• Journal of Microbiology
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• 제43권6호
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• pp.516-522
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• 2005

The phosphorylation of C-terminal domain (CTD) of Rpb1p, the largest subunit of RNA polymerase II plays an important role in transcription and the coupling of various cellular events to transcription. In this study, its role in DNA damage response is closely examined in Saccharomyces cerevisiae, focusing specifically on several transcription factors that mediate or respond to the phosphorylation of the CTD. CTDK-1, the pol II CTD kinase, FCP1, the CTD phosphatase, ESS1, the CTD phosphorylation dependent cis-trans isomerase, and RSP5, the phosphorylation dependent pol II ubiquitinating enzyme, were chosen for the study. We determined that the CTD phosphorylation of CTD, which occurred predominantly at serine 2 within a heptapeptide repeat, was enhanced in response to a variety of sources of DNA damage. This modification was shown to be mediated by CTDK-1. Although mutations in ESS1 or FCP1 caused cells to become quite sensitive to DNA damage, the characteristic pattern of CTD phosphorylation remained unaltered, thereby implying that ESS1 and FCP1 play roles downstream of CTD phosphorylation in response to DNA damage. Our data suggest that the location or extent of CTD phosphorylation might be altered in response to DNA damage, and that the modified CTD, ESS1, and FCP1 all contribute to cellular survival in such conditions.

• 기본간호학회지
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• 제19권3호
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• pp.334-342
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• 2012

Purpose: This descriptive study was done to identify the academic achievement, self-directed learning (SDL), and critical thinking disposition (CTD) of nursing students according to their learning styles. Method: The participants were 240 nursing students. Data were collected using structured questionnaires which included Kolb's Learning Style Inventory, Academic Achievement in Fundamental Nursing and Health Assessment, Self Directed Learning Readiness Scale, and California Critical Thinking Disposition Inventory. Data were analyzed using ${\chi}^2$ test, ANOVA, Pearson' correlation coefficients, and Spearman rank correlation coefficient. Results: One third of respondents were shown to be Convergers in their learning style (33.3%). The Academic Achievement of students who were Convergers was significantly higher than those who were Divergers or Accommodators (F=5.95, p=.001). The SDL and CTD of students who were Convergers were significantly higher than Divergers and Assimilators (F=9.67, p<.001 and F=8.42, p<.001). No significant correlations were found between Academic Achievement and SDL or CTD, but a statistically significant positive correlation was found between SDL and CTD (r=.68, p<.001). Conclusion: The findings of this study indicate that learning style influences academic achievement, SDL and CTD.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제34권1호
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• pp.37-44
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• 2023

Objectives: Tic disorders are highly heritable; however, growing evidence suggests that environmental factors play a significant role in their pathogenesis. Studies on these factors have been inconsistent, with conflicting results. Therefore, this study aimed to examine the associations of pre- and perinatal exposure to Tourette syndrome (TS) or chronic tic disorders (CTD) in Korean school-aged children. Methods: This case-control study used data from a large prospective cohort study. The primary outcome was TS/CTD diagnosis according to the Diagnostic and Statistical Manual of Mental Disorders, 5th edition (DSM-5) criteria and Kiddie-Schedule for Affective Disorders and Schizophrenia-Present and Lifetime Version-Korean Version. Demographic, pre-, and perinatal information was obtained from the maternal questionnaires. Data between the TS/CTD and control groups were compared using the chi-squared or Student's t-test, as appropriate. Two-step logistic regression analyses were used to test the association between TS/CTD and pre- and perinatal risk factors. Results: We included of 223 children (78 with TS/CTD and 145 controls). Significant differences in the demographic data between the two groups were observed. The male sex ratio, mean parental age, parental final education level, and family history of tics were included as confounders. In the final adjusted multivariable model, TS/CTD was significantly associated with antiemetic exposure during pregnancy (odds ratio [OR]=16.61, 95% confidence interval [CI] 1.49-185.22, p=0.02) and medically assisted reproduction (OR=7.89, 95% CI 2.28-27.28, p=0.01). Conclusion: Antiemetic exposure and medically assisted reproduction are significantly associated with the risk of TS/CTD. These results should be replicated in future prospective and gene-by-environment studies.

