• 대한치과의사협회지
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• 제54권10호
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• pp.752-770
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• 2016

Introduction: Caries prevention through diet control can be achieved clinically by use of the cariogenic potentiality for a range of food commonly eaten by Koreans. Material & methods: The cariogenic potentiality index of each food can be calculated with the sugar contents and the viscosity of each food applying the regression analysis on the variables. 278 favorite foods for Korean were examined. Result 1. The formula of CPI is as below Cariogenic potentiality index (CPI) = 2.581343*sugar contents+0.598324*viscosity 2. The average CPI of the fruits, vegetable are 9.07~9.62 minutes. But the average CPI of the carbohydrate food is 15.21~19.60 minutes. Conclusion: Using the cariogenic potentiality indices, we will be able to determine an individual patients' average oral status for caries experience in the future. In addition, diet control by use of the cariogenic potentiality index and the analysis for each nutrient is considered as useful clinically for diet control in preventive dentistry.

• 한국치위생학회지
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• 제13권3호
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• pp.509-515
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• 2013

Objectives : This study was performed to evaluate the salivary secretion, salivary pH and cariogenic activity using unstimulated whole saliva in patients with hematologic malignancy. Methods : Nineteen patients (9 male, 10 female) who had hematologic malignancy and were treated with chemotherapy or bone marrow transplantation, and nineteen normal volunteers (7 male, 12 female) as control group were included. The mean age of patients group and control group was 45.1 and 46.7 years, respectively. Patients group was examined salivary secretion, salivary pH, and cariogenic activity using unstimulated whole saliva and was compared with control group. Results : In comparison with control group, salivary secretion, salivary pH and salivary buffer capacity were significantly lower in patients with hematologic malignancy (p<0.01). Both cariogenic activity(p<0.01) and the number of Lactobacilli(p<0.05) are higher in patients group than control group. Conclusions : These results suggest that the unstimulated whole salivary secretion, pH and buffer capacity were lower in patients with hematologic malignancy than control group. Cariogenic activity is higher in patients with hematologic malignancy than control group. Such salivary factor and cariogenic activity can increase the possibility of induction of dental caries.

• Journal of Microbiology and Biotechnology
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• 제26권11호
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• pp.1829-1835
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• 2016

Dental caries is caused by cariogenic biofilm, an oral biofilm including Streptococcus mutans. Recently, the prevention of dental caries using various probiotics has been attempted. Lactococcus lactis HY 449 is a probiotic bacterium. The aim of this study was to investigate the effect of L. lactis HY 449 on cariogenic biofilm and to analyze its inhibitory mechanisms. Cariogenic biofilm was formed in the presence or absence of L. lactis HY 449 and L. lactis ATCC 19435, and analyzed with a confocal laser microscope. The formation of cariogenic biofilm was reduced in cultures spiked with both L. lactis strains, and L. lactis HY 449 exhibited more inhibitory effects than L. lactis ATCC 19435. In order to analyze and to compare the inhibitory mechanisms, the antibacterial activity of the spent culture medium from both L. lactis strains against S. mutans was investigated, and the expression of glucosyltransferases (gtfs) of S. mutans was then analyzed by real-time RT-PCR. In addition, the sucrose fermentation ability of both L. lactis strains was examined. Both L. lactis strains showed antibacterial activity and inhibited the expression of gtfs, a nd t he d ifference b etween both strains did not show. In the case of sucrose-fermenting ability, L. lactis HY 449 fermented sucrose but L. lactis ATCC 19435 did not. L. lactis HY 449 inhibited the uptake of sucrose and the gtfs expression of S. mutans, whereby the development of cariogenic biofilm may be inhibited. In conclusion, L. lactis HY 449 may be a useful probiotic for the prevention of dental caries.

