• Title/Summary/Keyword: Candidate region

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SNP Discovery in the Leptin Promoter Gene and Association with Meat Quality and Carcass Traits in Korean Cattle

  • Chung, E.R.;Shin, S.C.;Shin, K.H.;Chung, K.Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.12
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    • pp.1689-1695
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    • 2008
  • Leptin, the hormone product of the obese gene, is secreted predominately from white adipose tissue and regulates feed intake, energy metabolism and body composition. It has been considered a candidate gene for performance, carcass and meat quality traits in beef cattle. The objective of this study was to identify SNPs in the promoter region of the leptin gene and to evaluate the possible association of the SNP genotypes with carcass and meat quality traits in Korean cattle. We identified a total of 25 SNPs in the promoter region (1,208-3,049 bp upstream from the transcription start site) of the leptin gene, eleven (g.1508C>G, g.1540G>A, g.1545G>A, g.1551C>T, g.1746T>G, g.1798ins(G), g.1932del(T), g.1933del(T), g.1934del(T), g.1993C>T and g.2033C>T) of which have not been reported previously. Their sequences were deposited in GenBank database with accession number DQ202319. Genotyping of the SNPs located at positions g.2418C>G and g.2423G>A within the promoter region was performed by direct sequencing and PCR-SSCP method to investigate the effects of SNP genotypes on carcass and meat quality traits in Korean cattle. The SNP and SSCP genotypes from the two mutations of the leptin promoter were shown to be associated with the BF trait. The average BF value of animals with heterozygous SNP genotype was significantly greater than that of animals with the homozygous SNP genotypes for the g.2418C>G and g.2423G>A SNPs (p<0.05). Analysis of the combined genotype effect in both SNPs showed that animals with the AC SSCP genotype had higher BF value than animals with BB or AA SSCP genotypes (p<0.05). These results suggest that SNP of the leptin promoter region may be useful markers for selection of economic traits in Korean cattle.

Analysis of SNPs in Bovine CSRP3, APOBEC2 and Caveolin Gene Family (소의 CSRP3, APOBEC2, Caveolin 유전자들의 단일염기다형 분석)

  • Bhuiyan, M.S.A.;Yu, S.L.;Kim, K.S.;Yoon, D.;Park, E.W.;Jeon, J.T.;Lee, J.H.
    • Journal of Animal Science and Technology
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    • v.49 no.6
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    • pp.719-728
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    • 2007
  • The cysteine and glycine rich protein 3 (CSRP3), apolipoprotein B mRNA editing enzyme catalytic polypeptide‐like 2(APOBEC2) and caveolin (CAV) gene family(CAV1, CAV2, CAV3) have been reported to play important roles for carcass and meat quality traits in pig, mouse, human and cattle. As an initial step, we investigated SNPs in these 5 genes among eight different cattle breeds. Eighteen primer pairs were designed from bovine sequence data of NCBI database to amplify the partial gene fragments. Sequencing results revealed 9 SNPs in the coding regions of three caveolin genes, 1 SNP in CSRP3 and 3 SNPs in APOBEC2 gene. All the identified SNPs were confirmed by PCR-RFLP. Also, 9 more intronic SNPs were detected in these genes. However, all identified mutations in the coding region do not change amino acid sequence. Allelic distributions were significantly different for 5 SNPs in CAV2, CAV3, CSRP3 and APOBEC2 genes among the eight different breeds. These results gave some clues about the polymorphisms of these genes among the cattle breeds and will be useful for further searches for identifying association between these SNPs and meat quality traits in cattle.

A User-friendly Remote Speech Input Method in Spontaneous Speech Recognition System

  • Suh, Young-Joo;Park, Jun;Lee, Young-Jik
    • The Journal of the Acoustical Society of Korea
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    • v.17 no.2E
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    • pp.38-46
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    • 1998
  • In this paper, we propose a remote speech input device, a new method of user-friendly speech input in spontaneous speech recognition system. We focus the user friendliness on hands-free and microphone independence in speech recognition applications. Our method adopts two algorithms, the automatic speech detection and the microphone array delay-and-sum beamforming (DSBF)-based speech enhancement. The automatic speech detection algorithm is composed of two stages; the detection of speech and nonspeech using the pitch information for the detected speech portion candidate. The DSBF algorithm adopts the time domain cross-correlation method as its time delay estimation. In the performance evaluation, the speech detection algorithm shows within-200 ms start point accuracy of 93%, 99% under 15dB, 20dB, and 25dB signal-to-noise ratio (SNR) environments, respectively and those for the end point are 72%, 89%, and 93% for the corresponding environments, respectively. The classification of speech and nonspeech for the start point detected region of input signal is performed by the pitch information-base method. The percentages of correct classification for speech and nonspeech input are 99% and 90%, respectively. The eight microphone array-based speech enhancement using the DSBF algorithm shows the maximum SNR gaing of 6dB over a single microphone and the error reductin of more than 15% in the spontaneous speech recognition domain.

