• Animal Bioscience
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• v.36 no.6
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• pp.891-898
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• 2023

Objective: This study aimed to determine the effect of crude oligosaccharide extract from coconut milk meal (CMM) and spent tea leaves (ST) on the performance and gut development of broiler chickens. Methods: A total of 240 one-day-old unsexed broiler chicks (ROSS 308) were raised on litter-floored pens and had ad libitum access to water for 42 days. The experiment was conducted on chicks fed with basal diet (CON), commercial mannan-oligosaccharides (MOS), crude oligosaccharide extract from CMM, and crude oligosaccharide extract from ST. The experimental diets were supplemented with 2 and 1 g/kg oligosaccharides during the starter and grower periods, respectively. Results: The body weight gain (BWG) of birds in the MOS group was higher than that of birds in the other groups (p<0.05) in the starter period. However, during the grower period, ST significantly improved the BWG compared to the MOS (p<0.05). MOS, CMM, and ST showed no influence on the carcass and visceral organ weight and the weight and length of intestine (p>0.05). The digestibility of gross energy was greater (p<0.05) in the CMM group than in the CON group during the grower period. Morphological changes were absent in the dietary treatments (p>0.05). Conclusion: The improvements in the growth performance were partly driven by nutrient digestibility of such oligosaccharides having prebiotic properties. This result can indicate that supplementing broiler diets with crude oligosaccharides from CMM and ST had no negative effect on the growth performance and gut development of broilers.

• Korean Journal of Agricultural Science
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• v.48 no.4
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• pp.957-963
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• 2021

The study aimed to investigate the effects of supplemented sodium butyrate on the in vitro rumen fermentation and growth performance of Hanwoo calves. In total, four treatments were employed according to the sodium butyrate levels: no addition (control), an addition of 0.1% (treatment 1), an addition of 0.3% (treatment 2), and an addition of 0.5% (treatment 3). After 48 hours of fermentation, the ruminal pH was found to be higher in T1 than in C. Total volatile fatty acids were significantly higher in T2 and T3 than in C. The ratio of acetate and propionate was significantly lower in T1 and T3 than in C. In this study, the optimal concentration to promote rumen fermentation was found to be 0.3%, i.e., T2, and an experiment on Hanwoo calves at a farm was conducted. However, there were no significant differences between the treatment groups in terms of the daily weight gain, feed conversion ratio, and final body weight in the feeding experiment. Also, there were no significant differences in the body length, withers height, and height at hip cross between the control and the treatment groups. The addition of 0.3% sodium butyrate was most effective at promoting in vitro rumen fermentation, but it did not significantly affect the growth performance when fed to Hanwoo calves. This indicates that the addition of sodium butyrate improved rumen fermentation but did not have a growth-promoting effect. Future studies need to compare growth and carcass performance outcomes to confirm long-term effects.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.4
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• pp.549-553
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• 2006

In this study, the effects of granite-grit offered free-choice on voluntary intakes of grit and subsequently on some morphologic traits of the digestive tract and performance of broilers were investigated. A total of 240 7d-old male broiler chicks (Ross 308) were allotted randomly to 10 floor pens supplied with wood shavings. The experiment lasted for 42 days. There were 2 dietary treatments, each consisting of 5 replicates. Each replicate consisted of 24 birds kept in an experimental unit with a floor size of $2{\times}2m$. Dietary treatments consisted of control (C) in which broilers were fed standard broiler rations, and acid insoluble granite-grit choice (AIGG) in which broilers were fed standard broiler rations and grit in separate troughs. Mean amounts consumed varied quite widely from week to week, but on average broilers ate 3.41 g per d per bird during the experimental period. Birds had a higher voluntary intake of granite-grit at an early age (7 to 21 d of age) than later (22 to 42 d of age). The voluntary intake of granite-grit of AIGG broilers increased (p<0.05) from 2.7 g/day at 7-14 d to 4.4 g at 15-21 d of age, and then it decreased to 3.4, 3.2 and 3.4 g/day between 22-28, 29-35 and 36-42 d of age, respectively. This level of grit intake increased (p<0.05) weights of empty gizzard (0.97 vs. 1.30 g), edible inner organs (3.51 vs. 3.69 g), and length of gut (8.86 vs. 9.01 cm) as a proportion of body weight and the content of insoluble ash (8.4 vs. 42.2 g/kg) in the faeces compared to the control group. Feeding free-choice grit had little or no effect on final live weights (2,542 vs. 2,543 g), daily gains (69 vs. 69 g), carcass weights (1,924 vs. 1,911), dressing percentages (75.6% vs. 75.1%) and feed efficiencies (1.69 vs. 1.66). Birds given grit did not gain more weight than those not given grit but they tended to have (p<0.07) lower feed intake (116.7 vs. 114.5 g), and consequently lower protein and energy intake. In conclusion, the granite-grit consumed voluntarily by broilers increased gut length and empty gizzard weight without affecting growth performance of broilers. Thus, it can be assumed that the voluntary consumption of granite-grit was too low to affect performance.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.9
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• pp.1319-1326
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• 2007

