• Journal of Microbiology
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• 제39권4호
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• pp.300-304
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• 2001

Epstein-Barr Virus(EBV)-transformed lymphoblastoid B cell lines, BLCL which expresse antigens, are potential antigen-presenting cells(APCs) for the induction of CTL in vitro. However transfection of BLCLs with subsequent selection by antibiotics is notoriously difficult because plating efficiencies of BLCLsare reported to be 1% or less. To generated stable transfectants of BLCLs we produced high titers of retroviruess encoding pp 65 antigen of human cytomegalovirus of foreign antigens and trans-duced them of BLCLs. The pp 65 gene was cloned into the retroviral vector pLXSN. The recombinant retroviral vector was transfected to ecotropic packaging cell line, CP&E86, and this polyclonal recom-binant retrovirus was transduced to PA317 that is amphotropic pakaging cell line. The titers of colned PA317 amphotropic retroviruses ranged from 5 to $\times$10$^{6}$ colony forming units (CFU)per ml (CFU/ml) We performed three rounds of consecutive transductions to BLCLs in order to improve the clon-ing effieiencies. The expression of recombinant HCMV-pp65 antigen was more than 20% after the final transduction. THe third-transduced BLCLs were easily selected in optimal concentration of G418. BLCLs expressing foreign antigens could be used as target cells for CTL assay and/or as APCs for induction of in vitro CTL responses specific for viral and tumor antigens.

• IMMUNE NETWORK
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• 제11권2호
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• pp.100-106
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• 2011

Background: Disparities of Minor H antigens can induce graft rejection after MHC-matched transplantation. H60 has been characterized as a dominant antigen expressed on hematopoietic cells and considered to be an ideal model antigen for study on graft-versus-leukemia effect. Methods: Splenocytes from C57BL/6 mice immunized with H60 congenic splenocytes were used for establishment of H60-specific CTL clones. Then the clones were characterized for proliferation capacity and cytotoxicity after stimulation with H60. Clone #14, #15, and #23 were tested for the TCR binding avidity to H60-peptide/$H-2K^b$ and analyzed for TCR sequences. Results: H60-specific CTL clones showed different levels of proliferation capacity and cytotoxic activity to H60-stimulation. Clones #14, #15, and #23 showed high proliferation activity, high cytotoxicity, and low activities on both aspects, respectively, and have TCRs with different binding avidities to H60-peptide/$H-2K^b$ with $t_{1/2}$ values of 4.87, 6.92, and 13.03 minutes, respectively. The TCR usages were $V{\alpha}12D-3-01+J{\alpha}11-01$ and $V{\beta}12-1-01+D{\beta}1-01+J2-7-01$ for clone #14, $V{\alpha}13D-1-02+J{\alpha}34-02$ and $V{\beta}13-1-02+D{\beta}2-01+J{\beta}2-7-01$ for clone #15, and $V{\alpha}16D+J{\alpha}45-01$ and $V{\beta}12-1-01+D{\beta}1-01+J{\beta}2-5-01$ for clone #23. Conclusion: The results will be useful for modeling GVL and generation TCR transgenic mouse.

• 한국발생생물학회지:발생과생식
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• 제27권4호
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• pp.213-220
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• 2023

Previously, we showed that a chronic-low-dose nonylphenol (NP) exposure resulted in histological changes with sexually dimorphic pattern in rat adrenal glands. We hypothesized that such structural changes are closely related to the hormonal secretory patterns. To test this hypothesis, we developed the short-term adrenal incubation method, and measured the levels of catecholamines and cortical steroids using the high-performance liquid chromatography with electrochemical detection (HPLC-ECD) and specific enzyme-linked immunosorbent assay, respectively. The norepinephrine (NE) levels in media from NP-treated female adrenal, except 100 pM NP, were significantly increased [control (CTL) vs 1 nM NP, p<0.001; vs 10 nM NP, p<0.05; vs 100 nM NP, p<0.001; vs 1 µM NP, p<0.01]. The NE secretion from male adrenal was higher when treated with 100 nM and 1 µM NP (CTL vs 100 nM NP, p<0.05; vs 1 µM NP, p<0.05, respectively). The aldosterone level in the female adrenal media treated with 100 pM NP was significantly decreased, on the other hand, that of media treated with 10 nM NP was significantly increased (CTL vs 100 pM NP, p<0.05; vs 10 nM NP, p<0.01). In male adrenal media, the aldosterone levels of 10 nM, 100 nM and 1 µM NP-treated media were significantly declined (CTL vs 10 nM NP, p<0.001; vs 100 nM NP, p<0.001; vs 1 µM NP, p<0.001). These results showed the NP treatment altered secretory pattern of aldosterone from adrenals of both sexes, showing sexual dimorphism. It may be helpful for understanding possible adrenal pathophysiology, and endocrine disrupting chemicals-related sexually dimorphic phenomena in adrenals.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2010년도 춘계학술대회 초록집
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• pp.107.2-107.2
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• 2010

