• The Korean Journal of Malacology
• /
• v.29 no.3
• /
• pp.251-258
• /
• 2013

This study aims to find out a suitable cryoprotective agent (CPA) for cryopreservation and its optimum concentration in order to conduct planned artificial seed production of Pacific oyster, Crassostrea gigas and to preserve superior sperm. For this, we tried to understand toxicity and the effect of cryopreservation by CPA type and concentrations first and then looked into cell damage of the sperm after thawing. Toxicity analysis on the sperm of Pacific oyster according to different CPA and immersion time shows that dimethyl sulfoxide (DMSO) comes first when it comes to survival rate and mobility followed by ethylene glycol (EG), glycerol and methanol. To identify the optimum CPA and its level, filtered seawater was used as a diluent before cryopreservation for 30 days. As a result, cryopreserved sperm of Pacific oyster with 15% of DMSO showed the highest survival rate and activation. Also, we observed the cryopreserved and thawed sperm with Scanning electron micrographs by CPAs and concentrations. Consequently, DSMO showed the lowest cell damage followed by EG, methanol, glycerol and the level was 15, 20, 10, 5% respectively. In a nutshell, it is proven that the optimum CPA and its level is 15% of DMSO.

• Journal of the Mineralogical Society of Korea
• /
• v.15 no.3
• /
• pp.161-169
• /
• 2002

high pressure and temperature conditions. However, those results are not consistent with one another, and phase boundary between ilmenite and perovskite phases determined only from the quenching method may be not so reliable at all. Therefore, in-situ high pressure-temperature (hP-T) X-ray diffraction measurements were performed up to 19 GPa and $700^{\circ}C$ in a large volume press apparatus using synchrotron radiation. Experimental results show that perovskite phase is stable at pressures above 16 GPa, and transforms back to $LiNbO_3$phase near 15 CPa at room temperature, and that the perovskite-ilmenite transition is back and forth near 15 CPa at $500^{\circ}C$. LiNbO$_3$phase transforms to ilmenite at 13 CPa and $300^{\circ}C$ and at 10.8 CPa and $400^{\circ}C$, respectively. These data indicate that $LiNbO_3$phase may have a stability region in the hP-T phase diagram and that the perovskite-ilmenite phase boundary would be quite different from that previously reported.

• Molecules and Cells
• /
• v.43 no.11
• /
• pp.945-952
• /
• 2020

Hypoxia induces the expression of several genes through the activation of a master transcription factor, hypoxia-inducible factor (HIF)-1α. This study shows that hypoxia strongly induced the expression of two carboxypeptidases (CP), CPA4 and CPE, in an HIF-1α-dependent manner. The hypoxic induction of CPA4 and CPE gene was accompanied by the recruitment of HIF-1α and upregulation in the active histone modification, H3K4me3, at their promoter regions. The hypoxic responsiveness of CPA4 and CPE genes was observed in human adipocytes, human adipose-derived stem cells, and human primary fibroblasts but not mouse primary adipocyte progenitor cells. CPA4 and CPE have been identified as secreted exopeptidases that degrade and process other secreted proteins and matrix proteins. This finding suggests that hypoxia changes the microenvironment of the obese hypoxic adipose tissue by inducing the expression of not only adipokines but also peptidases such as CPA4 and CPE.

• Journal of the Korea Institute of Information Security & Cryptology
• /
• v.30 no.1
• /
• pp.39-49
• /
• 2020

In this paper, we show that the Chinese standard block cipher SM4 is vulnerable to the side channel attacks and present a countermeasure to resist them. We firstly validate that the secret key of SM4 can be recovered by differential power analysis(DPA) and correlation power analysis(CPA) attacks. Therefore we analyze the vulnerable element caused by power attack and propose a first order masking-based countermeasure to defeat DPA and CPA attacks. Although the proposed countermeasure unfortunately is still vulnerable to the profiling power attacks such as deep learning-based multi layer perceptron(MLP), it can sufficiently overcome the non-profiling attacks such as DPA and CPA.

