• Journal of Plant Biotechnology
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• v.30 no.4
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• pp.369-373
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• 2003

In spite of potential benefits of anther culture, low productivity of plant regeneration in some genotypes; e.g. tonsil and indica rice, is one of the major obstacles for practical use of anther culture. This study was conducted to improve cold shock method and carbohydrate source for increasing the efficiency of anther culture in rice. The most common carbon source, sucrose was replaced to maltose, which has two molecules of glucose. Maltose increased callus induction 1.4-to 1.8-fold higher in japonica rice, 3.2-to 11.6-fold in tongil types and 2.7-fold in indica rice IR50. Callus induction was increased from 0.2％ to 12.5％ in maltose medium compared to the medium supplemented with sucrose plus glucose in indica rice "Tetep". A simple procedure of vacuum packaging of panicles during cold shock treatment prolonged not only anther viability more than 15 days but also increased callus induction more than 2-fold compared to open-air storage (conventional method). Combining of above two methods, callus induction was increased 28 to 56％ in japonica, 13 to 33％ in tonsil type and 12 to 31％ in indica rice. Plant regeneration was increased 14 to 35％ in japonica, 10 to 20％ in tonsil and 4 to 15％ in indica rice, respectively.

• 한국신재생에너지학회:학술대회논문집
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• 2011.11a
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• pp.114.2-114.2
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• 2011

Furfural is a versatile derivative. It can be utilized for a building-block of furfuryl alcohol production and a component of fuels or liquid alkanes. But in bio-process, furfural is a critical compound because it inhibits cell growth and metabolism. Furfural could be converted from xylose and usually produced from biomass in which hemicellulose is abundant. In this study, furfural production from miscanthus was performed and utilization of miscanthus residue was consequently conducted. At first, hydrolysis for investigation of miscanthus composition and furfural production was performed using sulfuric acid. Previously, we optimized dilute acid pretreatment condition for miscanthus pretreatment and the condition was found to be about 15 min of reaction time, 1.5% of acid concentration and about $140^{\circ}C$ of temperature and 60% (about 7 g/L) of xylose was solubilized from miscanthus. Using the xylose, furfural production was conducted as second step. Approximately $160{\sim}200^{\circ}C$ of temperature was accompanied with the hydrolysis for pyrolysis of biomass. When the investigated condition; $180^{\circ}C$ of temperature, 20 min of reaction time and 2% of acid concentration was operated for furfural production, furfural productivity was reached to be 77% of theoretical maximum. After reaction, residue of miscanthus was utilized as feedstock of ethanol fermentation. Residue was well washed using water and saccharified using hydrolysis enzymes. Hydrolysate (glucose) from saccharification was utilized for the carbon source of Saccharomyces cervisiae K35.

• Journal of the Korean earth science society
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• v.34 no.7
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• pp.681-692
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• 2013

We aimed to evaluate the effectiveness of the Giggenbach bottle method and develop the related pretreatment and analytical methods using artificial fumarolic gases. The artificial fumarolic gases were generated by mixing $CO_2$, CO, $H_2S$, $SO_2$, $H_2$, and $CH_4$ gas streams with a $N_2$ stream sparged through an acidic medium containing HCl and HF, with their compositions varied by adjusting the gas flow rates. The resultant fumarolic gases were collected into an evacuated bottle partially filled with a NaOH absorption solution. While non-condensible gases such as CO, $H_2S$, and $CH_4$ accumulated in the headspace of the bottle, acidic components including $CO_2$, $SO_2$, HCl, and HF that were dissolved into the alkaline solution. Like other acidic components, $H_2S$ also dissolved into the solution, but it reacted with dissolved $Cd^{2+}$ to precipitate as CdS when $Cd(CH_3COO)_2$ was added. The non-condensible gases were analyzed on a gas chromatography. Then, CdS precipitates were separated from the alkaline solution by filtration, and they were pretreated with $H_2O_2$ to oxidize CdS-bound sulfide into sulfate. In addition, a portion of the solution was also pretreated with $H_2O_2$ to oxidize sulfite to sulfate. Following the pretreatment, the resultant samples were analyzed for $SO_4^{2-}$, $Cl^-$ and $F^-$ on an ion chromatography. In the meanwhile, dissolved $CO_2$ was analyzed on a total organic carbon-inorganic carbon analyzer without such pretreatment. According to our experimental results, the measured concentrations of the fumarolic gases were shown to be proportional to the gas flow rates, indicating that the Giggenbach bottle method is adequate for monitoring volcanic gas. The pretreatment and analytical methods employed in this study may also enhance the accuracy and reproducibility of the Giggenbach bottle method.

