• 대한수의학회지
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• 제40권3호
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• pp.431-437
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• 2000

The aim of this study was to evaluate the involvement of CPP32 (caspase-3), one of the death-related enzymes, in the course of experimental autoimmune encephalomyelitis (EAE). EAE was induced in Lewis rats immunized with an emulsion of rat spinal cord homogenate with complete Freunds adjuvant supplemented with Mycobacterium tuberculosis (H37Ra, 5mg/ml). The expression of CPP32 in the spinal cords of rats with EAE was studied. In normal rat spinal cords, CPP32 is constitutively, but weakly, expressed in neurons and some neuroglial cells. In the EAE spinal cords, many inflammatory cells were positive for CPP 32, and the majority of CPP32(+) cells were identified as ED1(+) macrophages. During this stage of EAE, the number of CPP32(+) cells in brain cells, including neurons and astrocytes, increased, and these cells also had increased CPP32 immunoreactivity. CPP32 immunor eactivity was not always matched with apoptosis of inflammatory cells in EAE lesions. We speculate that CPP32, which is constitutlvely expressed in brain cells, increases in response to neuroimmunological stimulation in both brain neuronal cells and inflammatory cells. The functional role of CPP32 in neuroimmunological disorders is discussed.

• 대한한의학회지
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• 제21권1호
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• pp.53-61
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• 2000

Objectives: This study was camed out to examine the effect of five fractions of aqueous extract from Artemisia capillaris THUNB(ACT), on TGF, 1-induced apoptosis, cell viability, cell cycle progression and mRNA expression of apoptosis-related genes in human hepatocyte cell line HepG2. Methods: This study employed Tryphan blue exclusion assay, DNA fragmentation assay, Cpp32 protease activity assay and Quantitative RT-PCR analysis. Results: In the Tryphan blue exclusion assay, the butanol fraction of ACT with $TGF{\beta}$, l showed magnificent (Nice word, ut is it appropriate in a medical abstract\ulcorner) viability and the H2O fraction of ACT with $TGF{\beta}$, l also showed higher viability than only $TGF{\beta}$, l-treated group. DNA fragmentation assay showed that the butanol fraction and the H2O fraction carried inhibitory effects on apoptosis induction, with the butanol fraction displaying greater effects. The Cpp32 protease activity assay showed that the butanol fraction decreased Cpp32 protease activity. The H2O fraction of ACT had no significant effect on the Cpp32 protease activity. Quantitative RT-PCR showed that the butanol fraction suppressed Bax, p 15/INK4B, p21/Waf1, PAI-1 and increased Bcl-2 gene. Conclusions: The data shows that butanol fraction of ACT increases the hepatocyte viability and has the hepatocellular protective effect by the suppression of $TGF{\beta}$, l induced-apoptosis through gene regulation.

• 대한한의학회지
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• 제21권3호
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• pp.174-185
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• 2000

Objectives : This study was carried out to evaluate the effects of five fractions on cell viability, cell cycle progression and apoptosis. Methods : This study employed MTT assay, Cell cycle analysis, Cpp32 protease assay, DNA fragmentation assay and Quantitative RT-PCR analysis. Results : In MTT assay, the butanol fraction of Injinsaryung-San has showed magnificent viability, while the $H_2O$ fraction and ethylacetate fraction also showed higher viability than the control group. The $H_2O$ fraction of Injinsaryung-San has showed magnificent viability, and butanol fraction and ethylacetate fraction of Injinsaryung-San with etoposide have also showed higher viability than the only etoposide group. Cell cycle analysis showed that each fraction of Injinsaryung-San had no significant effect on the cell cycle. DNA fragmentation assay showed that the butanol fraction, $H_2O$ fraction and ethylacetate fraction carried inhibitory effects on apoptosis induction. Cpp32 protease activity assay showed that the butanol fraction, $H_2O$ fraction and ethylacetate fraction decreased Cpp32 protease activity, with the butanol fraction displaying greater effects. Quantitative RT-PCR showed that the butanol fraction, $H_2O$ fraction and ethylacetate fraction suppressed Fas and Bax genes, the butanol fraction increased BcI-2 gene, however no effect on Cpp32. Conclusions : The data shows that the butanol fraction of Injinsaryung-San increases the hepatocyte viability and has the heptocelluar protective effect by the suppression of apoptosis through gene regulation.

