• Title/Summary/Keyword: Ca transport

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Nutrient Dynamics through Water Transport in Natural Deciduous Hardwood Forests in Chunchon, Kangwon Province (강원도 춘천지역 낙엽활엽수림에서의 수분이동에 따른 양분동태)

  • 진현오;손요환;이명종;박인협;김동엽
    • The Korean Journal of Ecology
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    • v.26 no.1
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    • pp.13-18
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    • 2003
  • We examined water flux, concentrations and contents in nutrients in precipitation, throughfall, stemflow and soil solution in natural deciduous hardwood forest(Quercus variabilis and Q. mongolica) in Chunchon, Kangwon Province. The volume of throughfall was 2∼3% higher in Q. variabilis than in Q. monglica while volume of stemflow, Ao, A and B soil solution was 10∼15% higher in Q. variabilis compared to Q. monglica. Concentrations of K/sup +/ increased in throughfall H while concentrations of Ca/sup 2+/, Mg/sup 2+/ and NO₃/sup -/ increased in Ao soil solution. The former might be related to the canopy leaching and the latter related to leaching and nitrification in Ao horizon. Nutrient concentrations in throughfall, Ao, A and B soil solution decreased with increasing amount of water and especially the decreases in concentrations of K/sup +/, Mg/sup 2+/ and Cl/sup -/ were significant. Nutrient concentrations of Ca/sup 2+/ in Ao soil solution was 1.5 times higher in Q. variabilis than in Q. mongolica. However, there were no significant nutrient concentration differences in throughfall, stemflow and A and B soil solution between the two forest types. Stemflow was less than 10% of total water volume (throughfall + stemflow) to the forest floor, and contribution of stemflow to nutrient cycling seemed to be low in the study forest.

A Study on Factors Influencing the Severity of Autonomous Vehicle Accidents: Combining Accident Data and Transportation Infrastructure Information (자율주행차 사고심각도의 영향요인 분석에 관한 연구: 사고데이터와 교통인프라 정보를 결합하여)

  • Changhun Kim;Junghwa Kim
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.22 no.5
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    • pp.200-215
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    • 2023
  • With the rapid advance of autonomous driving technology, the related vehicle market is experiencing explosive growth, and it is anticipated that the era of fully autonomous vehicles will arrive in the near future. However, along with the development of autonomous driving technology, questions regarding its safety and reliability continue to be raised. Concerns among technology adopters are increasing due to media reports of accidents involving autonomous vehicles. To promote the improvement of the safety of autonomous vehicles, it is essential to analyze previous accident cases and identify their causes. Therefore, in this study, we aimed to analyze the factors influencing the severity of autonomous vehicle accidents using previous accident cases and related data. The data used for this research primarily comprised autonomous vehicle accident reports collected and distributed by the California Department of Motor Vehicles (CA DMV). Spatial information on accident locations and additional traffic data were also collected and utilized. Given that the primary data used in this study were accident reports, a Poisson regression analysis was conducted to model the expected number of accidents. The research results indicated that the severity of autonomous vehicle accidents increases in areas with low lighting, the presence of bicycle or bus-exclusive lanes, and a history of pedestrian and bicycle accidents. These findings are expected to serve as foundational data for the development of algorithms to enhance the safety of autonomous vehicles and promote the installation of related transportation infrastructure.

Regulation of Mg2+ efflux by cAMP in perfused rat heart and isolated ventricular myocytes (흰쥐의 심장과 심근세포에서 cyclic AMP에 의한 Mg2+ 유리조절)

  • Kang, Hyung-sub;Kim, Jin-shang;Kang, Chang-won;Lee, Ho-il
    • Korean Journal of Veterinary Research
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    • v.39 no.1
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    • pp.62-69
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    • 1999
  • Although it has been reported that hormones or chemicals, which increase in intracellular cAMP, produced $Mg^{2+}$ release from the heart, it is not well characterized whether a specific $Mg^{2+}$ exchanger is involved in cAMP-induced $Mg^{2+}$ efflux in the mammalian hearts. In this work, we studied the relationship between the increase in intracellular cAMP and ion transport system on $Mg^{2+}$ regulation in the perfused rat heart and isolated myocytes. The $Mg^{2+}$ content in the perfusate and supernatant were measured by atomic absorption spectrophotometer. The addition of membrane permeable cAMP analogue to the perfused hearts and myocytes induced a $Mg^{2+}$ efflux in the dose dependent manners. $Mg^{2+}$ efflux was stimulated by cAMP modulators (forskolin, IBMX and Ro20-1724) in the perfused hearts and myocytes. cAMP-induced $Mg^{2+}$ efflux was inhibited by $H_7$, benzamil or imipramine in the perfused hearts and myocytes, but not by EIPA. We confirmed that a significant $Mg^{2+}$ efflux was induced by an increase in intracellular cAMP in the hearts and myocytes. The cAMP-induced increase of $Mg^{2+}$ efflux in the hearts may be involved in ion transport system ($Na^+-Ca^{2+}$ and $Na^+-Mg^{2+}$ exchanger).

