of skeletal muscle genes, thus providing a mechanism for tightly coupling the extent of muscle contraction to regulation of muscle plasticity-related excitation-transcription (E-T) coupling. Purpose: Expression and activity of plasticity-associated enzymes in gastrocnemius muscle strips have not been well studied, however. Methods: Therefore, in this study the expression and phosphorylation of E-C and E-T coupling-related mediators such as protein kinases, ROS(reactive oxygen species)- and apoptosis-related substances, and others in gastrocnemius muscles from rats was examined. Results: I found that expression and activity of MAPKs (mitogen-activated protein kinases, ERK1/2, p38MAPK, and SAPK/JNK), apoptotic proteins (cleaved caspase-3, cytochrome c, Ref-1, Bad), small GTP-binding proteins (RhoA and Cdc42), actin-binding protein (cofilin), PKC (protein kinase C) and $Ca^{2+}$ channel (transient receptor potential channel 6, TRPC6) was observed in rat gastrocnemius muscle strips. Conclusion: These results suggest that MAPKs, ROS- and apoptosis-related enzymes, cytoskeleton-regulated proteins, and $Ca^{2+}$ channel may in part functionally import in E-C and E-T coupling from rat skeletal muscles.

• 한국자원식물학회지
• /
• 제35권6호
• /
• pp.796-804
• /
• 2022

The demand for Chelidonium majus L. subsp. asiaticum H. Hara is expected to increase due to its pharmacological properties such as antibacterial, antioxidant, and anti-inflammatory effects. However, an effective propagation system for this species has not yet been established. This study was conducted to analyze the seed dormancy and germination characteristics of C. majus L. subsp. asiaticum H. Hara native to Korea and establish a mass propagation system. The dormancy type was primarily classified by analyzing the general information of the collected seeds. The seed dormancy breaking was investigated by comparing the effects of cold stratification (0, 2, 4, 8, 10, or 12 weeks) with warm stratification (S, summer temperature, 25/15℃) and intermediate temperature stratification (A, autumn temperature, 15/10℃) of alternating temperature stratification (S12-A4 or S12-A8 weeks). After dormancy break, 500 mg/L GA₃ and GA₄₊₇ treatment replaced cold stratification and improved seed germination. The results of this study are expected to provide basic data for future seed propagation and mass propagation by analyzing the dormancy and germination characteristics of C. majus L. subsp. asiaticum H. Hara seeds.

• 한국학교보건학회지
• /
• 제19권2호
• /
• pp.75-88
• /
• 2006

Purpose : This study are the secondary data of Global Youth Tobacco Survey(GYTS) developed by the WHO and United State Center for Disease Control(CDC) and Prevention to trace tobacco use among youth in youth in countries across the world. This study was carried out to serve for a basis for antismoking campaigns and to prevent adolescents from smoking by analyzing the smoking by analyzing the smoking realities factors. Methods : This study is a cross-sectional school-based survey, which employed a two-stage cluster sample design to produce a nationally representative sample of middle school students aged 13 to 15 years olds. Seventy-five schools were selected. All schools containing grade 1, 2 and 3 that contained 40 or students were included in the sampling frame. Results : The results of this study were as follows ; 1. The proportion of smoking students in middle school was 6.4% and the rate of smoking in male students(7.4%) was higher than it of in female students(5.5%). 2. 26.5% of all respondent had a experience to try smoke and the results showed that the rate of trying smoking in male students was increased by grade more than female students. 3. According to test of significance in regression analysis, the independent variables such as sex, school grade, smoking parents, friends distinction, pocket money and others showed statistical significance. 4. There was a statistical significance between smokers and nonsmokers in proportion to the completing the preventive education for nonsmoking(p=0.0023) and the acquirement of knowledge on smoking(p<.0001). 5. Finally there was a statistical significance between the exposure to anti-smoking campaign(p=0.0053) and tobacco advertisement in the mass media and the smoking(p=0.0036). Conclusion : All things considered, the health education for tobacco control in school need to be developed from an elementary school. I suggest that prevent smoking program in school need to be revitalized and be made more accessible for everyone who wish to quit. And the government should develop the regulation on total banning promoting cigarette advertising and smoking scene in the movie for young people.

