• 한국전자파학회논문지
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• 제14권6호
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• pp.567-574
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• 2003

DS-CDMA 시스템에서 제한된 대역내에 최소의 MAI(Multiple-Access Interference) 값을 갖는 chip waveform design과 그에 대한 성능 평가는 중요하다. 본 논문에서는 참고문헌 ［1］에서 제안된 최적화된 칩 파형 4가지를 근사화하여 해석적 형태로 제시하였고, rectangular, half.sine, raised-cosine 등의 세 가지 기존 파형과 MAI 특성을 비교하여 제안한 칩 파형이 우수함을 확인하였다. 그리고 DPSK 변조방식을 사용한 DS-CDMA 시스템이 Rayleigh와 Nakagami-m 페이딩 채널에서 각 칩 파형의 BER과throughput을 분석하였다. $10^{-3}$ 의 요구 BER에서 수용 가능한 사용자 수를 비교해 보면 제안한 4가지 칩 파형 중 waveform 1의 칩 파형이 기존의 우수한 성능을 는 raised-cosine 파형보다 약 20 % 성능 개선된다. 또한 offered traffic이 30이고 패킷당 비트 수 N$_{d}$가 14인 경우, 최대 throughput을 비교하면 raised-cosine.파형에 비해 최적 waveform 1이 약 18 % 더 우수한 성능을 갖는다.

• 한국통신학회논문지
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• 제36권2B호
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• pp.159-167
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• 2011

LTE-Advanced 시스템은 최대 15bps/Hz의 주파수 효율을 달성하기 위해 상향링크 다중 안테나 전송을 지원해야 한다 본 논문은 LTE-Advanced 상향링크 MIMO 시스템 구조를 제안하고 프리코딩에 의한 링크 적응방식을 고려하여 단말당 오류율을 줄이고 시스템 용량을 향상시키는데 기여할 수 있다 특히, $2{\times}4$ MIMO 시스템에서 최적의 프리코딩 행렬을 선택하여 랭크를 결정하는 방식을 제안하고 MMSE(minimum mean squared error) 수신기에 대한 SINR(signal-to-interference and noise ratio)을 유도한다. 제안 방식의 성능 검증을 위해 실질적인 MIMO 채널 모델에서 BLER(BLock Error Rate) 시뮬레이션을 수행한다. 제안 방식이 full-rank로 고정해서 보내는 경우 보다 더 좋은 성능을 발휘하며 MCS가 낮거나 고속 이동시에 더 큰 이득을 얻을 수 있다.

• Reproductive and Developmental Biology
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• 제35권1호
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• pp.1-8
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• 2011

Techniques to evaluate gene expression profiling, such as sufficiently sensitive cDNA microarrays or real-time quantitative PCR, are efficient methods for monitoring human pluripotent stem cell (hESC/iPSC) cultures. However, most of these high-throughput tests have a limited use due to high cost, extended turn-around time, and the involvement of highly specialized technical expertise. Hence, there is an urgency of rapid, cost-effective, robust, yet sensitive method development for routine screening of hESCs/hiPSCs. A critical requirement in hESC/hiPSC cultures is to maintain a uniform undifferentiated state and to determine their differentiation capacity by showing the expression of gene markers representing all three germ layers, including ectoderm, mesoderm, and endoderm. To quantify the modulation of gene expression in hESCs/hiPSC during their propagation, expansion, and differentiation via embryoid body (EB) formation, we developed a simple, rapid, inexpensive, and definitive multimarker, semiquantitative multiplex RT-PCR platform technology. Among the 9 gene primers tested, 5 were pluripotent markers comprising set 1, and 3 lineage-specific markers were combined as set 2, respectively. We found that these 2 sets were not only effective in determining the relative differentiation in hESCs/hiPSCs, but were easily reproducible. In this study, we used the hES/hiPS cell lines to standardize the technique. This multiplex RT-PCR assay is flexible and, by selecting appropriate reporter genes, can be designed for characterization of different hESC/hiPSC lines during routine maintenance and directed differentiation.

• Asian-Australasian Journal of Animal Sciences
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• 제33권7호
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• pp.1113-1125
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• 2020

