• Korean Journal of Food Science and Technology
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• v.44 no.6
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• pp.750-758
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• 2012

This study investigated flavonoid, total phenol, total flavonoid content, antioxidant and antiproliferative activity on human breast cancer cells (MCF-7, MDA-MB-231). Four varieties of Korean green peppers (KP: kkuri pepper, PP: phut pepper, CP: cheongyang pepper, OP: ohi pepper) and one foreign green pepper (JP: jalapeno) were used. The contents of luteolin, quercetin and apigenin, which are abundant flavonoids in green pepper, were the highest in KP. Also, the contents of total phenol, and total flavonoids were the highest in KP, followed by CP, JP, PP, and OP (KP: total phenol $13.29{\pm}0.45$ mg GAE/g D.W., total flavonoid $7.02{\pm}0.13$ mg QE/g D.W. In DPPH ABTS radical-scavenging activity, KP showed the most potent antioxidant activity. In the result of viability in human breast cancer cells, KP had the highest antiproliferative effect. These results suggest that green peppers have significant antioxidant activity and can be a possible candidate for treatment of breast cancer.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.3
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• pp.339-343
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• 2012

This study was performed to examine the effect of the clay mineral illite on the improvement of soil and plant growth. Red pepper (Capsicum annuum L.) was used as a test vegetable crop. The experiment was performed during six weeks in the plantation of the Chungbuk National University. Its seedlings were cultivated in the soil normally used for horticultural purpose. Among the seedlings germinated the healthy and regular size of seed were selected and cultivated in the plantation. They were treated with two forms of illite, particulate (PA) and powder (PW), at the following application rates: standard application[P1 (PA1, PW1), soil: illite = 1:20 (w/w)] and two times [P2 (PA2, PW2), 1:10 (w/w)] of standard application. Untreatment (P0) was used as a control soil. At six weeks of cultivation, their growth lengths were correspondingly increased as the application rate was increased ranging from P0, P1, and P2. Their growth length was a little greater with the application of powder illite (PW) than with the particulate illite (PA). Based on the plant analysis of root, leaf, and stem of red pepper, the uptake amounts of K, Ca, and Mg, were correspondingly increased, as the application rate was increased ranging from P0, P1, and P2 respectively. At the same application rate, their amounts taken up in the respective parts were higher with the application of PW illite than on the PA one. Especially the amounts of Ca and Mg were higher in the stem and leaf than root. Consequently, it appears that the illite treatment, especially, PW form of illite, enhance the growth of red pepper in the plantation during the six weeks of experiment.

• Horticultural Science & Technology
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• v.30 no.1
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• pp.64-72
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• 2012

Phytophthora root rot has been causing a serious yield loss in pepper production. Since 2004, the year in which commercial cultivars resistant to the disease were firstly commercialized, it has been necessary to introduce the resistance into domestic pepper cultivars for dried red pepper. Therefore, developing molecular markers linked to the resistance is required for an accurate selection of resistant plants and increasing breeding efficiency. Until now, several markers associated with the major dominant gene resistant to Phytophthora root rot have been reported but they have some serious limitations for their usage. In this study, we aimed to develop molecular markers linked to the major dominant gene that can be used for almost of all genetic resources resistant to Phytophthora root rot. Two segregating $F_2$ populations derived from a 'Subicho' ${\times}$ 'CM334' combination and a commercial cultivar 'Dokyacheongcheong' were used to develop molecular markers associated with the resistance. After screening 1,024 AFLP primer combinations with bulked segregant analysis, three AFLP (AFLP1, AFLP2, and AFLP3) markers were identified and converted into three CAPS markers (M1-CAPS, M2-CAPS, and M3-CAPS), respectively. Among them, M3-CAPS marker was further studied in ten resistants, fourteen susceptibles, five hybrids and 53 commercial cultivars. As a result, M3-CAPS marker was more fitted to identify Phytophthora resistance than previously reported P5-SNAP and Phyto5.2-SCAR markers. The result indicated that the M3-CAPS marker will be useful for resistance breeding to Phytophthora root rot in chili pepper.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.65 no.2
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• pp.130-141
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• 2020