• 동의생리병리학회지
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• 제21권1호
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• pp.70-75
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• 2007

In the present study, we has been isolated the anti-cariogenic component, stigmasterol, from Aralia continentalis (A. continentalis) and identified by MS, $^1$H-NMR and $^{13}$C-NMR and also investigated the anti-cariogenic properties of stigmasterol. The methanol extract of ,A. continentalis showed concentration-dependent inhibitory activity against the growth and acid production of S. mutans. The MeOH extract was suspended in H$_2$O and sequentially partitioned with n-hexane, CHCl$_3$, EtOAc, and n-BuOH. The CHCl$_3$ fraction showed remarkable antibacterial activity against S. mutans. The anti-cariogenic compound, stigmasterol, has been isolated successively through the screening system and various chromatography methods. Anti-cariogenic properties of stigmasterol were also investigated. From this active chloroform subfraction, isolation and identification finally gave (24E)-stigmasta-5,22-dien-3${\beta}$-ol (stigmasterol) {[a]$_D\;^{25}$ -48.33$^{\circ}C$(C 0.28, CHCl$_3$)} by spectroscopic methods (MS, $^1$H-NMR and $^{13}$C-NMR) as an active principle. The compound, stigmasterol, showed significant growth, acid production, adhesion and water-insoluble glucan synthesis inhibitory effect against S. mutans. These results suggest that stigmasterol from ,A. continentalis may inhibit cariogenic properties of S. mutans and these properties may provide some scientific rationales that the local inhabitants used the extracts for treatment of dental diseases.

• 대한치과기공학회지
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• 제13권1호
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• pp.61-78
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• 1991

Dental caries is one of the main factors to cause the teeth to be lost. Diet is the important factor in the development of dental caries. Today, Dental plaque control, Fluoride to pical application, Fissure sealing and Diet control are used to prevent dental caries. By the five day diet diary, the author surveyed the food in take of 600 infants aged from 10 to 12 in the subures of Seoul. Using the cariogenic food intake analysis form, the detergent food intake analysis form and the basic food intake analysis form, the data were collected, analysed and discussed. After discussing the results, the author concluded as follows : 1. The intake frequency of meals per day was 4.91 times, of which 2.74 times taken at meals and 2.17 times at between meals. Girls(5.00) had taken more times than boys(4.69) at meals an between meals. 2. The intake frequency of cariogenic food per day was 1.93 times, Liquid cariogenic food was taken 0.05 times at meals, and 0.58 times at between meals. Solid cariogenic food was taken 0.05 times at meals, and 0.08 times at between meals. Girls(1.67) had taken cariogenic food more times than boys(1.46). 3. The percentage of intake without detergent food of each intake time per head per day was 71.62% at meals, and 85.7% at between meals. The highest percentage was at evening meals. Boys(44.00%) had taken more detergent food than girls(56.71%). 4. Both boys and girls had the basic food intake taken enough only in 2nd group of basic food, lacking in the other 4 groups. Girls had taken the basic food comparatively more times than boys.

• 한국학교보건학회지
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• 제5권2호
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• pp.91-103
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• 1992

The objective of school lunch program is make a student to understand the basic knowledge on the diet through improved food patterns. Diet is an important factor in the development of dental caries. Generally, snack foods contain much sugar and cause dental caries to children. The diet education in the school lunch program should involve the control of snack food intake. In this study, the anlysis of influence of school lunch program on the cariogenic snack food intake of school children was carried out. The questionarie was given to 1,441 sixth-grade children of 5 school lunch group and 1,443 sixth-grade children of 5 non-school lunch group in Pusan on the intake of snack foods and toothbrushing after meals. Its were as follows : 1. No differences were observed snack food intake per day between school lunch group and non-school lunch group. 2. The snack food intake frequency at home in school lunch group was more frequent than that of non-school lunch group. However, in school, the result was reverse. 3. No difference was observed the frequency between school lunch group on the intake of cariogenic, foods, detergent foods, protective foods, cariogenic foods alone and cariogenic and detergent foods between meals. 4. The frequency of toothbrushing per day and the ratio of toothbrushing after meals in school lunch group was a little higher than that of non-school lunch group. 5. No difference was observed on the ratio of toothbrushing after snack food intake between school lunch group and non-school lunch group. 6. The influence of school lunch program on the cariogenic food intake in school children was little significant for its goodness. 7. The improvement of education on snack food take and toothbrushing after meals to shool children was recommended.