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Acoustic Analysis of KSR-III Combustion Chamber with Various 5-Blade Baffles under Non-Reacting Condition (5-블레이드 배플이 설치된 로켓엔진 연소실에서의 상온음향 해석)

  • Kim, Hong-Jip;Kim, Seong-Ku;Sohn, Chae-Hoon
    • 한국연소학회:학술대회논문집
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    • 2003.05a
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    • pp.167-171
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    • 2003
  • Acoustic characteristics of baffled combustion chamber to elucidate suppressing effect of baffle on combustion instability are numerically investigated by linear acoustic analysis. A hub-blade baffle of 5 blades is selected as a candidate one and five variants of baffles with various configuration are designed. Resonant-frequency shift and damping factor are analyzed quantitatively as damping parameters. When the hub is located radially at the pressure node, the decrease of resonant frequency and increase of damping factor in 1R mode are dominant. But sub-1T mode is formed within hub, therefore, there would be a possibility of initiating 1T mode in unbaffled region, which would occur another problem. For smaller hub size, four kinds of axial baffle length is selected. As the axial baffle length increases, resonant frequency shift and increase of damping factor of transverse acoustic modes is obtained. Especially, two close acoustic modes such as 1L and 1T could be overlapped for a certain axial length, resulting in extreme increase of damping factor. The present study based on linear acoustic analysis is expected to be a useful confirming tool to predict acoustic field and design a passive control devices such as baffle and acoustic cavity.

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Microstructure Evolution of 15Cr ODS Steel by a Simple Torsion Test (단순 전단변형에 의한 15Cr 산화물 분산강화 강의 미세조직 변화)

  • Jin, Hyun Ju;Kang, Suk Hoon;Kim, Tae Kyu
    • Journal of Powder Materials
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    • v.21 no.4
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    • pp.271-276
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    • 2014
  • 15Cr-1Mo base oxide dispersion strengthened (ODS) steel which is considered to be as a promising candidate for high- temperature components in nuclear fusion and fission systems because of its excellent high temperature strength, corrosion and radiation resistance was fabricated by using mechanical alloying, hot isostatic pressing and hot rolling. Torsion tests were performed at room temperature, leading to two different shear strain routes in the forward and reverse directions. In this study, microstructure evolution of the ODS steel during simple shearing was investigated. Fine grained microstructure and a cell structure of dislocation with low angle boundaries were characterized with shear strain in the shear deformed region by electron backscattered diffraction (EBSD). Grain refinement with shear strain resulted in an increase in hardness. After the forward-reverse torsion, the hardness value was measured to be higher than that of the forward torsion only with an identical shear strain amount, suggesting that new dislocation cell structures inside the grain were generated, thus resulting in a larger strengthening of the steel.

Face Tracking System Using Updated Skin Color (업데이트된 피부색을 이용한 얼굴 추적 시스템)

  • Ahn, Kyung-Hee;Kim, Jong-Ho
    • Journal of Korea Multimedia Society
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    • v.18 no.5
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    • pp.610-619
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    • 2015
  • *In this paper, we propose a real-time face tracking system using an adaptive face detector and a tracking algorithm. An image is divided into the regions of background and face candidate by a real-time updated skin color identifying system in order to accurately detect facial features. The facial characteristics are extracted using the five types of simple Haar-like features. The extracted features are reinterpreted by Principal Component Analysis (PCA), and the interpreted principal components are processed by Support Vector Machine (SVM) that classifies into facial and non-facial areas. The movement of the face is traced by Kalman filter and Mean shift, which use the static information of the detected faces and the differences between previous and current frames. The proposed system identifies the initial skin color and updates it through a real-time color detecting system. A similar background color can be removed by updating the skin color. Also, the performance increases up to 20% when the background color is reduced in comparison to extracting features from the entire region. The increased detection rate and speed are acquired by the usage of Kalman filter and Mean shift.