고온건식탈황기술은 고온고압에서 석탄가스에 함유된 황화합물을 제거하는 기술로 석탄가스화에 의해 생성된 고온의 석탄가스의 열손실을 최소화하여 열효율이 높은 기술이다. 본 연구에서는 석탄으로부터 합성원유를 생산하는 0.3 배럴/일 규모 석탄액화(CTL)공정의 연계운전을 통하여 건식탈황공정의 성능을 평가하였다. 0.3 배럴/일 규모 석탄액화공정은 석탄가스화기, 건식탈황공정, 액화공정으로 구성되어 있으며 30 atm의 고압에서 운전된다. 건식탈황공정은 석탄가스화기와 액화공정 사이에 위치하여 석탄가스화로부터 생성된 석탄가스에 함유된 황화합물을 아연계 건식탈황제에 의해 제거한 후 액화반응기로 공급하여 황화합물에 의한 촉매의 피독을 막아주는 역할을 수행한다. 본 연구에서는 기존에 개발된 두 개의 기포유동층 반응기로 구성된 탈황장치를 30 atm에서 운전이 가능하도록 수정/보완하여 실제 운전압력인 30 atm의 고압에서 연속운전을 수행하였다. 실험 결과 탈황효율은 99% 이상이며 탈황반응기 출구 황화합물의 농도는 1 ppmv 이하로 유지하였다.

• Journal of Korean Biological Nursing Science
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• 제14권2호
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• pp.122-128
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• 2012

Purpose: This study was aim to examine the effect of extract from several herbs on wound treatment which are grown naturally in Namwon province. Methods: Adult male Sprague-Dawley rats were assigned to 6 of 2 group: control group (C, n=6), experimental group (E, n=6). Rats in E group had 3 sessions (everyday) for 4 days or 8 days of the extract treatment which was composed of German chemomile, Teatree and Lavender (CTL). Results: CTL extract on wound-induced mice showed significantly the increase of wound treatment rate (WTR), epidermal gap (EP) and collagen density (CD), compared with control. Conclusion: CTL extract may be a putative resources for cure or treatment of wound.

• Journal of Computing Science and Engineering
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• 제9권1호
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• pp.9-19
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• 2015

The most important challenge in the region-based abstraction method as an approach to compute the state space of time Petri Nets (TPNs) for model checking is that the method results in a huge number of regions, causing a state explosion problem. Thus, region-based abstraction methods are not appropriate for use in developing practical tools. To address this limitation, this paper applies a modification to the basic region abstraction method to be used specially for computing the state space of TPN models, so that the number of regions becomes smaller than that of the situations in which the current methods are applied. The proposed approach is based on the special features of TPN that helps us to construct suitable and small region graphs that preserve the time properties of TPN. To achieve this, we use TPN-TCTL as a timed extension of CTL for specifying a subset of properties in TPN models. Then, for model checking TPN-TCTL properties on TPN models, CTL model checking is used on TPN models by translating TPN-TCTL to the equivalent CTL. Finally, we compare our proposed method with the current region-based abstraction methods proposed for TPN models in terms of the size of the resulting region graph.

