Browse > Article
http://dx.doi.org/10.7853/kjvs.2011.34.3.217

Serotypes, antimicrobial resistance of Salmonella spp. and plasmid profiles, phage types, PFGE of S. Enteritidis and S. Typhimurium isolated from ducks in Daegu-Gyeongbuk province  

Cho, Jae-Keun (Metropolitan Health & Environmental Research Institute)
Kang, Min-Su (National Veterinary Research and Quarantine Service)
Kim, Ki-Seuk (College of Veterinary Medicine, Kyungpook National University)
Publication Information
Korean Journal of Veterinary Service / v.34, no.3, 2011 , pp. 217-226 More about this Journal
Abstract
Salmonella spp. is of increasing public health concern as causative pathogens of food poisoning. The aim of this study was to investigate the serotypes and antimicrobial resistance pattern of Salmonella spp. isolated from duck farms in Daegu-Gyeongbuk province. Also, S. Enteritidis and S. Typhimurium isolates were further examined for plasmid analysis, phage typing and pulsed-field gel electrophoresis (PFGE). A total of 34 Salmonella spp. (16.4%) were isolated from duck farms and ten serotypes were identified. The predominant serotypes were S. Typhimurium (23.5%) S. Fyris (17.6%) and S. Haardt (11.8%), S. Agona and S. Enteritidis (respectively 8.8%). Of 34 Salmonella isolates, 15 (44.1%) isolates were resistant to at least one antimicrobial agent and multiple resistance (resistance to more than 4 drugs) was observed in 9 strains (26.5%). The high resistance was found to streptomycin (32.4%), tetracycline (29.4%), ampicillin, kanamycin and nalidixic acid (respectively, 26.5%), all Salmonella isolates were susceptible to cefoxitin, cefotaxime, gentamicin, amikacin and ciprofloxacin. All S. Enteritidis and S. Typhimurium isolates were found to contain only one plasmid (ca. 54 or 55kb, respectively). Among the S. Enteritidis isolates, two phage types were found, PT32a and PT1c, respectively, one isolates did not react with any of the phages used. Whereas, all S. Typhimurium isolates were RDNC (reacts but does not conform). PFGE showed to be a useful typing method better than plasmid analysis and phage typing for discrimination of isolates especially, S. Typhimurium isolates. Our results indicated that the serotypes of Salmonella isolates are widely distributed in duck farms, further epidemiological studies should be carried out.
Keywords
Salmonella; Antimicrobial resistance; Plasmid; Phage type; PFGE;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Yang SJ, Park KY, Kim SH, No KM, Besser TE, Yoo HS, Kim SH, Lee BK, Park YH. 2002. Antimicrobial resistance in Salmonella enterica serovars Enteritidis and Typhimurium isolated from animals in Korea: comparison of phenotypic and genotypic resistance characterization. Vet Microbiol 86: 295-301.   DOI
2 김상윤, 이희무, 김신, 홍현표, 권헌일. 2001. 경북지역 가축에서 분리한 Salmonella typhimurium과 S. enteritidis의 phage typing 및 pulsed-field gel electrophoresis. 한국가축위생학회지 24: 243-253.   과학기술학회마을
3 양하영, 이성모, 박은정, 김정희, 이정구. 2009. 인천지역 닭 도축장에서 분리된 Salmonella spp.의 항생제 내성 및 PFGE 패턴분석. 한국가축위생학회지 32: 325-334.   과학기술학회마을
4 우용구, 박미선, 우승룡, 김봉환, 김재학. 2000b. 한국의 동물과 사람에서 분리한 Salmonella enteritidis의 phage types. 대한수의학회지 40: 515-524.
5 우용구, 이희수, 이영주, 강민수, 김봉환, 김재학. 2000a. 우리나라의 가금과 환경에서 분리한 Salmonella species의 특성. 대한수의학회지 40: 505-514.
6 이우원, 정병렬, 이강록, 이동수, 김용환. 2009. 소와 돼지유래 다제내성 Salmonella속 균의 분자유전학적 특성. 한국가축위생학회지 32: 61-75.   과학기술학회마을
7 정석찬, 송시욱, 김성일, 정명은, 김계희, 이지연, 임숙경. 2007. 국내 도축장에서 분리한 세균의 항생제 감수성 조사 (2. 도축장의 식육으로부터 분리한 Salmonella 균의 항생제 감수성). 한국수의공중보건학회지 31: 51-56.   과학기술학회마을
8 질병관리본부. 2010. 2009년도 우리나라 급성설사질환 유발 원인 세균의 분리현황 및 특성. Public health Weekely Report 3: 545-552.
