• 한국과학교육학회지
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• 제43권1호
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• pp.1-15
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• 2023

유전자변형생물체(LMO)의 사회적, 경제적 가치가 높아짐과 동시에, LMO가 인체와 환경에 미치는 위험성도 알려졌다. 따라서 LMO를 취급하는 모든 실험실은 LMO 연구시설에 관한 법령에서 요구하는 기준에 따라 LMO 연구시설을 마련해야 한다. 최근, 고등학교에서도 교육적 목적으로 LMO를 활용하는 실험 수업이 이루어지고 있는데, 이 경우에도 LMO 연구시설을 갖추는 것이 필요하다. 하지만 고등학교에서 LMO 연구시설 제도의 개념은 낯설고, 일반 연구시설과 같은 기준을 적용한 운영 지침은 현장 교사들에게 이를 마련하는 데 어려움으로 작용하고 있다. 이에 본 연구는 LMO 연구시설을 지속하여 운영한 3개의 고등학교 사례를 찾아, LMO 연구시설을 마련하는 과정에서 교사가 겪은 어려움과 그 원인을 탐색하고자 원인연쇄분석(causal chain analysis)을 실시하였다. 교사들이 겪는 어려움은 다음과 같이 요약할 수 있다. 첫째, '망설여지는 LMO 연구시설 신고'이다. 이는 '신고에 필요한 행정 업무'와 '학내 구성원 설득'이 어려움의 직접 원인이 되었다. 둘째, '제도적 사각지대에 있는 LMO 연구시설운영'이다. 이는 '유연하지 않은 연구시설 신고 및 폐쇄', '의료 폐기물처리', '현장과 맞지 않는 LMO 교육'이 어려움의 직접 원인이 되었다. 어려움에 대한 심층 원인을 탐색한 결과 'LMO 연구시설의 필요성에 대한 인식 부족'과 '학교 맥락에 대한 부족한 고려'가 중간 원인으로 나타났으며, 근본 원인으로 '교육현장과 운영기관 사이의 협력적 준비 부족'으로 나타났다. 이에 관하여 연구에 참여한 교사는 '개방형 실험실 운영'과 '노하우 공유의 기회 마련'을 고등학교의 LMO 연구시설 운영 활성화 전략으로 제언하였다. 앞으로 본 연구가 고등학교에 LMO 연구시설을 마련하고자 하는 교사나 학교가 참조할 수 있을 기초자료가 되기를 바란다.

• 한국건설관리학회논문집
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• 제6권3호
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• pp.144-155
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• 2005

1950년대 이후 주택수요의 급격한 증가와 낮은 주택투자로 인한 만성적 주택부족 사태는 정부 주도의 주택공급정책에 의존해 오고 있다. 주택시장이 수요와 공급에 의한 자율적 가격결정구조를 가지지 못하고 정부 정책에 따라 인위적으로 움직임으로써 주택가격은 폭등과 폭락을 반복하고, 아파트 미분양 등에 의한 건설사의 수익성 악화나 중소건설업체의 부도와 같은 문제점들이 발생하고 있다. 이는 건설기업이 주택시장의 구조적 문제점을 파악하고 정부의 정책에 시기 적절히 대처하지 못함으로써 주택시장적응력을 상실한 결과라 할 수 있다. 또한 정부도 주택시장의 가격에 의존한 단기적 정책 효과만을 의존함으로써 주택시장의 가격결정시스템을 약화시키는 결과를 초래한 것이다. 즉, 주택시장의 동태적 환경시스템이라는 숱을 보지 못하고 정부 정책에 의한 단기적 영향요인이라는 나무만을 봄으로써 나타난 결과라 할 수 있다. 따라서 본 연구는 주택 시장을 주택수요 영향변수와 주택공급 영향변수 그리고 그에 영향을 미치는 거시 환경적 영향변수들을 분류하고, 이들 간의 상호작용을 시스템 사고와 전통적 소비자 선택이론에 입각하여 연결함으로써 이에 의해 구성되는 인과순환구조를 구축하도록 한다. 이를 통해 주택시장이 가지는 동태성의 장기적인 형태를 피드백 루프의 존재를 통해 확인해 줌으로써 주택시장의 역동적 변화 원인을 규명하고자 한다.

