• Biotechnology and Bioprocess Engineering:BBE
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• 제9권5호
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• pp.389-392
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• 2004

In order to produce a harmful protein more efficiently, this expression cassette, dubbed pCol-MICT, is directed by the colicin promoter, and was constructed by the insertion of a $rrnBT_1T_2$ fragment of pEXP7, and a MxelnteinCBD fragment of pTXB3, into pSH375. To test whether harmful proteins, including proteolytic enzymes, could be effectively produced by this cassette, the carboxypeptidase (CPase) Taq gene was inserted into the pCol-MICT cassette to yield pCol-CPase Taq-MICT. E coli W3l 10 tells harboring pCol-CPase Taq-MICT produced a large quantity of this enzyme, as much as 47.2 mg of purified from per liter of culture, when cultured in the presence of mitomycin C ($0.4{\mu}g/mL$). This indicates that the colicin promoter-controlled E, coli expression cassette was able to produce almost 8 times of protein than the conventional tar promoter-based system, and that this cassette may be useful in the Synthesis of other harmful proteins.

• 미생물학회지
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• 제42권1호
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• pp.12-18
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• 2006

2003년부터 2004년까지 정북지역에서 발생한 설사환자로부터 17주, 돼지로부터 18주 등 총 35주의 Salmonella enterica serovar Typhimurium이 분리되었고, 분리된 균주를 대상으로 항균제 내성양상, class I integron 특성 및 pulse-field gel electrophoresis유형(PFGE 유형; pulsotype)이 조사되었다. 35주 모두가 한가지 이상의 항균제에 내성을 나타내었고, 돼지로부터 분리된 대부분의 균주는 ampicillin, chloramphenicol, streptomycin, sulfamethoxazole/trimethoprime, tetracyclin, nalidixic acid에 내성을 나타내었다. 35주를 대상으로 class I, II 및 III integron gene cassette를 검색한 결과, 설사환자로부터 분리된 17주 중 3주가 dhfrX-orfF-aadA2 integron gene cassette을 보유하였고, 돼지로부터 분리된 18주중 11주가 dhfrX-orfF-aadA2 integron gene cassette를, 1주가 aadA2 integron을 보유하였다. 그러나 35주 모두가 class 2 integron gene cassette와 class 3 integron gene cassette는 보유하지 않은 것으로 나타났다. 35주의 PEGE 유형은 5가지로 분류되었으며, 31주가 pulsotype A로 나머지 4주는 pulsotype B, C, D, E형으로 각각 나뉘어졌다. 이러한 결과로 볼 때 경북지역에서 사람과 돼지에서의 S. enterica serovar Typhimurium에 의한 살모넬라중은 몇 종류 유행주의 전파에 의한 것임을 보여줄다. dhfrX-orfF-aadA2 integron gene cassette를 보유한 13주가 pulsotype A, dhfrX-orfF-aadA2 integron gene cassette을 보유한 1주가 pulsotype B, aadA2 integron을 보유한 1주가 pulsotype E, integron을 보유하고 있지 않은 15주가 pulsotype A로 나타났다.

• Journal of Microbiology and Biotechnology
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• 제13권5호
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• pp.819-822
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• 2003

Polyketides are an extensive class of secondary metabolites with diverse molecular structures and biological activities. A plasmid-based minimal polyketide synthase (PKS) expression cassette was constructed using a subset of actinorhodin (act) biosynthetic genes (actI-orfl, actI-orf2, actI-orf3, actIII, actⅦ, and actIV) from Streptomyces coelicolor, which specify the construction of an orange-fluorescent anthraquinone product aloesaponarin II, a type II polyketide compound derived from one acetyl coenzyme A and 7 malonyl coenzyme A extender units. This system was designed as an indicator pathway in S. parvulus to generate a hybrid type II polyketide compound via gene-specific replacement. The act ${\beta}-ketoacyl$ synthase unit (actI-orfl and actI-orf2) in the expression cassette was specifically replaced with oxytetracycline ${\beta}-ketoacyl$ synthase otcY-orfl and otcY-orf2). This plasmid-based hybrid PKS cassette generated a novel orange-fluorescent compound structurally different from aloesaponarin II in both S. lividans and S. parvulus. In addition, several additional distinctive blue-fluorescent compounds were detected, when this hybrid PKS cassette was expressed in S. coelicolor B78 (actI-orf2 mutant), implying that the expression of plasmid-based hybrid PKS cassette in Streptomyces species should be an efficient way of generating hybrid type II polyketide compounds.

• 농업과학연구
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• 제31권1호
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• pp.35-44
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• 2004

In this study, the automatic system exchanging well-plates was developed as a basic stage of the genome project. The developed system consisted of the plate fixing well-plates, the well-plate cassette, the head to move a well-plate from the well-plate cassette to the plate fixing well-plates before genome work or from the plate to the cassette after the work, the manipulator to move the head on the X, Y and Z axes and the control system. The performance test to exchange well-plates with the robotic system developed was carried out. The time to set an well-plate from the well-plate cassette onto the board fixing well-plates was 55 seconds and the time for 9 ones was 8 minutes and 15 seconds. It took 57 seconds to move a well-plate from the board to the cassette and 8 minutes and 33 seconds for 9 ones.

