Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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Analysis of dye components using MECC and ion-pairing chromatography

MECC법과 Ion-Pairing 크로마토그래피법을 이용한 염료성분의 분석

  • Jeong, Hyuk (Department of Chemistry, Sookmyung Women's University)
  • 정혁 (숙명여자대학교, 자연과학부, 화학전공)
  • Received : 2006.01.11
  • Accepted : 2006.02.09
  • Published : 2006.02.27
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Abstract

Micellar electrokinetic capillary chromatography(MECC) and HPLC with ion-pairing mechanism were applied for the separation of the well known environmental wastes from dye industry. These compounds include H-acid, J-acid, ${\gamma}$-acid, orthanilic acid, sulfanilic acid and 2-naphthylamine-1,5-disulfonic acid, and are known to be the diazo components of the azo dye. MECC method was also applied to separate few acid dyes including Acid Orange 7, Acid Orange 5 and Acid Blue 92 and direct dye such as Direct Red 80. Informations about the diazo components of any azo dye could be obtained by comparison of electropherogram of the reduction solution of a given dye with those obtained from standard materials such as H-acid, J-acid, ${\gamma}$-acid, orthanilic acid, sulfanilic acid and 2-naphthylamine-1,5-disulfonic acid. It has been concluded that MECC and HPLC with ion-pairing mechanism could be successfully applied for the analysis of unknown dyes and their diazo components.

Ion-Paring을 이용한 고성능 액체 크로마토그래피법과 계면활성제를 완충용액에 섞어서 사용하는 미셀 모세관 전기영동법(micellar electrokinetic capillary chromatography, MECC)을 이용하여 아조염료의 합성성분이면서 동시에 독성을 나타내는 분해물인 H-acid, J-acid, ${\gamma}$-acid, orthanilic acid, sulfanilic acid 그리고 2-naphthylamine-1,5-disulfonic acid 등의 디아조 성분에 대하여 분석을 수행하였다. 같은 방법으로 Acid Orange 7, Acid Orange 5, Acid Blue 92 등의 산성염료와 Direct Red 80 등의 직접염료와 같은 반응성 염료 및 Calcion에 대한 분리를 시도한 결과 모든 염료에 대한 완전한 분리를 얻었으며, 특히 각 염료의 환원용액을 H-acid, J-acid, ${\gamma}$-acid, orthanilic acid, sulfanilic acid 혹은 2-naphthylamine-1,5-disulfonic acid 등의 표준물질과 비교 분석한 결과 사용한 각 염료의 디아조 혹은 커플링 성분을 완벽하게 분석할 수 있음을 알 수 있었고, 따라서 Ion-Pair 크로마토그래피법과 모세관 전기영동법은 미지의 염료에 대한 성분확인 및 디아조 혹은 커플링 성분분석에 응용할 수 있음을 보였다.

Keywords

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