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한국산 열당과 종자 형태의 분류학적 적용 (Systematic application of seed morphology In Korean Orobanchaceae)

  • 안범철;홍석표
    • 식물분류학회지
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    • 제33권4호
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    • pp.411-420
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    • 2003
  • 한국산 열당과의 종자형태를 조사하기 위하여 5속 5종 1 변종과 히말라야의 Boschniakia himalaica Hook. f. & Thomson in Hook. f.(총 7 분류군)의 종자를 주사전자현미경을 이용하여 관찰하였다. 한국산 열당과는 종자 크기, 종피의 구조, 과실 당 종자의 수에 따라 두 가지 유형이 구별 되었다. - Type I: 크기가 1mm 미만, 종피 구조는 망상, 과실 당 종자의 개수는 약 1,000개 이상. Type I은 외종피의 내부 표면 무늬에 따라 세 가지 하위구분으로 나뒤어 질 수 있다; (1) Subtype Ia: 망상 -불규칙한 줄무늬형(Ia')과 깊은 망상(Ia")을 포함한다. (2) Subtype Ib: 유공상, (3) Subtype Ic: 평활상. Type II: 크기가 1mm 이상, 종피구조는 불규칙하게 돌출된 모양(creasted), 과실 당 종자의 개수는 약 1,000개 미만(Lathraea japonica Miq.). 한국산 열당과에 대한 종자 형질의 분류학적 적용에 관하여 간략하게 검토하였다.

대구.경북지역 오리 유래 Salmonella속 균의 혈청형, 항균제 내성 및 S. Enteritidis와 S. Typhimurium의 plasmid profiles, phage types 및 PFGE (Serotypes, antimicrobial resistance of Salmonella spp. and plasmid profiles, phage types, PFGE of S. Enteritidis and S. Typhimurium isolated from ducks in Daegu-Gyeongbuk province)

  • 조재근;강민수;김기석
    • 한국동물위생학회지
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    • 제34권3호
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    • pp.217-226
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    • 2011
  • Salmonella spp. is of increasing public health concern as causative pathogens of food poisoning. The aim of this study was to investigate the serotypes and antimicrobial resistance pattern of Salmonella spp. isolated from duck farms in Daegu-Gyeongbuk province. Also, S. Enteritidis and S. Typhimurium isolates were further examined for plasmid analysis, phage typing and pulsed-field gel electrophoresis (PFGE). A total of 34 Salmonella spp. (16.4%) were isolated from duck farms and ten serotypes were identified. The predominant serotypes were S. Typhimurium (23.5%) S. Fyris (17.6%) and S. Haardt (11.8%), S. Agona and S. Enteritidis (respectively 8.8%). Of 34 Salmonella isolates, 15 (44.1%) isolates were resistant to at least one antimicrobial agent and multiple resistance (resistance to more than 4 drugs) was observed in 9 strains (26.5%). The high resistance was found to streptomycin (32.4%), tetracycline (29.4%), ampicillin, kanamycin and nalidixic acid (respectively, 26.5%), all Salmonella isolates were susceptible to cefoxitin, cefotaxime, gentamicin, amikacin and ciprofloxacin. All S. Enteritidis and S. Typhimurium isolates were found to contain only one plasmid (ca. 54 or 55kb, respectively). Among the S. Enteritidis isolates, two phage types were found, PT32a and PT1c, respectively, one isolates did not react with any of the phages used. Whereas, all S. Typhimurium isolates were RDNC (reacts but does not conform). PFGE showed to be a useful typing method better than plasmid analysis and phage typing for discrimination of isolates especially, S. Typhimurium isolates. Our results indicated that the serotypes of Salmonella isolates are widely distributed in duck farms, further epidemiological studies should be carried out.

초등학교 저학년용 영양교육 소책자 개발 및 효과 분석 - 개인별 맞춤형 하루 필요 식품군 단위수 교육을 중심으로 - (Development and Effects' Analysis of Nutrition Education Pamphlet for the Lower Grades Elementary Students - Focused on Individual Daily Needed Food Exchange Units -)

