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Structural Analysis of Class I Integron Gene Cassette and Assessment of Genetic Relationships by PFGE of Salmonella enterica Serovar Typhimurium Isolated in Gyeongbuk Area  

Sohn, Chang-Kyu (biology Section, Gyeongsangbuk-Do Health and Environment Institute)
Lee, Jung-A (Gyeongbuk Veterinary Service Laboratory)
Lee, Do-Young (biology Section, Gyeongsangbuk-Do Health and Environment Institute)
Hun, Wan (biology Section, Gyeongsangbuk-Do Health and Environment Institute)
Jung, Jung-Kyo (biology Section, Gyeongsangbuk-Do Health and Environment Institute)
Publication Information
Korean Journal of Microbiology / v.42, no.1, 2006 , pp. 12-18 More about this Journal
Abstract
Thirty five Salmonella enterica serovar Typhimurium strains were isolated from diarrheic patients and pigs in Gyeongbuk area from 2003 to 2004. All 35 strains (17 strains from diarrheic patients and 18 from pigs) were resistant to more than one drug and most of strains isolated from pigs were resistant to ampicillin, ohloram-phenicol, streptomycin, sulfamethoxazole-trimethoprime, tetracyclin and nalidixic acid. Each isolate was also screened or the presence of class I, II and III integron gene cassettes. Among 35 strains,3 out of 17 strains isolated from diarrheic patients, carried dhfrX-orfF-aadA2 integron gene cassette and among 18 strains isolated from diseased pigs, 11 strains carried dhfrX-orfF-aadA2 integron gene cassette and 1 strain carried aadA2 integron only. But any class II and class II integron gene cassette were not detected in 35 strains. Thirty five strains were divided by five pulsotypes. Thirty one strains out of thirty five were pulsotype A. Among the remaining 4 strains, one each strain belonged to pulsotype B, C, D and pulsotype E. This data of pulsotypes showed that the widespread of pulsotype A, Salmonella enterica serovar Typhimurium in human and pigs in Gyeongbuk area may have been caused by the dissemination of a few epidemic strains in this area. Thirteen strains contain dhfrX-orfF-aadA2 integron gene cassette showed pulsotype A and one strain contains dhfrX-orfF-aadA2 integron gene cassette showed pulsotype B. One strain contains aadA2 integron showed pulsotype E. But fifteen strains do not contain any integron showed pulsotype A.
Keywords
antibiotic resistance pattern; class I integron; pfge pattern (pulsotype); Salmonella enterica Serovar Typhimurium;
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1 White, D.G., Zhao, S., MaDermott, P.F., Ayers, S., Friedman, S., Sherwood, J., Breider-Foley, M., and Nolan, L.K. 2003. Characterization of integron mediated antimicrobial resistance in Salmonella isolated from diseased swine. The Canadine J. of Vet. Res. 67, 39-47
2 Adrine, P.V., C.J. Thomson, K.P. Klugman, and S.G.B. Amyes. 2000. New gene cassette for trimethoprim resistance, dfr13, and streptomycin-spectinomycin resistance, aadA4, inserted on a class I integron. Antimicrobe. Agent Chemother. 44, 355-361   DOI
3 Peters, E.D., M.A. Leverstein, A.T.A. Box, J. Verhoef, and A.C. Fluit. 2001. Novel gene cassette and integron. Antimicrobe. Agent Chemother. 45, 2961-2964   DOI   ScienceOn
4 Kazama, H., K. Kizu, M. Iwasaki, H. Hamashima, M. Sasatsu, and T. Arai. 1995. Isolation of a new integron that include a streptomycin resistance gene from R plasmid of Pseudomonas aeroginsa. FEMS Microbiol. Lett. 134, 137-141   DOI
5 Lavesque, C., L. Pyche, C. Larose, and P.H. Roy. 1995. PCR mapping of integron gen reveal several novel combinations of resistance genes. Antimicrobe. Agent Chemother. 39, 185-191   DOI   ScienceOn
6 Sambrook, J.E., F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd, ed. Cold Spring Harbor Laboratory, New York
7 Collis, C.M., and R.M. Hall. 1992. Site specific deletion and rearrangement of integron inserted genes catalyzed by the integron DNA integrase. J. Bacteriol. 174, 1574-1585   DOI
8 Kotler, L., Sobolev, I., and Ulanovsky, L. 1994. DNA sequencing: modular primers for automated walking. Biotechniques 17, 554-559
9 Cruchaga, S., Echeita A., Aladuena A., Garcina-Pena J., Frias N., and Usera M.A. in 1998. Antimicrobial resistance in Salmonella from humen, food and animals in Spain J. Antimicro. Chemother. 47, 315-321
