• Biomedical Science Letters
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• v.29 no.4
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• pp.231-241
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• 2023

Messenger RNA (mRNA)-based vaccines and treatments have recently emerged as a promising strategy. Naked mRNA presents various limitations for direct delivery. Therefore, in this paper, Lipid Nanoparticles (LNPs) were utilized for the delivery of mRNA. Lipid nanoparticle (LNP) mRNA systems are highly effective as vaccines, but their efficacy for pulmonary delivery has not yet been fully established. Additionally, research on effective delivery systems and administration methods for vaccines is required to resolve the stability and degradation issues associated with naked mRNA delivery. This study aimed to determine mRNA delivery efficiency via the inhalation of a lipid nanoparticle (LNP) formulation designed specifically for pulmonary delivery. To this purpose, we built a library of seven LNP configurations with different lipid molar and N/P ratios and evaluated their encapsulation efficiency using gel retardation assay. Among the tested LNPs, LNP1, LNP2-2, and LNP3-2 demonstrated high transfection efficiency in vitro based on FACS analyses luciferase assays, and intracellular accumulation tests. The mRNA delivery efficiencies of the selected LNPs after inhalation and intravenous injection were compared and evaluated. LNP2-2 showed the highest mRNA expression in healthy mouse lungs when aerosolized and was found to be non-toxic. These results indicate that LNP2-2 is a promising carrier for lung mRNA delivery via inhalation.

• Strategy21
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• s.41
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• pp.52-84
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• 2017

After finishing Cold War, the U.S. Navy's ability to Sea control has been gradually eroded last 15-20 years. The global security environment demands that the surface Navy rededicate itself to sea control, as a new group of potential adversaries is working to deny U.S. navy command of the sea. China has been increasing their sea denial capability, such as extended anti-surface cruise missile and anti-surface ballistic missile. To cope with this situation, the U.S. Naval Surface Forces Command has announced Surface Forces Strategy: Return to Sea Control. It is a new operating and organizing concept for the U.S. surface fleet called 'distributed lethality'. Under distributed lethality, offensive weapons such as new ASCMs are to be distributed more widely across all types of Navy surface ships, and new operational concept for Navy surface fleet's capability for attacking enemy ships and make it less possible for an enemy to cripple the U.S. fleet by concentrating its attack on a few very high-value Navy surface ships. By increasing the lethality of the surface ships and distributing them across wide areas, the Navy forces potential adversaries to not only consider the threat from our carrier-based aircraft and submarines, but they now consider the threat form all of those surface ships. This idea of using the distributed lethality template to generate surface action groups and adaptive force package and to start thinking about to increase the lethal efficacy of these ships. The U.S. Navy believes distributed lethality increases the Navy's sea control capability and expands U.S. conventional deterrence. Funding new weapons and renovated operating concept to field a more lethal and distributed force will enable us to establish sea control, even in contested area. The U.S. Navy's Surface Forces Strategy provides some useful implications for The ROK Navy. First the ROK Navy need to reconsider sea control mission. securing sea control and exploiting sea control are in a close connection. However, recently the ROK Navy only focuses on exploiting sea control, for instance land attack mission. the ROK Navy is required to reinvigorate sea control mission, such as anti-surface warfare and anti-air warfare. Second, the ROK Navy must seek the way to improve its warfighting capability. It can be achieved by developing high-edge weapons and designing renewed operating concept and embraced new weapon's extended capabilities.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.3B
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• pp.239-253
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• 2006

For guaranteeing the international competitive power of SW industry that is the core industry for the future, the training of SW technical manpower as an architect is needed. They must confront the change of worldwide technology and also lead the design and analysis of SW industry for the future. However, SW technical manpower based on regular career tends to change his employment as a general manager in current status. For solve this situation and reconsider the quality level of SW technical manpower, we need to propose the systematic and effective growth direction for them. Therefore, we propose the systematic and effective growth direction for domestic SW technical manpower and a plan to be realized by suggesting the carrier path of high level SW analysis/design manpower in this paper.

