• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.6
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• pp.2739-2742
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• 2012

6LoWPAN is a standard to enable IPv6 packets to be carried on top of low power wireless networks. It needs to achieve security in 6LoWPAN, which is being defined at the 6LoWPAN working group of the IETF. IPSec is already part of IPv6, which makes it a candidate to be directly employed or adapted for WSNs. Some results showed that the adoption of IPSec is viable, and also point towards the successful design and deployment of security architecture for WSNs. IPSec requires two communicating entities to share a secret key that is typically established dynamically with the IKE. However, there are some limitations to use IKE on wireless networks. In this article, we show requirements for being Efficient Key management Mechanism for IPSec on 6LoWPAN and analyze candidate protocols.

• Parasites, Hosts and Diseases
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• v.56 no.3
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• pp.237-245
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• 2018

Toxoplasma gondii can infect all the vertebrates including human, and leads to serious toxoplasmosis and considerable veterinary problems. T. gondii heat shock protein 60 (HSP60) is associated with the activation of antigen presenting cells by inducing initial immune responses and releasing inflammatory cytokines. It might be a potential DNA vaccine candidate for this parasite. A pVAX-HSP60 DNA vaccine was constructed and immune responses was evaluated in Kunming mice in this study. Our data indicated that the innate and adaptive immune responses was elicited by successive immunizations with pVAX-HSP60 DNA, showing apparent increases of CD3e+CD4+ and CD3e+CD8a+ T cells in spleen tissues of the HSP60 DNA-immunized mice ($24.70{\pm}1.23%$ and $10.90{\pm}0.89%$, P<0.05) and higher levels of specific antibodies in sera. Furthermore, the survival period of the immunized mice ($10.53{\pm}4.78day$) were significantly prolonged during the acute T. gondii infection. Decrease of brain cysts was significant in the experimental group during the chronic infection (P<0.01). Taken together, TgHSP60 DNA can be as a vaccine candidate to prevent the acute and chronic T. gondii infections.

• Journal of Veterinary Science
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• v.24 no.5
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• pp.72.1-72.16
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• 2023

Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) on the surface of Streptococcus dysgalactiae, coded with gapC, is a glycolytic enzyme that was reported to be a moonlighting protein and virulence factor. Objective: This study assessed GAPDH as a potential immunization candidate protein to prevent streptococcus infections. Methods: Mice were vaccinated subcutaneously with recombinant GAPDH and challenged with S. dysgalactiae in vivo. They were then evaluated using histological methods. rGAPDH of mouse bone marrow-derived dendritic cells (BMDCs) was evaluated using immunoblotting, reverse transcription quantitative polymerase chain reaction, and enzyme-linked immunosorbent assay methods. Results: Vaccination with rGAPDH improved the survival rates and decreased the bacterial burdens in the mammary glands compared to the control group. The mechanism by which rGAPDH vaccination protects against S. dysgalactiae was investigated. In vitro experiments showed that rGAPDH boosted the generation of interleukin-10 and tumor necrosis factor-α. Treatment of BMDCs with TAK-242, a toll-like receptor 4 inhibitor, or C29, a toll-like receptor 2 inhibitor, reduced cytokines substantially, suggesting that rGAPDH may be a potential ligand for both TLR2 and TLR4. Subsequent investigations showed that rGAPDH may activate the phosphorylation of MAPKs and nuclear factor-κB. Conclusions: GAPDH is a promising immunization candidate protein for targeting virulence and enhancing immune-mediated protection. Further investigations are warranted to understand the mechanisms underlying the activation of BMDCs by rGAPDH in a TLR2- and TLR4-dependent manner and the regulation of inflammatory cytokines contributing to mastitis pathogenesis.

