• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2000.02a
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• pp.82-87
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• 2000

• Journal of Plant Biology
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• v.32 no.3
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• pp.163-172
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• 1989

Nerium indicum has adaxial colleters on each petiole, bract, bracteole, calyx and corolla. The colleters are finger-shaped sometimes lobed structures with tall heads on short stalks. The petiolar colleters are more abundant than those on the bract, bracteole, calyx and corolla but their structure is similar. Meristematic activity in a small group of protodermal and hypodermal cells at the base of the developing leaf gives rise to the colleter that further differentiates by frequent anticlinal and periclinal divisions. Many of the colleters are vascullarized, sometimes a layer of redially elongated cells is present beneath the epithelial cells. Senescence of a colleter begins when the leaves have either completed their growth or are still expanding. In senescent colleters, the central cell cytoplasm and nuclei are considerably reduced. Thin layer chromatography of the freshly harvested exudate of colleter shows the presence of rhamnose, glucose and arabinose sugars.

• YAKHAK HOEJI
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• v.37 no.6
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• pp.598-604
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• 1993

The calyx extract of Campsis grandiflora displayed inhibitory activity against protein kinase C from the bovine brain. Separation guided by protein kinase C enzyme assay and bleb forming assay led to isolation of a potent protein kinase C inhibitor that was identified as a known phenylpropanoid glycoside, verbascoside. It suppressed completely bleb-formation of K562 cell surface induced by phorbol 12,13-dibutylate at the concentration of 60 $\mu\textrm{g}$/ml and IC$_{50}$ of the protein kinase C occured at 20 $\mu{M}$. This compound was tested for cytotoxic activity against ten human tumor cell lines in vitro. it exhibited moderate cytotoxic activity against skin tumor cell line M14 (IC$_{50}$ 2.2 $\mu\textrm{g}$/ml) and very weak cytotoxicity against other cell lines (IC$_{50}$>10 $\mu\textrm{g}$/ml)

• Animal cells and systems
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• v.7 no.1
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• pp.25-28
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• 2003

Two new species of the genus Stelletta (Astrophorida: Ancorinidae), S. spinulosa n. sp. and S. calyx n. sp., are described from the waters around Gageodo Island (Sohuksando) and Ulleungdo Island, Korea. S. spinulosa n. sp. is similar to S. crassispicula (Sollas, 1886) in the feature of entire animal, but differs from it in the shape of orthotriaene and the form and size of oxyasters. S. calyx n. sp. resembles to S. crater Dendy, 1924 in the shape of sponge, but the new species has four size categories of oxyaster whereas S. carter has no oxyaster.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2000.02a
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• pp.76-81
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• 2000

• The Korean Journal of Physiology and Pharmacology
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• v.8 no.2
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• pp.69-76
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• 2004

A variety of G protein coupled receptors (GPCRs) are expressed in the presynaptic terminals of central and peripheral synapses and play regulatory roles in transmitter release. The patch-clamp whole-cell recording technique, applied to the calyx of Held presynaptic terminal in brainstem slices of rodents, has made it possible to directly examine intracellular mechanisms underlying the GPCR-mediated presynaptic inhibition. At the calyx of Held, bath-application of agonists for GPCRs such as $GABA_B$ receptors, group III metabotropic glutamate receptors (mGluRs), adenosine $A_1$ receptors, or adrenaline ${\alpha}2$ receptors, attenuate evoked transmitter release via inhibiting voltage-activated $Ca^{2+}$ currents without affecting voltage-activated $K^+$ currents or inwardly rectifying $K^+$ currents. Furthermore, inhibition of voltage-activated $Ca^{2+}$ currents fully explains the magnitude of GPCR-mediated presynaptic inhibition, indicating no essential involvement of exocytotic mechanisms in the downstream of $Ca^{2+}$ influx. Direct loadings of G protein ${\beta}{\gamma}$ subunit $(G{\beta}{\gamma})$ into the calyceal terminal mimic and occlude the inhibitory effect of a GPCR agonist on presynaptic $Ca^{2+}$ currents $(Ip_{Ca})$, suggesting that $G{\beta}{\gamma}$ mediates presynaptic inhibition by GPCRs. Among presynaptic GPCRs glutamate and adenosine autoreceptors play regulatory roles in transmitter release during early postnatal period when the release probability (p) is high, but these functions are lost concomitantly with a decrease in p during postnatal development.