10 H.S. Yu, , J.C. Lee, H.Y. Kang, D.W. Ro, J.Y. Chung, Y.S. Jeong, S.H. Tae, C.H. Choi, E.Y. Lee, S.Y. Seol, Y.C. Lee, and D.T. Cho. 2003. Change in gene cassettes of class I integron among Escherichia coli isolates from urine specimen collected in Korea during the last two decades. J. Clin. Microbiol. 41, 5429-5433   DOI
11 J.Y. Oh, H.S. Yu, S.K. Kim, S.Y. Seol, D.T. Cho, and J.C. Lee. 2003. Changes in patterns of antimicrobial susceptibility and integron carriage among Shigella sonnei isolated from southwestern Korea during epidemic period. J. Clin. Microbiol. 41, 421-423   DOI
12 Moss, P.J., and M.W. McKendrick. 1997. Bacterial gastroenteritis. Curr. Opin. Infect. Dis. 10, 402-407   DOI
13 Davies, J. 1994. Inactivation of antibiotic and the dissemination of resistance gene. Science 264, 375-382   DOI
14 Borch E., Nesbakken T., and Christensen S. 1996. Hazard identification in swine slaughter with respect to foodborne bacteria. Int. J. Food Microbiol. 30, 9-25   DOI   ScienceOn
15 Altschul, S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller, and D.J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search program. Nucleic Acid Res. 25, 3389-3402   DOI
16 Fluit, A.C., and F.J. Schmitz. 1982. ClassI integron gene cassettes, mobility, and epidemiology. Eur. J. Clin. Microbiol. Infect. Dis. 18, 761-770   DOI
17 Hall, R.M. 1997. Mobile gene cassette and integrons: moving antibiotic resistance genes in gram-negative bacteria. Ciba Found symp. 207, 192-202
18 Bito, A., and M. Susani. 1994. Revised analysis of aadA2 gene of plasmid PSa. Antimicrobe. Agent Chemother. 38, 1172-1175   DOI   ScienceOn
19 Levesque, C., L. Pyche, C. Larose, and P.H. Roy. 1995. PCR mapping of integron reveal several novel combinations of resistance genes. Antimicrobe. Agent Chemother. 39, 185-191   DOI   ScienceOn
20 Hall, R.M., and C.M. Collis. 1995. Mobile gene cassette and integrons: capture and spread of genes by site specific recombination. Mol. Microbiol. 15, 593-600   DOI   ScienceOn
21 Guerra, B., S. Soto, S. Cal, and M. C. Mendoza. 2000. Antimicrobial resistance and spread of class I integron among Salmonella serotypes. Antimicrobe. Agent Chemother. 44, 2166-2169   DOI
22 Martinez-Freijo, P., A.C. Fluit, F.J. Schmitz, J. Verhoev, and M.E. Jones. 1999. Many class. Xintegrons comprise stable structure occurring in different species of Enterobacteriaecae isolated from wide spread geographic regions in Europe. Antimicrobe. Agent Chemother. 43, 686-689
23 Gautom, R. 1997. Rapid PFGE protocol for typing of E. coli O157:H7 and other gram negative organism in one day. J. Clin.Microbiol. 35, 2977-2980
24 Jung B. Y., Park K. M., and Kim B. H. 2004. Antimicrobial susceptibility patterns of Salmonella Typhimurium isolated from diseased pigs. Kor. J. Vet. Pub. Hlth. 28(2), 109-211
25 Ewing, W.H. 1986. Identification of Enterobacteriaceae, Elsevier, 4th ed. New york, p.108-318
26 National Committee Clinical Laboratory Standards. 1997. Performance standards for antimicrobial disk susceptibility tests, 6th ed. Approved Standard NCCLS document M2-A6, National Committee for Clinical Laboratory Standards, Villanova, Pa. USA
27 Tosini, F., P. Visca, I. Luzzi, A.M. Dionisi, C. Pezzella, A. Petrucca, and A. carattoli. 2000. class I integron -borne multiple-antibiotic resistance and incL/M plasmid in Salmonella enterica serotype Typhimurium. Antimicrobe. Agent Chemother. 42, 3053-3058
28 Recchia, G.D., and R.M. Hall. 1995. Gene cassette: a new class of mobile element. Microbiology 141, 3015-3027   DOI   ScienceOn
29 Tenover, F.C., R.D. Arbeit, R.V. Goering, P.A. Mickelsen, B.E. Murray, D.H. Persing, and B. Swaminathan. 1995. Interpreting chromosomal DNA restriction patterns produced by pulse-field gel electrophoresis: criteria for bacteria strain typing. J. Clin.Microbiol. 33, 2233-2239
30 Law, J.C., M. Angus, G. Hopkin, D. Munro, and S.C. Rankin. 1997. Antimicrobial resistance of Salmonella enterica serovar Typhimurium DT104 isolates and investigation of strain with transferable apramycin resistance. Epidemiol. Infect. 118, 97-103   DOI
31 Hampton, M.D., E.J. Threlfall, J.A. Frost, L.R. Ward, and B. Rowe. 1995. Salmonella DT193: Differentiation of an epidemic phage type by antibiogram, plasmid profile, plasmid finger print and Salmonella plasmid virulence(spv) gene probe. J. Appl. Bacteriol. 78, 402-408   DOI