• 대한약침학회지
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• 제8권1호
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• pp.31-40
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• 2005

Objectives : It has long been known about the osteogenic effect of CTF-HAS on bone tissues. However, it has not been determined the effect of CTF-HAS on cancer cells. The purpose of this study is to screen the CTF-HAS mediated differentially expressed genes in cancer cells such as SNU484 gastric cancer cell lines. Oligonucleotide microarray approach were employed to screen the differential expression genes. Methods : CTF-HAS was prepared by boiling and stored at $-70^{\circ}C$ until use. Cells were treated with various concentrations of CTF-HAS(0.1, 0.5, 1.5, 10, 20mg/ml) for 24 h. Cytotoxicity was tested by MTT assay. To screen the differentially expressed genes in cancer cells, cells were treated with 1.5mg/ml of CTF-HAS. For oligonucleotide microarry assay, total RNA was used for gene expression analysis using oligonucleotide genechip (Human genome U133 Plus 2.0., Affimatrix Co.). Results : It has no cytotoxic effects on HepG2 cells in all concentration (0.1, 0.5, 1.5, 10,20mg/ml). More than twofold up-regulated genes were 5 genes. The number of more than twofold down-regulated genes was 10. Discussion : This study showed the screening of CTF-HAS mediated differentially regulated genes using combined approaches of oligonucleotide microarray. The screened genes will be used for the better understanding in therapeutic effect of CTF-HAS on cancer field.

• 대한약침학회지
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• 제8권2호
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• pp.23-28
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• 2005

Objective : It has long been known about the osteogenic effect of CTF-HAS on bone tissues. However, it has not been determined the effect of CTF-HAS on cancer cells. The purpose of this study is to screen the CTF-HAS mediated differentially expressed genes in cancer cells such as HepG2 hepatoma cells lines. Methods : CTF-HAS was prepared by boiling and stored at $-70^{\circ}C$ until use. For proteomic analysis, total protein was analyzed by 2D gel electrophoresis and Q-TOF mass spectrometer. Results : In proteomic analysis, three spots were identified by 2D-gel electrophoresis and Q-TOF analysis. One down-regulated protein was heat shock 70kDa protein 5 and up-regulated proteins were chaperonin and 2-phospho -pyruvate-hydratase ${\alpha}-enolase$ by 1.5mg/ml of CTF-HAS. Discussion : Proteomic analysis approach were performed to screen the differential expression genes. The screened genes will be used for the better understanding in therapeutic effect of CTF-HAS on cancer field.

• 전기전자학회논문지
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• 제25권4호
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• pp.774-777
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• 2021

본 논문은 Charge Trap Flash using Ferroelectric(CTF-F) 구조를 가진 3D NAND Flash Memory gate controllability에 대해 분석했다. Ferroelectric 물질인 HfO₂는 polarization 이외에도 high-k 라는 특징을 가진다. 이러한 특징으로 인해 CTF-F 구조에서 gate controllability가 증가하고 Bit Line(BL)에서 on/off 전류특성이 향상된다. Simulation 결과 CTF-F 구조에서 String Select Line(SSL)과 Ground Select Line(GSL)의 채널길이는 100 nm로 기존 CTF 구조에 비해 33% 감소했지만 거의 동일한 off current 특성을 확인했다. 또한 program operation에서 channel에 inversion layer가 더 강하게 형성되어 BL을 통한 전류가 약 2배 증가한 것을 확인했다.

• Nuclear Engineering and Technology
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• 제52권4호
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• pp.661-673
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• 2020

The quarter-core simulation of BEAVRS Cycle 2 depletion benchmark has been conducted using the MCS/CTF coupling system. MCS/CTF is a cycle-wise Picard iteration based inner-coupling code system, which couples sub-channel T/H (thermal/hydraulic) code CTF as a T/H solver in Monte Carlo neutron transport code MCS. This coupling code system has been previously applied in the BEAVRS benchmark Cycle 1 full-core simulation. The Cycle 2 depletion has been performed with T/H feedback based on the spent fuel materials composition pre-generated by the Cycle 1 depletion simulation using refueling capability of MCS code. Meanwhile, the MCS internal one-dimension T/H solver (MCS/TH1D) has been also applied in the simulation as the reference. In this paper, an analysis of the detailed criticality boron concentration and the axially integrated assembly-wise detector signals will be presented and compared with measured data based on the real operating physical conditions. Moreover, the MCS/CTF simulated results for neutronics and T/H parameters will be also compared to MCS/TH1D to figure out their difference, which proves the practical application of MCS into the BEAVRS benchmark two-cycle depletion simulations.