• Journal of the East Asian Society of Dietary Life
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• v.3 no.2
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• pp.107-119
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• 1993

The objeative of this study was to investigate the possibility of estimation of internal quality by observing visible state of strawberry. Several tests such as color("L", "A", H,V,C), degree of blackred ness, opening state of calyx, vitamin C content, brix degree, acidity, freshness, overall quality, chlorophyll content, and anthocyanin content were performend during circulation at 20$^{\circ}C$. Acidity(R=-0.96), chloroph-yll content(r=-0.99), vitamin C content(r=-0.98), freshness(r=-0.96), and overall quality(-0.99), degree of blackredness(r=0.98) and opening state of calyx(=0.98), color saturation(r=0.99), degree of blackredness(r=0.98) and opening state of calyx(=0.98) increased during circulation at 20$^{\circ}C$. in correlation between visible indicators andd internal qualities, "L" valu and brix degree was r=-0.74, "a" and brix degree was r=0.93 Hue and anthocyanin content was r=0.74, value and chlorophyll content, brix degree were r=-0.91, r=-0.77, chroma and brix degree was r=0.96. The correlations between color saturation and vitamin C content(r=-0.96), chlorophyll content(r=-0.98), freshness(r=-0.98), overall quality(r=-0.94), loss of total polyphenol(0.94) and acidity(r=-0.94) showed high correlation. The correlatiions between degree of blackrednes and vitamin C content(r=-0.99), chlorophyll content(r=-0.94), anthocyanin content(=-0.74), freshness(r=-0.97) overall quality (r=-0.95), loss of total polyphenol(r=0.93) and acidity(r=-0.91) chlorophyll content(r=-0.99), freshness(r=-0.98), overall quality(r=-0.92), loss of total polyphenol(r=0.99) and acidity(r=0.83) showed high correlation.

• Food Science and Preservation
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• v.17 no.6
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• pp.867-873
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• 2010

The total phenolic contents and the antioxidant activities of methanolic extracts of different parts (fruit, calyx, leaf, stem, and root) of Physalis alkekengi var. francheti were investigated using established in vitro systems including DPPH radical-scavenging, nitrite-scavenging, superoxide anion radical-scavenging activity, measurement of reducing power, and assessment of the metal-chelating effect. The highest extraction yield was from fruit (52.55%), whereas the lowest levels were obtained from root (10.49%) and stem (12.88%).The leaf extract had the highest total phenolic (58.47 mg/g) and total flavonoid (4.83 mg/g) contents, plus the greatest antioxidant activity, as shown by the DPPH radical-scavenging assay, and the highest levels of reducing power at concentrations of 1 and 5 mg/mL. In addition, the calyx also showed good antioxidant activity.These findings indicate that methanolic extracts of leaf and calyx may be useful in the food manufacturing and nutraceutical industries.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.4
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• pp.500-505
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• 2010

The antioxidant activity of methanol extracts from five different parts (flesh, peel, core, seed, calyx) of Jangseong Daebong persimmon (Diospyros kaki cv. Hachiya) were evaluated by determining total phenol content (TPC), DPPH radical scavenging activity (RSA), ABTS RSA, and reducing power (RP). The flesh extract gave the highest yield (92.93%) while the lowest yield was obtained from the seed (5.17%). The seed extract showed the highest total phenolic content ($76.47\pm0.009$ mg GAE/g extract), DPPH RSA ($IC_{50}=52.05\pm1.61\;{\mu}g/mL$), ABTS RSA ($IC_{50}=30.94\pm0.41\;{\mu}g/mL$) and RP ($IC_{50}=87.94\pm0.37\;{\mu}g/mL$). In addition, the calyx extract also showed high antioxidant activity. On the other hand, the core extract gave the lowest TPC and all antioxidant assays. In particular, HT-29 cells showed extensive cell death when treated with $500\;{\mu}g/mL$ of calyx extracts. Thus, these results suggest that methanolic extracts of Jangseong Daebong persimmon seed and calyx may serve as a potential source of natural antioxidant for food and nutraceutical application.

• Journal of Bio-Environment Control
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• v.17 no.2
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• pp.176-180
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• 2008

In days to calyx-flex stage test, flower development affected by light intensity, light duration and the prevailing temperature. As, 'Jacaranda' variety shorter in under 46 days of flower bud formation and 13 days of calyx-flex stage than the other variety with 'Queen Elizabeth' control variety. About cut flower only 'Grand Vicki Brown' (as within 46 days of flower formation, 13 days of calyx stage and 'Jacaranda', both with shorter plants than the other varieties produced cut flower classified as short and medium-stemmed cut flowers. 'Jacaranda', 'Vicki Brown', 'Golden Times', 'Champagne', 'Osiana', and 'Queen Elizabeth (control variety)' flowered earlier from 46.7 to 50.8 days from pruning. Flower development was likewise faster in all the above mentioned cultivars; while 'Tineke' had significantly delayed flower development reaching calyx-flex stage from 0.5 cm bud size after 51.0 days. The top ten high yielding varieties were 'American Orange', with a net income of P3,081,676.00; 'Yellow Island' with P1,572,874.00; 'Frosty Pink' with P1,085,183.00; 'Champagne' with P980,620.00; 'Coral Pink' with P959,669.00; 'Lara Pink' with P918,444.00; 'Lara Orange' with P714,252.00; 'Grand Gala' with P705,787.00; 'Golden Times' with P806,489.00; and 'Lady X' with P778,060.00 net returns.