The purpose of this study was to determine which fermented liquid fertilizer and application method yields the greatest amount of growth in red pepper (Capsicum annuum L.) and tomato (Lycopersicon esculentum MILL.) plants. Additionally, we investigated which extraction methods produce the most effective fertilizer with the highest levels of mineral nutrients. The liquid fertilizers used in this study were made from fish, bone and fish meal, red pepper leaves, and oil cake, and were extracted using fermentation or water and boiled water. In tomato plants, foliar-application of fermented fertilizer is known to promote more growth than application by drenching, regardless of the number of treatments (once or twice). In our studies, however, drenching with fertilizer promoted growth more effectively than foliar-application in red pepper plants. Studies in both tomato and red pepper have shown that the number of treatments does not significantly alter growth. Liquid fertilizers produced by a fermentation-extraction method promoted greater levels of growth in tomato compared to red pepper, and growth was greater when fertilizers were applied 20 (rather than 40) days post-sowing. Red pepper and tomato shoot fresh weight were affected more by fermented fertilizers than plant height 20 days post-sowing. In red pepper, we observed increased shoot fresh weight when using fermented liquid fertilizers with concentrations of 0.1% or greater. Tomato shoot fresh weight increased similarly in response to fermented fertilizer treatments at the same concentration levels, except those derived from fish. Fermented fish liquid fertilizer was only effective in increasing tomato shoot fresh weight in concentrations exceeding 1%. Red pepper and tomato shoot fresh weight also increased more than plant height in our studies using fermentation liquid fertilizers at 40 days after sowing. Red pepper fresh weight increased with application of bone + fish meal, red pepper leaf, and oil cake fertilizers at concentrations of 0.1%, but not with fish liquid fertilizer in concentrations under 0.5%. Shoot fresh weight in tomato increased with all liquid fertilizers. Growth in red pepper and tomato may be influenced by different kinds of fertilizers due to combinations of macro- and micro-nutrients, or specific macro-nutrients such as nitrogen, phosphoric acid, and potassium. The mineral nutrients found in fish, bone and fish meal, red pepper leaves, and oil cake were not easily extracted by fermentation; thus, liquid fertilizers made using water and boiled water methods more effectively promoted growth in red pepper and tomato due to the larger amounts of macronutrients eluted.

• Korean Journal of Environmental Agriculture
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• v.24 no.2
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• pp.191-197
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• 2005

Green house soils have been intensively cultivated with excessive application of compost and chemical fertilizer for vegetable growth. The objective of this study was to establish the reasonable fertilizer application system for rice cultivation in green house soil. Field experiment was carried out with rice cv. Geumo-byeo 1 in Jisan series soil (fine loamy, mixed, mesic family of Fluventic Haplaquepts) that was previously cropped with green pepper (Capsicum annuum L.) for the last 3 years. Treatment consisted of conventional fertilization $(N-P_2O_5-K_2O=11-4.5-5.7kg\;10a^{-1})$, no basal fertilization, 50% reduction of basal fertilization no top dressing, bulk blending fertilizer, and no fertilizer. The value of pH, available phosphate, and exchangeable potassium after experiment was lower than those before experiment while organic matter content was not difference in all treatment. The value of salt elusion was the highest in no basal fertilization plot. The amount of $NH_4-N$ in soil was higher in growth stage of rice as fertilizer amount increased in 1998. The changes of plant height and tiller were higher as fertilizer amount increased. Thousand-grain weight as yield component was higher in no basal fertilization plot all the year because of decreasing panicle. There was no significant difference in rice yield between treatments in 1998. However, conventional fertilization resulted in significantly increased rice yield in 1999. Nitrogen use efficiency was the highest in no basal fertilization plot in 1998 and in conventional fertilization plot in 1998. Our results suggest that no basal fertilization be best to increase salt elusion with slightly increased yield in first year for rice cropping after vegetable harvesting, which method improves fertilization efficiency. However, conventional fertilization was good for second rice cropping after vegetable harvesting in greenhouse.