• Journal of Acupuncture Research
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• 제22권3호
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• pp.215-225
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• 2005

홍화자약침액(紅花子藥鍼液)의 항암효능(抗癌效能)을 밝히고자 간암세포주(肝癌細胞柱)에 최신 oligonucleotide chip assay 법을 통하여 대양(大量)의 유전자(遺傳子) 발현(發顯)을 분석(分析)한 결과(結果) 다음과 같은 결론(結論)을 얻었다. 1. MTT 분석(分析)에서 간암(肝癌) 및 위암세포주(胃癌細胞柱)는 홍화자약침액(紅花子藥鍼液) 1.5, 10, 20m/$m{\ell}$에서 대조군(對照群)에 비해 유의(有意)한 세포활성(細胞活性) 감소(減少)를 보였다. 2. 간암세포주(肝癌細胞柱)에 홍화자약침액(紅花子藥鍼液) 처치(處置) 시(時) 대조군(對照群)에 비해 발현(發顯)이 2배 이상 증가(增加)된 유전자(遺傳子)는 UCIA PMARARA fusion protein, human oral cancer candidate gene mRNA, EPPIN-3 등 19개였다. 3. 간암세포주(肝癌細胞柱)에 홍화자약침액(紅花子藥鍼液) 처치(處置) 시 (時)대조군(對照群)에 비해 유전자(遺傳子)의 발현(發顯)이 2배 이상 감소(減少)된 것은 BTF3, MMP11, paxillin, villinn 등 13개였다. 이상과 같이 홍화자약침액(紅花子藥鍼液)에 대한 간암세포주(肝癌細胞柱)의 유전자(遺傳子) 발현(發顯)을 oligonucleotide 분석(分析)으로 대량(大量) 검소(儉素)할 수 있었고, 심한 발현(發顯) 차이(差異)를 나타내는 각 유전자(遺傳子)는 암화(癌化) 과정(過程)이나, 홍화자약침액(紅花子藥鍼液)에 반응하는 유전자(遺傳子)로 치료제 개발을 위한 기초 자료로 활용할 수 있을 것으로 사료(思料)된다.

• 설비공학논문집
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• 제11권5호
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• pp.684-691
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• 1999

A load calculation program based on TFM(Transfer function method) has been proposed in this study. The validity of the current method has been verified by comparing heat gain calculation by TRF(Thermal response factor) with that by CTF(Conduction transfer function) adopted in ASHRAE. In addition, it seems that the CTF coefficients given in ASHRAE tables have somewhat ambiguity The load calculation program developed in the current study has been employed to calculate cooling load from the exterior walls and roof of example 6 in the ASHRAE. The results are found in good agreement.

• 한국화재소방학회논문지
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• 제26권2호
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• pp.69-74
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• 2012

적외선 파장영역의 높은 연기투과율에 기초한 열화상기술은 화재안전분야에서 효과적인 첨단기술 중의 하나로 인식되고 있다. 본 연구는 주변열적조건에 따른 열영상 포화특성을 파악하고 대조비변환함수(CTF)를 이용하여 영상의 수준을 평가하고자 한다. 본 실험에서 수행된 조건에 대해 BST 센서를 적용한 열화상 카메라의 경우 영상포화가 발생하지 않았으며 영상수준이 배경과 목표물의 온도차에 비례하는 것으로 나타났다. 본 연구는 열화상기술의 신뢰성을 확보하고 기술개발을 위한 기초자료로 활용될 수 있다.

