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http://dx.doi.org/10.5187/JAST.2003.45.6.939

Linkage Map and Quantitative Trait Loci(QTL) on Pig Chromosome 6  

Lee, H.Y. (National Livestock Research Institute, RDA)
Choi, B.H. (National Livestock Research Institute, RDA)
Kim, T.H. (National Livestock Research Institute, RDA)
Park, E.W. (National Livestock Research Institute, RDA)
Yoon, D.H. (National Livestock Research Institute, RDA)
Lee, H.K. (Department of Biotechnology, Hankyong National University)
Jeon, G.J. (Department of Biotechnology, Hankyong National University)
Cheong, I.C. (National Livestock Research Institute, RDA)
Hong, K.C. (Division of Biotechnology and Genetic Engineering, College of Life and Environmental Sciences Korea University)
Publication Information
Journal of Animal Science and Technology / v.45, no.6, 2003 , pp. 939-948 More about this Journal
Abstract
The objective of this study was to identify the quantitative traits loci(QTL) for economically important traits such as growth, carcass and meat quality on pig chromosome 6. A three generation resource population was constructed from cross between Korean native boars and Landrace sows. A total of 240 F$_2$ animals were produced using intercross between 10 boars and 31 sows of F$_1$ animals. Phenotypic data including body weight at 3 weeks, backfat thickness, muscle pH, shear force and crude protein level were collected from F$_2$ animals. Animals including grandparents(F$_0$), parents(F$_1$) and offspring(F$_2$) were genotyped for 29 microsatellite markers and PCR-RFLP marker on chromosome 6. The linkage analysis was performed using CRI-MAP software version 2.4(Green et al., 1990) with FIXED option to obtain the map distances. The total length of SSC6 linkage map estimated in this study was 169.3cM. The average distance between adjacent markers was 6.05cM. For mapping of QTL, we used F$_2$ QTL Analysis Servlet of QTL express, a web-based QTL mapping tool(http://qtl.cap.ed.ac.uk). Five QTLs were detected at 5% chromosome-wide level for body weight of 3 weeks of age, shear force, meat pH at 24 hours after slaughtering, backfat thickness and crude protein level on SSC6.
Keywords
QTL Mapping; Resource population; Economic traits; Linkage mapping; Pigs;
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1 Barendse, W., Vaiman, D., Kemp, S. J., Sugimoto, Y., Armitage, S. M., Williams, J. L., Sun, H. S., Eggen, A., Agaba, M., Aleyasin, S. A. and Band, M. 1997. A medium-density genetic linkage map of the bovine genome. Mamm Genome 8:21-28.
2 Bidanel, J. P., Milan, D., Iannuccelli, N., Amigues, Y., Boscher, M. Y., Bourgeois, F., Caritez, J. C., Gruand, J., Le Roy, P., Lagant, H., Quintanilla, R., Renard, C., Gellin, J., Ollivier, L. and Chevalet, C. 2001. Detection of quantitative trait loci for growth and fatness in pigs. Genet Sel Evol 33:289-309.
3 Geldermann, H., Mueller, E., Beeckman, P. and Knoor, C. 1996. Mapping Quantitative trait loci by means of marker genes in F$_2$ generations of Wild Boar, Pietrain and Meishan pigs. J Anim Breed Genet 113:381-387.
4 Bishop, M. D., Kappes, S. M., Keele, J. W., Stone, R. T., Sunden, S. L., Hawkins, G. A., Toldo, S. S., Fries, R., Grosz, M. D., Yoo, J. and Beattie, C. W. 1994. A genetic linkage map for cattle. Genetics 136:619-639.
5 Friedman J. F. and Halaas J. L. 1998. Leptin and the regulation of body weight in mammals, Nature 395. 763-770.
6 Fujii, J., Otsu, K., Zorzto, F., de Leon, S., Khanna, V. K., Weiler, J. E., O'Brien, P. J. and MacLennan, D. H. 1991. Identification of a mutation in porcine ryanodine receptor associated with malignant hyperthermia. Science 253:448-451.   DOI
7 Green, P., Falls, K. and Crooks, S. 1990. Documentation for CRIMAP, version 2.4. Washington Univ. School of Medicine, St. Louis, MO.
8 Haley, C. S., Knott, S. A. and Elsen, J. M. 1994. Mapping quantitative trait loci in crosses between outbred lines using least squares. Genetics 136:1195-1207.
