• Asian-Australasian Journal of Animal Sciences
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• v.5 no.3
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• pp.401-409
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• 1992

Genotype $\times$ environment (G $\times$ E) interactions must be understood if they are to be exploited to improve animal production, particularly in production systems associated with large environmental variations. The measurement and evaluation of G $\times$ E are discussed. Examples are presented that demonstrate G $\times$ E in different breeds of beef cattle for high temperatures, internal and external parasites and changes in quantity and quality of nutrition. It is demonstrated that productivity differences between genotypes or breeds under grazing conditions arise because of differences between genotypes in the combination of production potential and resistance to environmental stresses in relation to the levels of the relevant environmental stresses that are operating at the time. The $F_1$ cross between genotypes with high production potential (e.g. European Bos Taurus breeds) and those with high resistance to environmental stress (e.g. Asian and African Bos indicus and sanga breeds) is an exceptional genotype with a unique combination of these two sets of attributes. The principles for G $\times$ E developed for beef cattle are briefly discussed in relation to dairy cattle, pigs, poultry and buffalo.

• Korean Journal of Veterinary Service
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• v.32 no.2
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• pp.139-146
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• 2009

Bovine brucellosis is a zoonosis, long incubation period and chronic infectious disease, usually caused by Brucella abortus. This study was carried out to investigate the biotyping and biochemical characterization of B. abortus isolated from 208 farm 871 korean cattle and holstein diagnosed brucellosis by serological positive in Gyeongbuk province during the period from 2002 to 2006. B. abortus was isolated from 124 (14.2%) of 871 cattle, and isolated 110 (13.4%) of 820 Korean cattle and 14 (27.5%) of 51 holstein in breed. The uterus of korean cattle was isolated in 8 (17.8%) of 45 cattle and supramammary lymph none of holstein was isolated 11 (68.8%) of 16 cattle. 101 (12.5%) of 810 serological positive blood samples were isolated B. abortus. The isolation rate of B. abortus was correlated with antibody titers. The biochemical characterization of isolates was non-hemolytic, production of H$_2$S, oxidase-positive, catalase-positive, hydrolyzation of urea and growth of basic fuchsin dye medium. As a result, all of isolates was identified B. abortus bv 1. 124 isolates were susceptible to ampicillin, lincospectin, amikacin, gentamicin, kanamycin, neomycin, streptomycin, tetracycline, ciprofloxacin, norfloxacin and enrofloxacin.

• Korean Journal of Veterinary Service
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• v.42 no.2
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• pp.61-65
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• 2019

Seroprevalence of Coxiella burnetii (Q fever) in bulk-tank milk and cattle in Daegu area was analyzed from 2017 to 2018 by ELISA. The prevalence of antibodies in collected bulk-tank milk from 12 dairy cattle farms was 41.7% (10/24) and the seroprevalence of 249 cows reared in the area of Daegu was 3.2% (8/249) By age, the seroprevalence was 1.9% (2/105) in less than 2 years of age, 4.2% (5/119) between 3 and 5 years of age and 4.0% (1/25) in more than 6 years of age. By breed, the seroprevalence of the Hanwoo cattle was 1.2% (2/162) and the seroprevalence of the dairy cattle was 6.9% (6/87). The result suggested that seroprevalence of C. burnetii was relatively high in both bulk-tank milk samples and dairy cattle than the Hanwoo cattle. Based on these data, it is necessary to keep monitoring the prevalence of Q fever in Daegu area.

• Molecules and Cells
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• v.38 no.5
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• pp.466-473
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• 2015

Over the last 30 years, Hanwoo has been selectively bred to improve economically important traits. Hanwoo is currently the representative Korean native beef cattle breed, and it is believed that it shared an ancestor with a Chinese breed, Yanbian cattle, until the last century. However, these two breeds have experienced different selection pressures during recent decades. Here, we whole-genome sequenced 10 animals each of Hanwoo and Yanbian cattle (20 total) using the Illumina HiSeq 2000 sequencer. A total of approximately 3.12 and 3.07 billion sequence reads were mapped to the bovine reference sequence assembly (UMD 3.1) at an average of approximately 10.71- and 10.53-fold coverage for Hanwoo and Yanbian cattle, respectively. A total of 17,936,399 single nucleotide polymorphisms (SNPs) were yielded, of which 22.3% were found to be novel. By annotating the SNPs, we further retrieved numerous nonsynonymous SNPs that may be associated with traits of interest in cattle. Furthermore, we performed whole-genome screening to detect signatures of selection throughout the genome. We located several promising selective sweeps that are potentially responsible for economically important traits in cattle; the PPP1R12A gene is an example of a gene that potentially affects intramuscular fat content. These discoveries provide valuable genomic information regarding potential genomic markers that could predict traits of interest for breeding programs of these cattle breeds.

