• Title/Summary/Keyword: Carboxymethyl cellulose (CMC)

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Confirmation of Non-Siderophore Antifugal Substance and Cellulase from Bacillus lichemiformis Kll Containing Antagonistic Ability and Plant Growth Promoting Activity (생물방제능과 식물성장촉진능을 동시에 가지는 Bacillus licheniformis K11의 non-siderophore 항진균 물질 및 cellulase의 생산조건 확인)

  • Woo, Sang-Min;Kim, Sang-Dal
    • Journal of Life Science
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    • v.17 no.7 s.87
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    • pp.983-989
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    • 2007
  • Bacillus lichemiformis Kll, a plant growth promoting rhizobacterium was reported as a producer of auxin, siderophore, as well as antifungal cellulase under some culture conditions. In vitro test, B. licheniformis Kll represented excellent antagonistic ability against Fusarium oxyspoum (KACC 40037), and showed broad spectrum against other phytopathogenic fungi. B. licheniformis Kll had cellulolytic activity toward not only carboxymethyl-cellulose (CMC) but also insoluble cellulose, such as fungal cell wall cellulose, filter paper (Whatman No. 1), and Avicel. In addition, we confirmed antifungal substance production by butanol-extract methods. The strain produced optimally the antifungal substance when it was cultivated at pH 9.0, 30${\circ}$C for 4 days on nutrient medium. The biological control mechanisms of B. lichemiformis Kll were caused by antifungal substance, cellulase and siderophore against phytopathogenic fungi.

Studies on Cellulolytic Enzymes Produced by Pleurotus spp. in Synthetic Medium( I ) -Effects of Carbon and Nitrogen Sources- (합성배지(合成培地)에서 Pleurots속(屬)이 생산(生産)하는 섬유소(纖維素) 분해효소(分解酵素)에 관한 연구(硏究)(제1보)(第1報) -탄소원(炭素源)과 질소원(窒素源)의 영향(影響)-)

  • Hong, Jai-Sik;Lee, Jong-Bae;Koh, Moo-Seok;Kim, Jeong-Sook;Lee, Keug-Ro;Kim, Myung-Kon
    • The Korean Journal of Mycology
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    • v.13 no.4
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    • pp.213-219
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    • 1985
  • Among the eight strains, Pleurotus sajor-caju JAFM 1017 was selected as most potent producer of cellulolytic enzymes. The avicelase and CMCase activity reached maximum levels after 10 days, and ${\beta}-glucosidase$ activity reached a maximum level after 19 days. Among the various carbon sources, cellulose powder was most effective for the production of avicelase and ${\beta}-glucosidase$, and Na-CMC (sodium carboxymethyl cellulose) was good for the production of CMCase. The optimum concentration of cellulose powder was 1.0% (w/v), and glucose (1.0%) completely depressed the production of enzymes. Nitrates were effective for the production of enzymes, but nitrites did not support growth. The production of cellulolytic enzymes increased as the concentration of urea increased. The appropriate concentration of urea was 0.054% (w/v).

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Isolation and Characterization of Marine Microorganisms Producing Cellulase from the Seashore of the Kyungsang Province in Korea

  • Jo, Kang-Ick;Lee, Bo-Hwa;Kim, Bo-Kyung;Jo, Hae-Young;Kim, Sung-Koo;Nam, Soo-Wan;Lee, Jin-Woo
    • 한국생물공학회:학술대회논문집
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    • 2005.10a
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    • pp.307-311
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    • 2005
  • Marine microorganisms to produce functional biopolymers were isolated from the seashore of the Kyungsang province. Microorganisms to hydrolyze carboxy-methyl cellulose(CMC) were cultured in marin broth and the other liquid medium that contained 2.0% (w/v) glucose, 0.25% yeast extract, 0.5% $K_2HPO_4$, 1% NaCl, 0.02% $MgSO_4{\cdot}7H_2O$ and 0.06% $(NH_4)_2SO_4$ to investigate the ability to produce carboxymethyl cellululase (CMCase) under aerobic conditions. Twelve microorganisms among them showed higher activities of CMCase than B. amyloliquefaciens DL-3, which was known as a cellulase-producing strain. The microorganism showing highest activity of CMCase in this study was identified as Bacillus subtilis subsp. subtilis with 16S rDNA partial sequencing and gyrase A partial sequencing and named as B. subtilis subsp. subtilis A-53.