• 대한한의학회지
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• 제19권2호
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• pp.337-372
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• 1998

To evaluate the effects of Injinchunggantang-derivative on cell viability, cell cycle progression, and apoptosis, MTT assay, cell cycle analysis, Cpp32 protease assay, DNA fragnemtation assay, quantitative RT-PCR, and Western blotting were performed. The results were as followes. In MTT assay, etoposide+Injinchunggantang-derivative-treated cells as well as Injinchunggantang-derivative-treated cells showed higher viability than etoposide-treated cells with no time-concentration-dependence, which implied that Injinchunggantang-derivative has hepato-protective effect Cell cycle analysis showed that Injinchunggantang-derivative has no significant effect on the cell cycle. Cpp32 protease assav and DNA fragmentation assay Injinchunggantang-derivative carry inhibitory effects on apoptosis induction. It was suggested that Injinchunggantang-delivative might regulate the cell cycle, in particular $G_1$ checkpoint by blocking p53 and Watl pathway. Injinchunggantang-derivative inhibited the mRNA expressions of Cpp32, Fas, and Bcl-2, which could result in inhibition of apoptosis. These results imply that Injinchunggantang-derivative increases hepatocyte viability, and protects hepatocyte from damage by regulating the expression of genes associated with cell cycle and apoptosis, which explains the mechanism of the clinical effect of Injinchunggantang-derivative on liver diseases.

• 생물정신의학
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• 제4권2호
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• pp.211-217
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• 1997

The Bcl-2 protein has been shown to block apoptosis induced by a variety of stimuli. We have performed the experiments which cell death can be blocked by the bcl-2 proto-oncogene under moderate(50-100mM) or high ethanol treatment(400-600mM). As a result of morphological changes, and MTT assay, cell death was blocked by Bcl-2 under 100mM ethanol. However, the results of DNA fragmentation and RT-PCR(ICE, and CPP32), immunoblotting(CPP32, and PARP) for SK-pcDNA3 cells(vector only) and SK-Bcl-2 cells(stably expressed bcl-2 gene) were showen to be no significant differences between two cell lines. These results suggested that cell death induced by ethanol was not followed by apoptosis mechanism, and was blocked by the bcl-2 proto-oncogene with moderate ethanol.

• 한국세라믹학회지
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• 제32권11호
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• pp.1301-1307
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• 1995

Pitting corrosion of TiN film deposited on Inconel 600 by plasma assisted chemical vapor deposition (PACVD) was investigated. Cyclic potentiodynamic polarization (CPP) tests were conducted in order to determine the pit nucleation potentials, Enp, of the TiN-deposited sample and the bare Inconel 600 in deaerated NaCl solution at 25, 135 and 20$0^{\circ}C$. The effects of the TiN film thickness, the solution temperature and the Cl- concentration on Enp were studied. Enp of the TiN-deposited sample which had the film thickness above 1${\mu}{\textrm}{m}$ were higher than those of the bare Inconel 600 by 300~600mV at all the solution temperatures, implying the pitting resistance improvement of the TiN film. The morphologies of the pits generated after immersion test were examined with a scaning electron microscopy. The higher was the solution temperature, the more corrosion products, mainly composed of Cr and Ni oxides, were formed.

• 한국지능시스템학회논문지
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• 제19권2호
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• pp.206-211
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• 2009

본 논문은 가중 퍼지소속함수 기반 신경망(Neural Network with Weighted Fuzzy Membership Functions, NEWFM)기반의 자동 특징 추출기법을 사용하여 1일 후의 주식 예측을 하는 방안을 제안하고 있다. 비중복면적 분산측정 법에 의해 중요도가 가장 낮은 특징입력을 자동적으로 하나씩 제거하면서 최소의 특징입력을 선택하였다. 특징입력으로써 CPP$_{n,m}$(Current Price Position of the day n)과 최근 32일간의 CPP$_{n,m}$을 웨이블릿 변환한 38개의 계수들 중 비중복면적 분산측정법에 의해서 자동적으로 추출된 2개의 계수가 사용되었다 제안된 방법으로 1989년부터 1998년까지의 실험군을 사용한 결과로써 60.93%의 예측율을 나타내었다.

