• 한국식품영양과학회지
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• 제41권6호
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• pp.727-732
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• 2012

고추(Capsicum annuum)의 항산화 효과 및 생리활성 측정을 통하여 기능성식품의 소재로 활용하기 위한 기초 연구를 진행하였다. 폴리페놀 함량은 과육보다는 씨에서 높게 나타났다. 그러나 플라보노이드 함량은 풋고추 과육에서 높은 함량을 보였다. DPPH radical 소거활성에서 $SC_{50}$값은 홍고추 씨 $1,935{\pm}162\;{\mu}g\;DM/mL$, ABTS radical 소거활성에서는 $SC_{50}$값이 풋고추 씨 $3,354{\pm}76\;{\mu}g\;DM/mL$로 과육에 비해 높았다. 최종당화산물(AGEs) 생성 억제활성은 풋고추와 홍고추 씨, 풋고추와 홍고추 과육 순으로, ${\alpha}$-glucosidase 저해활성은 풋고추 과육, 홍고추 씨, 홍고추 과육, 풋고추 씨 순이었다. Tyrosinase 억제활성은 홍고추와 풋고추 씨, 홍고추와 풋고추 과육 순이었다. 종합해보면 기능성식품 소재로서 홍고추 씨 및 풋고추 씨가 과육보다 활용도가 높은 것으로 결론지을 수 있다.

• Journal of Plant Biotechnology
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• 제5권4호
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• pp.245-250
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• 2003

An efficient procedure was developed for adventitious shoot bud induction and plantlet regeneration from various explants of the ten genotypes of Pepper (Capsicum annuum L.) using Thidiazuron (TDZ). Among various treatments at 1.0-3.0 mg/L TDZ Induced maximum number of adventitious shoots depending upon the explant type and genotype compared to other treatments. Among the explants tested, leaf induced maximum number of adventitious shoots than the cotyledons. TDZ-mediated organo-genesis was possible in 10 pepper cultivars, the extent of the response being genotype-dependent. Of the ten genotypes tested, C. annuum cvs CA960, $G_4$ and X-235 were produced maximum number of adventitious shoots and Sell was the least, and all other genotypes gave moderate response. Elongation of multiple shoots was observed on medium supplemented with SA (0.05 mg/L) in combination of IAA (0.05 mg/L). Differences in ability for in vitro shoot regeneration and elongation depend upon the variety and explant type. The elongated shoots were success. Fully rooted on MS medium containing at 1.0 mG/L IAA. Plantlets regenerated from different explants of ten genotypes were found to be diploid (2n=24) and were devoid of any chromosomal aberrations. Regenerated plants were successfully established in soil where 85-90% of them developed into morphologically normal and fertile plants.

• 한국자원식물학회지
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• 제36권5호
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• pp.527-539
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• 2023

고추(Capsicum annuum L.)는 전 세계적으로 재배되고 있는 채소이자 향신료 작물이다. 고추 열매는 독특한 맛과 향을 지니면서 인체 건강에 중요한 항산화제와 다양한 화합물을 제공하기에 경제적 가치가 높다. 또한 소비자의 기호에 따른 새로운 고추 품종에 대한 수요도 높다. 하지만 고추는 조직 및 기관 분화와 식물 재분화가 어려운 식물이기에 유전자재조합 및 유전자 교정과 같은 새로 개발된 기술을 사용하여 요구되는 품종 개발이 어려운 상황이다. 본 연구에서는 서울대학교 Core collection에서 분양 받은 7계통의 고추를 이용하여 조직 배양 및 재분화 조건을 조사하였다. 여기에는 서로 다른 사이토키닌과 옥신 농도로 구성된 여러 가지 배지 조성을 사용하여 캘러스와 부정아 유도, 그리고 신초 형성을 관찰하였다. 그 결과, 호르몬 조성에 따라 캘러스 유도 및 신초 형성에서 품종별 차이가 있는 것으로 확인하였으며, zeatin riboside를 함유한 배지와 유묘의 잎자루 부분을 사용한 경우 재분화율이 가장 높게 나타났다. 특히 사용된 7 계통의 고추 중에서 CMV980 계통이 다른 품종에 비해 월등히 높은 재분화 효율(약48%)을 나타냈고, Yuwolcho 계통이 그 뒤를 이었다. 따라서 본 연구는 CMV980 및 Yuwolcho 계통을 고추 형질전환에 활용될 수 있는 우수한 품종 후보로 제공하며, 이는 향후 유전자교정기술 등을 이용한 다양한 품종 개발에 기여할 수 있을 것이다.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2005년도 2005 Annual Meeting & International Symposium
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• pp.272-272
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• 2005

• 한국동물학회:학술대회논문집
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• 한국동물학회 1995년도 한국생물과학협회 학술발표대회
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• pp.235.2-235
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• 1995