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Clay mineral distribution and provenance in surface sediments of Central Yellow Sea Mud

  • Koo, HyoJin;Lee, YunJi;Kim, SoonOh;Cho, HyenGoo
    • Geosciences Journal
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    • v.22 no.6
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    • pp.989-1000
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    • 2018
  • The provenance of the Central Yellow Sea Mud (CYSM) in the Yellow Sea has been attracted a great deal of attention over the last three decades, but a consensus is not yet reached. In this study, 101 surface sediment samples collected from the CYSM were investigated to determine provenance and transport mechanisms in the area using the clay minerals and major element components. The Huanghe sediments are characterized by higher smectite, but the Changjiang sediments are more abundant illite contents. Western Korean rivers contain more kaolinite and chlorite than do Chinese rivers. The Chinese rivers have higher $Fe_2O_3$, MgO, and CaO than the Korean rivers at the same $Al_2O_3$ concentration. Therefore, the clay minerals and major element concentrations can be useful indicator for the source. Based on our results, we suggest that the surface sediments in CYSM were composed mainly of Changjiang sediments, mixed a partly with sediments from the Huanghe and the western Korean rivers. Although the northwestern part of CYSM is proximate to the Huanghe, the contents of smectite and CaO were extremely low. It could be evidence that the Huanghe materials do not enter directly into the CYSM due to the Shandong Peninsula Front. Considering the oceanic circulation in the Yellow Sea, the Changjiang sediments could be transported eastward with the Changjiang Diluted Water and then mixed in CYSM via the Yellow Sea Warm Current (YSWC). Huanghe sediments could be provided by coastal currents (Shandong Coastal Current and Yellow Sea Coastal Current) and the YSWC. In addition, sediments from western Korean rivers might be supplied into the CYSM deposit via the Korean Coastal Current, Transversal Current, and YSWC.

Characteristics of Chloride Ion Behavior in an Cement Matrix Using Calcium Nitrite Inhibitor (아질산칼슘 방청제를 사용한 시멘트 경화체 내의 염소이온 거동 특성)

  • Min-Cheol Shin;Ki-Yong Ann
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.12 no.2
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    • pp.206-213
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    • 2024
  • The present study concerns the inhibition of Calcium Nitrite Inhibitor(Ca(NO2)2) in mortar contaminated by chloride ions. Thus, the corrosion resistance and chloride transport were measured for the mortar containing calcium nitrite inhibitor. As a result, an increase in the dosage of calcium nitrite inhibitor resulted in an increase in the chloride threshold concentration for reinforcement corrosion, while the rate of chloride transport was accelerated. However, the calcium nitrite inhibitor could not guarantee the time to corrosion, due to the increased mobility of chlorides. To ensure the passivity of steel, the dosage of calcium nitrite inhibitor must exceed a certain dosage, ranging from 2.0~3.0 % by cement weight.

Traffic Accidents Scenarios Based on Autonomous Vehicle Functional Safety Systems (자율주행차량 기능안전 시스템 기반 사고 시나리오 도출)

  • Heesoo Kim;Yongsik You;Hyorim Han;Min-je Cho;Tai-jin Song
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.22 no.6
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    • pp.264-283
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    • 2023
  • Unlike conventional vehicle traffic accidents, autonomous vehicles traffic accidents can be caused by various factors, including technical problems, the environment, and driver interaction. With the future advances in autonomous driving technology, new issues are expected to emerge in addition to the existing accident causes, and various scenario-based approaches are needed to respond to them. This study developed autonomous vehicle traffic accident scenarios by collecting autonomous driving accident reports, CA DMV collision reports, autonomous driving mode disengagement reports, and autonomous driving actual accident videos. The scenarios were derived based on the functional safety system failure modes of ISO 26262 and attempted to reflect the various issues of autonomous driving functions. The autonomous vehicle scenarios derived through this study are expected to play an essential role in preventing and preparing for various autonomous vehicle traffic accidents in the future and improving the safety of autonomous driving technology.

