• Horticultural Science & Technology
• /
• v.17 no.4
• /
• pp.497-499
• /
• 1999

Distribution analysis on natural habitats for the genus Calanthe in Mt. Halla of Cheju province revealed that C. discolor and C. sieboldii were found mostly in the range of 120~400 m and 300~600 m above the sea level, respectively. C. bicolor was found in 300~400 m where both C. discolor and C. sieboldii were overlapped. The present morphological assessments showed that C. discolor had the smallest sizes of leaves and floral parts, C. had the largest, while C. bicolor had the intermediate between the above mentioned two species. Flower colors of C. discolor were brownish red and simple white, and that of C. sieboldii was merely golden yellow, whereas C. bicolor had a diverse mixed colors originated from the other two species. The specific distribution of natural habitats and morphological characteristics of three Calanthe species led the suggestion that C. bicolor can be regarded as new species derived originally from natural hybridization between C. discolor and C. sieboldii.

• Horticultural Science & Technology
• /
• v.17 no.2
• /
• pp.141-143
• /
• 1999

Genetic relationship of Calanthe discolor, C. sieboldii, and C. bicolor for elucidating the classification was investigated. Electrophoretic zymograms for either peroxidase or esterase isozymes indicated that bands of C. bicolor appear in the zone where those of C. discolor and C. sieboldii are located. Genetic relationship among the three Calanthe species using RAPD analysis showed that C. discolor and C. sieboldii are more distant each other than C. bicolor, demonstrating the genetic position of C. bicolor between the other two. It was assumed that C. bicolor is a natural hybrid between C. discolor and C. sieboldii.

• Korean Journal of Plant Taxonomy
• /
• v.42 no.3
• /
• pp.215-221
• /
• 2012

Five Korean Calanthe species, C. discolor, C. bicolor, C. sieboldii, C. reflexa, and C. aristulifera, were studied using amplified fragment length polymorphism (AFLP) to assess their taxonomic and genetic relationships. Sixteen accessions belonging to five native Calanthe spp. and mutants with yellow tepal and white lip (YW mutants) were studied. We identified 50 putative markers using AFLP analysis. The results of AMOVA showed that genetic variance was higher between species than within species. Genetic dissimilarity when compared with the rest of the species was the lowest for individuals of the YW mutants and the highest for individuals of C. reflexa. The mutants clustered outside the major group. Calanthe bicolor clustered with C. discolor, suggesting that its genetic composition is closer to that of C. discolor. Though it is suggested to have originated as a result of natural hybridization between C. sieboldii and C. discolor, introgression is likely to have occurred in the direction of C. discolor based on the data of molecular marker, clustering and genetic dissimilarity. Calanthe reflexa and C. aristulifera were genetically the most diverse of the species studied. In conclusion, the results showed that there is genetic diversity in Korean Calanthe species, that C. bicolor introgressed in the direction of C. discolor and that the YW mutants are genetically closer to C. sieboldii.

• Horticultural Science & Technology
• /
• v.17 no.3
• /
• pp.346-351
• /
• 1999

According to their flower color and the shape of petal, sepal, and lip, Calanthe species native to Cheju Island are named as follows; 'Arang', 'Cheongsu', 'Sara', 'Aeyeol', 'Darigot', 'Saebaengi', 'Andeok', 'Setbyeol', 'Kyorae', 'Jasujeong', 'Baekrok', 'Seokwang', 'Deokcheon', 'Seonheul', 'Saechimi', 'Jokduri', 'Baedal', 'Noksu', 'Noeul', and 'Dansim for 20 cultivars of Calanthe discolor; 'Hwanghee', 'Waheul', 'Illchool', 'Hwangryong', 'Dabo', 'Hwangjinee', 'Daeha', 'Chwikwang', 'Seokyang', 'Eunpa', 'Chwiseon', 'Sinbaram', 'Ara', 'Dano', 'Pakae', and 'Sojeob' for 16 cultivars of Calanthe sieboldii; 'Dongkwang', 'Myeongdeok', 'Wangdolseon', 'Myeongryeok', 'Kwibuin', 'Semi', 'Cheonwangsa', 'Mandeok', 'Seolmundae', 'Kyeongok', 'Darangsi', 'Byeolbang', 'Honghak', 'Dongbaeksan', 'Chejudo', 'Samda', 'Kwandeok', 'Youngjusan', 'Tamra', 'Koyeongdi', 'Yongduam', and 'Jeolbuam' for 22 cultivars of Calanthe bicolor.

• Horticultural Science & Technology
• /
• v.17 no.4
• /
• pp.494-496
• /
• 1999

Natural habitats for Calanthe species were generally established in forests of deciduous broad-leaved trees. The habitat areas had lower summer temperatures, more precipitation, and harrow humidity ranging 75~90% throughout the year than adjacent non-habitat areas. The most influential factor on the habitat establishment was light intensity. The light levels in the habitats are cut down by tree canopies, which also reduce temperature before the defoliation in autumn. The most frequent distribution of the habitat was in the areas with light intensity of 400~1,500 lux under tree canopies and of 30,000~50,000 lux in winter after defoliation. Calanthe was characteristically distributed in the broad-leaved forests where high levels of light are reduced in summer, and cold temperatures in winter are insulated by mulched layers of fallen leaves.

• Korean Journal of Plant Resources
• /
• v.21 no.4
• /
• pp.341-345
• /
• 2008

Two orchid species of Calanthe discolor Lindl. and C. discolor for. sieboldii (Decne.) Ohwi, which have different form flower color and size. They were crossed in mid April by artificial pollination, and the F1 hybrid seeds were collected mid October. Germination of seeds was investigated on pre-treatment of seeds and under the various environmental conditions. Germination was promoted by moisture absorption and ultrasonic treatment of seeds. Dark culture of F1 hybrid seeds enhanced germination and protocorm formation, and development into seedlings compared with light culture. Although, plant growth regulators such as NAA and BA had a slightly promotive effect on seed germination and protocorm growth, regenerated seeding were showed abnormal growth patterns. Regenerated F1 hybrid plantlets were successfully transferred to pot.