• Proceedings of the IEEK Conference
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• 2001.09a
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• pp.179-182
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• 2001

In this paper. a key frame extraction algorithm for browsing and searching the summary of a video is proposed. Toward this end, important frames representing a shot are selected according to the correlations among frames. by using the Fourier descriptor which is useful for the shot boundary detection. To quantitatively evaluate the importance of selected frames. a new measure based on correlation coefficients of frames is proposed. If there are several frames with a same importance. another criteria is introduced to break the tie. by computing the partial moment of subframes including each candidate key frame so that the distortion rate is minimized Since a key frame extraction algorithm can be evaluated subjectively. the performance of the proposed algorithm has been verified by a statistical test. Experiments show that more than 20% improvement has been obtained by the proposed algorithm compared to existing methods.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.4
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• pp.494-500
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• 2019

Objective: Feed consumption contributes a large percentage for total production costs in the poultry industry. Detecting genes associated with feeding traits will be of benefit to improve our understanding of the molecular determinants for feed efficiency. The objective of this study was to identify candidate genes associated with feed conversion ratio (FCR) via genomewide association study (GWAS) using sequence data imputed from single nucleotide polymorphism (SNP) panel in a Chinese indigenous chicken population. Methods: A total of 435 Chinese indigenous chickens were phenotyped for FCR and were genotyped using a 600K SNP genotyping array. Twenty-four birds were selected for sequencing, and the 600K SNP panel data were imputed to whole sequence data with the 24 birds as the reference. The GWAS were performed with GEMMA software. Results: After quality control, 8,626,020 SNPs were used for sequence based GWAS, in which ten significant genomic regions were detected to be associated with FCR. Ten candidate genes, ubiquitin specific peptidase 44, leukotriene A4 hydrolase, ETS transcription factor, R-spondin 2, inhibitor of apoptosis protein 3, sosondowah ankyrin repeat domain family member D, calmodulin regulated spectrin associated protein family member 2, zinc finger and BTB domain containing 41, potassium sodium-activated channel subfamily T member 2, and member of RAS oncogene family were annotated. Several of them were within or near the reported FCR quantitative trait loci, and others were newly reported. Conclusion: Results from this study provide valuable prior information on chicken genomic breeding programs, and potentially improve our understanding of the molecular mechanism for feeding traits.

• Animal Bioscience
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• v.36 no.1
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• pp.29-42
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• 2023

Objective: Pigs, an ideal biomedical model for human diseases, suffer from about 50% early embryonic and fetal death, a major cause of fertility loss worldwide. However, identifying the causal variant remains a huge challenge. This study aimed to detect single nucleotide polymorphisms (SNPs) and candidate genes for the number of mummified (NM) piglets using the imputed whole-genome sequence (WGS) and validate the potential candidate genes. Methods: The imputed WGS was introduced from genotyping-by-sequencing (GBS) using a multi-breed reference population. We performed genome-wide association studies (GWAS) for NM piglets at birth from a Landrace pig populatiGWAS peak located on SSC11: 0.10 to 7.11 Mbp (Top SNP, SSC11:1,889,658 bp; p = 9.98E-13) was identified in cyclin dependent kinase on. A total of 300 Landrace pigs were genotyped by GBS. The whole-genome variants were imputed, and 4,252,858 SNPs were obtained. Various molecular experiments were conducted to determine how the genes affected NM in pigs. Results: A strong GWAS peak located on SSC11: 0.10 to 7.11 Mbp (Top SNP, SSC11:1,889,658 bp; p = 9.98E-13) was identified in cyclin dependent kinase 8 (CDK8) gene, which plays a crucial role in embryonic retardation and lethality. Based on the molecular experiments, we found that Y-box binding protein 1 (YBX1) was a crucial transcription factor for CDK8, which mediated the effect of CDK8 in the proliferation of porcine ovarian granulosa cells via transforming growth factor beta/small mother against decapentaplegic signaling pathway, and, as a consequence, affected embryo quality, indicating that this pathway may be contributing to mummified fetal in pigs. Conclusion: A powerful imputation-based association study was performed to identify genes associated with NM in pigs. CDK8 was suggested as a functional gene for the proliferation of porcine ovarian granulosa cells, but further studies are required to determine causative mutations and the effect of loci on NM in pigs.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.8 no.2
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• pp.389-405
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• 2014