• Food Science of Animal Resources
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• v.24 no.4
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• pp.355-360
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• 2004

In Korean beef market, one of the major problems is mislabeling or fraudulent distribution of Holstein dairy meat or imported beef as domestic Hanwoo meat. Therefore, there has been a great need for a development of technology to identify beef breeds in meat and meat products. This study was carried out to develop the accurate and reliable method for the identification of beef breed using PCR-RFLP marker of MC1R, MGF and TYRPl genes affecting coat colors in cattle. A single base substitution (G\longrightarrowT transition) at the codon for amino acid position 104 of MC1R gene was identified between Hanwoo and Holstein and Angus breeds. The change at this position creates Msp I restriction site in Holstein and Angus, but not in Hanwoo. When the DNA amplified products (537 bp) was digested with Msp I, Hanwoo meat showed a single band of 537bp, while two fragments of 329bp and 208 bp were observed in Holstein meat and Angus breed, respectively. Thus, breed-specific RFLP marker in the MC1R gene can be used to distinguish between Hanwoo meat and Holstein and Angus meats. In the RFLP genotype of MGF gene, the frequency of r/r type was 75％ in Manwoo, whereas the frequency of R/R was 80％ in Hereford breed. Holstein and Angus breeds showed 100％ for R/r type. Therefore, Hanwoo meat showed significant difference in the MGF genotype frequencies compared with those of Holstein meat and imported beef cattle breeds. However, TYRP1 gene showed the same genotype in all breeds examined. Thus, this TYRP1 gene can not be used as a molecular marker for breed identification. As a consequence, we suggest that RFLP markers of the MC1R and MGF coat color genes could be used as DNA marker for identification of Hanwoo meat from Holstein and imported meats.

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.4
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• pp.461-464
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• 1996

The data on milk yield of 1837 healthy dairy cows of different genetic groups raised in Central Cattle Breeding and Dairy farms, Savar, Dhaka for the period from 1978 to 1992 were analyzed from first to 6th lactation. The mean (SE) milk yields of 100 days, 305 days, total lactation and total life time ($928{\pm}48.6kg$, $2,188{\pm}201.81kg$, $2,661{\pm}385.46kg$ and $11,134{\pm}2,916kg$) respectively, were in pure bred Friesian found to be highest among all genetic groups. The corresponding means in indigenous cows, $334{\pm}12.92kg$, $946{\pm}64.44kg$, $635{\pm}16.31kg$ and $3,934{\pm}402.21kg$ were the lowest. Among the $F_1$, $F_2$ and $F_3$ cross-breds, the $F_1$ half bred Friesian showed the highest performance in milk yield. The half bred Friesian in successive generations yielded low milk. Similar trend was obtained incase of cross-bred with other breeds such as Jersey, Sindhi and Sahiwal. From the over all results, it can be concluded that the performance in respect to milk yield of pure bred Friesian and their $F_1$ half bred with indigenous and Sahiwal were superior over the $F_2$ or $F_3$ cross-breds between Fresian and any other breed irrespective of proportion of blood. The performance in respect to production of indigenous local cow was found to be almost similar with that of Sahiwal breed.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.7
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• pp.933-950
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• 2018

In Japan, Wagyu cattle include four Japanese breeds; Black, Brown, Shorthorn, and Polled. Today, the renowned brand name Wagyu includes not only cattle produced in Japan, but also cattle produced in countries such as Australia and the United States. In recent years, the intramuscular fat percentage in beef (longissimus muscle) from Japanese Black cattle has increased to be greater than 30%. The Japanese Black breed is genetically predisposed to producing carcass lipids containing higher concentrations of monounsaturated fatty acids than other breeds. However, there are numerous problems with the management of this breed including high production costs, disposal of untreated excrement, the requirement for imported feed, and food security risks resulting from various viral diseases introduced by imported feed. The feeding system needs to shift to one that is more efficient, and improves management for farmers, food security for consumers, and the health environment for residents of Japan. Currently, we are developing a metabolic programming and an information and communications technology (ICT, or Interne of Things) management system for Wagyu beef production as future systems. If successful, we will produce safe, high-quality Wagyu beef using domestic pasture resources while solving the problems of how to utilize increasing areas of abandoned agricultural land and to make use of the plant-based feed resources in Japan's mountainous areas.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.5
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• pp.763-769
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• 2020

