• Title/Summary/Keyword: CW

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A Characteristics of Biological Resources of Portunus trituberculatus (Miers, 1876) around the Chilsan Inland Younggwang, Korea (영광 칠산도 주변해역 꽃게, Portunus trituberculatus (Miers, 1876)의 생물자원학적 특성)

  • An, Yun-Keun;Choi, Sung-Min;Choi, Sang-Duk;Yoon, Ho-Seop
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.18 no.2
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    • pp.115-122
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    • 2012
  • Frequency distribution of carapace width, relative growth, sex ratio and fecundity of blue crab Portunus trituberculatus in Younggwang adjacent water were investigated. A total number of individuals collected for this study were 658(Males : 305 and females : 353). Water temperature and salinity of the study areas varied from 5.9 to $27.3^{\circ}C$ and 28.1 to 31.6 psu, respectively. The carapace length-width and carapace width-weight relationship was calculated and the equations obtained are; For males; CL = 1.3652CW + 22.495($R^2$=0.741), $TW=0.0068CW^{2.4001}$($R^2$=0.749), For females; CL = 1.3073 CW + 25.684($R^2$=0.791), $TW=0.0061CW^{2.4231}$($R^2$=0.801). The sex ratios(Males : females) for blue crab were 0.86 : 1. The value of correlations coefficient between total weight and fecundity of the blue crabs was higher that of between carapace width and fecundity.

Detection of Heartbeat and Respiration Using a Modified Signal Model in the CW Bio-Radar (CW 바이오 레이더에서 수정 송수신 모델을 이용한 심장 박동 및 호흡 검출)

  • Seo, Myung-Hwan;Lee, Byung-Seub
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.19 no.11
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    • pp.1204-1212
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    • 2008
  • The paper proposes a new signal model which is revised from the commonly used signal model. Recently, many research institutions had a research about CW bio-radar for detecting he heartbeat and respiration. However, when the bio-radar detects the heartbeat using the previous signal model, the bio-radar has a disadvantage of weakness about he residual phase and AWGN. Also, the model is inappropriate in ergonomics because this signal model supposes hat the heart and lung are located at a same place. In this paper, the modified signal model, which is appropriate n ergonomics, is proposed. This paper analyzes and compares with the performance for detecting the heartbeat and respiration using the previous model and revised model in AWGN and multi-path environment.

Transformation of Leuconostoc mesenteroides SY1, a Strain Isolated from Kimchi

  • JEONG SEON-JU;PARK JAE-YONG;KIM JONG HWAN;KIM GYEONG MIN;CHUN JIYEON;LEE JONG-HOON;CHUNG DAE-KYUN;KIM JEONG HWAN
    • Journal of Microbiology and Biotechnology
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    • v.16 no.1
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    • pp.149-152
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    • 2006
  • Leuconostoc mesenteroides SY1, a strain isolated from cabbage Kimchi, was transformed with pCW4, a shuttle vector based on a cryptic plasmid from Lactobacillus paraplantarum C7. $\alpha-Amylase$ gene, amyL, from Bacillus licheniformis was cloned into pCW4, resulting in $pCW4T{\alpha},\;and\;pCW4T{\alpha}$ was introduced into SY1 by electroporation. Transformation efficiency was $10^2cells/{\mu}g$ plasmid DNA. L. mesenteroides cells harboring $pCW4T{\alpha}$ did not show amylase activity, although amyL transcript was synthesized as determined by slot blot experiment. $pCW4T{\alpha}$ was stably maintained in SY1 in the presence of erythromycin (Em, $5\;{\mu}g/ml$) but rapidly lost when Em was omitted. Less than $1\%$ of the cells maintained $pCW4T{\alpha}$ after 5 days at $30^{\circ}C$.

Extraction of protein from defatted sesame meal using the enzyme from Bacillus sp. CW-1121 (Bacillus sp. CW-1121이 생성하는 단백 분해 효소를 이용한 참깨박 단백질의 용출)

  • Choi, C.;Chun, S.S.;Cho, Y.J.
    • Applied Biological Chemistry
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    • v.36 no.2
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    • pp.121-126
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    • 1993
  • To extract insoluble proteins of sesame meal residue by using microorganism, the sesame meal residue was treated with crude enzyme solution from Bacillus sp. CW-1121. It was found that the solubility reached to maximum at pH 7.5, $45^{\circ}C$. Under optimum condition, the nitrogen solubility with the enzyme solution from Bacillus sp. CW-1121 reached to 60% in 2 hours. Nitrogen solubility of protein from sesame meal showed minimum value at pH 4.5 and significantly increased above pH 6.0. When the protein from sesame meal extracted with Bacillus sp. CW-1121 was subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis, water soluble protein was showed 4 bands and salt soluble protein was showed 2 bands. The amino acid composition of water soluble protein, salt soluble protein and free amino acid indicated relatively high contents of serine (17.24 mg/g), glutamic acid (10.77 mg/g) and glutamic acid (6.55 mg/g). Specially, the contents of essential amino acids were high.