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Effects of the Water Quality on the Dispersion Properties of Bentonites Used for Drilling Fluid (시추이수용 벤토나이트의 분산 특성에 미치는 수질의 영향)

  • Akther, Shamima;Hwang, Jin-Yeon;Lee, Hyo-Min
    • Journal of the Mineralogical Society of Korea
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    • v.20 no.1 s.51
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    • pp.21-33
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    • 2007
  • The dispersion/flocculation behavior of bentonite is a major concern in performance of drilling fluid. We studied the dispersion/flocculation characteristics of three commercial bentonites [two CMC (carboxymethyl cellulose) treated and one untreated] in waters of different pHs and salt concentrations. We also examined changes in the viscosity of bentonite suspensions in such waters as a major rheological property of drilling fluid. The dispersion/flocculation behaviors of bentonites were measured by two methods: colorimetric and light scattering method. Light scattering method allows estimating the floc diameter and flocculation rate. The dispersion and flocculation behaviors were diverse with the different bentonites and water qualities. In distilled water, all the bentonites were well dispersed up to first 10 minutes. After that, the CMC-bearing bentonites were flocculated. In salt waters, all the samples were flocculated and the flocculation rate is varied with salt concentration and polymer content. The volume of settled flocs decreased with increasing salt concentration. The flocculation rate and floc diameter increased with decreasing pH of solutions, whereas the volume of settled flocs increased with increasing pH of solutions. The bentonites of fast flocculation behavior had low viscosity. The results of the present study will be helpful in applying bentonites to drilling fluids in diverse environments.

Selection of Binder and Solid Materials for Pelleting Welsh Onion(Allium fistulosum L.) Seeds (파 펠렛종자 생산을 위한 접착제 및 피복재료 선발)

  • 강점순
    • Journal of Life Science
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    • v.12 no.6
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    • pp.721-730
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    • 2002
  • This study was carried out to select new pelleting binder and material for Welsh onion seeds. The optimum treatments of the various plant growth regulators to improve seed germination of the Welsh onion was also estimated. There were no significant effects of growth regulators on the germination percentage, but germination was faster according to the number of days to 50% of the final germination ($T_{50}$) and the mean number of days to germination (MDG) than those of the control. Germinability was increased when the seeds were soaked in gibberellic acid ($GA_3$) solution for 24hrs, even though there was no synergy effect on the germinability when $GA_3$ was mixed with 6-benzylaminopurine (BAP). The optimum treatment for improving germination of Welsh onion was observed when the tested seeds was soaked in 500 $\mu$M of $GA_3$ at $20^{\circ}C$ for 24hrs. Also, when the seeds soaked in the aforementioned treatment, the rate of germination was increased at lower temperature than at $20^{\circ}C$, the optimal temperature. The percentage and the speed of seed germination depended on the kinds of pelleting binder and their concentration. It showed that the higher the concentration of the binder for seeds pelleting, the lower the percentage of seed germination. Among the pelleting binder, polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP) were the best binders for seed pelleting, because seed germination using those binder did not affect on the concentration of binder. On the other hand, Carboxymethy cellulose (CMC) and methyl cellulose (MC) severely inhibited the seed germination The germinability was also different arcording to the pelleting materials. Among the different 58 pelleting materials, kaoline alone, the mixture of bentonite and kaoline, the mixture of bentonite, calcium carbonate and diatomaceous earth #300 were found as the best pelleting materials for welsh onion seeds.

Effects of Lignocellulosic Growing Media to The Prevention of Forest Soil Erosion

  • Jo, Jong-Soo;Ha, Si Young;Jung, Ji Young;Kim, Ji-Su;Nam, Jeong Bin;Yang, Jae-Kyung
    • Journal of the Korean Wood Science and Technology
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    • v.45 no.4
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    • pp.419-431
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    • 2017
  • The forest slopes cause substantial local changes in soil properties and an increase in soil erosion after extreme rainstorms. The high soil erosion rates on forest slopes need the effective use of growing media to control the soil runoff. Therefore, we prepared six different lignocellulosic growing media such as peat, perlite, and wood meal as the base materials and carboxymethyl cellulose (CMC), glucomannan, starch, old corrugated containerboard, and computer printout as the additional materials for the prevention of simulated rainfall-induced runoff. The growing media containing old corrugated containerboard efficiently reduced the percentage of soil runoff; however, it could not completely cushion the influence of crust. The best results for plant growth, except in the leaf area, were also obtained with the growing media containing old corrugated containerboard, suggesting an interesting way of paper recycling and an economic benefit for plant or crop growth in forest slope.