  • 손민정;조영숙;김세나;서혜지;김숙배
    • 대한지역사회영양학회지
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    • 제16권6호
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    • pp.647-660
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    • 2011
  • The purpose of this study was to investigate effects of nutrition education program and pamphlet for the lower grades elementary students focused on individual daily needed food exchange units using Food Exchange System. Program consisted of four lessons (40 min/lesson), "5 major nutrients & function", "6 food group and sources", "daily needed food exchange units for normal body weight", and "smart snack choice and exercise". Pamphlet as activity book was developed for the program. The subjects were 3rd grade elementary students (educated group, 31 vs. non-educated group, 31). Educated group were lessoned as group and/or individual. We examined the differences in nutrition knowledge, dietary attitudes, dietary intakes and satisfaction of the program and pamphlet. In educated group, there were positive improvements on nutrition knowledge score "function and foods of 5 nutrients" and on dietary attitudes "type of breakfast and snacks". In the evaluation of dietary intakes according to KDRI, there were positive improvements on intakes level of riboflavin, vit. C, folate, Ca, P, Fe and Zn in educated group. In satisfaction with the program and pamphlet, contents, font size, visual, figure, difficulty and program curriculum were over 2.90/3.0. It showed that the developed nutrition education program and pamphlet focused on individual daily needed food exchange units using Food Exchange System improved nutrition knowledge, dietary attitudes and nutrients intake level in the lower grades elementary students.

Alginate Bead를 이용한 고분자 약물의 제어방출형 약물수송체 (Alginate Beads as Controlled Release Polymeric Drug Delivery System)

  • 황성주;이계주;조항범;이기명;김종국
    • Journal of Pharmaceutical Investigation
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    • 제23권1호
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    • pp.19-26
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    • 1993
  • The purpose of this paper is to explore the possible applicability of alginate beads as an oral controlled release system of polymeric drugs. Cellulase was used as a model polymeric drug. The release of cellulase from alginate beads was moderately affected by the ratio of cellulase to sodium alginate and strongly affected by $CaCl_2$ concentration. However, the release was not particularly affected by the other factors such as sodium alginate concentration and curing time. The drug was not released from alginate beads at pH 1.2, but was released continuously up to 8 hr at pH 6.8. At pH 6.8, the beads were swollen highly up to 3 hr, thereafter, were eroded into the bulk solution up to 6 hr, completely. Drug release from the beads can be caused due to diffusion and erosion of the matrix. Activity of cellulase was reduced when alginate beads containing cellulase were stored in simulated gastric juice. Further investigation would be necessary to improve the acid resistance of the beads. Since the release of cellulase as a model polymeric drug could be controlled by the regulation of the preparation conditions of alginate beads, the alginate beads may be used for a potential oral controlled release system of such polymeric drugs as polypeptide drugs.

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전주지역 복지관 노인대상 영양교육 및 맞춤형 점심 급식 프로그램 효과 (Effects of Nutrition Education and Personalized Lunch Service Program for Elderly at Senior Welfare Center in Jeonju)

  • 배정숙;김미현;김숙배
    • 대한지역사회영양학회지
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    • 제18권1호
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    • pp.65-76
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    • 2013
  • The purpose of this study was to investigate the effects of a nutrition education and personalized lunch service program in a senior welfare center. A total of 30 elderly (14 males, 16 females) aged 62~89 years participated in this study. Nutrition education lessons (2 hour/lesson/week) were provided for 4 weeks. Also, ten weeks from week 3rd to week 12th, personalized lunch providing 1/3 personal needed energy was served 5 times for a week. After the nutrition intervention program, we compared anthropometric characteristics, blood biochemical characteristics, nutrition knowledge, dietary attitude and dietary intake using 24 hr recall with those before the intervention. The body weight (p < 0.001) and body mass index (BMI) (p < 0.001) were decreased. There were significant increases in score of nutrition knowledge (p < 0.01) and consumption of milk & milk products for snacks. There was a positive effect on fasting blood sugar (FBS) showing significant decreases in portion of impaired fasting glucose and diabetes mellitus (p < 0.05). Also, serum triglyceride (TG) was significantly decreased (p < 0.05). In evaluation of nutrient intake by Dietary Reference Intakes for Koreans (KDRIs), riboflavin (p < 0.01), vitamin C (p < 0.001), calcium (p < 0.05) were positively improved. The index of nutritional quality (INQ) and intakes of vitamin C (p < 0.001), riboflavin (p < 0.05), Ca (p < 0.01) and Fe (p < 0.05) were increased. In conclusion, this nutrition education and lunch service program providing 1/3 personal needed energy can be used to develop and implement a tailored nutritional intervention programs in the setting of a community senior welfare center to improve health and nutritional status of Korean elderly.

