• Parasites, Hosts and Diseases
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• 제39권1호
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• pp.57-66
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• 2001

In the course of immunoscreening of Clonorchis sinensis cDNA library, a cDNA CsRP12 containing a tandem repeat was isolated. The cDNA CsRP 12 encodes two putative peptides of open reading frames (ORFs) 1 and 2 (CsRP12-1 and -2). The repetitive region is composed of 15 repeats of 10 amino acids. Of the two putative peptides, CsRP12-1 was proline-rich and found to have homologues in several organisms. Recombinant proteins of the putative peptides were bacterially produced and purified by an affinity chromatography Recombinant CsRP12-1 protein was recognized by sera of clonorchiasis patients and experimental rabbits, but recombinant CsRP 12-2 was not. One of the putative peptide, CsRP12-1, is designated CsPRA, proline-rich antigen of C. sinensis. Both the C-termini of CsRP12-1 and -2 were bacterially produced and analysed to show no antigenicity. Recombinant CsPRA protein showed high sensitivity and specificity. In experimental rabbits, IgG antibodies to CsPRA was produced between 4 and 8 weeks after the infection and decreased thereafter over one you. These results indicate that CsPRA is equivalent to a natural protein and a useful antigenic protein for serodiagnosis of human clonorchiasis.

• 한국작물학회지
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• 제52권4호
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• pp.458-468
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• 2007

To tolerate environmentally adverse conditions such as cold, drought, and salinity, plants often synthesize and accumulate proline in cells as compatible osmolytes. ${\Delta}^1$-Pyrroline-5-carboxylate synthetase(P5CS) catalyzes the rate-limiting step of proline biosynthesis from glutamate. Two complete genes, MtP5CS1 and MtP5CS2, were isolated from the model legume Medicago truncatula by cDNA cloning and bacterial artificial chromosome library screening. Nucleotide sequence analysis showed that both genes consisted of 20 exons and 19 introns. Alignment of the predicted amino acid sequences revealed high similarities with P5CS proteins from other plant species. The two MtP5CS genes were expressed in response to high salt and low temperature treatments. Semi-quantitative reverse transcription-polymerase chain reaction showed that MtP5CS1 was expressed earlier than MtP5CS2, indicating differential regulation of the two genes. To evaluate the reverse genetic effects of nucleotide changes on MtP5CS function, a Targeting Induced Local Lesions in Genomes approach was taken. Three mutants each were isolated for MtP5CS1 and MtP5CS2, of which a P5CS2 nonsense mutant carrying a codon change from arginine to stop was expected to bring translation to premature termination. These provide a valuable genetic resource with which to determine the function of the P5CS genes in environmental stress responses of legume crops.

• 한국게임학회 논문지
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• 제17권6호
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• pp.39-50
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• 2017

교육 게이미피케이션은 컴퓨터 과학(Computer Science, CS)분야에 많이 활용되고 있다. 본 연구는 CS 교육 게이미피케이션 선행 연구를 분석하고, 이를 통해 국내 CS 교육의 방향을 제언하는 것이 목적이다. 연구 진행을 위해서 경험적연구 방법을 적용했다. 키워드 검색을 통해 1220개의 선행연구를 수집했고, 그중에서 1차, 2차 추출과정을 통해 55개로 압축했다. 선행 연구 분석 결과 년도별 발표 현황, 적용 교과, 교육 대상, 게이미피케이션 요소, 결과 및 한계점에 대해 해석했다. 추후 연구에서 국내 CS 교육 게이미피케이션 사례를 분석하고, 본 연구와 비교분석할 예정이다. 그리고 국내 CS 교육 게이미피케이션의 발전 방향에 대해서 제언할 것이다.

• Journal of Periodontal and Implant Science
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• 제28권1호
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• pp.71-86
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• 1998

The purpose of this study was to evaluate clinically and histopathologically the effects to the periodontal tissue in rats after Cyclosporin A (CsA) administration and to determine whether there is a relationship between dosage of CsA or blood CsA level and the seventy of gingival overgrowth in rats. Twenty 6-week-old Sprauge-Dawley Fats were randomized into 4groups. The control group received olive oil only and the test group received daily CsA in olive oil via gastric feeding for 6weeks at a 3,10, and 30mg/kg. Rats were weighed to evaluate the systemic effect of drug and stone models were made from alginate impressions of upper and lover anterior region at 2 week interval. On completion of offal CsA administration, blood were collected and blood CsA levels were quantitated by TDxFLx analyzer. Rats were sacrificed an6 their upper and lower jaws were removed together with the surrounding gingiva and soft tissue for light microscopic examination. The results were as follows : 1. The weight gain of GsA-treated rats was much less than of the control group and central incisors were gradually displaced and separated in the test groups. 2. The extensive fibrovascular proliferation and scattered inflammatoy infiltrates in an edematous stroma were observed in enlarged gingiva of CsA-treated rats. 3. The increase in buccolingual, mesiodistal dimension of the anterior teeth and vertical height of the interdental papilla showed dose-dependent manner in CsA-treated rats. 4. Significant positive correlation exists between blood CsA level and the severity of gingival overgrowth in anterior teeth. This result indicates that the severity of gingival enlargement in CsA treated rats is correlated with dosage of CsA administration and blood CsA level.

