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http://dx.doi.org/10.6116/kjh.2015.30.4.65.

Microscopic Identification-keys for Cynanchi Wilfordii Radix and Cynanchi Auriculati Radix  

Doh, Eui-Jeong (Center for Metabolic Function Regulation, Wonkwang Univ.)
Kim, Jung-Hoon (School of Korean Medicine, Pusan National Univ.)
Choi, Goya (Korea Institute of Oriental Medicine)
Lee, Seung-Ho (Korea Institute of Oriental Medicine)
Song, Ho-Joon (College of Korean Medicine, Wonkwang Univ.)
Ju, Young-Sung (College of Korean Medicine, Woosuk Univ.)
Lee, Guem-San (College of Korean Medicine, Wonkwang Univ.)
Publication Information
The Korea Journal of Herbology / v.30, no.4, 2015 , pp. 65-69 More about this Journal
Abstract
Objectives : The root ofCyanchum wifordii(CW) had been used as herbal medicine, 'Baeksuo', in Korea. However, the root ofCynanchum auriculatum(CA) had also been mis-used as 'Baeksuo' in herbal markets, due to its morphological similarity with CW. This study aimed to compare discriminative features and establish the identification-keys between two herbal medicines using microscopic methods.Methods : Microscopic preparations including fixation, rapid dehydration, paraffin infiltration, paraffin embedding, sectioning, mounting, and staining, were performed and the microscopic features between CW and CA samples were compared.Results : The starch grain of parenchymal cells, the amounts of calcium oxalate crystals, cell sizes, and the shape of phloem were not different between CW and CA samples. However, the clusters of stone cells which were conglomerated at outer cortex were apparently observed in CA sample (4~8 layers of stone cells), but not in CW sample (2~4 layers of stone cells, or not). Xylem of CW consisted of several vessels and fiber bundles which were formed as linear shapes and discontinuous radial lines, while that of CA, in which radial lines was not observed, were shaped as a wedge and scattered near cambium with comparatively few fiber bundles enclosed vessels. Especially, in the xylem of CA, fiber bundles located towards cambium were relatively more developed than those located outward cambium.Conclusions : The identification-keys established in this study would be helpful to discriminate microscopic features between CW and CA.
Keywords
Cynanchum wilfordii; Cynanchum auriculatum; identification; authentication; microscopy;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
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