9 Andersson-Eklund, L., Marklund, L., Lundstrom, K., Haley, C. S., Andersson, K., Hansson, I., Moller, M. and Andersson, L. 1998. Mapping quantititative trait loci for carcass and meat traits in a Wild boar${\times}$Large White intercross. J Anim Sci 76:694-700.
10 Andersson, L., Haley, C. S., Ellegren, H., Knott, S. A., Johansson, M., Andersson, K., Andersson-Eklund, L., Edfors-Lilja, I., Fredholm, M., Hansson, I., Hakansson, J. and Lundstrm, K. 1994. Genetic mapping of quantitative trait loci for growth and fatness in pigs. Science 263:1771-1774.
11 Archibald, A. L., Haley, C. S., Brown, J. F., Couperwhite, S., McQueen, H. A., Nicholson, D., Coppieters, W., Vandeweghe, A., Stratil, A. and Wintero, A. K. 1995. The Pigmap Consortium linkage map of the pig (Sus scrofa). Mamm Genome 6:157-175.   DOI   ScienceOn
12 Barendse, W., Armitage, S. M., Kossarek, L. M., Shalom, A., Kirkpatrick, B. W., Ryan, A. M., Clayton, D., Li, L., Neibergs, H. L. and Zhang, N. 1994. A genetic linkage map of the bovine genome. Nat Genet 6:227-235.
13 Morales J., Perez J. F., Baucells M. D., Gasa A. and Gasa J. 2000. Comparative digestibility and productive performances between Landrace and Iberian pigs fed on corn or a sorghum-acorn-based diet, in; 8th Symposium on Digestive Physiology in Pigs. Uppsala, Sweden, June 20-22. CABI Publishing. Ed. Linberg and Bogle, 2000. pp. 227-229.
14 Ovilo C., Perez-Enciso M., Barragan C., Clop A., Rodriguez C., Oliver M. A., Toro M. A. and Noguera J. L. 2000. A QTL for intramuscular fat and backfat thickness is located on porcinc chromosome 6. Mamm Genome 11:344-346.
15 Paszek, A. A., Wilkie, P. J., Flickinger, G. H., Rohrer, G. A., Alexander, L. J., Beattie, C. W. and Schook, L. B. 1999. Interval mapping of growth in divergent swine cross. Mamm Genome 10:117-122.   DOI   ScienceOn
16 De Koning, D., Harlizius, B., Rattink, A. P., Bovenhuis, H., Groenen, M. A. M. and Brascamp, W. E. 2000. Detection and characterization of quantitative trait loci for carcass and meat quality traits in pigs. Proc Europ Assoc Anim Prod 6:3.
17 Cameron, N. D. 1990. Comparison of Duroc and British Landrace pigs and the estimation of genetic and phenotypic parameters for growth and carcass traits. Animal Production 50:141.
18 Churchill G. A. and Doerge R. W. 1994. Empirical threshold values for quantitative trait mapping. Genetics 138, 963-971
19 De Koning, D. J., Janss, L. L. G., Rattink, A. P., van Oers, P. A. M., de Vries, B. J., Groenen, M. A. M., der Poel, J. J., de Groot, P. N., Brascamp, E. W. and van Arendonk, J. A. M. 1999. Detection of quantitative trait loci for back fat thickness and intramuscular fat content in Pigs (Sus scrofa). Genetics 152:1679-1690.
20 De Koning, D., Harlizius, B., Rattink, A. P., Groenen, M. A. M., Brascamp, E. W. and van Arendonk, J. A. M. 2001. Detection and characterization of quantitative trait loci for meat quality traits in pigs. J Anim Sci 79:2812-2819.
21 Devol. D. L., Mckeith, F. K., Bechtel, R. S., Novakofski. J., Shanks. R. D. and Carr, T. R. 1988. Variation in composition and palatability traits and relationships between muscle characteristics and palatability in a random sample of pork carcasses. Journal of Animal Science 66:385.
22 Ellegren, H., Chowdhary, B. P., Johansson, M., Marklund, L., Fredholm, M., Gustavsson, I. and Andersson, L. 1994. A primary linkage map of the porcine genome reveals a low rate of genetic recombination. Genetics 137:1089-1100.
23 이학교, 전광주. 2002. 돼지의 QTL 검색을 위한 유의적 임계수준(Threshold) 결정. 한국동물자원과학회지, 44(1):31-38.