폴리카르복시산과 Vitamin C를 이용한 면섬유의 자외선 차단 가공 (UV-cut Finish of Cotton Using Polycarboxylic acid and Vitamin C)

  • 이상욱;최민영;유미듬;장진호
    • 한국염색가공학회:학술대회논문집
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    • 한국염색가공학회 2011년도 제45차 학술발표회
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    • pp.30-30
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    • 2011
  • 산업화 과정에 방출된 할로겐 화합물 및 프레온 가스가 오존층을 파괴하면서 많은 양의 자외선이 지표에 도달하여 생태계에 적지 않은 영향을 끼치고 있다. 자외선은 살균, 소독작용 및 Vitamin D 합성을 위해 생명체에 필수적이지만 과다할 경우 색소침착, 피부노화, 피부암 발생, 백내장의 증가, 면역기능저하 등의 피해를 준다. 자외선은 파장 영역에 따라 UV-A(장파장 자외선), UV-B(중파장 자외선)및 UV-C(단파장 자외선)으로 나뉜다. 이 중 인체에 가장 유해한 것으로 알려진 단파장의 UV-C는 파장이 짧아 침투력이 미약해 오존층과 성층권에서 대부분 흡수되므로 생물학적으로 큰 의미는 없다. 그러나 자외선 중 UV-B와 UV-A 영역의 자외선은 인체에 영향을 가장 많이 미치므로 이들 영역의 자외선을 차단하는 것이 필요하다. Vitamin C는 아스코르브산(ascorbic acid)이라고도 불리며 미백효과, 주름 개선, 자외선 차단 및 항산화 작용을 하지만 섬유가공 시 Vitamin C는 쉽게 산화되며 세탁 내구성이 떨어지기 때문에 가교제를 필요로 한다. Vitamin C를 이용하여 자외선 차단성 섬유제품을 개발하기 위해, 포름알데히드를 유리하지 않는 폴리카르복시산 가교제 중 하나인 Citric Acid(CA)를 사용하여 고온에서 면직물에 처리했을 때 에스테르 형 가교를 형성하여 내세탁성을 가질 수 있었다. 자외선 차단 효과를 알아보기 위하여 KS K 0850 섬유제품의 자외선 차단율 및 차단지수 시험방법으로 평가하였다.

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강원도 영서지역 출토 분청사기의 조성 및 미세구조 분석: 원주(原州) 법천사지(法泉寺址)를 중심으로 (Compositional and microstructural analyses of grayish-blue-powdered celadon in Yeongseo Region in Gangwon-do:by Wonju Beopcheon-temple site)

  • 이병훈;소명기
    • 분석과학
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    • 제26권3호
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    • pp.211-221
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    • 2013
  • 본 연구는 원주 법천사지에서 출토된 분청사기의 화학조성과 미세조직, 결정상을 X-선형광분석기와 주사전자현미경-에너지분산형엑스선분석기, X-선회절분석기를 이용하여 알아보았다. 태토의 화학성분 분석 결과, 낮은 실리카($RO_2$) 함량과 높은 융제(RO+$R_2O$) 함량임을 알 수 있다. 유약의 경우 융제 성분 중 CaO 함량이 높은 전형적인 석회계열(lime type)과 석회-알칼리 계열(lime-alkali type) 유약을 사용하였으며 태토의 결정상으로는 quarts, mullite, microcline, albite가 확인되었다. 특히 원주 법천사지에서 출토된 분청사기 태토의 화학적 조성은 제겔식을 이용하여 이미 연구된 다른 유적에서 출토된 분청사기와 비교하였으며 화학조성은 서로 상이한 결과를 보였다.

전자현미경을 이용한 건물내 비고형 표면자재의 석면 오염 및 기중 석면농도 특성조사 (Characterization of Asbestos Content in Friable Sprayed-on Surface Material and Airborne Asbestos Concentrations in Buildings by TEM)

