• 분석과학
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• 제19권1호
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• pp.31-38
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• 2006

Ion-Paring을 이용한 고성능 액체 크로마토그래피법과 계면활성제를 완충용액에 섞어서 사용하는 미셀 모세관 전기영동법(micellar electrokinetic capillary chromatography, MECC)을 이용하여 아조염료의 합성성분이면서 동시에 독성을 나타내는 분해물인 H-acid, J-acid, ${\gamma}$-acid, orthanilic acid, sulfanilic acid 그리고 2-naphthylamine-1,5-disulfonic acid 등의 디아조 성분에 대하여 분석을 수행하였다. 같은 방법으로 Acid Orange 7, Acid Orange 5, Acid Blue 92 등의 산성염료와 Direct Red 80 등의 직접염료와 같은 반응성 염료 및 Calcion에 대한 분리를 시도한 결과 모든 염료에 대한 완전한 분리를 얻었으며, 특히 각 염료의 환원용액을 H-acid, J-acid, ${\gamma}$-acid, orthanilic acid, sulfanilic acid 혹은 2-naphthylamine-1,5-disulfonic acid 등의 표준물질과 비교 분석한 결과 사용한 각 염료의 디아조 혹은 커플링 성분을 완벽하게 분석할 수 있음을 알 수 있었고, 따라서 Ion-Pair 크로마토그래피법과 모세관 전기영동법은 미지의 염료에 대한 성분확인 및 디아조 혹은 커플링 성분분석에 응용할 수 있음을 보였다.

• 한국식품과학회지
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• 제44권5호
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• pp.503-525
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• 2012

Consumers are increasingly concerned about the origin of foods, so the geographical origin of foods has been a major topic of debate and extensive research. Various instrumental methods (e.g. high performance liquid chromatography (HPLC), gas chromatography (GC), capillary electrophoresis (CE), electronic nose, near-infrared spectroscopy (NIRS), nuclear magnetic resonance spectroscopy (NMR), DNA analysis, multi-isotope analysis) in conjunction with statistical analysis, were developed and applied in attempt to provide reliable answers to their geographical origin. This study reviews current developments in the application of various methods for a clear geographical origin of foods. The limitation of discrimination analysis for geographical origin was also discussed.

• 한국정밀공학회지
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• 제24권1호
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• pp.47-56
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• 2007

Recently, demands on mechanical micro machining technology have been increased in manufacturing of micro-scale precision shapes and parts. The main purpose of this research is to verify the accuracy and cost efficiency of the mechanical micro machining. In order to measure the precision and feasibility of mechanical micro machining, various micro features were machined. Aluminum molds were machined by a 3-axis micro stage in order to fabricate microchips with $200{\mu}m$ wide channel for capillary electrophoresis, then the same geometry of microchip was made by injection molding. To evaluate the cost efficiency of various micro manufacturing processes, cost estimation for mechanical micro machining was conducted, and actual costs of microchips fabricated by mechanical micro machining, injection molding, and MEMS (Micro electro mechanical system) were compared.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2011년도 제42회 하계학술대회
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• pp.1688-1689
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• 2011

A simple and rapid capillary electrophoresis method was developed for the quantitative analysis of common triazine herbicides. Cyclic voltammetry was employed to clarify the detection voltage which showed characteristic irreversible cathodic peaks. For the analysis, the mixture of triazine herbicides was applied in a microfluidic chip to determine the CE-separated peaks. Soil sample extracts were analyzed directly after drying and redissolution with the supporting electrolyte but without other pretreatment. The results were comparable to those obtained by HPLC with UV detection. Therefore, this method can be used in the rapid determination of pesticide/herbicide residues.

• 소성∙가공
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• 제15권9호
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• pp.692-696
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• 2006

Micro fabrication of polymeric materials becomes increasingly important. And it is considered as a low-cost alternative to the silicon or glass-based Micro Electro-Mechanical System(MEMS) technologies. In the present study, micro channels were fabricated via LiGA(Lithographie, Galvanoformung, Abformung) process used for Capillary Electrophoresis(CE) chip. Taguchi method was applied to investigate the effects of process conditions in injection molding(melt temperature, injection speed, mold temperature and packing pressure) on the transcription characteristics of the micro channel. It was found that the skin layer disturbs a formation of micro channel. Furthermore, mold temperature and injection speed were two important factors to affect the replication characteristics of micro channel.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2008년도 제39회 하계학술대회
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• pp.1460-1461
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• 2008