• 한국융합학회논문지
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• 제10권3호
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• pp.373-382
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• 2019

본 연구는 일개 대학교 간호학과 3개 학년 5개 전공교과목에 기억력과 실용능력 증진을 위한 창의적 교수법 일부를 적용하여 비판적 사고성향, 문제해결능력, 셀프리더십에 대한 영향 여부를 조사하고자 실시되었다. 자료수집은 2017년 8월 1일부터 12월 31일까지 수행되었으며 창의적교수법 적용 전후 설문조사를 시행하였다. 적용 결과 학년별로 1개 교과목에 다양한 교수법을 적용한 1학년의 경우에는 비판적 사고성향, 문제해결과정, 셀프리더십에 차이가 없었으나, 2개 교과목에서 유사한 교수법을 반복하여 적용한 2학년과 3학년의 경우 비판적 사고성향은 6.8점(p<0.001), 문제해결과정은 7.5~7.9점(p<0.001), 셀프리더십은 2.9~3.3점(p<0.001) 향상되었다. 학생들의 핵심역량을 향상시키기 위해 하나의 교과목에 여러 가지 교수법을 적용하는 것보다 1~2가지 방법을 여러 교과목에 반복적으로 적용하는 것이 보다 효과적임이 확인되었다. 창의적 교수법은 적성과 흥미에 따라 전공을 선택한 그룹에서 보다 효과적이었으므로 추후 전공에 대한 흥미를 촉진 시킬 수 있는 다양한 교수학습 전략을 개발할 필요가 있다.

• Bulletin of the Korean Chemical Society
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• 제30권5호
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• pp.1039-1042
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• 2009

RNA polymerase II C-terminal domain phosphatase 1 containing ubiquitin like domain (UBLCP1) has been identified as a regulatory molecule of RNA polymerase II. UBLCP1 consists of ubiquitin like domain (UBL) and phosphatase domain homologous with UDP and CTD phosphatase. UBLCP1 was cloned into the E.coli expression vectors, pET32a and pGEX 4T-1 with TEV protease cleavage site and purified using both affinity and gel-filtration chromatography. Domains of UBLCP1 protein were successfully purified as 7 mg/500 mL (UBLCP1, 36.78 KDa), 32 mg/500 mL (UBL, 9 KDa) and 8 mg/500 mL (phosphatase domain, 25 KDa) yielded in LB medium, respectively. Isotope-labeled samples including triple-labeled ($^2H/^{15}N/^{13}C$) UBLCP1 were also prepared for hetero-nuclear NMR experiments. $^{15}N-^{1}H$ 2D-HSQC spectra of UBLCP1 suggest that both UBL and phosphatase domain are properly folded and structurally independent each other. These data will promise us further structural investigation of UBLCP1 by NMR spectroscopy and/or X-ray crystallography.

• 간호행정학회지
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• 제21권2호
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• pp.143-153
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• 2015

Purpose: The aim of this study was to describe competency in evidence-based practice (EBP) among clinical nurses and to explore critical thinking disposition (CTD) and organizational culture (OC) as possible predictors for competency in EBP. Also this study was conducted to identify baseline data for purpose of developing evidence-based OC to ensure safety of patients and to improve quality of nursing services in clinical setting. Methods: With a descriptive correlation study design, a competency in EBP questionnaire was administered to a convenience sample of nurses (N=393) from a single general hospital in Gyeonggi Province. Results: The overall mean score for competency in EBP was $3.03{\pm}0.53$. Factors influencing competency in EBP were education (${\beta}=0.23$, p<.001), attendance at lectures on EBP (${\beta}=0.18$, p<.001), intellectual eagerness & healthy skepticism (${\beta}=0.27$, p<.001) among CTD, and hierarchy-oriented OC (${\beta}=0.14$, p=.005), which explained about 35% of the total variance. Conclusion: Critical thinking and competency in EBP should be part of nursing education especially for undergraduate students as well as strengthening clinical practice by activation of EBP. The results of this study that hierarchy-oriented OC, is a significant predictor for competency in EBP is difficult to accept. Its seemed to require further exploration.