• 생약학회지
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• 제22권4호
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• pp.233-239
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• 1991

Phellinus linteus was examined for its anticancer activity using an animal model. Water extract of Phellinus linteus was prepared from artificially cultivated mycelia. Neither toxicity nor abnormal changes of hematological parameters were observed in the rat given orally with high doses of drug extract for 15 days. ICR mice were transplanted with Sarcoma-180 tumor cells intraperitoneally and drug extract was daily given to the mice from 1 day after tumer transplantation for 3 weeks. Administration of drug extract significantly prolonged the survival duration of Sarcoma 180-transplanted mice. For the better understanding of the anticancer activity, we have examined the effect of the drug extract administration on various killer cell functions, such as natural killer(NK) cells, cytotoxic T-lymphocytes (CTL) and macrophages which have been known to be main effector cells in immune responses against tumors. The results from the 4 hr $^{51}Cr-release$ assay have shown that the drug extract augments mouse NK cell activity but neither CTL nor macrophages. It is possible, then, that the anticancer activity of the Phellinus linteus may be associated with augmentation of NK cell function in the cancerated hosts.

• 사상체질의학회지
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• 제29권2호
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• pp.154-173
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• 2017

Objectives This study was conducted to evaluate the effects of Yeoldahanso-tang on obesity in rats induced by high fat diet experimentally. Methods The experiment was conducted with rats divided into 5 groups. Rats were evaluated for change of weight, hematologic and serum biochemical parameters. Results Yeoldahanso-tang group showed significant reductions in FER, body weight, adipose tissue weight and size. The level of Creatinine, glucose, ALP, T-cholesterol, Triglyceride, LDL-cholesterol, Leptin and IGF-1 of Yeoldahanso-tang group was significantly lower than those in HFD-CTL group. The level of HDL-cholesterol, free fatty acid and Adiponectin in Yeoldahanso-tang group was significantly higher than those in HFD-CTL group. As compared with HFD-CTL group, AMPK-${\alpha}1$, UCP2 and adiponectin mRNA in liver of Yeoldahanso-tang group were significantly increased and AP2/FABP4, AMPK-${\alpha}2$ and PPAR-${\gamma}$ mRNA in liver of Yeoldahanso-tang group was significantly decreased. Conclusion These results suggest that Yeoldahanso-tang has inhibitory effects on obesity in high fat diet induced obese mice.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2008년도 춘계 학술발표회 제20권1호
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• pp.857-860
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• 2008

본 연구는 잠실제2롯데월드에 적용된 50, 60, 70MPa 콘크리트 강도에 대한 크리프 및 건조수축 특성 파악을 위한 재료연구의 일환으로 진행된 콘크리트 테스트 프로그램의 준비 단계에 대한 일련의 체계화된 과정을 나타내고 있다. 콘크리트 테스트 프로그램의 준비단계는 국내와 국외에서 진행되었다. S사 실험실에서 진행된 국내 진행일정은 총 5일간 진행되었으며 그 진행과정은 공시체 제작, 몰드탈형, 공시체 마킹, 수중양생, 포장, 및 운송으로 이루어져 있다. 국외에서는 PCA(Portland Cement Association)산하 CTL사에서 진행되고 있으며 테스트 항목은 탄성계수 측정테스트(정탄성 & 동탄성), 압축강도 테스트, 크리프 건조수축 테스트, 및 할렬인장강도 테스트가 진행되고 있다. 크리프 및 건조수축 실험의 경우에는 하중 재령일에 따라 총 18개월간 테스트가 진행될 예정이다.

• Coupled systems mechanics
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• 제3권1호
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• pp.1-25
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• 2014

In this work, we present the theoretical formulation, operator split solution procedure and partitioned software development for the coupled thermomechanical systems. We consider the general case with nonlinear evolution for each sub-system (either mechanical or thermal) with dedicated time integration scheme for each sub-system. We provide the condition that guarantees the stability of such an operator split solution procedure for fully nonlinear evolution of coupled thermomechanical system. We show that the proposed solution procedure can accommodate different evolution time-scale for different sub-systems, and allow for different time steps for the corresponding integration scheme. We also show that such an approach is perfectly suitable for parallel computations. Several numerical simulations are presented in order to illustrate very satisfying performance of the proposed solution procedure and confirm the theoretical speed-up of parallel computations, which follow from the adequate choice of the time step for each sub-problem. This work confirms that one can make the most appropriate selection of the time step with respect to the characteristic time-scale, carry out the separate computations for each sub-system, and then enforce the coupling to preserve the stability of the operator split computations. The software development strategy of direct linking the (existing) codes for each sub-system via Component Template Library (CTL) is shown to be perfectly suitable for the proposed approach.