Objective: This study was investigated the effects of dietary supplementation of Antrodia cinnamomea fermented product on modulation of antioxidation, anti-inflammation, and lipid metabolism in broilers. Methods: Functional compounds and in vitro antioxidant capacity were detected in wheat bran (WB) solid-state fermented by Antrodia cinnamomea for 16 days (FAC). In animal experiment, 400 d-old broiler chickens were allotted into 5 groups fed control diet, and control diet replaced with 5% WB, 10% WB, 5% FAC, and 10% FAC respectively. Growth performance, intestinal microflora, serum antioxidant enzymes and fatty acid profiles in pectoral superficial muscle were measured. Results: Pretreatment with hot water extracted fermented product significantly reduced chicken peripheral blood mononuclear cells death induced by lipopolysaccharide and 2,2'-Azobis(2-amidinopropane) dihydrochloride. Birds received 5% and 10% FAC had higher weight gain than WB groups. Cecal coliform and lactic acid bacteria were diminished and increased respectively while diet replaced with FAC. For FAC supplemented groups, superoxide dismutase (SOD) activity increased at 35 days only, with catalase elevated at 21 and 35 day. Regarding serum lipid parameters, 10% FAC replacement significantly reduced triglyceride and low-density lipoprotein level in chickens. For fatty acid composition in pectoral superficial muscle of 35-d-old chickens, 5% and 10% FAC inclusion had birds with significantly lower saturated fatty acids as compared with 10% WB group. Birds on the 5% FAC diet had a higher degree of unsaturation, followed by 10% FAC, control, 5% WB, and 10% WB. Conclusion: In conclusion, desirable intestinal microflora in chickens obtaining FAC may be attributed to the functional metabolites detected in final fermented product. Moreover, antioxidant effects observed in FAC were plausibly exerted in terms of improved antioxidant enzymes activities, increased unsaturated degree of fatty acids in chicken muscle and better weight gain in FAC inclusion groups, indicating that FAC possesses promising favorable mechanisms worthy to be developed.

• Nutrition Research and Practice
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• 제8권2호
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• pp.220-226
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• 2014

BACKGROUND/OBJECTIVES: Arsenic, which causes human carcinogenicity, is ubiquitous in the environment. This study was designed to evaluate modulation of arsenic induced cancer by resveratrol, a phytoalexin found in vegetal dietary sources that has antioxidant and chemopreventive properties, in arsenic trioxide ($As_2O_3$)-induced Male Wistar rats. MATERIALS/METHODS: Adult rats received 3 mg/kg $As_2O_3$ (intravenous injection, iv.) on alternate days for 4 days. Resveratrol (8 mg/kg) was administered (iv.) 1 h before $As_2O_3$ treatment. The plasma and homogenization enzymes associated with oxidative stress of rat kidneys were measured, the kidneys were examined histologically and trace element contents were assessed. RESULTS: Rats treated with $As_2O_3$ had significantly higher oxidative stress and kidney arsenic accumulation; however, pretreatment with resveratrol reversed these changes. In addition, prior to treatment with resveratrol resulted in lower blood urea nitrogen, creatinine and insignificant renal tubular epithelial cell necrosis. Furthermore, the presence of resveratrol preserved the selenium content ($0.805{\pm}0.059{\mu}g/g$) of kidneys in rats treated with $As_2O_3$. However, resveratrol had no effect on zinc level in the kidney relative to $As_2O_3$-treated groups. CONCLUSIONS: Our data show that supplementation with resveratrol alleviated nephrotoxicity by improving antioxidant capacity and arsenic efflux. These findings suggest that resveratrol has the potential to protect against kidney damage in populations exposed to arsenic.

• 한국산학기술학회논문지
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• 제12권2호
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• pp.872-877
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• 2011

본 본 논문에서는 블록터보코드를 사용하는 OFDM 시스템에서 오류정정 능력을 향상시키기 위하여 블록터보코드를 디코딩하는데 있어 채널상태정보(CSI)를 이용하는 새로운 알고리듬을 제안한다. 새로운 디코딩 알고리듬은 레일레이 페이딩 환경에서 채널정보를 사용하지 않는 기존의 디코딩 알고리듬과 비교를 하였으며 실험결과 새롭게 제안된 시스템의 경우 1회의 같은 반복 횟수를 가지는 경우에 BER 성능이 신호의 변조방식에 따라 5.0dB~9.0dB 정도의 뛰어난 성능 향상을 보였다. 또한 CSI 값을 적용하고 1회 반복 복호한 시스템이 CSI값을 적용하지 않은 기존의 복호 시스템의 4회 반복 복호한 경우보다도 3.5dB~5dB의 성능 향상을 보임을 알 수 있었다. 따라서 블록터보코드를 반복 복호하는 경우에 CSI 값을 적용한 시스템이 뛰어난 성능 향상과 더불어 반복 복호 시간을 줄임을 알 수 있었다.

• 대한의용생체공학회:의공학회지
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• 제31권1호
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• pp.81-86
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• 2010

Laser irradiation is known to affect various tissues such as skin, bone, nerve, and skeletal muscle. Laser irradiation promotes ATP synthesis, facilitates wound healing, and stimulates cell proliferation and angiogenesis. In skeletal muscle, laser irradiation is related to the proliferation of skeletal muscle satellite cells. Normal skeletal muscle contains remodeling capacity from myogenic cells that are derived from mononuclear satellite cells. Their processes are activated by the expression of genes related with myogenesis such as muscle-specific transcription factors (MyoD and Myf5) and VEGF (vascular endothelial growth factor). In this study, we hypothesized that laser irradiation would enhance and regulate muscle cell proliferation and regeneration through modulation of the gene expressions related with the differentiation of skeletal muscle satellite cells. $C_2C_{12}$ myoblastic cells were exposed to continuous/non-continuous laser irradiation (660nm/808nm) for 10 minutes daily for either 1 day or 5 days. After laser irradiation, cell proliferation and gene expression (MyoD, Myf5, VEGF) were quantified. Continuous 660nm laser irradiation significantly increased cell proliferation and gene expression compared to control, continuous 808nm laser irradiation, and non-continuous 660nm laser irradiation groups. These results indicate that continuous 660nm laser irradiation can be applied to the treatment and regeneration of skeletal muscle tissue.