• 한국동물학회:학술대회논문집
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• 한국동물학회 1995년도 한국생물과학협회 학술발표대회
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• pp.236.2-236
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• 1995

• Molecules and Cells
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• 제25권1호
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• pp.20-29
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• 2008

Cytoplasmic male sterility (CMS), one of the most important traits in crop breeding, has been used for commercial seed production by $F_1$ hybrid cultivars of pepper (Capsicum annuum L.). To develop reliable molecular markers for allelic selection of the Restorer-of-fertility (Rf) gene, which is known to be a major determinant of pollen fertility restoration in peppers, a sequence of approximately 10 kb flanking an RAPD fragment closely linked to the Rf locus was obtained by genome walking. A homology search revealed that this sequence contained an LTR retrotransposon and a non-LTR LINE-like retrotransposon. Sequencing of this Rf-linked region to search for polymorphisms between a dominant and recessive allele revealed 98% nucleotide sequence identity between them. A third polymorphic haplotype of the Rf-linked sequence, which has 94-96% nucleotide sequence identity with the two previously isolated haplotypes, was identified among a large number of breeding lines. Utilizing polymorphic sequences in the haplotypes, PCR markers were developed for selection of particular haplotypes and used to examine the distribution of the haplotypes in diverse breeding lines, cultivars, and C. annuum germplasms. Surprisingly, the third haplotype was the predominant type in C. annuum germplasms, while its frequency in $F_1$ hybrid cultivars was relatively low. Meanwhile, analysis of breeding lines whose Rf allele genotypes and male-sterility phenotypes were already known revealed that the third haplotype was mainly present in exotic breeding lines that cause unstable male-sterility when combined with sterile cytoplasms.

• 생약학회지
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• 제48권2호
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• pp.160-165
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• 2017

Capsicum annuum L. (CA) is reported to be the most widely cultivated species of the Capsicum throughout the world. Its fruit and the seeds have been used as spice after drying and grinding. It is reported that the fruit and seeds contain capsaicinoid such as capsaicin and dihydrocapsaicin, carotenoid such as capsanthin and ${\beta}$-carotene with its anti-tumor effect, anti-obesity effect, anti-diabetic action. Present study, we isolated two lignans, icariside $E_5$ and vanilloyl icariside $E_5$ from the seed of CA and validation and content determination were confirmed using High-Performance Liquid Chromatography (HPLC). As a result, the contents of the lignans, icariside $E_5$ and vanilloyl icariside $E_5$ were 0.28%, 0.085%, respectively in the extract.

• The Plant Pathology Journal
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• 제25권2호
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• pp.113-120
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• 2009

Microscopic study of chili pepper (Capsicum annuum L.) infected with Phytophthora capsici, causing Phytophthora blight of chili pepper, was conducted to compare histological and cytological characteristics in the root and stem of susceptible (C. annuum cv. Bugang) and resistant (C. annuum cv. CM334) pepper cultivars. The susceptible pepper roots and stems were extensively penetrated and invaded by the pathogen initially into epidermal cells and later cortical and vascular cells. Host cell walls adjacent to and invaded by the infecting hyphae were partially dissolved and structurally loosened with fine fibrillar materials probably by cell wall-degrading enzymes of the pathogen. In the resistant pepper, the pathogen remained on root epidermal surface at one day after inoculation, embedded and captured in root exudation materials composed of proteins and polysaccharides. Also the pathogen appeared to be blocked in its progression at the early infection stages by thickened middle lamellae. At 3 days after inoculation, the oomycete hyphae were still confined to epidermal cells of the root and at most outer peripheral cortical cells of the stem, resulting from their invasion blocked by wound periderms formed underneath the infection sites and/or cell wall appositions bounding the hyphal protrusions. All of these aspects suggest that limitation of disease development in the resistant pepper may be due to the inhibition of the pathogen penetration, infection, invasion, and colonization by the defense structures such as root exudation materials, thickened middle lamellae, wound peridems and cell wall appositions.

• 한국식품영양학회지
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• 제27권4호
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• pp.678-683
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• 2014

This study was conducted to investigate the effect of Korean red pepper extracts on physiological and sensory characteristics. The Korean red pepper extracts were added to chocolate at weight percentages of 0, 2, 4, and 6%. The sensory characteristics made with various Korean red pepper concentrations of the additives were measured as follows: color values (L-value, redness, and yellowness), total phenol, total capsaicinoid, total carotenoid, and ABTS radical scavenging activity. In sensory evaluation, significant differences (p<0.05 and p<0.01) were shown in taste, spiciness, and overall acceptability depending on the addition of pepper extracts. However, there were no significant differences in the properties of aroma and bitterness for chocolate.