• 한국광학회:학술대회논문집
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• 한국광학회 2009년도 동계학술발표회 논문집
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• pp.133-134
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• 2009

Cold temperature development (CTD) of electron beam (EB) patterned resists and subsequent dry etching were investigated for fabrication of nano-patterned Niobium (Nb). Bulky Nb fims on GaAs substrates were deposited with EB evaporation. Line patterns on Nb cathode were fabricated by EB patterning and reactive ion etching (RIE). Size deviations of nano-sized line patterns from CAD designed patterns are dependent on the EB total exposure, but it can be improved by CTD of EB-exposed resist. Line patterns of 10 to 300 nm widths of EB-exposed resist patterns were drawn under various exposure conditions of $0.2{\mu}s$/dot (total 240,000 dot) with a constant current (50 pA). Compared with room temperature development (RTD), the CTD improves pattern resolution due to the suppression of backscattering effect. RIE with $CF_4$ was performed for formation of several nano-sized line patterns on Nb. Each EB-resist patterned samples with RTDs and CTDs were etched with two different $CF_4$ gas pressures of 5 Pa. Nb etching rate increases while GaAs (or ZEP) etching rate decreases as the chamber pressure increases. This different dependent of the etching rate on the $CF_4$ pressure between Nb and GaAs (or ZEP) has a significant meaning because selective etching of nano-sized Nb line patterns is possible without etching of the underlying active layer.

• Communications for Statistical Applications and Methods
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• 제13권3호
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• pp.513-524
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• 2006

In this paper, we introduce a new notions of conditionally weak dependence and we study their properties, preservation of the conditionally weak independent and positive and negative quadrant dependent(CWQD) property under mixtures, limits, closure under convex combinations, and their interrelationships. Furthermore, we extend multivariate stochastic dependence to stronger conditions of dependence.

• 지구물리와물리탐사
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• 제11권3호
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• pp.167-176
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• 2008

태평양 해양 지각판과 인도-호주 대륙 지각판간 섭입작용에 의해 형성된 남태평양 라우분지는 활동성 후열도분지로서 해저열수광상이 부존할 가능성이 매우 높은 지역이다. 한국해양연구원은 라우분지를 대상으로 다중음향측심장비(EM120)을 이용하여 정밀지형조사를 실시하여 열수활동이 활발할 것으로 예측되는 해저 지각 확장축 주변지역 (FRSC)과 해저화산 지역(MTJ)을 선별하였다. 또한, 표층 및 심해견인 자력탐사결과를 토대로 저 자기이상 현상을 나타내는 열수광체 지역을 선정하였다. 표층 및 심해 견인 자력탐사 결과 해령에서 주로 나타나는 Central Anomaly Magnetization High(CAMH)가 FRSC-2 지역에서 관측되었으며, MTJ-1 지역에서는 열수분출작용으로 추정되는 저자화이상이 발견되었다. CTD 시스템을 이용하여 열수 플룸 추적자인 투명도, 수소이온(pH), 미생물생체량(ATP), 메탄$(CH_4)$농도를 실시간으로 측정한 결과 FRSC-2와 MTJ-1 지역은 현재 매우 활발한 화산 활동이 진행되고 있음을 알 수 있었다. 이 지역에서 채취한 열수분출공과 기반암 시료는 이 지역에서 열수활동이 진행되었거나 진행되고 있으며, 실제로 열수 광체가 부존하고 있음을 확인할 수 있었다. 첨단 해저면 영상장비를 사용하지 않고도, 전통적인 해양 지구물리탐사 방법이 해저열수광상의 탐지에 비용 효과적인 탐사방법임을 알 수 있었다.

• 한국해양학회지:바다
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• 제5권4호
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• pp.363-373
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• 2000

남서태평양 열수해역의 화학적 환경특성을 이해하기 위하여 수온, 염분, 투명도, pH, 영양염 및 황화수소 등을 분석하였다. 시료는 마누스분지의 12개 정점에서 CTD를 이용하여 채취하였다. 열수작용은 해수 및 암석간의 순환작용에 의해 이들의 물리, 화학적 특성을 변화시키고, 주변의 해수와는 특성이 다른 열수가 plume을 형성하여 해양으로 공급된다. 본 연구에서는 남서태평양 PACMANUS 해역과 Susu Knolls 해역에서 투명도 및 황화수소를 이용하여 열수작용에 의한 plume을 확인하였다 특히, 열수환경을 지시하는 지화학적 추적자인 황화수소는 0${\sim}$3.31 ${\mu}$M 범위와 평균 0.63${\mu}$M을 보였다. 열수작용에 의한 plume의 높이, 분출량 및 활동도는 저층해류 순환 등의 영향을 받으며 수층의 등밀도면을 따라 주변 해수로 확산된다. 결과적으로 연구 해역에서의 황화수소 농도 이상치는 열수작용에 의한 영향으로 판단되고, 따라서 PACMANUS 해역의 남북방향에 가장 큰 공급원이 있을 것으로 보인다.