• Bulletin of the Korean Chemical Society
• /
• v.26 no.6
• /
• pp.935-938
• /
• 2005

Nucleophilic addition reactions of benzylamines (BA; $XC_6H_4CH_2NH_2$) to $\alpha-cyano-\beta$-phenylacrylamides (CPA; $YC_6H_4CH=C(CN)CONH_2$) have been investigated in acetonitrile at 25.0 ${^{\circ}C}$. The rate is first order with respect to BA and CPA and no base catalysis is observed. The addition of BA to CPA occurs in a single step in which the addition of BA to $C_{\beta}$ of CPA and proton transfer from BA to $C_{\alpha}$ of CPA take place concurrently with a four-membered cyclic transition state structure. The magnitude of the Hammett ($\rho_X$) and Bronsted ($\beta_X$) coefficients are rather small suggesting an early tansition state (TS). The sign and magnitude of the crossinteraction constant, $\rho_XY$ (= −D0.26), is comparable to those found in the normal bond formation processes in the $S_N2$ and addition reactions. The normal kinetic isotope effect ($k_H/k_D\;{\gt}$ 1.0) and relatively low ${\Delta}H^{\neq}$ and large negative ${\Delta}S^{\neq}$ values are also consistent with the mechanism proposed.

• Proceedings of the KIEE Conference
• /
• 2002.07b
• /
• pp.571-576
• /
• 2002

Electromagnetic analysis has been made for the design of 3 MJ Compulsator (Compensated Pulsed Alternator, CPA) that will be used as the power supply system of the electrothermal-chemical launcher system. using finite element method with ANSYS/Emag. From the analysis results, it was possible to decide the excitation MMF and the rotor/stator size to obtain the desired output current. And using electromagnetic forces from the analysis results. the stresses on the components of 3 MJ CPA could be analyzed. The detailed design based on the analysis results has been made and the fabrication of 3 MJ CPA is in progress. Before assembling components into 3 MJ CPA. an experiment is being made to check the correspondence between analysis results and measurements.

• 한국경영정보학회:학술대회논문집
• /
• 2008.06a
• /
• pp.428-433
• /
• 2008

The online advertising industry's business model undertakes the change from CPM (cost-per-mille)-based to CPC (cost-per-click)-based. However, due to the problem of 'Click Fraud', CPA (cost-per-action) has been regarded as a new step. For CPA, publishers need to get information after a user clicks an advertisement. Therefore, in CPA, the key is to get Conversion Action Data (CAD). This paper introduces two existing mechanisms for getting CAD, compare their characteristics, and analyze their limitations. Then the two new mechanisms are introduced and their requirements and feasibility are analyzed. Lastly, we compare the existing two and the new two mechanisms, and point out each mechanism's business possibility, value and Application Area. This paper will help publishers choose the most appropriate mechanism on the basis of their situation.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
• /
• 2012.10a
• /
• pp.157-159
• /
• 2012

해상 교통량 증가에 따른 연안해역 해상교통밀집도가 증가되고 있다. 그러므로 해상교통안전관리를 위해 실 해역의 해상교통패턴을 파악하여 해상교통지수를 평가하는 것이 중요하다. 분석대상해역의 실제 선박위치, 속력, 침로를 고려한 CPA-TCPA분석은 통계적인 선박데이터에 의한 분석보다 해당해역의 실제적인 VTS영향 등 교통환경을 반영한다. 본 연구에서는 횡간수도와 완도진입수로의 항로가동률을 분석하고, 해당 해역에서 교차되는 선박들의 CPA, TCPA 등을 분석하여 해상교통안전지수를 평가하고자 한다. 또한 산출된 해상교통안전지수를 해당 해역의 통계적인 해상교통안전지수와 비교 및 분석하고자 한다.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
• /
• 2020.11a
• /
• pp.39-41
• /
• 2020

충돌 회피를 위한 위험도 평가에서 CPA 계산은 핵심 요소이며, AIS의 도입 이후 AIS 정보를 이용한 CPA 계산이 필수적으로 활용되고 있다. 본 논문에서는 CPA 계산에 이용되는 AIS 정보와 실제 특성을 확인하고, 선박 위치 추정에 관련된 선행 연구들을 분석하며, 이를 가상 디지털 항로 표지 서비스에 적용할 방안을 강구한다, P. Last 등의 연구는 ROT의 중요성을 강조하고 있으나, 대부분의 선박이 필요로 하는 정확한 ROT 값을 전송하지 못하고 있는 상황을 확인했고, Ling-Zhi Sang 등의 연구는 4가지 요소를 반영한 간단한 식으로 구현하여 전 해역의 데이터로 비교한 결과 2가지 요소만을 적용한 방법에 대한 정확도의 향상을 확인할 수 없었다.

• Proceedings of the Optical Society of Korea Conference
• /
• 2006.02a
• /
• pp.141-142
• /
• 2006