• 정보교육학회논문지
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• 제23권6호
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• pp.655-663
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• 2019

소프트웨어 교육에 대한 관심이 늘어감에 따라 컴퓨터과학에 대한 관심도 높아지고 있다. 컴퓨터과학은 컴퓨터와 컴퓨터시스템의 이론과 원리를 학습하는 학문으로서 이러한 컴퓨터과학의 지식과 개념은 소프트웨어 교육의 근본이라고 할 수 있다. 최근에 컴퓨터를 사용하지 않고 컴퓨터 과학의 핵심 내용을 전달하는 방법으로 컴퓨터과학 언플러그드라는 학습 방법이 전 세계적으로 수업에 많이 활용되고 있다. CS 언플러그드는 다양한 놀이 활동을 중심으로 컴퓨터과학의 원리를 배우도록 하는 학습 방법이다. 그러나 문제는 이러한 CS 언플러그드 활동이 실제 컴퓨터과학에서 배우는 핵심교과와 얼마나 연관성이 있는지에 대한 분석 없이 단순한 놀이 수업으로만 이루어지는 것이다. 따라서 본 연구에서는 컴퓨터과학의 핵심 내용과 CS 언플러그드의 다양한 활동에 들어 있는 컴퓨터과학 핵심 내용을 분석하여 비교 연구를 하고자 한다.

• 창의정보문화연구
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• 제6권2호
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• pp.77-88
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• 2020

본 연구의 목적은 CS 언플러그드 교육이 초등학교 영재학급 학생의 컴퓨팅 사고력에 미치는 영향을 관찰하고 분석하는데 있다. 이를 위해 용인시 소재 D초등학교의 5학년 영재학급 학생 17명을 대상으로 10차시의 CS 언플러그드 수업을 진행하였고, 학생들의 사전·사후 설문, 수업일지 및 학생활동지, 학생 면담 기록을 수집하여 이를 바탕으로 질적 사례연구를 수행하였으며, 사전·사후 컴퓨팅 사고력 검사를 실시하여 보조 자료로 사용하였다. 연구 결과는 다음과 같다. 첫째, CS 언플러그드 교육은 초등학교 영재학급 학생들의 컴퓨팅 사고력을 향상 시키는데 효과적인 역할을 한다. 둘째, CS 언플러그드 교육이 컴퓨팅 사고력의 모든 하위 영역에서 통계적으로 유의미한 개선을 가져오지는 못했다. 셋째, CS 언플러그드 교육은 영재 SW 교육과정의 첫 단계로 제시하기 적합하다.

• Animal Bioscience
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• 제35권11호
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• pp.1800-1807
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• 2022

Objective: This study aimed to determine the effect of different cooling sessions (CSs) as a water conservation strategy on physiological, and production responses and welfare in Holstein Friesian cows during subtropical summer in Pakistan. Methods: Twenty-one cows were subjected to three CS in a completely randomized design. The treatments were: i) eleven hours continuous cooling with sprinklers - control (CNT), ii) four CS, and iii) two CS. The CNT represented the practices of the commercial dairy farms in the area, while the other CSs were used as water reduction strategies. Each CS lasted for 1 h with a 12 min cycle (3 min water on and 9 min off) with a sprinkler flow rate of 1.25 L/min. Results: The average temperature humidity index of the shed and the outside open area were 81.9 and 82.5, respectively. The results showed that both physiological responses were highest in the 2CS group followed by the CNT and the 4CS (p = 0.001). The CNT and 4CS groups had similar milk yield (p = 0.040). The 4CS group had more lying and eating times than the CNT and 2CS groups (p = 0.000). The cortisol level in the 2CS group was 2.0 and 2.2 ㎍/dL more than the CNT and the 4CS groups, respectively (p = 0.000). Conclusion: In conclusion, the 4CS was more efficient in cooling the cows and had better welfare, as it yielded similar milk yield, and better physiological responses than the CNT despite using 90% less water.