Fructose-1,6-bisphosphatase (FBP1) is a key regulatory enzyme of gluconeogenesis that catalyzes the hydrolysis of fructose-1,6-bisphosphate to generate fructose-6-phosphate and inorganic phosphate. Deficiency of fructose-1, 6-bisphosphatase is associated with fasting hypoglycemia and metabolic acidosis. The enzyme has been shown to occur in bacteria, fungi, plants and animals. The bovine FBP1 gene was cloned and characterized in this study. The full length (1,241 bp) FBP1 mRNA contained an open reading frame (ORF) encoding a protein of 338 amino acids, a 63 bp 5' untranslated region (UTR) and a 131 bp 3' UTR. The bovine FBP1 gene was 89%, 85%, 82%, 82% and 74% identical to the orthologs of pig, human, mouse, rat and zebra fish at mRNA level, and 97%, 96%, 94%, 93% and 91% identical at the protein level, respectively. This gene was broadly expressed in cattle with the highest level in testis, and the lowest level in heart. An intronic single nucleotide polymorphism (SNP) (A/G) was identified in the $5^{th}$ intron of the bovine FBP1 gene. Genotyping of 133 animals from four beef breeds revealed that the average frequency for allele A (A-base) was 0.7897 (0.7069-0.9107), while 0.2103 (0.0893-0.2931) for allele B (G-base). Our preliminary association study indicated that this SNP is significantly associated with traits of Average Daily Feed Intake (ADFI) and Carcass Length (CL) (p<0.01). In addition, the FBP1 gene was assigned on BTA8 by a hybrid radiation (RH) mapping method.

• Animal Bioscience
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• v.35 no.7
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• pp.1059-1068
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• 2022

Objective: This study was conducted to evaluate the effect of using low energy diet with multi-enzymes supplementation on different biological parameters in broilers. Methods: Three hundred Arbor Acres broiler chicks were randomly divided into three groups (Cont, standard metabolizable energy(ME); L-ME, ME reduced by 50 kcal/kg without enzyme; and L-ME-MES, L-ME diet was supplemented with multi-enzymes) with five replicates per group (20 chicks per replicate) at the start of second week. Grower and finisher diets were formulated according to breed specific guide and offered with free access in respective phase (two weeks for grower [8 to 21 d]; two weeks for finisher [22 to 35 d]). External marker method was used to measure the nutrient digestibility. After feeding trial, fifteen birds (one bird per replicate) were selected randomly and slaughtered for samples collection. Results: The results exhibited no effect (p>0.05) of dietary treatments on all parameters of growth performance, carcass traits, relative weight of internal organs except bursa and overall parameters of thigh meat quality. Relative weight of bursa was significantly (p<0.05) higher in L-ME than control. Multi-enzymes supplementation in low-ME diet significantly (p<0.05) improved the breast meat pH 24 h, digestibility of crude protein, duodenum weight and length, jejunal morphology, counts of Lactobacillus spp. and Bifidobacterium spp., lipase and protease activities than control. Jejunum length was increased in both L-ME and L-ME-MES treatments than that of the control (p<0.05). Breast meat cooking loss and color lightness was lower in L-ME (p<0.05) than control. Conclusion: It can therefore be concluded that broilers could be reared on low energy diet with supplementation of multi-enzymes without compromising the growth performance. In addition, it is beneficial for other biological parameters of broilers.

• Journal of Animal Science and Technology
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• v.47 no.6
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• pp.1017-1024
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• 2005

This study was investigated the effect of the length of stunning time on pH, water holding capacity(WHC), cooking loss(CL), meat color and incidence rate of blood spot in broiler carcass. One-hundred and forty broiler chickens were slaughtered by three different length of stunning times(5 sec., 8 sec., 11 sec.) with the same electrical frequency(255Hz) and 50 voltage in a commercial abattoir. The WBS values and cooking loss of breast muscle were increased with increasing the stunning time, while WHC of breast muscle were decreased. Lightness(L* value) and yellowness(b* value) scores of leg muscle and skin stunned with 50V, 255Hz, 8 sec, 11 sec. were higher than those of broilers stunned with 50V, 255Hz, 5sec(P<0.05). But, there was no significant difference in color on breast and wing muscle. In subjective evaluation, frequence of the first grade had a trend of being increased by extending the stunning time. There was only a few cases of PSE chicken with 0.02%, while blood spot was observed at the highest rate for the 5 sec. treatment. TBARS indicated that a longer length of stunning resulted in a higher rate of fat oxidation. This experiment demonstrated that the length of stunning time has a significant effect on meat quality and its stability during chiller storage.