  • 유성환;김현욱
    • 한국산업보건학회지
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    • 제6권2호
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    • pp.165-175
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    • 1996
  • Fourteen(14) large commercial buildings located in Seoul with friable sprayed-on surface insulation material on ceiling were investigated for fiber types in bulk material and for airborne fiber concentrations in buildings by transmission electron microscopy (TEM) in order to compare the results with those by polarized light microscopy (PLM) and phase contrast microscopy (PCM). The results were as follows: 1. Chrysotile asbestos was found in one bulk sample out of total 14 bulk samples collected. Glass fiber and mineral wool were the two major constituents of the bulk samples. 2. The Na-Mg-Si-Ca-Fe-Al ratios of the EDX spectra which were normalized with the Si peak were 0-1.0-10-8.3-4.0-4.0 in mineral wool and 0-5-10-21-0-0 in chrysotile asbestos, respectively. 3. Airborne fiber concentrations were log-normalcy distributed and the geometric mean (geometric standard deviation) fiber concentrations by TEM in the underground parking lots and inside buildings were 0.0048 f/cc(1.93) and 0.0040 f/cc(2.27), respectively with no statistical difference. In the outdoor ambient air, statistically significantly lower concentration of 0.0018 f/cc(2.04) was measured. 4. The TEM/PCM ratios of airborne fiber concentrations ranged 0.5 - 2.0 for 80 % of airborne samples analyzed, end the regression equation between TEM and PCM was PCM=-0.2724+1.1355(TEM) with the coefficient of determination $R^2=0.52$. The results of this study confirmed that the sprayed-on surface insulation material found in some commercial buildings may possibly be contaminated with asbestos fiber. Since statistically significant relationship of fiber concentrations measured by PCM and TEM inside buildings and ambient air was found, previous results by PCM in ambient air could be used to estimate the ambient fiber concentrations in knowing the ratio of TEM/PCM.

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DNA 사슬 종결형 항암제인 플루다라빈에 의해 유도된 세포독성에 대한 아로니아-홍삼 에탄올 혼합 추출물의 효과 (Effects of Aronia melanocarpa and Korean Red Ginseng Ethanol Extracts Combination on Cytotoxicity induced by Fludarabine, a DNA Chain Terminating Anti-Cancer Drug)

  • 김민섭;정유헌;오홍근;박종군
    • 한국식품영양학회지
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    • 제30권4호
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    • pp.673-680
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    • 2017
  • Fludarabine, a chain terminating anti-cancer drug, is a purine analogue that causes DNA strand breaks in normal cells. In this study, we determined if A. melanocarpa and Korean red ginseng extract mixture reduce cytotoxicity of fludarabine. Treatment of HaCaT cells with $10{\mu}M$ of fludarabine for 24 hours decreased cell viability and increased DNA strand breaks. Treatment of A. melanocarpa and Korean red ginseng extract mixture for 24 hours increased cell viability as compared with single extract treatment. The protective effect of these extracts on cell activity increased in a concentration-dependent manner. DNA strand breaks induced by fludarabine decreased as concentration of extract mixture increased. p-H2AX level, a marker of DNA strand breakage, decreased depending on the concentration of extract mixture. The effect of mixed extract of A. melanocarpa and Korean red ginseng on DNA damage is due to the anti-oxidative effect of A. melanocarpa and signal transmission through glucocorticoid receptor upon binding of saponin of Korean red ginseng.

산화구리 나노입자가 분산된 CNT fiber 유연 전극 기반의 글루코스 검출용 비효소적 전기화학센서 (Electrochemical Sensor for Non-Enzymatic Glucose Detection Based on Flexible CNT Fiber Electrode Dispersed with CuO Nanoparticles)

  • 송민정
    • Korean Chemical Engineering Research
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    • 제61권1호
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    • pp.52-57
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    • 2023
  • 본 연구는 고성능 유연 전극 소재 개발을 위한 기초 연구로, 유연 전극 소재의 성능을 향상시키기 위해 금속 산화물 CuO nanoparticles (CuO NPs)를 도입하여 탄소나노튜브 섬유(carbon nanotube fiber; CNT fiber) 표면 위에 전기화학적 증착시켜 CNT fiber/CuO NPs 전극을 합성하고, 이를 전기화학적 비효소 글루코스 센서에 적용하였다. 이 전극의 표면 및 elemental composition 분석은 주사전자 현미경(SEM)과 에너지분산형 분광분석법(EDS)을 이용하였으며, 전극의 전기화학적 특성 및 글루코스에 대한 센싱 성능은 순환전압 전류법(CV)과 전기화학 임피던스법(EIS), 시간대전류법(CA)을 통해 조사되었다. CNT fiber/CuO NPs 전극은 CNT fiber의 우수한 특성과 함께 CuO NPs 도입에 따른 약 2.6배의 유효 전극면적(active surface area) 증가 효과와 11배 정도의 향상된 전자전달(electron transfer) 특성 및 우수한 전기적 촉매 활성(electrocatalytic activity) 덕분에 CNT fiber 유연 기반 전극의 글루코스 검출에 대한 성능이 개선되었다. 따라서, 본 연구를 기반으로 다양한 나노구조체를 활용한 고성능 유연 전극 소재 개발이 기대된다.