• Journal of Microbiology and Biotechnology
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• 제15권2호
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• pp.259-264
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• 2005

Transplantation of cultured chondrocytes can regenerate cartilage tissues in cartilage defects in humans. However, this method requires a long culture period to expand chondrocytes to a large number of cells for transplantation. In addition, chondrocytes may dedifferentiate during long-term culture. These problems can potentially be overcome by the use of undifferentiated or partially developed cartilage precursor cells derived from neonatal cartilage, which, unlike chondrocytes from adult cartilage, have the capacity for rapid in vitro cell expansion and may retain their differentiated phenotype during long-term culture. The purpose of this study was to compare the cell growth rate and phenotypic modulation during in vitro culture between adult chondrocytes and neonatal chondrocytes, and to demonstrate the feasibility of regenerating cartilage tissues in vivo by transplantation of neonatal chondrocytes expanded in vitro and seeded onto polymer scaffolds. When cultured in vitro, chondrocytes isolated from neonatal (immediately postpartum, 2 h of age) rats exhibited much higher growth rate than chondrocytes isolated from adult rats. After 5 days of culture, more neonatal chondrocytes were in the differentiated state than adult chondrocytes. Cultured neonatal chondrocytes were seeded onto biodegradable polymer scaffolds and transplanted into athymic mice's subcutaneous sites. Four weeks after implantation, neonatal chondrocyte-seeded scaffolds formed white cartilaginous tissues. Histological analysis of the implants with hematoxylin and eosin showed mature and well-formed cartilage. Alcian blue/ safranin-O staining and Masson's trichrome staining indicated the presence of highly sulfated glycosarninoglycans and collagen, respectively, both of which are the major extracellular matrices of cartilage. Immunohistochemical analysis showed that the collagen was mainly type II, the major collagen type in cartilage. These results showed that neonatal chondrocytes have potential to be a cell source for cartilage tissue engineering.

• IMMUNE NETWORK
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• 제7권4호
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• pp.197-202
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• 2007

Background: Immunomodulators enhancing MHC-restricted antigen presentation would affect many cellular immune reactions mediated by T cells or T cell products. However, modulation of MHC-restricted antigen presentation has received little attention as a target for therapeutic immunoregulation. Here, we report that lectins isolated from mushroom Fomitella fraxinea enhance MHC-restricted exogenous antigen presentation in professional antigen presenting cells (APCs). Methods: Lectins, termed FFrL, were isolated from the carpophores of Fomitella fraxinea, and its effects on the class I and class II MHC-restricted presentation of exogenous ovalbumin (OVA) were examined in mouse dendritic cells (DCs) and mouse peritoneal macrophages. The effects of FFrL on the expression of total MHC molecules and the phagocytic activity were also examined in mouse DCs. Results: DCs cultured in the presence of FFrL overnight exhibited enhanced capacity in presenting exogenous OVA in association with class I and class II MHC molecules. FFrL increased slightly the total expression levels of both class I (H-$2K^b$) and class II (I-$A^b$) MHC molecules and the phagocytic activity of DCs. Antigen presentation-enhancing activity of FFrL was also observed in macrophages isolated from mouse peritoneum. Conclusion: Lectins isolated from the carpophores of Fomitella fraxinea increase MHC-restricted exogenous antigen presentation by enhancing intracellular processing events of phagocytosed antigens.

• 한국통신학회논문지
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• 제39A권2호
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• pp.69-76
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• 2014

MIMO 시스템은 통신 시스템의 채널 용량을 증가시키기 위해서 다양한 분야에서 사용된다. 그러나 이러한 통신 시스템은 다수의 RF 단을 필요로 하기 때문에 안테나 소형화에 문제가 있다. 게다가 다수의 RF 단으로 인하여 아날로그 회로에서의 전력 소모가 증가하고, RF 단의 간섭으로 인하여 통신 효율이 심각하게 저하된다. 이러한 이유 때문에 단일 RF 단을 가지면서도 MIMO 통신이 가능한 BS-MIMO 통신 방식이 제안되었다. BS-MIMO 시스템은 기본적으로 ESPAR 안테나를 사용한다. 기존의 ESPAR 안테나는 5-element 구조를 가지고 있으며, $3{\times}3$ MIMO 전송 까지만 가능하다. 그러므로 MIMO 차원을 확장시키기 위해서는 ESPAR 안테나의 구조 확장이 필수적이다. 본 논문에서는 단일 원 형태의 ESPAR 안테나의 구조 확장을 통해서 BS-MIMO 의 차원을 기존 MIMO 기술처럼 증가시킬 수 있음을 보였다. 설계의 예로써, 13-ESPAR 안테나를 사용하면 $7{\times}7$ BS-MIMO 전송이 가능함을 보였다. 또한 기생 배열안테나의 수가 2개씩 증가할수록 전송 가능한 MIMO 차원이 일씩 증가한다.