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Microscopic Identification-keys for Cynanchi Wilfordii Radix and Cynanchi Auriculati Radix (白首烏와 耳葉牛皮消의 내부형태 감별기준)

  • Doh, Eui-Jeong;Kim, Jung-Hoon;Choi, Goya;Lee, Seung-Ho;Song, Ho-Joon;Ju, Young-Sung;Lee, Guem-San
    • The Korea Journal of Herbology
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    • v.30 no.4
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    • pp.65-69
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    • 2015
  • Objectives : The root ofCyanchum wifordii(CW) had been used as herbal medicine, 'Baeksuo', in Korea. However, the root ofCynanchum auriculatum(CA) had also been mis-used as 'Baeksuo' in herbal markets, due to its morphological similarity with CW. This study aimed to compare discriminative features and establish the identification-keys between two herbal medicines using microscopic methods.Methods : Microscopic preparations including fixation, rapid dehydration, paraffin infiltration, paraffin embedding, sectioning, mounting, and staining, were performed and the microscopic features between CW and CA samples were compared.Results : The starch grain of parenchymal cells, the amounts of calcium oxalate crystals, cell sizes, and the shape of phloem were not different between CW and CA samples. However, the clusters of stone cells which were conglomerated at outer cortex were apparently observed in CA sample (4~8 layers of stone cells), but not in CW sample (2~4 layers of stone cells, or not). Xylem of CW consisted of several vessels and fiber bundles which were formed as linear shapes and discontinuous radial lines, while that of CA, in which radial lines was not observed, were shaped as a wedge and scattered near cambium with comparatively few fiber bundles enclosed vessels. Especially, in the xylem of CA, fiber bundles located towards cambium were relatively more developed than those located outward cambium.Conclusions : The identification-keys established in this study would be helpful to discriminate microscopic features between CW and CA.

Effects of Catechins and Wheat Bran on the Beef Color in the Late Fattening Period of Hanwoo Steers

  • Kim, Do-Hyung;Oh, Young-Kyoon;Jang, Sun-Sik;Kwon, Eung-Gi;Seo, Yong-Joo;Ok, Ji-Un;Park, Keun-Kyu;Lee, Sung-Sill;Kim, Kyoung-Hoon
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.6
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    • pp.832-838
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    • 2012
  • This study was conducted to evaluate the effects of dietary supplementation of catechins and wheat bran on growth performance, carcass characteristics and meat color using forty-eight Hanwoo steers. Each steer was randomly assigned to one of four treatments. Treatments were as follows: control, catechins, wheat bran, and catechins+wheat bran (CW). At the end of the feeding trial, blood samples were collected for analysis of hemoglobin and iron concentration, and then steers were slaughtered at a commercial abattoir. Catechins, wheat bran, and CW had no effect on growth performance and carcass characteristics. Percentage of myoglobin in M. longissimus dorsi was not affected by treatments. Percentage of oxymyoglobin was increased (p<0.05) by CW and maintained at the highest level (p<0.05) for CW during meat display time up to 24 h. Percentage of metmyoglobin was the lowest (p<0.05) for CW between 5 and 24 h during display time. Although blood iron concentration was not affected by treatments, total concentration of muscle iron was the lowest (p<0.05) for CW compared with that of other treatments. Therefore, the results of the present study suggest that CW may provide good protection against oxidation of myoglobin, and might be an effective dietary supplement for improving meat color in the late fattening period of Hanwoo steers.

Effects of Cynanchum Wilfordii Extract on In vitro Ruminal Fermentation Characteristics and Methane Production (백하수오 추출물이 In vitro 반추위 발효성상 및 메탄가스 생성에 미치는 영향)

  • Yang, Seung-Hak;Lim, Joung-Soo;Kim, Byul;Hwang, Ok-Hwa;Cho, Sung-Back;Choi, Dong-Yoon;Choi, Seok-Geun;Hwang, Seong-Gu
    • Journal of Animal Environmental Science
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    • v.19 no.2
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    • pp.155-162
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    • 2013
  • The objective of this study is to investigate the effects of Cynanchum wilfordii (CW) on cell viability, anti-oxidant activity, volatile fatty acid (VFA) production and methane gas production. Collected rumen fluid incubated with CW powder (1% w/v) for 12 and 24 hours were analyzed for pH, VFAs and methane. Alamar blue assay showed no significant difference on the viability of 3T3-L1 and C2C12 cells treated with CW for 24 hours. TBARS data showed a dose dependent increase on the antioxidant activity of CW. VFAs increased in the CW-treated groups compared to the control group. In addition, propionate increased more than other VFAs by the treatment with CW. There was a significant decrease in methane gas production in batch culture treated with CW in 12hrs. In conclusion, it was suggested that Cynanchum wilfordii could manipulate rumen fermentation considered by increasing VFA production and inhibition of methanogenesis.