• International Journal of Computer Science & Network Security
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• 제23권1호
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• pp.96-102
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• 2023

In this paper, we proposed Channel Transfer Function estimation based on Delay and Doppler Profile for underwater acoustic OFDM communication system. It improved the estimation accuracy of the channel transfer function by linear time interpolation the change of Scattered Pilot (SP) insertion frequency in the time direction and the time by Delay and Doppler profile that analyzes the multipath situation of the channel investigated the performance of interpolation by simulation and report it. Previous works is inserted SP every 4 OFDM. It was effective under the environment without multipath, but it has observed that the effect of CTF compensation has been lowered in multipath channel condition. In addition to be better when inserted SP every 2 OFDM. But the amount of sending data will be decrease. Therefore, we conducted research to improve 4 OFDM with new interpolator. A computer simulation was performed as a comparison of SP inserted every 4 OFDM, SP inserted every 2 OFDM, and 4 OFDM with new interpolator. the performance of the proposed system is overwhelmingly improved, and the performance is slightly improved even 64 QAM.

• Journal of Microbiology and Biotechnology
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• 제25권10호
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• pp.1702-1708
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• 2015

We have developed a new shuttle plasmid, designated as pLK1-MCS that can replicate in both Clostridium acetobutylicum and Escherichia coli, by combining the pUB110 and pUC19 plasmids. Plasmid pLK1-MCS replicated more stably than previously reported plasmids containing either the pIM13 or the pAMβ1 replicon in the absence of antibiotic selective pressure. The transfer frequency of pLK1-MCS into C. acetobutylicum was similar to the transfer frequency of other shuttle plasmids. We complemented C. acetobutylicum ML1 (that does not produce solvents such as acetone, butanol, and ethanol owing to loss of the megaplasmid pSOL1 harboring the adhE1-ctfAB-adc operon) by introducing pLK1-MCS carrying the adhE1-ctfAB-adc operon into C. acetobutylicum ML1. The transformed cells were able to resume anaerobic solvent production, indicating that the new shuttle plasmid has the potential for practical use in microbial biotechnology.

• International Journal of Computer Science & Network Security
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• 제23권10호
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• pp.1-10
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• 2023

This paper proposes a method to extend Inter-Carrier Interference (ICI) canceling Orthogonal Frequency Division Multiplexing (OFDM) receivers for 5G mobile systems to spatial multiplexing 2×2 MIMO (Multiple Input Multiple Output) systems to support high-speed ground transportation services by linear motor cars traveling at 500 km/h. In Japan, linear-motor high-speed ground transportation service is scheduled to begin in 2027. To expand the coverage area of base stations, 5G mobile systems in high-speed moving trains will have multiple base station antennas transmitting the same downlink (DL) signal, forming an expanded cell size along the train rails. 5G terminals in a fast-moving train can cause the forward and backward antenna signals to be Doppler-shifted in opposite directions, so the receiver in the train may have trouble estimating the exact channel transfer function (CTF) for demodulation. A receiver in such high-speed train sees the transmission channel which is composed of multiple Doppler-shifted propagation paths. Then, a loss of sub-carrier orthogonality due to Doppler-spread channels causes ICI. The ICI Canceller is realized by the following three steps. First, using the Demodulation Reference Symbol (DMRS) pilot signals, it analyzes three parameters such as attenuation, relative delay, and Doppler-shift of each multi-path component. Secondly, based on the sets of three parameters, Channel Transfer Function (CTF) of sender sub-carrier number n to receiver sub-carrier number l is generated. In case of n≠l, the CTF corresponds to ICI factor. Thirdly, since ICI factor is obtained, by applying ICI reverse operation by Multi-Tap Equalizer, ICI canceling can be realized. ICI canceling performance has been simulated assuming severe channel condition such as 500 km/h, 8 path reverse Doppler Shift for QPSK, 16QAM, 64QAM and 256QAM modulations. In particular, 2×2MIMO QPSK and 16QAM modulation schemes, BER (Bit Error Rate) improvement was observed when the number of taps in the multi-tap equalizer was set to 31 or more taps, at a moving speed of 500 km/h and in an 8-pass reverse doppler shift environment.