• 한국축산식품학회지
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• 제29권6호
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• pp.668-672
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• 2009

This paper describes the differentiation between native Korean cattle (Hanwoo) and Holsteins or imported cattle using the real-time polymerase chain reaction (PCR) by targeting the sequence of the melanocortin 1 receptor (MC1R) gene. A rapid and accurate method was developed to identify Hanwoo by genotyping the DNA extracted from 295 commercial beef samples (obtained from 5 provinces in South Korea) labeled as Hanwoo beef. The results of real-time PCR assays for the proportions of Hanwoo were 84, 85.7, 95, 91.4, and 90% in the areas of Seoul, Joongbu, Youngnam, Honam, and Chungcheong, respectively. Thus, the beef samples from 295 butcher shops, which asserted to only sell Hanwoo, showed that 259 of 295 samples were of the Hanwoo beef gene type (T-type) and 36 of 295 samples were Holsteins of imported dairy cattle gene types (C-type or C/T type). In conclusion, the proportion of Hanwoo beef was 87.8% and the proportion of Holstein or imported dairy cattle meat was 12.2% (C-type: 9.8%, C/T-type: 2.4%). Generally, most consumers can not differentiate imported meat from Hanwoo beef. Therefore, Hanwoo beef and imported dairy cattle meat that is sold in butcher shops should have mandatory identification by using MC1R genotyping based on real-time PCR.

• Asian-Australasian Journal of Animal Sciences
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• 제32권8호
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• pp.1211-1218
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• 2019

Objective: The objective of this study was to determine the effects of age and gender (bull vs steer) on feeding behavior parameters in young beef cattle fed grass silage. Methods: The study was conducted on 180 young beef cattle at 7 to 18 mo of age. The experimental materials comprised 90 bulls produced by commercial crossing of Polish Holstein-Friesian cows with Charolais, Limousin and Hereford bulls (30 animals of each breed) and 90 steers of the same genotypes. The animals had ad libitum access to grass silage; the concentrate was fed separately, in feed stations. They received 28 g dry matter of concentrate per kg of metabolic body weight per day. Bunk visit data and silage intake for all experimental animals were recorded individually using the Roughage Intake Control system (5 feed bunks per 15 animals). Results: Age and gender (bull vs steer) exerted significant effects on the feeding behavior of young beef cattle. The frequency of bunk visits and meal frequency decreased, whereas the feeding rate of silage, and the average duration and size of a single meal increased with age (p<0.01). Bunk attendance and meal frequency were higher (p<0.01) in steers than in bulls (49.1 vs 37.4 visits/d, and 8.63 vs 7.99 meals/d, respectively). Daily feeding time was longer in steers than in bulls (102.3 vs 100.3 min/d, respectively), but the feeding rate of silage was lower in steers, and their meals were smaller in size and shorter in duration (p<0.01). Daily silage dry matter intake was higher (p<0.01) in bulls than in steers (4.62 vs 4.47 kg/d, respectively). Conclusion: The results of this study indicate that age and gender (bull vs steer) exerted significant effects on the feeding behavior of young beef cattle.

• 한국동물생명공학회지
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• 제37권4호
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• pp.226-230
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• 2022

The sheep can be reproduced by natural mating as well as applied reproductive biotechnology, embryo transfer (ET). However, this method in sheep is influenced by several factors such as season, photoperiod, latitude, temperature, nutrition, and breed. In addition, there is still less research on assisted reproductive technologies in small ruminants, compared to other livestock species such as cattle and pigs. Because there has been a need for an optimization and a continuous improvement of ET techniques in small ruminants. the main objective of this study was to evaluate the conception rate obtained after ET in Mongolian sheep (Dorper breed). After embryo recover, code 1 and 2 embryos (morula or blastocyst stage) for ET in the present study were 63% (63/100) and 24% (24/100), respectively. Then Each single embryo was transferred to a synchronized recipient who prepared by estrous synchronization protocol with fluorogestone acetate-cloprostenol sodium. The results demonstrated that an average conception rate and lambing rate was 35.6% (31/87) and 33.3% (29/87), respectively. Further study is still necessary, but these results indicated that single embryo of Mongolian sheep with the present protocol was enough to conducting ET when the genetically superior sheep were necessary to be expanded.