• 품질경영학회지
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• 제50권4호
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• pp.863-876
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• 2022

Purpose: The purpose of this study is to propose the product quality satisfaction index based on multiple linear regression using customer reviews. Methods: The proposed framework is composed of four steps. First, we collect online reviews and divide it into insight phrases. The insight phrases are classified using product attribute dictionary and sentiment analysis is conducted. Second, the importance of attributes is calculated in consideration of both regression coefficient and frequency. Third, the positive rate is calculated concerning sentiment analysis result. Therefore, the quality satisfaction index is measured by the weighted sum of importance and positive rate in the last step. Results: We conduct a case study using 2-years(2020, 2021) of Samsung TV reviews to confirm the effectiveness of the proposed methodology. As a result, we found that Picture quality is the most crucial attribute in TV evaluation. The importance of Gaming and content has grown up as the positive rate has also increased. Therefore, the overall satisfaction of TV has increased in 2021 compared to 2020. Conclusion: The result of this study shows that the proposed index reveals the customer's mind efficiently and can be explained by the importance and positive rate of each attribute. By using the proposed index, companies are able to improve and the priority of improvement can be determined.

• 한국자기공명학회논문지
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• 제26권1호
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• pp.10-16
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• 2022

The phase transition temperatures of CsCoCl₃·2H₂O crystals are investigated via differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). Three endothermic peaks at temperatures of 370 K (=T_C1), 390 K (=T_C2), and 416 K (=T_C3) were observed for phase transitions from CsCoCl₃·2H₂O to CsCoCl₃·1.5H₂O, to CsCoCl₃·H₂O, and then to CsCoCl₃·0.5H₂O, respectively. In addition, the spin-lattice relaxation time T_1ρ in the rotating frame and T₁ in the laboratory frame as well as changes in chemical shifts for ¹H and ¹³³Cs near T_C1 were found to be temperature dependent. Our analyses results indicated that the changes of chemical shifts, T_1ρ, and T₁ are associated with structural phase transitions near temperature T_C1. The changes of chemical shifts, T_1ρ, and T₁ near T_C1 were associated with structural phase transitions, owing to the changes in the symmetry of the structure formed of H₂O and Cs⁺ ions. Consequently, the structural symmetry in CsCoCl₃·2H₂O crystals based on temperature is discussed by the environments of their H and Cs nuclei.

• 한국식품영양과학회지
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• 제45권5호
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• pp.651-663
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• 2016

옥수수수염, 율무, 표고버섯 그리고 사과껍질 70% 주정 추출물들(CS, JT, LE, AP)은 항산화 활성이 있었고, 그것 중에 CS가 총폴리페놀 함량, 플라보노이드 함량, DPPH 라디칼 소거작용, ABTS 라디칼 소거작용 그리고 환원력과 같은 항산화 효과가 가장 높았다. 지방분화는 CS, JT, LE, AP 그리고 옥수수수염, 율무, 표고버섯 그리고 사과껍질을 함유한 개발 빵 추출물(DB)을 각각 처리한 3T3-L1 지방세포에서 연구하였다. DB1과 DB2는 지방 전구세포 분화 억제 및 항산화 효과가 있었다. 3T3-L1 지방세포에서 중성지방 축적은 실험한 시료들(CS, JT, LE, AP) 중에서 CS가 분화된 3T3-L1 지방세포에서 TG 축적을 가장 억제하였고 3T3-L1에서 지방분화와 관련된 인자들을 조절하였다. CS는 3T3-L1 세포에서 지방구 형성과 지방세포 분화를 농도 의존적으로 억제하였다. 지방분화 동안 다양한 농도(10, 50, $100{\mu}g/mL$)에서 CS와 함께 처리한 3T3-L1 세포에서 $C/EBP{\beta}$, $PPAR{\gamma}$ 그리고 aP2 mRNA와 단백질 수준에 대한 CS의 영향력을 실험하였고, 3T3-L1 지방세포에 CS 처리는 $PPAR{\gamma}$와 aP2 mRNA 발현을 감소시켰다. CS는 역시 지방분화 중에 $C/EBP{\beta}$, $PPAR{\gamma}$와 aP2 단백질의 증가를 현저하게 저해하였다. 개발된 빵들은 CS에 의해 지방 전구세포(3T3-L1 preadipocytes) 분화 억제 효과가 있고, CS는 3T3-L1 지방세포에서 $C/EBP{\beta}$, $PPAR{\gamma}$와 aP2 신호전달경로를 저해함으로써 지방 전구세포 분화 억제 효과를 나타내었다. JT, LE와 AP는 지방 전구세포 분화 억제 효과는 없었지만 강한 항산화 효과가 있었다. 이들 결과는 개발된 빵이 비만예방 및 억제뿐만 아니라 산화적 스트레스에 의해 유발되는 질병에 도움을 줄 수 있는 건강빵이라는 것을 제안하였다.