마이크로칩 형태에서의 모세관 전기영동과 전류법을 이용하여 디옥시리보핵산(DNA) 단편들의 분리 검출하는 실험을 하였다. 마이크로 채널이 형성된 PDMS(polydimethylsiloxane)와 금 전극이 형성된 유리 기판을 접합하여 마이크로칩을 제작하였다. 20V/cm의 전계를 인가하여 100bp-1.5kbp 길이의 DNA 단편을 모세관 전기영동 하였을 때 250초내에 분리 검출되는 것을 확인하였다.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2012년도 한국컴퓨터종합학술대회논문집 Vol.39 No.1(B)
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• pp.453-455
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• 2012

생물 검정(Bioassay)이란 생체 조직이나 분자의 구조 분석이나 기능 해석, 화합물이나 약에 의한 영향성을 실험하기 위해 실험체 조직과 약물의 상호작용에 의한 생성물의 양적 세기를 측정하는 과학적 실험 방법의 총칭이다. 바이오 어세이 실험 방법은 Gas Chromatography, 시험관 전기영동(Capillary Electrophoresis), 핵자기공명(NMR) 등의 다양한 실험 데이터를 포함한다. 결과로 생성된 실험 데이터를 정량적으로 분석하기 위해서는 일관성을 위해 얻어진 데이터를 정렬하는(alignment) 과정을 거쳐야 한다. 본 연구에서는 알려진 정렬 알고리즘들을 비교하기 위해, 알고리즘의 유형별로 분류하고 그 결과물을 분석하여 성능을 비교함과 동시에 특성을 파악하고자 한다.

• 대한전기학회논문지:전기물성ㆍ응용부문C
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• 제54권10호
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• pp.449-452
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• 2005

We fabricated an electrochemical detector (ECD) to catalyze redox reaction efficiently by electrodepositing Prussian blue (PB) on the indium tin oxide (ITO) electrode. Capillary electrophoresis (CE) and amperometric method were used. We investigated the PB surface properties by topography from atomic force microscopy (AFM). Also PB film thickness calibration with respect to deposition time and voltage was used to get better PB surFace. The PB thin film of dense and smooth surface could catalyze redox reaction efficiently. Comparing with CE-ECD microchip using bare-lTO electrode, proposed CE-ECD microchip using PB deposited electrode has shown better sensitivity by determining the detected peak current from the electropherograms while the concentration of tested analyzes was maintained the same. It is verified that detection limit can be lowered for 0.01 mM of dopamine and catechol respectively.

• 생명과학회지
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• 제10권2호
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• pp.140-149
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• 2000

In order to utilize marine processing waste which would normally be discarded, cod liver protein was hydrolysed by ${\alpha}$-chymotrysin, and the hydrolysate was investigated for the new angiotensin I converting enzyme (ACE) inhibitor. Thy hydrolysate was separated into three major types, with molecular weight cut-off (MWCO) values less than 10 kDa, 5 kDa and 1 kDa of ultrafiltration membranes, respectively. ACE inhibitory peptides were isolated from the fractions passed through MWCO 1 kDa membrane, and purified by using ion-exchange chromatography on a SP-Sephadex C-25 column, gel filtration on a Sephadex G-15 column, and HPLC on an ODS column. The purity was identified with capillary electrophoresis. The amino acid sequences of two peptides were Met-Ile-Pro-Pro-Tyr-Tyr (IC50=10.9 ${\mu}$M) and Gly-Leu-Arg-Asn-Gly-Ile (IC50=35.0 ${\mu}$M)

• 한국식품영양과학회지
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• 제24권4호
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• pp.556-562
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• 1995

The $\alpha$-amylase inhibitor from naked barley was purified by DEAE-cellulose, Concanavalin-A sepharose and superose 6 column chromatography, and confirmed by capillary electrophoresis. The purified $\alpha$-amylase inhibitor showed a single band of 29KD in molecular weight when estimated by the SDS-PAGE. Its purity was increased by 12-fold as compared to its crude extract, and its specific activity was found to be 336.7units/mg. The major amino acids of the $\alpha$-amylase inhibitor from naked barley was appeared to be glutamic acid, asparitic acid and arginine. The inhibitor from naked barley was glycoproteins and carbohydrate content of inhibitor was 1.0%.