• Asian-Australasian Journal of Animal Sciences
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• 제14권8호
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• pp.1062-1066
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• 2001

Studies were carried out on forty young bulls of the Hariana breed (around 18 months of age) selected on the basis of their dam's milk yield at the animal farm of CCS HAU, Hisar during the period 1993-1999. Since animals showed variation in capacity to work over prolonged periods, they were subjected to regimes of carting without load and with 8 qtls. of load for three hours. The study revealed that maximum change took place in the pulse rate, followed by respiration rate and rectal temperature. The mean fatigue score for pulling an empty cart ranged between 1.66 to 2.20 after two hours work, and 2.36 to 2.73 after three hours work. For a cart loaded with 8 qtls., the corresponding ranges were 2.90 to 3.36 and 3.40 to 4.10, respectively. These results indicated that the animals under experiment had the capacity to pull moderate load (8 qtls.) for about two hours without showing any serious effect on the ability to recover and soon to work again.

• Asian-Australasian Journal of Animal Sciences
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• 제14권12호
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• pp.1655-1658
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• 2001

Bovine genome samples were collected from meat of three different beef breeds (Hanwoo, Holstein and imported beef breed) that are commercially merchandized in Korean beef market. Operon B (OPB)-kits were used as random primers (3, 7, 10, 11, 12, 14) in random amplified polymorphic DNA (RAPD) method on whole genome. Each primer provided characteristic bands that were highly polymorphic. Each single primer could provide relatively efficient polymorphic band patterns among breeds. However, use of two or more primers in combination is recommended to improve resolution of experiments with higher molecular weight bands of DNA. In our experiments, OPB-11 resolved well between beef cattle breeds and Holstein. And OPB-7, 12 and 14 could be combined with OPB-11 to identify Hanwoo beef from the other two kinds of beef.

• Journal of Animal Science and Technology
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• 제50권5호
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• pp.633-640
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• 2008

소의 모색 발현에 결정적인 역할을 수행하며 Extension 좌위에 암호화되어 있는 melanocortin- 1 receptor(MC1R) 유전자형을 변형된 염기서열이 증폭되게 제작된 이중 mismatch primer 쌍을 이용하여 PCR-RFLP 방법으로 분석하였다. 증폭된 PCR 절편들은 MC1R 유전자에서 모색 표현형과 직접적으로 연관되어 있어 중요하게 다루어지고 있는 세 가지 대립인자들(ED, E+, e)로 MspI-과 AluI-RFLP에 의해 성공적으로 구분되었다. MC1R 유전자형의 분포를 조사한 결과 제주흑우는 세 가지 대립인자가 모두 출현하였고, 황－적모색의 한우와 호피문의 칡소에서는 흑모색우성 대립인자 ED가 출현하지 않았다. 반면, 우성흑모색으로 알려진 두 소 품종 Holstein과 Angus는 ED 대립인자의 빈도가 96% 이상으로 조사되었다. 한우×Holstein F1과 한우×Angus F1은 모두 ED/e의 유전자형을 나타내었고, 표현형은 전신 흑색으로 확인되었다. 본 연구에서 고안한 이중 mismatch primer 쌍을 이용한 MC1R 유전자 증폭 절편에 대한 MspI-과 AluI-RFLP 조합은 소의 품종 특성 규명과 품종 식별에서 매우 중요한 유전자 표지인자 중 하나인 MC1R 유전자의 세 가지 대립인자를 식별하는 데 유용한 실험기법이 될 것으로 사료된다.

• 한국수정란이식학회지
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• 제28권3호
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• pp.207-213
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• 2013

This study was carried out to examine a molecular marker system for parentage test in Jeju Black cattle (JBC). Based on the preliminarily studies, we finally selected for construction of a novel genetic marker system for molecular traceability, identity test, breed certification, and parentage test in JBC and its related industrial populations. The genetic marker system had eight MS markers, five indel markers, and two single nucleotide polymorphisms (SNPs; g.G299T and g.del310G) within MC1R gene which is critical to verify the breed specific genotypes for coat color of JBC differing from those of exotic black cattle breeds such as Holstein and Angus. The results showed lower level of a combined non-exclusion probability for second parent (NE-P2) of $4.1202{\times}10^{-4}$ than those previously recommended by International Society of Animal Genetics (ISAG) of $5.000{\times}10^{-4}$ for parentage, and a combined non-exclusion probability for sib identity (NE-SI) of $2.679{\times}10^{-5}$. Parentage analysis has been successfully identified the JBC offspring in the indigenous population and cattle farms used the certified AI semens for production using the JBC-derived offspring for commercial beef. This combined molecular marker system will be helpful to supply genetic information for parentage test and traceability and to develop the molecular breeding system for improvement of animal productivity in JBC population.