Influence of the Binder Types on the Electrochemical Characteristics of Si-C Composites Electrode in LIBs (Si-C 복합체 전극의 바인더 종류에 따른 전기화학적 특성 변화)

  • Jung, Sung-Hun;Ji, Mijung;Park, Geunyeong;Hong, Jongill;Choi, Byung-Hyun
    • Journal of the Korean Ceramic Society
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    • v.50 no.2
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    • pp.168-172
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    • 2013
  • This work presents the effects of binders on the electrochemical performance of Si-C composites as the anode of lithium ion batteries. PAI (polyamide-imide) was used as an organic binder, and PAN (polyacrylonitrile), PAA (polyacrylic acid) and CMC + SBR (carboxymethyl cellulose + styrene-butadiene rubber) were used as aqueous binders. As a result, stabilization time for the cell with a Si-C composite anode synthesized using aqueous binders became shorter than an organic binder. Particularly in the case of the cell using PAA binder, better performance was observed in terms of adhesion strength, initial efficiency, the volume expansion ratio, Coulombic efficiency, and capacity retention.

Experimental Study on the Vortex Flow in a Concentric Annulus with a Rotating Inner Cylinder

  • Kim, Young-Ju;Hwang, Young-Kyu
    • Journal of Mechanical Science and Technology
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    • v.17 no.4
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    • pp.562-570
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    • 2003
  • This experimental study concerns the characteristics of vortex flow in a concentric annulus with a diameter ratio of 0.52, whose outer cylinder is stationary and inner one is rotating. Pressure losses and skin friction coefficients have been measured for fully developed flows of water and of 0.4% aqueous solution of sodium carboxymethyl cellulose (CMC), respectively, when the inner cylinder rotates at the speed of 0~600 rpm. Also, the visualization of vortex flows has been performed to observe the unstable waves. The results of present study reveal the relation of the bulk flow Reynolds number Re and Rossby number Ro with respect to the skin friction coefficients. In somehow, they show the existence of flow instability mechanism. The effect of rotation on the skin friction coefficient is significantly dependent on the flow regime. The change of skin friction coefficient corresponding to the variation of rotating speed is large for the laminar flow regime, whereas it becomes smaller as Re increases for the transitional flow regime and. then, it gradually approach to zero for the turbulent flow regime. Consequently, the critical (bulk flow) Reynolds number Re$\_$c/ decreases as the rotational speed increases. Thus, the rotation of the inner cylinder promotes the onset of transition due to the excitation of Taylor vortices.

Isolation and its effect of a second organism for single cell protein(SCP) production (세균 단세포단백질(SCP) 생산을 위한 보조균주의 분리와 그 효과)

  • 권오진;양성호
    • Journal of Environmental Health Sciences
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    • v.22 no.2
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    • pp.10-18
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    • 1996
  • Experiments were carried out to find the possibility of an economic production of single cell protein(SCP) in mixed culture by Cellulomonas sp. KL-6 and a second organism. The second organism, strain LI-10, was isolated from the large intestines of a mouse. 1. When these strains were mixed, cell growth and carboxymethyl cellulase (CMCase) activity were increased to about 63% and 161%, respectively compared with that of single culture of strain KL-6. We found the mixed culture as a proper method of degradation of cellulose in our study. 2. Strain LI-10 was identified as E. coli. 3. This strain produced trace amounts of cellobiose, but glucose was not found in detectable amounts in the filter paper(FP) medium. 4. $CaCO_3$ injected in the medium at the ratio of 0.1% not only enhanced cell growth but also was effective as an acid neutralizing agent. 5. When this organism was cultured under the optimal medium (glucose 0.1%, $NH_4Cl$ 0.1%, yeast extract 2.0%, $KH_2PO_4$ 0.1%, KCl 0.05%, pH 7.2 and a temperature 30$\circ$C) for 5 days, a cell mass produced 1.18 g/l. The results showed the increase of cell mass up to 300% compared to 0.28 g/l produced in CMC medium.

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A Study on the Helical Flow of Newtonian and non-Newtonian fluid (뉴튼 및 비뉴튼 유체의 헬리컬 유동에 관한 연구)

  • Kim Young-Ju;Kim Chul-Soo;Hwang Young-Kyu
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.17 no.1
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    • pp.8-15
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    • 2005
  • This study concerns the characteristics of helical flow in a concentric and eccentric annulus with a diameter ratio of 0.52 and 0.9, whose outer cylinders are stationary and inner ones are rotating. Pressure losses and skin friction coefficients have been measured for fully developed flows of water and $0.2\%$ aqueous of sodium carboxymethyl cellulose(CMC), respectively, when the inner cylinder rotates at the speed of $0\~500$ rpm. The effect of rotation on the skin friction coefficient is significantly dependent on the flow regime. In all flow regimes, the skin friction coefficient is increased by the inner cylinder rotation. This study shows the change of skin friction coefficient and wall shear stress corresponding to the variation of rotating speed of the inner cylinder, radius ratio, eccentricity, and working fluids.