9 Anderson ES, Ward LR, Saxe MJ, de Sa JD. 1977. Bacteriophage-typing designations of Salmonella typhimurium. J Hyg(Lond) 78: 297-300.
10 Barrow PA. 1993. Salmonella control-past, present and future. Avian Pathol 22: 651-669.   DOI
11 Brunner F, Margadant A, Peduzzi R, Piffaretti JC. 1983. The plasmid pattern as an epidemiologic tool for Salmonella typhimurium epidemics: comparison with the lysotype. J Infect Dis 148: 7-11.   DOI
12 Carlson SA, Bolton LF, Briggs CE, Hurd HS, Sharma VK, Fedorka-Cray PJ, Jones BD. 1999. Detection of multiresistant Salmonella typhimurium DT104 using multiplex and fluorogenic PCR. Mol Cell Probes 13: 213-222.   DOI
13 CDC, PulseNet. 2004. Standardized laboratory protocol for molecular subtyping of Escherchia coli O157:H7, non-typhoidal Salmonella serotypes, and Shigella sonnei by Pulsed-Field Gel Electrophoresis (PFGE). http://www.cdc.gov/pulsenet/protocols/ecoli_salmonella_shigella_protocols.pdf.
14 Cheong HJ, Lee YJ, Hwang IS, Kee SY, Cheong HW, Song JY, Kim JM, Park YH, Jung JH, Kim WJ. 2007. Characteristics of non-typhoidal Salmonella isolates from human and broiler-chickens in southwestern Seoul, Korea. J Korean Med Sci 22: 773-778.   DOI
15 Clinical and Laboratory Standards Institute. 2009. Performance standards for antimicrobial disk susceptibility test, Approved standard, 10th ed. CLSI document M02-A10. Wayne, Pennsylvania.
16 Ewing W. 1986. Edward and Ewing's Identification of Enterobacteriaceae. 4th ed. pp. 27-45. Elsevier Science Publishing Co. New York.
17 Gast RK. 2008. Salmonella infections. pp. 619-674. In: Saif YM, Glisson JR, Fadly AM, McDougald LR, Nolan L(ed.). Disease of Poultry. 12th ed. Iowa State University Press. Ames, Iowa.
18 Gatto AJ, Peters TM, Green J, Fisher IS, Gill ON, O'brien SJ, Maguire C, Berghold C, Lederer I, Gerner-Smidt P, Torpdahl M, Siitonen A, Lukinmaa S, Tschape H, Prager R, Luzzi I, Dionisi AM, VAN DER Zwaluw WK, Heck M, Coia J, Brown D, Usera M, Echeita A, Threlfall EJ. 2006. Distribution of molecular subtypes within Salmonella enterica serotype Enteritidis phage type 4 and S. Typhimurium definitive phage type 104 in nine European countries. 2000-2004: results of an international multi-centre study. Epidemiol Infect 134: 729-736.   DOI
19 Kado CI, Liu ST. 1981. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol 145: 1365-1373.
20 Guerra B, Schrors P, Mendoza MC. 2000. Application of PFGE performed with XbaI to an epidemiological and phylogenetic study of Salmonella serotype typhimurium. Relations between genetic types and phage types. New Microbiol 23: 11-20.
21 Kang ZW, Jung JH, Kim SH, Lee BK, Lee DY, Kim YJ, Lee JY, Won HK, Kim EH, Hahn TW. 2009. Genotypic and phenotypic diversity of Salmonella enteritidis isolated from chickens and humans in Korea. J Vet Med Sci 71: 1433-1438.   DOI
22 Kessel AS, Gillespie IA, O'Brien SJ, Adak GK, Humphrey TJ, Ward LR. 2001. General outbreaks of infectious intestinal disease linked with poultry, England and Wales, 1992-1999. Commun Dis Public Health 4: 171-177.
23 Kim SH, Kim S, Chun SG, Park MS, Park JH, Lee BK. 2008. Phage types and pulsed-field gel electrophoresis patterns of Salmonella enterica serovar Enteritidis isolated from humans and chickens. J Microbiol 46: 209-213.   과학기술학회마을   DOI
24 Kimura AC, Reddy V, Marcus R, Cieslak PR, Mohle-Boetani JC, Kassenborg HD, Segler SD, Hardnett FP, Barrett T, Swerdlow DL, Emerging Infections Program FoodNet Working Group. 2004. Chicken consumption is a newly identified risk factor for sporadic Salmonella enterica serotype Enteritidis infections in the United States: a case-control study in FoodNet sites. Clin Infect Dis 38 Suppl 3: S244-S252.   DOI
25 Ogasawara N, Tran TP, Ly TL, Nguyen TT, Iwata T, Okatani AT, Watanabe M, Taniguchi T, Hirota Y, Hayashidani H. 2008. Antimicrobial susceptibilities of Salmonella from domestic animals, food and human in the Mekong Delta, Vietnam. J Vet Med Sci 70: 1159-1164.   DOI
26 Mare L, Van Der Walt ML, Dicks LM. 2000. Phage types of Salmonella enterica serovar Enteritidis isolated in South Africa from 1991-1995. Onderstepoort J Vet Res 67: 129-133.
