• Journal of Periodontal and Implant Science
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• v.49 no.3
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• pp.138-147
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• 2019

Purpose: Several studies have shown that the oral cavity is a secondary location for Helicobacter pylori colonization and that H. pylori is associated with the severity of periodontitis. This study investigated whether H. pylori had an effect on the periodontium. We established an invasion model of a standard strain of H. pylori in human periodontal ligament fibroblasts (hPDLFs), and evaluated the effects of H. pylori on cell proliferation and cell cycle progression. Methods: Different concentrations of H. pylori were used to infect hPDLFs, with 6 hours of co-culture. The multiplicity of infection in the low- and high-concentration groups was 10:1 and 100:1, respectively. The Cell Counting Kit-8 method and Ki-67 immunofluorescence were used to detect cell proliferation. Flow cytometry, quantitative real-time polymerase chain reaction, and western blots were used to detect cell cycle progression. In the high-concentration group, the invasion of H. pylori was observed by transmission electron microscopy. Results: It was found that H. pylori invaded the fibroblasts, with cytoplasmic localization. Analyses of cell proliferation and flow cytometry showed that H. pylori inhibited the proliferation of periodontal fibroblasts by causing G2 phase arrest. The inhibition of proliferation and G2 phase arrest were more obvious in the high-concentration group. In the low-concentration group, the G2 phase regulatory factors cyclin dependent kinase 1 (CDK1) and cell division cycle 25C (Cdc25C) were upregulated, while cyclin B1 was inhibited. However, in the high-concentration group, cyclin B1 was upregulated and CDK1 was inhibited. Furthermore, the deactivated states of tyrosine phosphorylation of CDK1 (CDK1-Y15) and serine phosphorylation of Cdc25C (Cdc25C-S216) were upregulated after H. pylori infection. Conclusions: In our model, H. pylori inhibited the proliferation of hPDLFs and exerted an invasive effect, causing G2 phase arrest via the Cdc25C/CDK1/cyclin B1 signaling cascade. Its inhibitory effect on proliferation was stronger in the high-concentration group.

• Journal of Adhesion and Interface
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• v.16 no.1
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• pp.15-21
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• 2015

In this study, rheological properties and flow dynamics in roll coating process of basecoat paints have been investigated for automotive precoated metal (PCM) sheet applications. Various rheological properties for basecoats with three colors (black, blue, and silver), such as shear viscosity data at room temperature and elastic/viscous moduli under thermal curing condition, have been measured using a rotational rheometer. It is found that the relative portion of function groups inside basecoats and their viscosity level have greatly affected the formation of crosslinked networks by thermal curing. Also, operability coating windows for basecoats have been established in three-roll coating process system by observing their flow instabilities such as ribbing and cascade. It is confirmed that rheological approaches applied in this study have been usefully applied to develop environmentally-friendly PCM coating technology and optimally control the coating operations for non-Newtonian PCM paints.

• The KSFM Journal of Fluid Machinery
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• v.16 no.6
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• pp.19-25
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• 2013

The effect of tip gap height on heat/mass transfer characteristics on the floor of cavity squealer tip has been investigated in a turbine cascade for power generation by employing the naphthalene sublimation technique. The squealer rim height is chosen to be an optimal one of $h_{st}/c$ = 5.51% for the tip gap height-to-chord ratios of h/c = 1.0, 2.0, 3.0 and 4.0%. The results show that heat transfer on the cavity floor is strongly dependent upon the behavior of the cavity flow falling down onto the floor. For lower h/c, the floor heat transfer is influenced by the tip leakage flow falling down along the inner face of the suction-side squealer, whereas the floor heat transfer for higher h/c is augmented mainly due to the impingement of leakage flow on the floor near the leading edge. Compared to the plane tip surface heat transfer, the cavity floor heat transfer is less influenced by h/c. For h/c = 1.0%, the average thermal load is as low as a half of the plane tip surface one, and the difference in the thermal load between the two cases tends to decrease with increasing h/c.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.25 no.3
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• pp.414-421
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• 2001