24 Hardge T., Siebel K., Koepke K. and Wimmers K. 2000. Association between Leptin(LEP)/Leptin receptor(LEPR) polymorphisms and fatness related traits in a porcine resource family, in: 27th International Conference on Animal Genetics, Minnesota, July 22-26, 2000, Conference Abstract Book, International Society of Animal Genetics, University of Minesota, Minnesota, p65.
25 Marklund, L., Nystrom, P., Stren, S., Andersson, L. and Andersson, E. L. 1999. Confirmed quantitative trait loci for fatness and growth on pig chromosome 4. Heredity 82: 134-141.   DOI   ScienceOn
26 Hodgson. R. R., Belk, K. E., Savell, J. W., Cross, H. R. and Williams, F. L. 1992. Development of a quantitative qulity grading system for mature cow carcass. J. Anim. Sci. 70:1840.
27 Malek, M., Dekkers, J. C. M., Lee, H. K., Baas, T. and Rothschild, M. F. 2001. A molecular genome scan analysis to identify chromosomal regions influencing economic traits in the pig. II. Meat and muscle composition. Mamm Genome 12:637-645.
28 Marklund, L., Johansson Moller, M., Hoyheim, B., Davies, W., Fredholm, M., Juneja, R. K., Mariani, P., Coppieters, W., Ellegren, H. and Andersson, L. 1996. A comprehensive linkage map of the pig based on a wild pig-Large White intercross. Anim Genet 27:255-269.
29 Mikawa, S., Akita, T., Hisamatsu, N., Inage, Y., Ito, Y., Kobayashi, E., Kusumoto, H., Matsumoto, T., Mikami, H., Minezawa, M., Miyake, M., Shimanuki, S., Sugiyama, C., Uchida, Y., Wada, Y., Yanai, S. and Yasue, H. 1999. A linkage map of 243 DNA markers in an intercross of Gottingen miniature and Meishan pigs. Anim Genet 30:407-417.
30 Serra X., GilF., Perez-Enciso M., Oliver M. A., Vazquez, J. M., Gispert M., Diaz I., Moreno F., Latorre R. and Noguera J. L. 1998. A comparison of carcass meat quality and histochemical characteristics of Iberian (Guadyerbas line) and Landrace pigs. Livest. Prod. Sci. 56. 215-223.
31 Wang, L., Yu, T. P., Tuggle, C.K., Liu, H. C. and Rothschild, M. F. 1998. A directed search for quantitative trait loci on chromosomes 4 and 7 in the pig. J Anim Sci 76:2560-2567.   DOI
32 Rohrer, G. A., Alexander, L. J., Hu, Z., Smith, T. P. L., Keele, J. W. and Beattie, C. W. 1996. A comprehensive map of the porcine genome. Genome Res 6:371-391.   DOI   ScienceOn
33 Perez-Enciso M., Clop, A., Folch, J. M., Sanchez, A., Oliver, M. A., Ovilo, C., Barragan, C., Varona, L. and Noguera, J. L. 2002. Exploring Alternative Models for Sex-Linked Quantitative Trait Loci in Outbred Population. Application to an iberian${\times}$landrace pigs intercross. Genetics Aug;161(4):1625-32.
34 Rattink, A. P., De Koning, D. J., Faivre, M., Harlizius, B., van Arendonk, J. A. M. and Groenen, M. A. M. 2000. Fine mapping and imprinting analysis for fatness trait QTLs in pigs. Mamm Genome 11:656-661.
35 Rohrer, G. A., Alexander, L. J., Keele, J. W., Smith, T. P. and Beattie, C. W. 1994. A microsatellite linkage map of the porcine genome. Genetics 136:231-245.
36 Rohrer, G. A. and Keele, J. 1998a. Identification of quantitative trait loci affecting carcass composition in swine I. Fat deposition traits. J Anim Sci 76:2247-2254.
37 Rohrer, G. A. and Keele, J. 1998b. Identification of quantitative trait loci affecting carcass composition in swine II. Muscling and wholesale product trait yield traits. J Anim Sci 76:2255-2262.
38 Rohrer, G. A. 2000. Identification of quantitative trait loci affecting birth characters and accumulation of back fat and weight in Meishan-White Composit resource population. J Anim Sci 78:2547-2553.   DOI