Relaxant Actions of α2-Adrenoceptor Agonists in Rat Aorta (α2-Adrenoceptor Agonists의 흰쥐 대동맥 이완 작용)

  • Cho, In-gook;Lee, Sang-woo;Kang, Hyung-sub;Seo, Heyng-seok;Kim, Jin-shang
    • Korean Journal of Veterinary Research
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    • v.43 no.3
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    • pp.361-371
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    • 2003
  • The vasorelaxant actions and blood pressure lowering of the ${\alpha}_2$-adrenoceptor agonists (${\alpha}_2$-AAs) clonidine and xylazine were investigated in rat isolated aortic rings and anesthesized rats. Both clonidine and xylazine produced a concentration-dependent inhibition of the sustained contraction induced by norepinephrine (NE), but not by KCl. NE-induced contractions were attenuated partly by nifedipine or verapamil, voltage dependent $Ca^{2+}$ channel blockers. These $Ca^{2+}$ channel blockers-resistant contractions were abolished by clonidine or xylazine. Inhibitory effects of a ${\alpha}_2$-AAs on contractions could be reversed by ryanodine, an intracellular $Ca^{2+}$, transport blocker, and tetrabutylammonium (TBA), a $Ca^{2+}$ activated $K^+$ channel blocker, but not by nifedipine, glibenclamide or removal of extracellular $Ca^{2+}$ and endothelium. Moreover, ${\alpha}_2$-AAs produced relaxation in NE-precontracted isolated intact aortic rings in a concentration-dependent manner, but not in KCl-precontracted rings. The relaxant effects of ${\alpha}_2$-AAs were inhibited by ryanodine and TBA, but not by nifedipine, glibenclamide, N (G)-nitro-L-arginine (L-NNA), N(omega)-nitro-L-arginine methyl ester (L-NAME), aminoguanidine (AG), 2-nitro-4-carboxyphenyl N,N-diphenylcarhurnte (NCDC), lithium sulfate, staurosporine or removal of extracellular $Ca^{2+}$ and endothelium. In vivo, infusion of xylazine elicited significant decrease in anerial blood pressure. This xylazinelowered blood pressure was completely inhibited by the intravenous injection of TBA, but not by the intravenous injection of glibenclamide, L-NNA, L-NAME, AG, nifedipine, lithium sulfate or saponin.. These findings showed that the receptor-mediated and ${\alpha}_2$-adrenoceptor A-stimulated endothelium-independent vasorelaxant effect may be explained by decreasing intracellular $Ca^{2+}$ release and activation of $Ca^{2+}$-activated $K^+$ channels, which may contribute to the hypotensive effects of ${\alpha}_2$-AAs in rats.