• Natural Product Sciences
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• 제17권4호
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• pp.267-272
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• 2011

In our search for bioactive phenolics from plants, the culms of Phyllostachys bambusoides has been selected for investigation of anti-cariogenic and 1,1-diphenylpicrylhydrazyl (DPPH) radical scavenging agents based on the initial screening results. Fractionation process of n-hexane and $CHCl_3$ extracts afforded four phenolic constituents, ferulic acid (1), vanillin (2), coniferaldehyde (3), and coniferyl alcohol (4) as guided by their DPPH free radical scavenging activities. Additionally, activity-guided fractionation of EtOAc extract with anti-cariogenic activity has resulted in the isolation of coniferaldehyde (3), 2,6-dimethoxy-p-benzoquinone (5), p-methoxycinnamic acid (6), (${\pm}$)-balanophonin (7), and 6-methoxychromanone (8). The structures of 1 - 8 were determined by spectroscopic data interpretation, and also by comparison of their data with the published values. Phenolic compounds 1 - 4 exhibited similar DPPH radical scavenging activities compared with the synthetic antioxidant, butylated hydroxytoluene (BHT), and compounds 3 and 5 - 8 showed significant antibacterial activity against cariogenic oral streptococci, Streptococcus mutans and S. sobrinus.

• International Journal of Oral Biology
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• 제39권2호
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• pp.57-63
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• 2014

The purpose of this study is to evaluate salivary flow rate, salivary pH, and cariogenic activity using unstimulated saliva of the head and neck cancer patients. Twenty three cancer patients (19 males, 4 females) who had undergone chemotherapy and radiation therapy and twenty four healthy volunteers (14 males, 10 females) as a control were included. Salivary flow rate, salivary pH, and cariogenic activity using unstimulated saliva were examined. Compared to saliva of the control group, salivary flow rate (p<0.001) and salivary pH (p<0.001) were significantly lower in head and neck cancer patients. The colony counts of Lactobacilli was higher in head and neck cancer patients (p<0.05) than in control group. These salivary factors and cariogenic activity can increase the prevalence of dental caries in head and neck cancer patients.

• Journal of Korean Academy of Oral Health
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• 제43권3호
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• pp.118-123
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• 2019

Objectives: The purpose of this study was to investigate changes in the composition of artificial cariogenic biofilms using a Streptococcus mutans biofilm model over a period of time. Methods: We analyzed the dry weight, colony forming unit (CFU) number, extracellular polysaccharide (EPS) biovolume, and acid production rate of S. mutans biofilms formed on saliva-coated hydroxyapatite discs after 26 h, 50 h, 74 h, 98 h, 171 h, and 195 h. In addition, we performed a laser scanning confocal fluorescence microscopy to determine the bacterial volume, EPS biovolume, and biofilm thickness. We calculated the biofilm density using dry weight and EPS biovolume. Results: Over a period of time, there was no change in the CFU number and acid production rate of S. mutans biofilms, but there was an increase in the dry weight and EPS biovolume of S. mutans biofilms. The bacterial volume, EPS biovolume, and biofilm thickness only increased in the 50-h-old biofilm; however, no change was observed in 50-195-h-old biofilms. In addition, an increase in the biofilm density was observed over time. Conclusions: These results suggest that the acid production ability of cariogenic biofilms does not change, but the biofilm density increases over time. However, due to scientific information, further research needs to be conducted in the field of dentistry to get further insights on the progression of cariogenic biofilms over time.

• Archives of Pharmacal Research
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• 제15권3호
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• pp.239-241
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• 1992

The methanol extract of Cinnamoni Cortex showed antibacterial action against cariogenic bacterium, Streptococcus mutans OMZ 176. The active principle of the extract was identified to be trans-cinnamaldehyde, which was bactericidal in the minimal inhibitory concentration (MIC) of $100\;\mu$g/ml against the strain. From the results of antibacterial activity of cinnamaldehyde and its derivatives, the acrolein group in the cinnamaldehyde was elucidated to be an essential element for the activity.