Outdated Channel State Information (CSI) was proved to have negative effect on performance in two-way relay networks. The diversity order of widely used opportunistic relay selection (ORS) was degraded to unity in networks with outdated CSI. This paper proposed a multiple relay selection scheme for amplify-and-forward (AF) based two-way relay networks (TWRN) with outdated CSI. In this scheme, two sources exchange information through more than one relays. We firstly select N best relays out of all candidate relays with respect to signal-noise ratio (SNR). Then, the ratios of the SNRs on the rest of the candidate relays to that of the Nth highest SNR are tested against a normalized threshold ${\mu}{\in}[0,1]$, and only those relays passing this test are selected in addition to the N best relays. Expressions of outage probability, average bit error rate (BER) and ergodic channel capacity were obtained in closed-form for the proposed scheme. Numerical results and Simulations verified our theoretical analyses, and showed that the proposed scheme had significant gains comparing with conventional ORS.

• Journal of Microbiology and Biotechnology
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• v.25 no.5
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• pp.681-686
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• 2015

The purpose of this study was to find a cold-adapted and surfactant-stable alginate lyase as a candidate for biotechnological and industrial applications. The gene for a new alginate lyase, AlyL1, from Agarivorans sp. L11 was cloned and expressed in Escherichia coli. The recombinant AlyL1 was most active at 40℃ (1,370 U/mg). It was a cold-adapted alginate lyase, which showed 54.5% and 72.1% of maximum activity at 15℃ and 20℃, respectively. AlyL1 was an alkaliphilic enzyme and most active at pH 8.6. In addition, it showed high stability in the presence of various surfactants at a high concentration (from 0.1% to 1% (w/v)). AlyL1 was an endo-type alginate lyase that degraded both polyM and polyG blocks, yielding disaccharides and trisaccharides as the main products. This is the first report of the cloning and functional expression of a cold-adapted and surfactant-stable alginate lyase. AlyL1 might be an interesting candidate for biotechnological and industrial applications.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.15 no.8
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• pp.2879-2899
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• 2021

The tone reservation (TR) scheme is an attractive method to reduce peak-to-average power ratio (PAPR) in the filter bank multicarrier with offset quadrature amplitude modulation (FBMC-OQAM) systems. However, the high PAPR of FBMC signal will severely degrades system performance. To address this issue, a cyclic shift based TR (CS-TR) scheme with embedding side information (SI) is proposed to reduce the PAPR of FBMC signals. At the transmitter, four candidate signals are first generated based on cyclic shift of the output of inverse discrete Fourier transform (IDFT), and the SI of the selected signal with minimum peak power among the four candidate signals is embedded in sparse symbols with quadrature phase-shift keying constellation. Then, the TR weighted by optimal scaling factor is employed to further reduce PAPR of the selected signal. At the receiver, a reliable SI detector is presented by determining the phase rotation of SI embedding symbols, and the transmitted data blocks can be correctly demodulated according to the detected SI. Simulation results show that the proposed scheme significantly outperforms the existing TR schemes in both PAPR reduction and bit error rate (BER) performances. In addition, the proposed scheme with detected SI can achieve the same BER performance compared to the one with perfect SI.

• Parasites, Hosts and Diseases
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• v.57 no.1
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• pp.61-67
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• 2019

Cystic echinococcosis (CE) in sheep is a hazardous zoonotic parasitic disease that is caused by Echinococcus granulosus (Eg). At present, serological test is an important diagnostic method for Eg infection in domestic animals. Here, a fusion protein Eg mefAg-1 harboring 8 dominant B-cell epitopes of Eg such as antigen B, tetraspanin 1, tetraspanin 6, reticulon and Eg95 was produced in E. coli and evaluated for CE in sheep by indirect ELISA. Eg mefAg-1 showed in ELISA a high sensitivity (93.41%) and specificity (99.31%), with a coincidence rate of 97.02%. Overall, it is suggested that the Eg mefAg-1 could be a potential antigen candidate for CE serodiagnosis in sheep.