Objective: The experiment was conducted to study the effect of rambutan (Nephelium lappaceum) fruit peel powder (RP) on feed consumption, digestibility of nutrients, ruminal fermentation dynamics and microbial population in Thai breed cattle. Methods: Four, 2-year old (250±15 kg) beef bull crossbreds (75% Brahman×25% local breed) were allotted to experimental treatments using a 4×4 Latin square design. Four dietary supplementation treatments were imposed; non-supplementation (control, T1); supplementation of RP fed at 2% of dry matter intake (DMI) (low, T2); supplementation of RP fed at 4% of DMI (medium, T3) and supplementation of RP fed at 6% of DMI (high, T4). All cattle were given a concentrate supplement at 1% of body weight while Napier grass was provided as a free choice. Results: The findings revealed that RP supplementation did not negatively affect (p>0.05) DMI of Napier grass, while RP intake and total DMI were the greatest in the RP supplementation at 4% and 6% DMI. Nevertheless, the nutrients (dry matter, organic matter, crude protein, neutral detergent fiber, and acid detergent fiber) digestibilities were not changed in the RP supplementation groups. Rumen fermentation parameters especially those of total volatile fatty acids, acetate and butyrate were not significantly changed. However, the propionate concentration was remarkably increased (p<0.05) in the RP supplementation. Notably, the ratio of acetate to propionate, the number of protozoa, as well as the methane estimation were significantly reduced in the RP supplemented groups (4% and 6% of DMI), while the counts of bacteria was not altered. Conclusion: Supplementation of RP (4% of DMI) improved rumen propionate production, reduced protozoal population and methane estimation (p<0.05) without a negative effect on feed consumption and nutrients total tract digestibilities in beef cattle. Using dietary rambutan fruit peel powder has potential promise as a rumen regulator.

• Journal of Animal Science and Technology
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• v.54 no.6
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• pp.411-418
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• 2012

KIT gene is the major causative gene for coat color variation in diverse animal species. This gene regulates melanocyte migration from the neural crest to target tissues and the mutation of this gene can affect dominant white phenotypes in animals. Because this gene has a major influence for the coat color variation, single nucleotide polymorphisms (SNPs) in 14 Korean cattle (Hanwoo) and 5 Holstein individuals were investigated. The Hanwoo DNA samples included three different colored (5 Black, 5 Yellow and 4 Stripe) animals. Total 126 polymorphisms have been identified and 23 of them are located in the exon region. Also, 5 bp (TTCTC) and 3 bp (TCT) intronic indels in intron 3 and intron 5, respectively, were identified. Out of 23 exonic polymorphisms, 15 SNPs are the missense mutations and the rest of the SNPs are silence mutations. The neighbor-joining phylogenetic tree was constructed for the different colored animals using the obtained KIT gene sequences. Holstein breed showed a clear breed-specific cluster in the phylogenetic tree which is differed from Hanwoo. Also, three colored Hanwoo animals were not discriminated among the breeds. The KIT gene polymorphisms identified in this study will possibly give some solutions for the color variations in cattle with further verifications.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.4
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• pp.494-497
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• 2003

The study was carried out in Sahiwal, Holstein Friesian, Jersey and crossbred cattle and Murrah, Bhadwari, Jaffarabadi, Nagpuri and Surti buffaloes maintained at different organized herds to work out the polymorphism at growth hormone locus and study its effect on birth weight. A 223 bp fragment of the gene was amplified and digested with Alu I restriction enzyme. Two alleles, L and V with three genotypes LL, LV and VV were observed in Jersey, Holstein and cross bred cattle. Sahiwal cattle and buffalo were monomorphic for this locus producing only one genotype LL and one allele L. The frequency of L allele was comparatively higher in Holstein and crossbred cattle while in Jersey breed, the frequency of this allele was intermediate. The effect of genotype on birth weight was significant and LV genotype had higher birth weight than other genotypes. Hence, LV genotype in Holstein Friesian favored higher birth weight.