Detection Algorithm for Information on Approach or Deviation of Objects Using CW Doppler Radar and FFT (CW 도플러 레이더와 FFT를 이용한 물체의 접근 이탈 정보 판단 알고리즘)

  • Shin, Hyun-Jun;Han, Byung-Hun;Oh, Chang-Heon
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2015.10a
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    • pp.999-1001
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    • 2015
  • CW Doppler radar is capable of giving the relative velocity of an object using the Doppler effect. When detecting more than an object, frequency domain analysis is needed using CW Doppler radar and FFT. Even though the number of objects and velocities can be obtained within the frequency domain, there is a disadvantage that it is difficult to assess information on approach or deviation of an object. When detecting more than an object using FFT, this study suggests an algorithm for efficiently assessing information about approach or deviation of objects within the frequency domain. The proposed algorithm divides sections into real and imaginary numbers in the frequency domain, and then determines deviation if the total sum of the amplitudes of each frequency is on the left side and approach if the total sum of the amplitudes is on the right side.

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Anti-obesity effect of Cynanchi Wilfordii Radix on High fat diet-induced obese mice (고지방식이(高脂肪食餌)로 유도된 동물모델에서 백수오(白首烏)의 항비만(抗肥滿) 효과)

  • Fang-lan, Ouyang;Seo, Bu Il
    • The Korea Journal of Herbology
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    • v.34 no.2
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    • pp.49-58
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    • 2019
  • Objectives : Obesity is a public health concern associated with chronic diseases including hyperlipidemia, diabetes, fatty liver, atherosclerosis and cancer. As several anti-obesity drugs have been limited owing to their side effects, the development of new anti-obesity drugs through herbal medicines has been increasing. Cynanchum Wilfordii Radix (CW) traditionally is consumed for various health benefits including immune enhancing, anti-inflammation and anti-tumor activities. The aim of the present study is to evaluate the effects of CW on High fat diet (HFD)-induced obese mice. Methods : The mice were randomly divided into four groups (n=7). The mice were respectively fed a normal diet (ND), a high-fat diet (HFD), HFD plus CW (50 mg/kg/day), HFD plus CW (100 mg/kg/day). All groups were assayed for body weights, food efficiency ratio, blood biochemistry parameters, and organic tissue weights. Results : HFD-fed mice showed an increase in the body weight and serum biochemistry parameters levels (total cholesterol and triglycerides) as well as organic tissue weights. However, the administration of CW to obese mice induced a reduction in their body weight, food efficiency ratio, blood biochemistry parameters and weight of liver and fat compared with the HFD fed mice. Additionally, we observed that CW inhibited the lipid accumulation in liver and serum lipid parameter induced by HFD. Conclusions : Taken together, the findings of this study suggest that CW may be a potential agent for use in the treatment of obesity and obesity-related metabolic diseases.

Physiological Activities and Inhibitory Effect of Extracts of Cynanchi wilfordii Radix and Perilla sikokiana against Cell Differentiation in 3T3-L1 Adipocytes (백하수오와 자소엽 추출물의 생리활성 및 3T3-L1 전지방세포에 대한 분화 억제 효과)

  • Kim, Seon Jeong;Kang, Seung Mi;Ko, Keon Hee;Nam, Sanghae
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.5
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    • pp.642-650
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    • 2016
  • Cynanchi wilfordii Radix (CW) and Perilla sikokiana (PS) were extracted under different conditions to study their antioxidant, anti-diabetic, and anti-obesity activities. Their potentials as functional food ingredients were investigated. The highest total phenol contents were $15.74{\pm}0.69mg/g$ for CW100 [100% fermented ethanol (FE) extract from CW] and $39.37{\pm}3.46mg/g$ for PS50 (50% FE extract from PS). When extracts were processed at 1 mg/mL, DPPH radical scavenging activities were $79.79{\pm}0.79%$ and $82.69{\pm}1.07%$, respectively, at CW100 and PS50. ABTS radical scavenging activities were $80.20{\pm}2.86%$ and $75.00{\pm}1.78%$, respectively, at CW100 and PS50. However, ferric reducing antioxidant power activities at 1 mg/mL were higher than 80% for PS under all extraction conditions. The highest ${\alpha}$-amylase inhibitory activities were $51.56{\pm}0.56{\sim}59.2{\pm}1.13%$ at CW50 and $46.70{\pm}0.32{\sim}66.17{\pm}0.55%$ at PS0. Cell differentiation inhibitory effects in 3T3-L1 adipocytes were $29.49{\pm}2.98%$ at CW100 and $23.31{\pm}0.61%$ at PS50. The inhibitory effect of the CW100-PS50 mixture was $43.03{\pm}1.63%$, which was significantly higher than those of individual extracts.