• Korean Journal of Food Science and Technology
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• v.37 no.5
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• pp.822-826
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• 2005

Effect of Cheonnyuncho extract on the liver injury of rats treated carbon tetrachloride $(CCl_4)$ was studied. Cheonnyuncho extract was administerd at dose of 0.5 and 1 g/kg/day, p.o. for 2 weeks. $CCl_4$ was treated at dose of $0.5\;mL/kg$, i.p. 3 hours later from the last pretreatment of Cheonnyuncho extract. Administration of Cheonnyuncho extract at a dose of 1 g/kg decreased serum AST, ALT and ALP activities by 36, 41, and 22% respectively compared to $CCl_4$ treatment group. Increased lipid peroxidation and decreased SOD and GST activities were also recovered by pretreatment of Chonnyuncho extract in liver of rats. These results suggest that Cheonnyuncho extract has hepatoprotective effect against liver injury.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.4
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• pp.857-864
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• 2009

This study aimed at developing the two stage and dual filtration system. It has a sand + activated carbon layer above the underdrain system and a sand layer above the middledrain system for pretreatment. When retrofitting an old filter bed or designing a new one, this technology can substitute the existing sand filter bed without requiring a new site. In order to extend the filtering duration, the upper layer of the filter bed consists of the rapid sand filtration with large particles which pre-treats and removes coarse particles and turbidity matters. The middle layer has biological activated carbon(BAC) and granular activated carbon(GAC) to eliminate dissolved organic matters, disinfection by-products precursors etc. The lower layer consists of the sand filtration for the post filtering mode. In this study, a pilot plant of two stage and dual filtration system was operated for 4 months in the S water treatment plant in Kyounggi-Do. The stability of turbidity was maintained below 1NTU. The TOC, THMFP and HAAFP were removed about 90% by two stage and dual filtration system, which is almost 2 times higher than S WTP. From analysis result of HPC along the depth of activated carbon + sand layer at 2nd stage, microorganism was mostly not detected, however, increment of HPC was shown as it becomes deeper. It indicates that growth of microorganism is occurred at activated carbon layer.

• Journal of Life Science
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• v.11 no.5
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• pp.405-414
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• 2001

Protaetia brevitarsis has been utilized as an ingredient of the description for the treatment of patients with chronic hepatic diseases in oriental medicine. This study was attempted to investigate whether Protaetia brevitarsis extract(PBE) protects or modulates the liver injuries induced by carbon tetrachloride or ethanol in Sprageue-Dawley rate. The liver injuries of rats induced by the treatment of carbon tetrachloride or ethanol were manifested by the observation of the significant changes in liver weight, serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities, serum thiobarbituric acid reactive substances (TBARS), and microsomal detocification enzymes(cytochrome P_450), cytochrome b$_{5}$, and cytochrome b$_{5}$ reductase).The effect of PBF on the liver damage induced by the chemicals was evaluated with the extent modulated in change of biochemical parameters above. Exposure to ethanol alone resulted a significant change in the ration of liver per body weight, ALT activity, and microsomal detoxification enzymes (cytochrome P_450, cytochrome b$_{5}$, and cytochrome b$_{5}$ reductase), but did not significantly changes in the levels of serum AST activity and TBARS. Pretreatment coith PBE did not modulate the alteration of the ratio of liver to body weigth, and the activities of serum aminotransferascs (AST. ALT), TBARS, and micro somal detoxification enzyme (cytochrome p_450, cytochrome b$_{5}$,and cytochrome b$_{5}$ reductase. These result suggested that PBE has not appreciable therapeutic effect on carbon tetrachloride or ethanol induced hepatotoxicity.

• Applied Chemistry for Engineering
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• v.32 no.6
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• pp.640-646
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• 2021

In this study, pitch was synthesized using pyrolysis fuel oil (PFO). Coke with mesophase microstructure was then prepared from the synthesized pitch and its properties were evaluated. Pitch was synthesized by poly-condensation reaction, which is an endothermic reaction at a temperature above 400 ℃ because the PFO was mainly composed of molecules with two to three aromatic rings. The Coke reactor was composed of the pretreatment reactor, preheater for applying heat energy, and coke drum for inducing microstructure of coke. Coke was prepared from synthesized pitch by controlling the temperature of the preheater to 400~490 ℃, and properties were evaluated by polarization microscope, XRD and Raman spectroscopy. The coke prepared at a preheater temperature of 460 ℃ identified flow anisotropic microstructure, and the electrical conductivity was 72.0 S/cm due to high crystallinity. And the flow anisotropic coke showed approximately 2.2 times higher electrical conductivity than that of Super-P, a conductive carbon material.