• Journal of Communications and Networks
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• v.14 no.5
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• pp.578-587
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• 2012

One of the key elements in the emerging, packet-based long term evolution (LTE) cellular systems is the deployment of multiple femtocells for the improvement of coverage and data rate. However, arbitrary overlaps in the coverage of these femtocells make the handover operation more complex and challenging. As the existing handover strategy of LTE systems considers only carrier to interference plus noise ratio (CINR), it often suffers from resource constraints in the target femtocell, thereby leading to handover failure. In this paper, we propose a new efficient, multi-objective handover solution for LTE cellular systems. The proposed solution considers multiple parameters like signal strength and available bandwidth in the selection of the optimal target cell. This results in a significant increase in the handover success rate, thereby reducing the blocking of handover and new sessions. The overall handover process is modeled and analyzed by a three-dimensional Markov chain. The analytical results for the major performance metrics closely resemble the simulation results. The simulation results show that the proposed multi-objective handover offers considerable improvement in the session blocking rates, session queuing delay, handover latency, and goodput during handover.

• Journal of Power Electronics
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• v.15 no.4
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• pp.939-950
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• 2015

A single-stage AC-AC converter has been designed for a wind energy conversion system (WECS) that eliminates multistage operation and DC-link filter elements, thus resolving size, weight, and reliability issues. A simple switching strategy is used to control the switches that changes the variable-frequency AC output of an electrical generator to a constant-frequency supply to feed into a distributed electrical load/grid. In addition, a modified random sinusoidal pulse width modulation (RSPWM) technique has been developed for the designed converter to make the overall system more efficient by increasing generating power capacity and reducing the effects of inter-harmonics and sub-harmonics generated in the WECS. The technique uses carrier and reference waves of variable switching frequency to calculate the firing angles of the switches of the converter so that the three-phase output voltage of the converter is very close to a sine wave with reduced THD. A comparison of the performance of the proposed RSPWM technique with the conventional SPWM demonstrated that the power generated by a turbine in the proposed approximately increased by 5% to 10% and THD reduces by 40% both in voltage and current with respect to conventional SPWM.

• The Transactions of the Korean Institute of Power Electronics
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• v.16 no.3
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• pp.283-292
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• 2011

This paper proposed a new multi-level inverter topology based on a H-bridge with two switches and two diodes connected to the DC-link. The output voltage of the proposed topology is quite closer to a sinusoidal waveform compared with a typical single phase inverter. The proposed multi-level inverter is applicable to a power conditioning system for renewable energy sources, and it can be also used as a building block of a cascaded multi-level inverter for a high voltage application. In case of conventional H-bridge type or NPC type multi-level inverter, 8 controllable switches are used to obtain a 5 level output voltage, but the proposed multi-level inverter requires only 6 controllable switches. Thus the circuit configuration is quite simple, reliable and cost-effective implementation is possible. The efficiency can be improved owing to the reduction of the switching loss. A new PWM method based on POD modulation is suggested which requires only one carrier signal. The switching sequence to make the capacitor voltage balanced is also considered. The feasibility is studied through simulation and experiment.

• Journal of Ginseng Research
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• v.47 no.2
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• pp.291-301
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• 2023

Introduction: Non-small cell lung cancer (NSCLC) patients are particularly vulnerable to the Coronavirus Disease-2019 (COVID-19). Currently, no anti-NSCLC/COVID-19 treatment options are available. As ginsenoside Rg3 is beneficial to NSCLC patients and has been identified as an entry inhibitor of the virus, this study aims to explore underlying pharmacological mechanisms of ginsenoside Rg3 for the treatment of NSCLC patients with COVID-19. Methods: Based on a large-scale data mining and systemic biological analysis, this study investigated target genes, biological processes, pharmacological mechanisms, and underlying immune implications of ginsenoside Rg3 for NSCLC patients with COVID-19. Results: An important gene set containing 26 target genes was built. Target genes with significant prognostic value were identified, including baculoviral IAP repeat containing 5 (BIRC5), carbonic anhydrase 9 (CA9), endothelin receptor type B (EDNRB), glucagon receptor (GCGR), interleukin 2 (IL2), peptidyl arginine deiminase 4 (PADI4), and solute carrier organic anion transporter family member 1B1 (SLCO1B1). The expression of target genes was significantly correlated with the infiltration level of macrophages, eosinophils, natural killer cells, and T lymphocytes. Ginsenoside Rg3 may benefit NSCLC patients with COVID-19 by regulating signaling pathways primarily involved in anti-inflammation, immunomodulation, cell cycle, cell fate, carcinogenesis, and hemodynamics. Conclusions: This study provided a comprehensive strategy for drug discovery in NSCLC and COVID-19 based on systemic biology approaches. Ginsenoside Rg3 may be a prospective drug for NSCLC patients with COVID-19. Future studies are needed to determine the value of ginsenoside Rg3 for NSCLC patients with COVID-19.