An FPGA-based Parallel Hardware Architecture for Real-time Eye Detection

  • Kim, Dong-Kyun;Jung, Jun-Hee;Nguyen, Thuy Tuong;Kim, Dai-Jin;Kim, Mun-Sang;Kwon, Key-Ho;Jeon, Jae-Wook
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.12 no.2
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    • pp.150-161
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    • 2012
  • Eye detection is widely used in applications, such as face recognition, driver behavior analysis, and human-computer interaction. However, it is difficult to achieve real-time performance with software-based eye detection in an embedded environment. In this paper, we propose a parallel hardware architecture for real-time eye detection. We use the AdaBoost algorithm with modified census transform(MCT) to detect eyes on a face image. We parallelize part of the algorithm to speed up processing. Several downscaled pyramid images of the eye candidate region are generated in parallel using the input face image. We can detect the left and the right eye simultaneously using these downscaled images. The sequential data processing bottleneck caused by repetitive operation is removed by employing a pipelined parallel architecture. The proposed architecture is designed using Verilog HDL and implemented on a Virtex-5 FPGA for prototyping and evaluation. The proposed system can detect eyes within 0.15 ms in a VGA image.

Object-based Image Retrieval for Color Query Image Detection (컬러 질의 영상 검출을 위한 객체 기반 영상 검색)

  • Baek, Young-Hyun;Moon, Sung-Ryong
    • Journal of the Institute of Electronics Engineers of Korea CI
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    • v.45 no.3
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    • pp.97-102
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    • 2008
  • In this paper we propose an object-based image retrieval method using spatial color model and feature points registration method for an effective color query detection. The proposed method in other to overcome disadvantages of existing color histogram methods and then this method is use the HMMD model and rough set in order to segment and detect the wanted image parts as a real time without the user's manufacturing in the database image and query image. Here, we select candidate regions in the similarity between the query image and database image. And we use SIFT registration methods in the selected region for object retrieving. The experimental results show that the proposed method is more satisfactory detection radio than conventional method.

STEREO VISION-BASED FORWARD OBSTACLE DETECTION

  • Jung, H.G.;Lee, Y.H.;Kim, B.J.;Yoon, P.J.;Kim, J.H.
    • International Journal of Automotive Technology
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    • v.8 no.4
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    • pp.493-504
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    • 2007
  • This paper proposes a stereo vision-based forward obstacle detection and distance measurement method. In general, stereo vision-based obstacle detection methods in automotive applications can be classified into two categories: IPM (Inverse Perspective Mapping)-based and disparity histogram-based. The existing disparity histogram-based method was developed for stop-and-go applications. The proposed method extends the scope of the disparity histogram-based method to highway applications by 1) replacing the fixed rectangular ROI (Region Of Interest) with the traveling lane-based ROI, and 2) replacing the peak detection with a constant threshold with peak detection using the threshold-line and peakness evaluation. In order to increase the true positive rate while decreasing the false positive rate, multiple candidate peaks were generated and then verified by the edge feature correlation method. By testing the proposed method with images captured on the highway, it was shown that the proposed method was able to overcome problems in previous implementations while being applied successfully to highway collision warning/avoidance conditions, In addition, comparisons with laser radar showed that vision sensors with a wider FOV (Field Of View) provided faster responses to cutting-in vehicles. Finally, we integrated the proposed method into a longitudinal collision avoidance system. Experimental results showed that activated braking by risk assessment using the state of the ego-vehicle and measuring the distance to upcoming obstacles could successfully prevent collisions.

Analysis of Performance Characteristics on Diesel Engine with Aftertreatment and EGR System (후처리 시스템을 장착한 디젤엔진의 EGR 밸브 작동에 따른 성능 분석)

  • Park, Cheol-Woong;Choi, Young;Lim, Gi-Hun
    • Transactions of the Korean Society of Automotive Engineers
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    • v.18 no.5
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    • pp.124-129
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    • 2010
  • The direct injection (DI) diesel engine has become a prime candidate for future transportation needs because of its high thermal efficiency. However, nitrogen oxides (NOx) increase in the local high temperature regions and particulate matter (PM) increases in the diffusion flame region within diesel combustion. Therefore, the demand for developing hybrid system consist of exhaust gas recirculation (EGR) and aftertreatment system as well as diesel particulate filter (DPF) or lean NOx trap (LNT) should be applied. The variation of EGR rate due to the malfunction of EGR valve can affect not only the combustion stability of engine but also the performance of aftertreatment system. In this research, 2.0 liter 4-cylinder turbocharged diesel engine was used to investigate the combustion and emission characteristics for various operating conditions with EGR. While the fuel consumption was increased with increase of EGR rate, NOx emission was improved by maximum 90% at low speed, low load operating condition. To achieve combustion stability and reliability of aftertrearment system with minimum penalty in fuel consumption and emissions, the fault diagnosis of EGR malfunction must be employed.