The present study is conducted to investigate the local heat/mass transfer characteristics on the shroud with blade tip clearances. The relative motion between blade and shroud has little influence on the overall heat transfer characteristics, except some local effects. Therefore, the relative motion between the blade and shroud is neglected in this study. A naphthalene sublimation method is employed to determine the detailed local heat/mass transfer coefficients on the surface of the shroud. The tip clearance is changed from 0.66% to 2.85% of the blade chord length. The flow enters the gap between the blade tip and shroud at the pressure side due to the pressure difference. Therefore, the heat/mass transfer characteristics on the shroud are changed significantly from those with endwall. At first, high heat/mass transfer occurs along the profile of blade at the pressure side due to the entrance effect and acceleration of the gap flow. Then, the heat/mass transfer coefficients on the shroud increase along the suction side of the blade because tip leakage vortices are generated and interact with the main flow. The results show that the heat/mass transfer characteristics are changed largely with the gap distance between the tip of turbine blade and the shroud.

• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.231-231
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• 2015

Rapid development in the upper reaches of the Mekong River, in the form of construction of large hydropower dams and reservoirs, large irrigation schemes, and rapid urban development, is putting water resources under stress. Many scientific reports have pointed out that cascade dams along the Mekong River lead to serious problems: not only hydrologically but also a decline of agricultural productivity due to a decrease of sediment supply in the Mekong Delta and a change of fish amount due to drastic change of the water environment. Cambodia and Vietnam, located in the lowest Mekong basin, are gravely affected by radical changes of hydrologic regime due to Mekong River developments. In particular, the Tonle Sap Lake in Cambodia is very sensitive to the flood cycle and flow variation of the Mekong River as well as inflow water quality from the Mekong River. More than 50% of Cambodian GDP depends on the primary industries such as agriculture, fishing, and forestry, and the Tonle Sap Lake plays an important role to support the national economy in Cambodia. In addition, Cambodian people usually take nourishment from the fish of Tonle Sap Lake. This research aims to assess the impacts of the Mekong river flow alternation on the hydrologic regime of the Mekong River - Tonle Sap Lake. We carried out rainfall-runoff-inundation simulation using CAESER-LISFLOOD for integrated water resource management in the Tonle Sap Basin and then analyze flood inundation variation of the Tonle Sap Lake due to the scenarios. Furthermore, the simulated inundation maps were compared to MODIS satellite images for model verification and hydrologic prediction.

• Nuclear Engineering and Technology
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• v.52 no.9
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• pp.1945-1954
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• 2020

Subchannel code is one of the effective simulation tools for thermal-hydraulic analysis in nuclear reactor core. In order to reduce the computational cost and improve the calculation efficiency, empirical correlation of turbulent mixing coefficient is employed to calculate the lateral mixing velocity between adjacent subchannels. However, correlations utilized currently are often fitted from data achieved in central channel of fuel assembly, which would simply neglect the wall effects. In this paper, the CFD approach based on spectral element method is employed to predict turbulent mixing phenomena through gaps in 3 × 3 bare tight lattice rod bundle and investigate the flow pulsation through gaps in different positions. Re = 5000,10000,20500 and P/D = 1.03 and 1.06 have been covered in the simulation cases. With a well verified mesh, lateral velocities at gap center between corner channel and wall channel (W-Co), wall channel and wall channel (W-W), wall channel and center channel (W-C) as well as center channel and center channel (C-C) are collected and compared with each other. The obvious turbulent mixing distributions are presented in the different channels of rod bundle. The peak frequency values at W-Co channel could have about 40%-50% reduction comparing with the C-C channel value and the turbulent mixing coefficient β could decrease around 25%. corrections for β should be performed in subchannel code at wall channel and corner channel for a reasonable prediction result. A preliminary analysis on fluctuation at channel gap has also performed. Eddy cascade should be considered carefully in detailed analysis for fluctuating in rod bundle.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.30 no.11 s.254
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• pp.1131-1138
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• 2006