• 한국산학기술학회논문지
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• 제18권11호
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• pp.399-408
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• 2017

본 연구는 진로지도준비교육이 대학생의 셀프리더십과 진로내외통제위에 미치는 영향을 확인하기 위하여 수행되었다. 목표를 성취하기 위하여 스스로를 통제하고 이끌어 가는 능력인 셀프리더십과 진로 및 취업결과에 대해 가족 및 환경 등 외부의 탓으로 여기거나 스스로 역량이나 준비 등 자신의 능력 등 내부의 탓으로 생각하는 진로내외통제위는 취업에 대한 중요한 변수로 알려져 있다. 연구에 대한 목적과 참여방법을 설명하고 이에 동의한 대학생 58명을 대상으로 15주간 진로준비 프로그램을 제공하였고, 자료수집은 2016년 3월부터 6월까지 D시에 위치한 H대학교에서 수행되었다. 진로준비프로그램은 취업 준비에 대한 구체적인 내용뿐만 아니라 구직을 준비하는 학생 스스로 자신에 대한 이해를 높이도록 자기탐색 및 자기주도 기반으로 구성되었다. 프로그램의 사전과 사후 시점에서 셀프리더십과 진로자기통제위가 측정되었고 SPSS 21.0으로 분석되었다. 연구결과, 자기탐색과 자기주도기반의 진로준비프로그램은 학생들의 셀프리더십을 높였고(t=-4.45, p=. 00), 진로내외통제위 중 특히 내적통제위를 높이는 것으로 확인되었다(t=-3.26, p=. 00). 따라서 본 연구결과 추후 자기탐색을 기반으로 하는 진로지도 프로그램을 대학생의 진로준비에 적극적으로 적용 할 것을 제안한다.

• 말소리와 음성과학
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• 제6권3호
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• pp.63-72
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• 2014

The current study assessed the utility of acoustic analyses the most commonly used in routine clinical voice assessment including perturbation, nonlinear dynamic analysis, and Spectral/Cepstrum analysis based on signal typing of dysphonic voices and investigated their applicability of clinical acoustic analysis methods. A total of 70 dysphonic voice samples were classified with signal typing using narrowband spectrogram. Traditional parameters of %jitter, %shimmer, and signal-to-noise ratio were calculated for the signals using TF32 and correlation dimension(D2) of nonlinear dynamic parameter and spectral/cepstral measures including mean CPP, CPP_sd, CPPf0, CPPf0_sd, L/H ratio, and L/H ratio_sd were also calculated with ADSV(Analysis of Dysphonia in Speech and VoiceTM). Auditory perceptual analysis was performed by two blinded speech-language pathologists with GRBAS. The results showed that nearly periodic Type 1 signals were all functional dysphonia and Type 4 signals were comprised of neurogenic and organic voice disorders. Only Type 1 voice signals were reliable for perturbation analysis in this study. Significant signal typing-related differences were found in all acoustic and auditory-perceptual measures. SNR, CPP, L/H ratio values for Type 4 were significantly lower than those of other voice signals and significant higher %jitter, %shimmer were observed in Type 4 voice signals(p<.001). Additionally, with increase of signal type, D2 values significantly increased and more complex and nonlinear patterns were represented. Nevertheless, voice signals with highly noise component associated with breathiness were not able to obtain D2. In particular, CPP, was highly sensitive with voice quality 'G', 'R', 'B' than any other acoustic measures. Thus, Spectral and cepstral analyses may be applied for more severe dysphonic voices such as Type 4 signals and CPP can be more accurate and predictive acoustic marker in measuring voice quality and severity in dysphonia.

• 대한한방부인과학회지
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• 제32권4호
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• pp.102-115
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• 2019

Objectives: This study aimed to review randomized controlled trials (RCTs) on the effects of interventions of Korean medicine for chronic pelvic pain (CPP). Methods: We searched eleven electronic databases from inception up to Apr 2018. RCTs evaluating the effects of Korean medicine interventions for CPP were retrieved. Results: 11 RCTs were included and total number of experimental group was 390 cases. A total of 4 types of interventions were used, of which acupuncture (45.5%), electro-acupuncture (18.2%), electro-acupuncture + auricular acupuncture + moxibustion (18.2%) were the most frequently utilized. 關元 (CV4) (100.0%), 會陰 (CV1) (80.0%), 三陰交 (SP6) (80.0%), 陰陵泉 (SP9) (80.0%) were most frequently used acupoint in acupuncture treatment. Conclusions: For evidence-based treatment of korean medicine intervention for CPP, high quality RCTs must be conducted.