[$Ca^{2+}-activated\;K^+$ Currents of Pancreatic Duct Cells in Guinea-pig

  • Lee, Han-Wook;Li, Jing Chao;Koo, Na-Youn;Piao, Zheng Gen;Hwang, Sung-Min;Han, Jae-Woong;Choi, Han-Saem;Lee, Jong-Heun;Kim, Joong-Soo;Park, Kyung-Pyo
    • The Korean Journal of Physiology and Pharmacology
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    • v.8 no.6
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    • pp.335-338
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    • 2004
  • There are numerous studies on transepithelial transports in duct cells including $Cl^-$ and/or $HCO_3^-$. However, studies on transepithelial $K^+$ transport of normal duct cells in exocrine glands are scarce. In the present study, we examined the characteristics of $K^+$ currents in single duct cells isolated from guinea pig pancreas, using a whole-cell patch clamp technique. Both $Cl^-$ and $K^+$ conductance were found with KCI rich pipette solutions. When the bath solution was changed to low $Cl^-$, reversal potentials shifted to the negative side, $-75{\pm}4\;mV$, suggesting that this current is dominantly selective to $K^+$. We then characterized this outward rectifying $K^+$ current and examined its $Ca^{2+}$ dependency. The $K^+$ currents were activated by intracellular $Ca^{2+}$. 100 nM or 500 nM $Ca^{2+}$ in pipette significantly (P<0.05) increased outward currents (currents were normalized, $76.8{\pm}7.9\;pA$, n=4 or $107.9{\pm}35.5\;pA$, n=6) at +100 mV membrane potential, compared to those with 0 nM $Ca^{2+}$ in pipette $(27.8{\pm}3.7\;pA,\;n=6)$. We next examined whether this $K^+$ current, recorded with 100 nM $Ca^{2+}$ in pipette, was inhibited by various inhibitors, including $Ba^{2+}$, TEA and iberiotoxin. The currents were inhibited by $40.4{\pm}%$ (n=3), $87.0{\pm}%$ (n=5) and $82.5{\pm}%$ (n=9) by 1 mM $Ba^{2+}$, 5 mM TEA and 100 nM iberiotoxin, respectively. Particularly, an almost complete inhibition of the current by 100 nM iberiotoxin further confirmed that this current was activated by intracellular $Ca^{2+}$. The $K^+$ current may play a role in secretory process, slnce recycling of $K^+$ is critical for the initiation and sustaining of $CI^-$ or $HCO_3^-$ secretion in these cells.

Gene Expression Profiling by Ginsenoside Rb1 in Keratinocyte HaCaT Cells (피부각질세포 HaCaT에서 진세노사이드 Rb1에 의한 유전자 발현 양상)

  • Lee, Dong Woo;Kim, Jung Min;Bang, In Seok
    • Journal of Life Science
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    • v.29 no.5
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    • pp.514-523
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    • 2019
  • We investigated the gene expression patterns and the mechanisms of action of the apoptotic response by microarray analysis of human keratinocyte HaCaT cells treated with ginsenoside Rb1, a saponin of Panax ginseng C. A. Meyer. Genes related to apoptosis, the G2/M transition of the mitotic cell cycle, cell division, mitotic nuclear division, and intracellular protein transport were 2-fold up-regulated in HaCaT cells treated with the ginsenoside Rb1, whereas genes related to DNA repair, regeneration fission, and extracellular matrix organization were 2-fold down-regulated. Apoptosis signaling may be mediated by FAS and PLA2G4A, and pathway analysis indicated that STAT3 might be an upstream regulator of these genes. The activity of FAS and PLA2G4A was verified by qPCR, which showed that FAS was increased about 2-fold in HaCaT cells treated with $10{\mu}g/ml$ of ginsenoside Rb1 for 24 hr, PLA2G4A was increased about twice after 6 hours, and gene expression was increased more than 2-fold after 24 hr. Knockdown of STAT3 with siRNA decreased FAS expression and increased PLA2G4A expression but only FAS was passed from the upstream regulator STAT3. These results indicate that STAT3, which is an upstream regulator, induces apoptosis via FAS during treatment with ginsenoside Rb1.

Transport and Distribution of Calcium Salt in Tofu Manufacturing Process -Part I. Conductometric Measurement of Calcium Salt in Tofu and Drained Solution- (두부제조공정(製造工程)중 Calcium 염(鹽)의 행동(行動)과 분포(分布) -제1보(第一報). 전기전도도법(電氣傳導度法)에 의한 두부 및 순물의 Calcium 농도 측정방법(測定方法)-)

  • Yim, Sang-Bin;Lee, Choon-Ki;Chun, Jae-Kum
    • Applied Biological Chemistry
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    • v.28 no.1
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    • pp.8-12
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    • 1985
  • For measuring calcium content during the manufacturing process of Tofu, conductometric electrodes were made with the copper plate, and the results were compared with chemical analysis methods. Three types of plate material (I, II, III) for electrode were tested to apply for measuring $CaCl_2$ content in solution, drained solution from Tofu and in agar-agar gel ana Tofu. Empirical linear correlation equations between conductivity (Y, mho) and calcium content (C, Mole $\bar{C}$, mg% wet basis) were obtained for the quick estimation of calcium content during Tofu processings. Equations with plate II type electrode were Y=0.6364C+0.0775 for drained solution with r=0.99, and $Y=7.1503{\times}10^{-5}\bar{C}-2.9895{\times}10^{-3}$ for Tofu with r=0.91,respectively.

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