Aerodynamic shape design of a partial admission turbine using CFD has been performed. Two step approaches are adopted in this study. Firstly, two-dimensional blade shape is optimized using CFD and genetic algorithm. Initially, the turbine cascade shape is represented by four design parameters. By controlling the design parameters as variables, the non-gradient search is analyzed for obtaining the maximum efficiency. The final two-dimensional blade proved to have a more blade power than the initial blade. Secondly, the three-dimensional CFD analysis including the nozzle, rotor and stator has been conducted. To avoid a heavy computational load due to an unsteady calculation, the frozen rotor method is implemented in steady calculation. The frozen rotor method can detect a variation of the flow-field dependent upon the blade's circumferential position relative to the nozzle. It gives a better idea of wake loss mechanism starting from the lip of the nozzle than the mixing plane concept. Finally, the combination of two and three dimensional design method of the partial admission turbine in this study has proven to be a robust tool in development phase.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.18 no.5
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• pp.401-406
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• 2005

Two dimensional MEDICI simulator is used to study the electrical characteristics of Dual Gate Emitter Switched Thyristor. The simulation is done in terms of the current-voltage characteristics with the variations of p-base impurity concentrations and current flow. Compared with the other power devices such as MOS Controlled Cascade Thyristor(MCCT), Conventional Emitter Switched Thyristor(C-EST) and Dual Channel Emitter Switched Thyristor(DC-EST), Dual Gate Emitter Switched Thyristor(DG-EST) shows to have tile better electrical characteristics, which is the high latch-up current density and low forward voltage-drop. The proposed DG-EST which has a non-planer u-base structure under the floating N+ emitter indicates to have the better characteristics of latch-up current and breakover voltage in spite of the same turn-off characteristics.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.7 no.4
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• pp.566-576
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• 1995

A Computational code has been developed in order to design axial fans by the numerical optimization techniques incorporated with flow analysis code solving three-dimensional Navier-Stokes equation. The steepest descent method and the conjugate gradient method are used to look for the search direction in the design space, and the golden section method is used for one-dimensional search. To solve the constrained optimization problem, sequential unconstrained minimization technique, SUMT, is used with imposed quadratic extended interior penalty functions. In the optimization of two-dimensional cascade design, the ratio of drag coefficient to lift coefficient is minimized by the design variables such as maximum thickness, maximum ordinate of camber and chord wise position of maximum ordinate. In the application of this numerical optimization technique to the design of an axial fan, the efficiency is maximized by the design variables related to the sweep angle distributed by quadratic function along the hub to tip of fan.

• The Korean Journal of Emergency Medical Services
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• v.25 no.1
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• pp.243-249
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• 2021

A decrease in coronary blood flow leads to an imbalance between the supply of oxygen to the myocardium and its demand, and reversible or irreversible damage to the myocardium could occur depending on the severity of the resultant ischemia and the duration of the imbalance. This imbalance results in a cascade of ischemic reactions in the following order: metabolic abnormalities, diastolic dysfunction, systolic dysfunction, and electrocardiogram changes. Variant angina is caused by the closure of the coronary artery due to reversible coronary artery spasm, resulting in myocardial ischemia and subsequent chest pain as a clinical symptom. Variant angina may be observed as ST segment elevation in electrocardiogram measured when present in chest pain. However, 12-lead electrocardiogram performed after the patient's chest pain resolves does not help in the diagnosis. Since the duration of chest pain appears to be <15 minutes, it is important to perform the 12-lead electrocardiogram when clinical symptoms are present. If nitroglycerin is administered without performing 12-lead electrocardiogram by 119 pre-hospital paramedics, the chest pain would be resolved, making it impossible to identify changes in the ST segment. Before administration of nitroglycerin, changes in the ST segment must be recorded by performing 12-lead electrocardiogram.