• 대한설비공학회:학술대회논문집
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• 대한설비공학회 2009년도 하계학술발표대회 논문집
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• pp.1507-1512
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• 2009

Flow field in a roller conveyor system, induced from the movement of a cassette in which glasses for flat panel display are loaded, is numerically studied in this paper. Contamination on the glass surface by dust particles produced from mechanical friction between roller and cassette is predicted from the analysis results of flow fields. Results show that a large swirl flow is formed under the moving cassette with constant speed. This swirl flow is confined only under the cassette because two main streams from the backward and the fan filter unit on the top ceiling are sufficiently strong. Therefore, it can be said that possibility of the contamination by the particles originated from the friction is relatively low. It is also revealed that flow direction between glass plates is changed according to the speed of the cassette movement due to the shear force of glass plates.

• 대한전자공학회논문지
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• 제14권6호
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• pp.16-25
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• 1977

Microcomputer system과 음성 cassette tape recorder의 interface 방식에 관한 연구로서 병렬식 interface adapter를 사용하여 byte 조합을 software적으로 해결하였으며 간단한 motor제어도 할 수 있었다. 또 간단한 회로를 사용한데 비하여 byte당의 data처리 속도는 6.6ms가량 개선할 수 있었다.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2001년도 추계학술대회 논문집
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• pp.208-211
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• 2001

In the drawing from round billet to non-circular section there are two different processes through solid hole die(HD) and the other cassette roller dies(CRD). The CRD process has several cassette type rollers and a billet is able to move through the given gaps between two profiled rollers. The objective of this study is based on the analysis and evaluation of two aforementioned processes using experiments and finite element simulation. In order to simulate the multi-stage drawing process from circular sectioned billet to rounded square section, the finite element analysis is applied to the process using a commercially available DEFORM-3D code. Two types of experimental drawing tests through designed and manufactured dies for pure copper and aluminum alloy are carried out at room temperature. The analysis included comparison of material properties before and after drawing of each process and also provide some useful information by a FEM simulation.

• 대한디지털의료영상학회논문지
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• 제2권1호
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• pp.92-95
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• 1996

Computed Radiography(CR) is a relatively new technology that relies on an image plate(IP) as an alternate x-ray sensor to screen/film. Standard CR cassettes do not have lead foil behind the IP to control scatter radiation. The result of this study indicate that such control is needed. In most screen/film cassettes, that lines the rear of the cassette eliminates back scatter radiation. This study was performed to Investigate on the effects of back scatter in CR images by size of exposure field, distance between the CR cassette and the wall of radiography room. 1. It showed artifacts from hinges and clips located on the back of CR cassette by back scatter radiation. 2. The greater effects of back scatter radiation in CR images was attributed to the greater size of exposure field and the longer distance between the CR cassette and the wall of radiography room.

• 한국방사선학회논문지
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• 제11권2호
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• pp.117-122
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• 2017

본 연구는 IP 카세트를 이용하는 이동촬영 장비의 세균오염도를 조사하여 이동촬영으로 인한 병원 감염을 예방하고 감염교육의 기초자료를 제공하고자 실시하였다. 결과는 IP Cassette 1번에서 CNS, VRE가 검출되었다. 2번에서는 CNS, 3번에서는 CNS, Pseudomonas aeruginosa, 4번에서는 CNS, 5번에서는 CNS, Bacillus sp., 6번에서는 Enterococcus faecium이 검출되었다. 영상판독기에서는 Enterococcus faecium, Bacillus sp., 장비 이동 손잡이에서 Acinetobacter baumannii, 조작버튼에서는 Bacillus sp., 조사야 조절 손잡이에서는 CNS, Bacillus sp., X-선 발생 스위치에서는 Acinetobacter baumannii, 바코드기 에서는 CNS가 검출되었다. IP Cassette 이동테이블에서는 Bacillus sp. Acinetobacter baumannii, 납 앞치마에서는 CNS, Bacillus sp.가 검출되었다. 방사선사가 촬영 중 착용한 의료용 장갑에서는 Acinetobacter baumannii, CNS가 검출 되었다. 따라서 IP Cassette는 사용 후에는 반드시 소독하고 재사용하며, 영상판독기, IP 이동테이블은 오염된 IP Cassette를 통해 세균이 전파될 수 있으므로 사용 후에는 소독을 실시해야 한다. 환자와 직접 접촉하는 의료용 장갑도 1회 사용 후 교체하고 촬영 시 접촉이 많은 X-발생 스위치, 납 앞치마도 철저히 소독하여 이동촬영으로 인한 감염을 예방하여야 한다.

• 한국막학회:학술대회논문집
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• 한국막학회 1992년도 춘계 총회 및 학술발표회
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• pp.9-10
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• 1992

The Cassette system is a newly developed member of the range of Membrane Filtration Systems, designed for the ultrafiltration or microfiltraion of large volumes of fluid with main application emphasis being on water filtration.