• Journal of Korea Port Economic Association
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• v.34 no.4
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• pp.141-160
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• 2018

In this paper, to analyze the perception and response strategy of Korean ship owners on Global Sulphur Cap 2020, examined the IMO environmental regulation status focusing on MARPOL Annex VI regulation about air pollution prevention, technological measures to reduce SOx emission, shipping industry and management status of Korean ship owners. First of all, the questionnaire was conducted for Korean ship owners after selecting the evaluation factors. The purpose of this study was to investigate the difference of the perception and response strategy of Korean ship owners by corporation size and main vessel type using frequency and cross analysis. It is confirmed that various researches on SOx emission reduction have been carried out from various points of view at home and abroad. In this study, existing studies related to technical factors for regulatory response and economics analysis were examined and evaluation factors were selected. As a result of analysis, it is found that large-sized shipping companies are more prepared for regulatory response than small and medium-sized bulk carrier owners. There were similar perception and the direction of response strategy about the impacts by corporation size and main vessel type. In about two years to be implemented in 2020, It is necessary to find an appropriate response strategy based on the support policy of the government and related organizations and the systematic analysis of the ship owners. Through this study, although the difference between the perception and response strategy of the ship owners by corporation size and main vessel type was understood, it was found that there were limitations on specific response strategy and corporate data collection. In future research, we should overcome the limitations of this study and conduct an in-depth study.

• Journal of Korean Society of Transportation
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• v.22 no.2 s.73
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• pp.145-154
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• 2004

The growing demand for customer-responsive, made-to-order manufacturing is stimulating the need for improved dynamic decision-making processes in commercial fleet operations. Moreover, the rapid growth of electronic commerce through the internet is also requiring advanced and precise real-time operation of vehicle fleets. Accompanying these demand side developments/pressures, the growing availability of technologies such as AVL(Automatic Vehicle Location) systems and continuous two-way communication devices is driving developments on the supply side. These technologies enable the dispatcher to identify the current location of trucks and to communicate with drivers in real time affording the carrier fleet dispatcher the opportunity to dynamically respond to changes in demand, driver and vehicle availability, as well as traffic network conditions. This research investigates key aspects of real time dynamic routing and scheduling problems in fleet operation particularly in a truckload pickup-and-delivery problem under various settings, in which information of stochastic demands is revealed on a continuous basis, i.e., as the scheduled routes are executed. The most promising solution strategies for dealing with this real-time problem are analyzed and integrated. Furthermore, this research develops. analyzes, and implements hybrid algorithms for solving them, which combine fast local heuristic approach with an optimization-based approach. In addition, various partitioning algorithms being able to deal with large fleet of vehicles are developed based on 'divided & conquer' technique. Simulation experiments are developed and conducted to evaluate the performance of these algorithms.

• Journal of Periodontal and Implant Science
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• v.35 no.2
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• pp.511-524
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• 2005

The regeneration of lost periodontal tissue is a major goal of therapy. Periodontal ligament cell(PDL) is a specialized connective tissue that connects cementum and alveolar bone to maintain and support teeth in situ and preserve tissue homoeostasis. Bone morphogenetic proteins(BMPs) have shown much potential in the reconstruction of the periodontum by stimulate new bone and new cementum formation. Limitiations of BMP administration to periodontal lesions is high dose delivery, BMP transient biological activity, and low bioavailability of factors at the wound site. Gene delivery method can be alternative treatment strategy to deliver BMPs to periodontal tissue. The purpose of this study is to investigate efficiency of BMP-2 gene delivery with cell-based therapy using PDL cells. PDL cell were transduced with adenoviruses encoding either BMP-2 or Lac-Z gene. To evaluate osteogenic activity of expressed BMP-2 on PDL cells, we investigated secreted BMP-2, cellular activity, ALPase, produced mineralized nodules. To evaluate collagen scaffold as carrier for transduced cell delivery, we examined morphology and secreted BMP-2 of transducd PDL cells on it. BMP-2 transducd PDL cells produced higher levels of BMP-2, ALPase, mineralized nodules than non transduced cells. Cellular activity of transduced cells was showed similar activity to non transduced cells. Transduce cells attached on collagen scaffold secreted BMP-2 at 7day and was showed similar morphology to non transduced cells. These results demonstrated that transduced PDL cells produced biologically active BMP-2 and collagen scaffold could be carrier of transducd cells.