27 Ridley AM, Threlfall EJ, Rowe B. 1998. Genotypic characterization of Salmonella enteritidis phage types by plasmid analysis, ribotyping, and pulsed-field gel electrophoresis. J Clin Microbiol 36: 2314-2321.
28 Murase T, Okitsu T, Suzuki R, Morozumi H, Matsushima A, Nakamura A, Yamai S. 1995. Evaluation of DNA fingerprinting by PFGE as an epidemiologic tool for Salmonella infections. Microbiol Immunol 39: 673-676.   DOI
29 National Committee for Clinical Laboratory Standards. 2003. Performance standards for antimicrobial disk susceptibility tests, 8th infomational supplement: approved standard M2-A8. NCCLS, Wayne, PA, USA.
30 Poppe C, Johnson RP, Forsberg CM, Irwin RJ. 1992. Salmonella enteritidis and other Salmonella in laying hens and eggs from flocks with Salmonella in their environment. Can J Vet Res 56: 226-232.
31 Rodrigue DC, Tauxe RV, Rowe B. 1990. International increase in Salmonella enteritidis: a new pandemic? Epidemiol Infect 105: 21-27.   DOI
32 Saitanu K, Jerngklinchan J, Koowatananukul C. 1994. Incidence of salmonellae in duck eggs in Thailand. Southeast Asian J Trop Med Public Health 25: 328-331.
33 Tassios PT, Gazouli M, Tzelepi E, Milch H, Kozlova N, Sidorenko S, Legakis NJ, Tzouvelekis LS. 1999. Spread of a Salmonella typhimurium clone resistant to expanded- spectrum cephalosporins in three European countries. J Clin Microbiol 37: 3774-3777.
34 Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, Swaminathan B. 1995. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33: 2233-2239.
35 Terajima J, Nakamura A, Watanabe H. 1998. Epidemiological analysis of Salmonella enterica Enteritidis isolates in Japan by phage-typing and pulsed-field gel electrophoresis. Epidemiol Infect 120: 223-229.   DOI
36 Tsai HJ, Hsiang PH. 2005. The prevalence and antimicrobial susceptibilities of Salmonella and Campylobacter in ducks in Taiwan. J Vet Med Sci 67: 7-12.   DOI
37 Threlfall EJ, Rowe B, Ward LR. 1993. A comparison of multiple drug resistance in salmonellas from humans and food animals in England and Wales, 1981 and 1990. Epidemiol Infect 111: 189-197.   DOI
38 Timoney JF, Gillespie JH, Scott FW, Barlough JE. 1988. Hagan and Bruner's Microbiology and Infectious Diseases of Domestic Animals. 8th ed. pp. 74-86. Cornell University Press, Ithaca and London.
39 Tran TP, Ly TL, Nguyen TT, Akiba M, Ogasawara N, Shinoda D, Okatani TA, Hayashidani H. 2004. Prevalence of Salmonella spp. in pigs, chickens and ducks in the Mekong Delta, Vietnam. J Vet Med Sci 66: 1011-1014.   DOI
40 Tsen HY, Lin JS. 2001. Analysis of Salmonella enteritidis strains isolated from food-poisoning cases in Taiwan by pulsed field gel electrophoresis, plasmid profile and phage typing. J Appl Microbiol 91: 72-79.   DOI
41 van Duijkeren E, Wannet WJ, Houwers DJ, van Pelt W. 2002. Serotype and phage type distribution of Salmonella strains isolated from humans, cattle, pigs, and chickens in the Netherlands from 1984 to 2001. J Clin Microbiol 40: 3980-3985.   DOI
42 Ward LR, de Sa JD, Rowe B. 1987. A phage-typing scheme for Salmonella enteritidis. Epidemiol Infect 99: 291-294.   DOI
43 WHO. 2003. Joint FAO/OIE/WHO 2nd Workshop on Non-human antimicrobial usage and antimicrobial resistance, scientific assessment. Geneva, 1-5 December.
44 Wray C, Wray A. 2000. Salmonella in domestic animals. pp. 157-167. CABI Publishing. New York, USA.