• 기본간호학회지
• /
• 제25권1호
• /
• pp.46-57
• /
• 2018

Purpose: The purposes of the study were to examine the knowledge and practice about Middle East Respiratory Syndrome (MERS) isolation precaution, and to explore influencing factors of the isolation practice among hospital nurses. Methods: A total of 182 nurses were recruited from four general hospitals where MERS patients had been treated. The knowledge and practice of MERS isolation precaution were measured by the scales developed based on the CDC guidelines. The collected data were analyzed by SPSS/WIN 22 with descriptive statistics, T-test, One-way ANOVA, Pearson correlation coefficients, and hierachical multiple regression analysis. Results: The nurses were 30 years old in average, and half of them had less than 5 years of clinical experience. and knowledge on droplet precautions (${\beta}=.171$, p=.019) were the significant predictors, explaining 19.6% of variance in the MERS isolation guideline practice. Clinical experience (${\beta}=.225$, p=.002), working at infection unit (${\beta}=-.203$, p=.011). Conclusion: The knowledge on droplet precaution and general knowledge on MERS were the important modifiable factor to improve the MERS isolation guideline practice among hospital nurses, even after adjusting clinical experience and demographic variables. It is necessary to develop an efficient education program on specific guidelines for prevention and management of infection by improving the knowledge on infectious disease such as MERS as well as droplet precaution which are modifiable factors.

• 분석과학
• /
• 제6권1호
• /
• pp.65-75
• /
• 1993

오존층 파괴물질로 규명된 CDC 113의 대체 세정제를 수집하여 실험적인 방법으로 밀도, 표면장력, 굴절지수, 비점, pH, 점도, 인화점, 용해도를 측정하였다. 대체세정제는 크게 수계 세정제, 준수계 세정제, 알코올 및 케톤계 세정제, 할로겐 세정제로 나누어서 측정한 물성들을 비교하였다. 전자산업의 잔자회로기판(PCB)에 사용되는 flux의 주성분인 abietic acid와 각 세정제의 용해실험은 HPLC를 사용하여 용해도를 구하였다. 각 분류별 세정제는 장단점을 갖추고 있으며 최종 사용자는 물성에 의한 세정제의 효율성과 세정방법 뿐만 아니라 안정성, 경제성을 종합적으로 고려해야 한다. 본 연구의 목적은 시판중인 CFC 113의 대체 세정제를 수집하여 물성을 측정하고 비교함으로써 사용자가 원하는 최적의 대체 세정제를 선정하는 데 기본적인 자료를 제공하는 것이다.

• 대한예방한의학회지
• /
• 제14권3호
• /
• pp.107-115
• /
• 2010

Objectives : The purpose of this study is to investigate the efficacy of growth exercise program based on Korean medicine. Methods : We recruited fifty two elementary students in a rural area, and measured height, weight, BMI, WHR, and bone age. Questionnaire on sleep, exercise, symptoms based on Korean medicine was performed. Students exercised TaeGuk(太極) Health Gigong(氣功), a growth exercise program 11 times in 6 weeks. Results : Height, weight, BMI and WHR of the subject students were lower than those in the Korean CDC(Center for Disease Control and Prevention) growth chart. There was significantly negative correlation between time of sleep and bone age(p=0.028), but no correlation between time of exercise and bone age(p=0.668). According to diagnosis based on Korean medicine, many students were in weakness of spleen and lung, and bone age according to deficiency of organ showed no significant difference(p=0.774). Satisfaction, impact on time of exercise after program, ideal time for program and impact on reparticipation of program were strong in the questionnaire after growth exercise program(TaeGuk(太極) Health Gigong(氣功)). Conclusions : Korean medicine promotion of health has a salutary effect on elementary school students in rural area by collecting growth and health data and educating easy exercise based on Taichi.

• Journal of Microbiology and Biotechnology
• /
• 제26권6호
• /
• pp.1026-1034
• /
• 2016

In the current study, a total of 135 enterococci strains from different sources were screened for the presence of the enterocin-encoding genes entA, entP, entB, entL50A, and entL50B. The enterocin genes were present at different frequencies, with entA occurring the most frequently, followed by entP and entB; entL50A and L50B were not detected. The occurrence of single enterocin genes was higher than the occurrence of multiple enterocin gene combinations. The 80 isolates that harbor at least one enterocin-encoding gene (denoted "Gene⁺ strains") were screened for antimicrobial activity. A total of 82.5% of the Gene⁺ strains inhibited at least one of the indicator strains, and the isolates harboring multiple enterocin-encoding genes inhibited a larger number of indicator strains than isolates harboring a single gene. The indicator strains that exhibited growth inhibition included Listeria innocua strain CLIP 12612 (ATCC BAA-680), Listeria monocytogenes strain CDC 4555, Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923, S. aureus ATCC 29213, S. aureus ATCC 6538, Salmonella enteritidis ATCC 13076, Salmonella typhimurium strain UK-1 (ATCC 68169), and Escherichia coli BAC 49LT ETEC. Inhibition due to either bacteriophage lysis or cytolysin activity was excluded. The growth inhibition of antilisterial Gene⁺ strains was further tested under different culture conditions. Among the culture media formulations, the MRS agar medium supplemented with 2% (w/v) yeast extract was the best solidified medium for enterocin production. Our findings extend the current knowledge of enterocin-producing enterococci, which may have potential applications as biopreservatives in the food industry due to their capability of controlling food spoilage pathogens.