• Journal of Bio-Environment Control
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• v.18 no.3
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• pp.238-243
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• 2009

To investigate the characteristics of moisture content (MC), moisture distribution and starting point of drainage in a rockwool slab culture, time domain reflectometry (TDR) sensors were used in a drip irrigation system. MC values ($0{\sim}100%$) measured by TDR sensors in a slab were compared to those by loadcells. Seventy two seedlings of paprika (Capsicum annuum L.) were cultured for $5{\sim}6$ months in a green-house and the starting point of irrigation was determined by the average value of three TDR sensors which were inserted diagonally across the slabs under the plants. MCs as a standard for starting point of irrigation by TDR were determined with 40%, 50%, and 60%. Distribution of MCs in a slab measured with five TDR sensors equally spaced from two irrigation points were not much different when the MC in the slab increased from zero to saturation point. The saturated MCs in the slab were presented at $58{\sim}65%$ and the drain was started when the MC became around $50{\sim}55%$. At the saturated MC in the slab, TDR sensors presented 100% but the values from the loadcell showed 90% at the same time. However, measurement errors between two methods for MC remarkably decreased with a decrease in the MC in a slab. Especially when the MC was maintaining below 60%, the errors between TDR and loadcell methods for measuring MC in the rock-wool slab were less than 5%. There were no significant differences in number of fruits and fresh and dry weights of fruits when they were cultured under the different MC conditions with three irrigation regimes (40%, 50%, and 60%). These results indicated that the MC control by TDR sensors in a rock-wool based paprika culture can be suggested as a method to determine the starting point of irrigation for a soilless culture system.

• Journal of Bio-Environment Control
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• v.23 no.2
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• pp.139-143
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• 2014

This study was conducted to investigate the effect of cultivation period and rockwool block size on the growth and early yield of paprika transplants grown in a closed transplant production system. Paprika seeds were sown and germinated in three different size of rockwool blocks ($45{\times}40{\times}35$, $70{\times}70{\times}60$, $100{\times}100{\times}65mm$) and cultivated in a closed transplant production system for 23, 30, and 37 days after sowing. Paprika transplants were cultivated using $100{\times}100{\times}65mm$ rockwool blocks in a greenhouse following a conventional and typical production method for comparing with the growth of paprika transplants grown in a closed transplant production system. Also, we transplanted paprika transplants grown for 23, 30, and 37 days in a closed transplant production system and greenhouse to rockwool slabs and investigated fresh weight of fruits and yield 125 days after sowing. The growth of paprika transplants grown in $70{\times}70{\times}60mm$ rockwool blocks in a closed transplant production system was highest, and the quality of paprika transplant grown in a closed transplant production system was better than in a greenhouse. Rockwool block size and cultivation period in a closed transplant production system did not affect fresh weight of fruits, however, yield was largest when paprika transplants were cultivated using $70{\times}70{\times}60$ and $100{\times}100{\times}65mm$ rockwool blocks for 23 days in a closed transplant production system. These results suggest that decreasing rockwool block size and cultivation period can be strategically used to enhance transplant quality and yield of paprika, as paprika transplants were cultivated in a closed transplant production system.

