Development of an E. coli Expression Cassette for the Efficient Production of a Harmful Protein |
Kim Ok Soo
(Major in Bioscience and Biotechnology, Division of Environmental Engineering and Biotechnology, Silla University)
Kwak Hwan Jong (Department of Pathology, College of Medicine, The Catholic University of Korea) Lee Jae-Hwa (Major in Bioscience and Biotechnology, Division of Environmental Engineering and Biotechnology, Silla University) Ha Jong Myung (Major in Bioscience and Biotechnology, Division of Environmental Engineering and Biotechnology, Silla University) Ha Bae-Jin (Major in Bioscience and Biotechnology, Division of Environmental Engineering and Biotechnology, Silla University) Lee Sang-Hyeon (Major in Bioscience and Biotechnology, Division of Environmental Engineering and Biotechnology, Silla University) |
1 | Hong, J.-K., M.-S. Park, F M. Raushel, and Y-H. Khang (2003) Expression and characterization of Escherichia coli prolidase with organophosphorus compounds. Biotechnol. Bioprocess Eng. 8: 126-129 |
2 | Lee, S.-H., H. Taguchi, E. Yoshimura, E. Minagawa, S. Kaminogawa, T. Ohta, and H. Matsuzawa (1994) Carboxypeptidase Taq, a thermostable zinc enzyme, from Therrnus aquaticus YT-1: Molecular cloning, sequencing, and expression of the encoding gene in Escherichia coli. Biosci. Biotech. Biochem. 58: 1490-1495 DOI ScienceOn |
3 | Ebina, Y., Y. Takahara, K. Shirabe, M. Yamada, T. Nakazawa, and A. Nakazawa (1983) Plasmid-encoded regulation of colicin El gene expression. J. Bacteriol. 156: 487-492 |
4 | Parker, R. C. (1986) Mitomycin C-induced bidirectional transcription from the colicin E1 promoter region in plasmid ColE1. Biochirn. Biophys. Acta 868: 39-44 DOI ScienceOn |
5 | Petit, M. A, W. Bedale, J. Osipiuk, C. Lu, M. Rajagopalan, P. McInerney, M. F Goodman, H. Echols (1994) Sequential folding of UmuC by the Hsp70 and Hsp60 chaperone complexes of Escherichia coli. J. Bioi. Chem. 269: 23824-23829 |
6 | Sanger, F., S. Nicklen, and A. R. Coulson (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74: 5463-5467 DOI ScienceOn |
7 | Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685 DOI PUBMED ScienceOn |
8 | Masaki, H., A Akutsu, T. Uozumi, and T. Ohta (1991) Identification of a unique specificity determinant of the colicin E3 immunity protein. Gene 107: 133-138 DOI PUBMED ScienceOn |
9 | Masaki, H. and T. Ohta (1985) Colicin E3 and its immunity genes. J. Mol. Biol. 182: 217-227 DOI PUBMED |
10 | Rosen, H. (1957) A modified ninhydrin colorimetric analysis for amino acids. Arch. Biochem. Biophys. 67: 10-15 DOI PUBMED ScienceOn |
11 | Van den Elzen, P. J., M. J. Maat, H. H. B., Walters, E. Veltkamp, and H. J. Nijkamp (1982) The nucleotide sequence of the bacteriocin promoters of plasmids CIo DF13 and Col E1: Role of lexA repressor and cAmp in the regulation of promoter activity. Nucleic Acids Res. 10: 1913-1928 DOI ScienceOn |
12 | Waleh, N. S., I. Sohel, J. B. Lazar, D. V. Hudson, P. Sze, P. A. Underhill, and P. H. Johnson (1992) Expression of synthetic genes encoding fused proteins under tight control of modified regulatory regions of the colicin operon. Gene 117: 7-14 DOI ScienceOn |
13 | Cavard, D., S. P. Howard, R. Lloubes, and C. Lazdunski (1989) High-level expression of the colicin A lysis protein. Mol. Gen. Genet. 217: 511-519 DOI PUBMED |
14 | Waleh, N. S. and P. H. Johnson (1985) Structural and functional organization of the colicin E1 operon. Proc. Natl. Acad. Sci. USA 82: 8389-8393 DOI ScienceOn |