• 인터넷정보학회논문지
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• 제13권5호
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• pp.65-75
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• 2012

조직시민행동이란 조직내의 공식적 역할이나 기능을 넘어서는 구성원들의 재량적 행동을 의미하며, 조직의 성과 제고에 상당한 기여를 하는 것으로 알려져 있다. 공급망관리(SCM) 시스템이 도입되면, 글로벌 표준, 프로세스 혁신, 핵심성과지표 등에 관한 정보가 여러 부서에 폭넓게 전달되어 구성원들의 흡수 및 혁신능력을 제고할 수 있다. 구성원들의 조직시민행동은 이런 과정을 활성화시켜 SCM 성과를 증진시키며 결과적으로 조직 혁신에 기여할 수 있다. 본 논문은 국내에서 비즈니스를 전개하고 있는 글로벌 제조 기업에 근무하고 있는 조직구성원의 흡수능력과 조직시민행동의식, SCM의 성과 및 혁신의도 간 인과관계 분석에 초점을 둔다. 122명으로부터 수집한 설문자료를 통계적으로 분석한 결과, 조직시민행동은 SCM 성과와 흡수능력에 긍정적 영향을 미치며, 흡수능력 또한 SCM 성과에 직접적 영향을 미치는 것으로 나타났다. 기대한 바와 같이 SCM 성과는 혁신의도의 유의한 선행요인으로 분석되었지만, 조직구성원의 흡수능력은 별다른 영향을 미치지 못하는 것으로 나타났다. 그 외 본 연구결과의 학술적, 실무적 시사점도 함께 제시하였다.

• The Plant Pathology Journal
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• 제38권6호
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• pp.629-636
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• 2022

Colletotrichum species is known as the major causal pathogen of red pepper anthracnose in Korea and various groups of fungicides are registered for the management of the disease. However, the consistent use of fungicides has resulted in the development of resistance in many red pepper-growing areas of Korea. Effective management of the occurrence of fungicide resistance depends on constant monitoring and early detection. Thus, in this study, various methods such as agar dilution method (ADM), gene sequencing, allele-specific polymerase chain reaction (PCR), and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were applied for the detection of benzimidazole resistance among 24 isolates of Colletotrichum acutatum s. lat. and Colletotrichum gloeosporioides s. lat. The result of the ADM showed that C. gloeosporioides s. lat. was classified into sensitive and resistant isolates to benomyl while C. acutatum s. lat. was insensitive at ≥1 ㎍/ml of benomyl. The sequence analysis of the β-tubulin gene showed the presence of a single nucleotide mutation at the 198th amino acid position of five isolates (16CACY14, 16CAYY19, 15HN5, 15KJ1, and 16CAYY7) of C. gloeosporioides s. lat. Allele-specific PCR and PCR-RFLP were used to detect point mutation at 198th amino acid position and this was done within a day unlike ADM which usually takes more than one week and thus saving time and resources that are essential in the fungicide resistance management in the field. Therefore, the molecular techniques established in this study can warrant early detection of benzimidazole fungicide resistance for the adoption of management strategies that can prevent yield losses among farmers.

• 경영과학
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• 제23권2호
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• pp.1-16
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• 2006

With the diffusion of the Internet, firms try to electronically collaborate with their partners in order to cut costs and gain profits. This, electronic Partnership, called 'Collaborative IT' is quite popular between large purchase enterprises and small-to-medium sized sub-contractors. This study investigates such relations. This study proposes three groups of research variables-interorganizational relationship, collaborative IT activity, and firm performance. the interorganizational relationship consists of trust, commitment, and asymmetry of commitment. Collaborative IT activity is composed of information sharing and workflow integration. The ultimate dependent variable is firm performance. It is hypothesized that the relationship factors influence the level of collaborative IT activity, while the latter in turn affects the firm performance. The relationship factors nay also directly affect the dependent variable. In addition, collaborative IT motive, as a moderating variable, may influence the causal relationship. By means of survey, ore hundred and eighty-two responses were obtained. Most sample companies are small-sized, in the manufacturing sector. The analysis of data reveals that both trust and commitment positively affects the level of collaborative IT activity, while asymmetry of commitment has negative effects. The workflow integration is significantly related with firm performance. Information sharing, however, has no signific3nt effects. Furthermore, asymmetry of commitment shows reverse relationship with firm performance. Collaborative IT motive works as a moderating variable between information sharing and firm performance. Finally, workflow integration is believed to mediate between relationship factors and firm performance.

• International Journal of Contents
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• 제12권4호
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• pp.31-44
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• 2016

Knowledge creation and management are regarded as critical success factors for an organization's survival in the knowledge era. As a process of knowledge acquisition and sharing, organizational learning mechanisms (OLMs) guide the learning function of organizations represented by its different learning activities. We examined a variety of learning processes that constitute OLMs. In this study, we aimed to capture the process and framework of OLMs and knowledge sharing and acquisition. Factors facilitating OLMs were investigated at three levels: individual, group, and organizational. The concept of an OLM has received some attention in the field of organizational learning, however, the relationship among the factors generating OLMs has not been empirically tested. As part of the ongoing discussion, we attempted a systemic approach for OLMs. OLMs can be represented by factors that are inherent to the organization's system; therefore, prior to empirically testing the OLM generating factor(s), evaluation of its organizational integration is required to determine effective treatment of each factor. Thus, we developed a framework to manage knowledge and proposed a method to numerically evaluate factors influencing the OLMs. Specifically, composite importance (CI) of the Decision-Making Trial and Evaluation Laboratory (DEMATEL) method was applied to explore the interaction effect of these factors based on systemic approach. The augmented matrix thus generated is expected to serve as a stochastic matrix of an absorbing Markov chain.