• Journal of Bio-Environment Control
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• v.26 no.4
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• pp.368-377
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• 2017

This study investigated the possibility of application of the recently introduced cylindrical paper pot seedlings in hot pepper (Capsicum annuum L.) cultivation. The seedling growth, initial rooting after planting and accumulated fruit yield were investigated with the treatments of tray type (paper pot and plug) as a main factor, tray cell number (40 cell and 50 cell) as a sub-factor, and fertigation method (continuous fertigation and fertigation after 35 days sowing) as a sub-sub factor, respectively. The growth of pepper seedlings was significantly affected by tray type and fertigation method showing the highest value at 50 cell plug tray with continuous fertigation, and the effect of fertigation was greater than that of trays. 'Cheongyang', 'Daekwonseoneon' and 'Longgreenmat' cultivar showed all the same pattern in seedling growth. These three-cultivar seedlings were planted in plastic house and in open field in Jeonju area, respectively, and another 'Daekwonseoneon' seedlings raised Yeongyang local area was also planted at the same area. There was no difference in the rooting of 'Cheongyang' pepper at 2 weeks after planting in plastic house. The accumulated fruit weight was not significantly different between paper pot seedlings and plug seedlings in three cultivar grown in plastic house. However, that of 'Cheongyang' pepper showed higher at paper pot seedlings than plug seedlings and the other two cultivar were higher at plug seedlings in open field. 'Daekwonseoneon' pepper yield grown in open field in 'Yeongyang' area was not significant between paper pot seedlings and plug seedlings. In conclusion, the pattern of seedlings growth grown in the cylindrical paper pot was the same as those of the conventional plug seedlings and also fruit yield was similar between paper pot seedlings and plug seedlings even though minute difference among cultivars. These results suggest that pepper seedlings grown in paper pot should be highly applicable to pepper cultivation.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.6
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• pp.759-765
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• 2007

This study was conducted to analyze the chemical properties of single.harvested pepper (YW211, YW213), and to investigate the change of kochujang quality by the addition of single-harvested peppers. Capsaicinoids content of YW211 was $271.65{\pm}25.10mg/100g$ and ASTA color value of YW213 was $212.71{\pm}2.38$, which were comparatively higher values than commercial red peppers. Mixed red pepper powder used for kochujang production were prepared with various mixing ratios of commercial red peppers (YY & GR) and single-harvested pepper (YW211 & YW213). Capsaicinoids content of YY, $3.98{\pm}0.24mg/100g$, was increased to $52.61{\pm}8.62mg/100g$ by mixing 30% YW211. ASTA color value of GR was $110.63{\pm}1.89$ and was increased to $130.01{\pm}1.31$ by mixing 30% YW213. The pH values of kochujang added YW211 or YW213 were slowly reduced and acidities were increased during fermentation; however, both values weren't statistically different. The contents of amino nitrogen increased until 60 days, and then decreased thereafter. Reducing sugars were increased considerably until 30 days, and reduced slowly after fermentation for 90 days.

• Journal of Plant Biotechnology
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• v.32 no.3
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• pp.167-173
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• 2005

Three different cDNAS for cinnamate 4-hydroxylase (C4H) which are involved in the second step of the general phenylpropanoid pathway were isolated and designated as pc4h1 (1,755 bp), pc4h2 (1,655 bp), and pc4h3 (1,316 bp), respectively. The nucleotide sequence analysis revealed that both pc4h1 and pc4h2 clones encode polypeptides of 505 amino acids frame but pc4h3 clone was truncated at the 5'-end of coding region. The alignment of the deduced amino acid sequences showed that PC4H1 and PC4H2 are highly homologous (95.8% identical) with each other and contain three conserved domains which are typical in cytochrome P450 monooxygenase: proline-rich region, threonine-containing binding pocket for the oxygen molecule, and heme binding region. In addition, result of the phylogenic tree analysis revealed that both pepper C4Hs belong to Class 1. pc4h2 transcription was strongly induced in wounded fruit (400%) and root (200%) relative to its very low basal level but not in leaf or stem tissue. In case of pc4h1, the basal level of transcription was higher than pc4h2 but induction by wounding was lower in fruit and root while leaf and stem tissues did not respond to wounding. The basal level of pc4h3 transcripts was not, if any, detectable and response to wounding was not observed.