• Journal of Animal Science and Technology
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• 제45권1호
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• pp.13-22
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• 2003

성장호르몬 유전자는 하나의 작은 공통 선조 유전자로부터 아주 오랜 기간동안 유전자 중복에 의해 진화되어 온 그룹들 중의 하나이다. 이들에 속하는 유전자들은 동물 종간에 구조적인 상동성과 기능적 공통성 등 유사성이 비교적 높게 나타난다. 이런 연구결과들을 근거로 하여 소 성장호르몬 유전자에서 아미노산을 암호화하는 영역으로부터 새로운 아미노산의 변이(missense mutation)를 검출하였고, 이 변이의 대립유전자 빈도는 소(cattle)의 종(species) 및 품종의 지리적 분포에 따라 일정한 경향 치를 보여 주었다. 한편 변경되어진 아미노산은 Tryptophan으로 이는 생물체에 존재하는 많은 단백질들을 구성하는 아미노산중에서도 그 출현빈도가 가장 낮은 것이다. 또한 검출된 변이는 성장호르몬이 그의 수용체와 강하게 결합하는 부위로서, 성장호르몬의 구조적 변이를 초래하여 수용체와의 결합이 비정상적으로 이루어져, 이후 성장호르몬이 표적세포로의 신호전달과 같은 역할을 제대로 수행치 못하게 되고, 이로 인하여 가축의 표현되어지는 경제형질에 영향을 미칠 것으로 추정된다. 그러므로 이러한 대립유전자를 보유하는 개체는 집단에서 제거하는 방법에 의한 개량이 가능할 것으로 사료된다.

• Journal of Animal Science and Technology
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• 제51권4호
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• pp.273-282
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• 2009

소 품종 판별을 위해 DNA 마커 정보는 품종을 구별하거나, 형질을 구분하는데 있어 꾸준히 이용되고 있다. 본 연구에서는 Finnzymes (DIAGNOSTICS)사의 Bovine Genotypes Kit Ver1.1/2.1을 농촌진흥청 국립축산과학원이 보유한 호주산 및 미국산 수입우 DNA 샘플 148두/국내산 육우 DNA 샘플(Holstein) 170두와 정읍지역 한우 DNA 샘플 177두에 적용하여 한우품종 식별력을 분석 하였다. Bovine Genotype Kit 1.1은 11개의 ISAG MS 마커로 이루어져 있으며, 여기에 5개 MS 마커가 추가된 ver2.1 Kit를 사용하여 집단별 유전자형 데이타를 구축하였고, MS Tool kit 분석 및 Phylip program 분석을 수행하여 Phylogenetic tree를 작성하였고, Genotype 분석 프로그램인 GeneClass 2.0 (INRA/France)을 이용하여 품종 식별력을 추정하였다. 분석 결과 95% 이상의 정확성을 가진 한우 식별력은 100%로 나타났고, 호주산 수입우 95.3%, 국내산 육우는 90%의 높은 식별력을 각각 나타내었다. 따라서 Finnzymes 사의 상용화된 16종의 MS 마커는 한우집단의 유전적 특징을 객관적으로 구분하여 수입쇠고기/젖소고기/한우쇠고기에서 간편하게 한우개체 및 품종식별에 활용될 수 있는 가능성과 특히 국내에서 비육된 육우(젖소)를 수입산 쇠고기로부터 식별할 수 있는 장점이 있을 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제29권5호
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• pp.624-630
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• 2016

Many studies have reported the frequency and distribution of haplogroups among various cattle breeds for verification of their origins and genetic diversity. In this study, 318 complete sequences of the mtDNA control region from four Korean cattle breeds were used for haplogroup classification. 71 polymorphic sites and 66 haplotypes were found in these sequences. Consistent with the genetic patterns in previous reports, four haplogroups (T1, T2, T3, and T4) were identified in Korean cattle breeds. In addition, T1a, T3a, and T3b sub-haplogroups were classified. In the phylogenetic tree, each haplogroup formed an independent cluster. The frequencies of T3, T4, T1 (containing T1a), and T2 were 66%, 16%, 10%, and 8%, respectively. Especially, the T1 haplogroup contained only one haplotype and a sample. All four haplogroups were found in Chikso, Jeju black and Hanwoo. However, only the T3 and T4 haplogroups appeared in Heugu, and most Chikso populations showed a partial of four haplogroups. These results will be useful for stable conservation and efficient management of Korean cattle breeds.