• Journal of Microbiology and Biotechnology
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• 제17권11호
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• pp.1765-1771
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• 2007

A polymerase chain reaction (PCR)-based method was developed to detect the DNA of Ralstonia solanacearum, the causal agent of bacterial wilt in various crop plants. One pair of primers (RALSF and RALSR), designed using cytochrome c1 signal peptide sequences specific to R. solanacearum, produced a PCR product of 932 bp from 13 isolates of R. solanacearum from several countries. The primer specificity was then tested using DNA from 21 isolates of Ralstonia, Pseudomonas, Burkholderia, Xanthomonas, and Fusarium oxysporum f. sp. dianthi. The specificity of the cytochrome c1 signal peptide sequences in R. solanacearum was further confirmed by a DNA-dot blot analysis. Moreover, the primer pair was able to detect the pathogen in artificially inoculated soil and tomato plants. Therefore, the present results indicate that the primer pair can be effectively used for the detection of R. solanacearum in soil and host plants.

• 대한간호학회지
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• 제40권5호
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• pp.662-675
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• 2010

Purpose: This ethnography was done to explore the meaning of illness in Korean Americans with chronic hepatitis B. Methods: The participants were 6 patients with chronic hepatitis B and 6 general informants who could provide relevant data. Data were collected from iterative fieldwork with ethnographic interviews within Korean communities in two cities in the United States. Data were analyzed using causal chain analysis developed by Wolcott. Results: The analyses revealed three meanings for the illness: hidden disease, intentionally hidden disease, and inevitably hidden disease. The contexts of meaning of illness included characteristics of the illness, social stigma, structure of health care system and communication patterns and discourse between health care providers and clients. Conclusion: The meaning of illness was based on folk illness concepts and constructed in the sociocultural context. Folk etiology, pathology and interpretation of one's symptoms were factors influencing illness behavior. These findings could be a cornerstone for culture specific care for Korean Americans with chronic hepatitis B.

• 한국정보시스템학회지:정보시스템연구
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• 제23권2호
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• pp.1-23
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• 2014

Companies are required to have adequate understanding and awareness of a partner company within the supply chain, together with the understanding of the imbalance consequent on power types. Therefore, this study intends to look into the power types and make clear their influence on confidence & commitment and causal relationship that is linked to SCM user satisfaction. The concrete research results are as follows. First, the result showed that none of the behavioral coercive, high-handed reward, and relative legitimacy in terms of Mediated power had a significant influence on confidence and commitment. Like this, the result explains that there is a limit to forcibleness power in forming a continuous, long-term relationship. Second, business expertise, professional information and value reference in terms of Non-Mediated power were all found to have a significant influence on confidence and commitment. This could be judged as having an intention of aggressively accepting a partner company's expertise, information strength and imitable culture, etc. Third, both confidence and commitment was found to have a significant influence on SCM user satisfaction.

• Plant Biotechnology Reports
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• 제2권1호
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• pp.13-20
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• 2008

Potato (Solanum tuberosum L.), one of the most important food crops, is susceptible to a number of devastating fungal pathogens in addition to bacterial and other pathogens. Producing disease-resistant cultivars has been an effective and useful strategy to combat the attack of pathogens. Potato was transformed with Agrobacterium tumefaciens strain EHA101 harboring chitinase, (ChiC) isolated from Streptomyces griseus strain HUT 6037 and bialaphos resistance (bar) genes in a binary plasmid vector, pEKH1. Polymerase chain reaction (PCR) analysis revealed that the ChiC and bar genes are integrated into the genome of transgenic plants. Different insertion sites of the transgenes (one to six sites for ChiC and three to seven for bar) were indicated by Southern blot analysis of genomic DNA from the transgenic plants. Expression of the ChiC gene at the messenger RNA (mRNA) level was confirmed by Northern blot analysis and that of the bar gene by herbicide resistance assay. The results obviously confirmed that the ChiC and bar genes are successfully integrated and expressed into the genome, resulting in the production of bialaphos-resistant transgenic plants. Disease-resistance assay of the in vitro and greenhouse-grown transgenic plants demonstrated enhanced resistance against the fungal pathogen Alternaria solani (causal agent of early blight).