Korean Journal of Biological Psychiatry (생물정신의학)
- Volume 4 Issue 2
- /
- Pages.211-217
- /
- 1997
- /
- 1225-8709(pISSN)
- /
- 2005-7571(eISSN)
Cell Death Induced by Ethanol : Prevention of Cell Death by the bcl-2 Proto-Oncogene
에탄올 유래 세포사망 : bcl-2 proto-oncogene에 의한 세포사망 저해
- Lim, Eun-Jeong (Department of Biochemistry and Molecular Biology, College of Science, Hanyang University) ;
- Hong, Kyoung-Ja (Department of Biochemistry and Molecular Biology, College of Science, Hanyang University) ;
-
Yang, Byung-Hwan
(Department of Neuropsychiatry, College of Medicine & The Mental Health Research Institute, Hanyang University)
;
-
Chai, Young-Gyu
(Department of Biochemistry and Molecular Biology, College of Science, Hanyang University)
- 임은정 (한양대학교 이과대학 생화학 및 분자생물학과) ;
- 홍경자 (한양대학교 이과대학 생화학 및 분자생물학과) ;
-
양병환
(한양대학교 의과대학 신경정신과학교실 및 정신건강연구소)
;
-
채영규
(한양대학교 이과대학 생화학 및 분자생물학과)
- Published : 1997.12.25
Abstract
The Bcl-2 protein has been shown to block apoptosis induced by a variety of stimuli. We have performed the experiments which cell death can be blocked by the bcl-2 proto-oncogene under moderate(50-100mM) or high ethanol treatment(400-600mM). As a result of morphological changes, and MTT assay, cell death was blocked by Bcl-2 under 100mM ethanol. However, the results of DNA fragmentation and RT-PCR(ICE, and CPP32), immunoblotting(CPP32, and PARP) for SK-pcDNA3 cells(vector only) and SK-Bcl-2 cells(stably expressed bcl-2 gene) were showen to be no significant differences between two cell lines. These results suggested that cell death induced by ethanol was not followed by apoptosis mechanism, and was blocked by the bcl-2 proto-oncogene with moderate ethanol.