• Journal of Aquaculture
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• v.21 no.4
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• pp.272-277
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• 2008

We investigated the growth and survival in seedling production, and growth performance was compared with the different rearing tanks and protein source of formulated feed for juvenile Pacific bluefin tuna Thunnus orientalis (PBT). The survival rate at the end of nursery culture at 30 days after hatching was $0.69{\pm}0.40%$, and total length and mean body weight were $49.83{\pm}2.52\;mm$ and $1.03{\pm}0.09\;g$, respectively. Growth performance has no significant difference in fish reared by different tanks forms for 10 days. In order to develop an artificial diet, we evaluated the dietary utility of enzyme treated fish meal (Bio-CP, BIO) for juvenile PBT. Only diet BIO sustained similar growth and higher feed efficiency, and final carcass lipid content as compared to those of Sand lance (SL) These results revealed that BIO-CP a suitable dietary protein source, could sustain growth of PBT.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.11
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• pp.1755-1762
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• 2020

Objective: Thyroid hormone responsive spot 14 alpha (THRSP) has been used to investigate the regulation of de novo lipogenesis because the variation of THRSP mRNA content in the tissue affects directly the ability of that tissue to synthetize lipids. Also, this gene responds to thyroid hormone stimulation and high level of carbohydrate feeding or insulin-injection. This study was carried out to investigate variations within THRSP and their effects on body and carcass weights in Korean native chicken (KNC). Methods: A total of 585 chickens which represent the five lines of KNC (Black, Gray-Brown, Red-Brown, White, and Yellow-Brown) were reared and body weight data were recorded every two weeks from hatch until 20 weeks of age. Polymerase chain reaction- restriction fragment length polymorphism, DNA chips for Agilent 2100 Bioanalyzer, and Fluidigm Genotyping Technology, were applied to genotype selected markers. A linear mixed-effect model was used to access association between these single nucleotide polymorphism (SNP) markers and growth-related traits. Results: A total of 30 polymorphisms were investigated in THRSP. Of these, nine SNPs for loci were selected to perform association analyses. Significant associations were detected between g.-49G>T SNP with body weight at 20 weeks of age (BW20), g.451T>C SNP with growth at 10 to 12 weeks of age (GR10-12), and g.1432A>C SNP with growth at 14 to 16 weeks trait (GR14-16) and body weight at 18 weeks of age (BW18). Moreover, diplotype of the THRSP gene significantly affected body weight at 12 weeks of age (BW12) and GR10-12 traits. Diplotype of ht1/ht2 was favorable for BW12 and GR10-12 traits. Conclusion: These results suggest that THRSP can be regarded as a candidate gene for growth traits in KNC.

• Journal of agriculture & life science
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• v.50 no.4
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• pp.113-125
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• 2016

This study was conducted to investigate the effect of low feeding level of concentrate after sexual maturity(14 month old) on the growing and breeding performances in Hanwoo cows. Eighteen cows(average body wt. 251kg) were alloted to two groups by completely randomized design, and fed a commercial formula feed by 0.8%(T1) and 1.2%(T2) of body weight for 30 months of total experimental period(feeding trial of 20 months, breeding trial of 10 months). When the results of this experiment compared to the Korean feeding standard for Hanwoo(2012), the reason of low growing performance in T1 was induced from low protein intake rather than energy. The change of body size by feeding levels was significantly appeared at chest width and body length, particularly chest width of cows in T1 was rather reduced by 3.7% in the final than the initial measurement. Ovarian size of T1 was only 63% of T2, and pregnancy rate(33.3% : 77.8%) and birth wight of calf(22.5kg : 29.5kg) were also seriously repressed by low intake of dietary protein after sexual maturity in Hanwoo cow. It was appeared that the growing and breeding performances of Hanwoo cows were more affected by the level of dietary protein rather than energy in the feeding after sexual maturity.

• Journal of Animal Science and Technology
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• v.45 no.6
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• pp.939-948
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• 2003

The objective of this study was to identify the quantitative traits loci(QTL) for economically important traits such as growth, carcass and meat quality on pig chromosome 6. A three generation resource population was constructed from cross between Korean native boars and Landrace sows. A total of 240 F$_2$ animals were produced using intercross between 10 boars and 31 sows of F$_1$ animals. Phenotypic data including body weight at 3 weeks, backfat thickness, muscle pH, shear force and crude protein level were collected from F$_2$ animals. Animals including grandparents(F$_0$), parents(F$_1$) and offspring(F$_2$) were genotyped for 29 microsatellite markers and PCR-RFLP marker on chromosome 6. The linkage analysis was performed using CRI-MAP software version 2.4(Green et al., 1990) with FIXED option to obtain the map distances. The total length of SSC6 linkage map estimated in this study was 169.3cM. The average distance between adjacent markers was 6.05cM. For mapping of QTL, we used F$_2$ QTL Analysis Servlet of QTL express, a web-based QTL mapping tool(http://qtl.cap.ed.ac.uk). Five QTLs were detected at 5% chromosome-wide level for body weight of 3 weeks of age, shear force, meat pH at 24 hours after slaughtering, backfat thickness and crude protein level on SSC6.