• Journal of the Korean Wood Science and Technology
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• v.43 no.3
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• pp.344-354
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• 2015

To investigate the effects of ionic liquid pretreatment on biomass, giant miscanthus was treated with 1-ethyl-3-methylimidazolium acetate ([Emim][OAc]) and 1-butyl-3-methylimidazolium acetate ([Bmim][OAc]) at three temperature conditions ($90^{\circ}C$, $110^{\circ}C$, and $130^{\circ}C$). As temperature condition increased, yield of the cellulose-rich product (CP) was reduced from 87.2% to 67.6%, while yield of the ionic liquid lignin (ILL) increased from 2.2% to 9.9%. Compared to the ILL, CP had lower carbon contents and higher oxygen contents. Enzymatic hydrolysis of CPs showed that conversion ratio of CP treated with [Emim][OAc] at $110^{\circ}C$ was 56.7%, the highest digestibility. Thermogravimetric analysis indicated that the maximum degradation rate decreased as temperature condition increased. In addition, maximum degradation temperature of ILL treated with [Emim][OAc] ranged from 274 to $279^{\circ}C$ which was lower than that of ILL treated with [Bmim][OAc]. Analytical date for ${\beta}$-O-4 linkage frequency in the ILL revealed that ${\beta}$-O-4 linkage frequency in the ILL decreased as the temperature rose. Furthermore, the highest S/G ratio of the ILL was determined to ca. 1.2 obtained from [Bmim][OAc] treatment at $110^{\circ}C$.

• Journal of Soil and Groundwater Environment
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• v.9 no.1
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• pp.87-94
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• 2004

The effects of pretreatment of GAC and dissolved oxygen (DO) on the sorption capacity for ο-cresol were investigated using pretreated GAC under oxic and anoxic conditions. Virgin GAC was used with pretreated GACs by $O_2$, acid and base as sorbents. Sorption capacity of GAC was dependent on the oxygen conditions according to the pretreatment methods. Virgin GAC showed increased sorption capacity when DO was present in the solution, while $O_2$-pretreated GAC did not show any considerable capacity change. Acid- and base-pretreated GACs were relatively more influenced by presence of DO as compared with virgin GAC. Both acid and base-pretreated GACs showed a rapid sorption rate at the initial stage, but as contact time became longer the sorption was slower. Sorbed ο-cresol was extracted with micro-Soxhlet extraction apparatus using the GAC separated from the rate experiments. Within 1 hour both acid and base-pretreated GACs showed the decrease in extraction efficiencies under both oxic and anoxic conditions. After 1 hour such a trend (the increase as contact time was longer) was not observed and showed relatively constant efficiencies of 35∼50%. According to the results of this study $O_2$contacted with GAC before sorption as well as DO present in the solution during sorption could influence the GAC sorption capacity.

• Food Science and Preservation
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• v.16 no.5
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• pp.623-628
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• 2009

Fresh ginger rhizomes were heat-treated for 10 min, 30 min, or 60 min using hot air ($40^{\circ}C$, $50^{\circ}C$, or $60^{\circ}C$), and stored in low-density polyethylene (0.04 mm thickness) bags for 2 months at $12{\pm}1^{\circ}C$. We studied the effects of heat pretreatment on changes in gas levels after packaging, and quality characteristics of the rhizomes. Oxygen and carbon dioxide levels progressively fell and rose, respectively, as the temperature of heat treatment rose and the duration of such treatment was extended. The sprouting rate of ginger rhizomes treated at $40^{\circ}C$ was higher than that of other samples. Rotting, softening, and increasing pH of rhizomes were accelerated by treatment at higher temperature for a longer time. Weight loss and soluble solid levels were not affected by heat treatment. Sensory qualities such as appearance, odor, and overall acceptability of rhizomes fell with treatment at a higher temperature for a longer time. These results suggest that heat pretreatment has a detrimental effect on the quality of fresh ginger rhizomes.