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Expression of Estrogen and Progesterone Receptors in Non-small-cell Lung Cancer Tissue Using Tissue Microarray Method (조직 미세배열법을 이용한 비소세포 폐암 조직에서 에스트로겐과 프로게스테론 수용체 발현)

  • Han, Hye-Seung;Kim, Min-Ji;Cho, Jae-Hwa;Yoon, Yong-Han;Kwak, Seung-Min;Lee, Hong-Lyeol;Kim, Kwang-Ho;Ryu, Jeong-Seon
    • Tuberculosis and Respiratory Diseases
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    • v.58 no.1
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    • pp.54-58
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    • 2005
  • Background : To evaluate the role of estrogen and progesterone in the carcinogenesis of NSCLC, IHC studies for the expression of the receptors of estrogen and progesterone have been performed with inconsistent results. Recently the TMA method has been developed and has become recognized as a useful and rapid method for extensively analysing molecular markers at the gene and protein level. We have investigated their expressions in the tissue from NSCLC using the microarray method. Methods : The TMA construction was made with 70 formalinfixed, paraffin-embedded tissues of NSCLC. After heat-induced epitope retrieval, IHC staining on primary tissues of NSCLC was performed with the monoclonal antibodies, ER1D5 and PR1A6. Results : Our sample of 70 consisted of 74% men and 26% women. Of the patients, 49% were current smokers, 27% were non-smokers and 24% were former smokers. By histologic classification, 34 patients had squamous cell carcinoma, 24 had adenocarcinoma, 9 had adenosquamous cell carcinoma, and 3 had other carcinomas. No cancer cells were immunostained with these monoclonal antibodies in any primary tissues of NSCLC. Conclusions : No expression of neither of the two receptors was found in any of the lung cancer tissues. This suggests that adequate genetic variants for IHC staining need to be developed for NSCLC.

The Development and Acceptance of Knowledge Information in Garden of Joseon Dynasty - Focusing on the Garden and Flowering Books Compiled from the 15th and 19th Centuries - (조선시대 정원의 지식정보 전개와 수용 - 15~19세기 편찬된 정원 및 화훼 관련서적을 중심으로 -)

  • Kim, Dong-Hyun;Lee, Won-Ho
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.38 no.1
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    • pp.10-20
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    • 2020
  • This study aims to analyze the developed characteristics of the knowledge and information of gardens through garden or flowering plant books compiled in the 15th and 19th centuries of Joseon Dynasty. Diachronically analysis of the garden or flowering plant books classified the characteristics in which knowledge and information about gardens are developed by the period, and looked at the factors. The results are as follows; First, the relationship between the authors who compiled the garden or flowering plant books had similar characteristics to the genealogy of Realist School of Confucianism(實學) in the Joseon Dynasty. Kang, Hee-An's practical features influenced later realist school of confucianism scholars. Lee, Su-Gwang has accumulated knowledge of the garden through his experience of traveling the diplomatic envoy to China. Since then, Hong Man-sun's ideology has been related to Charles, a member of the Southerners. Seo Yu-gu was also able to accept Realist School of Confucianism in an integrated way through the Jungnong school's theory and interaction with the Jungsang school. Ryu, Jung-Lim's relationship with the Jungnong school emerged as he added to the 『Jeungbosanrimgyeongje(增補山林經濟)』. Second, the 『Yanghwasorok(養花小錄)』, 『Jibongyuseol(芝峯類說)』 「Hwuimok(卉木)」, 『Hangjeongrok(閑情錄)』, 『Sanrimgyeongje(山林經濟)』 「Yanghwa(養花)」, 『Jeungbosanrimgyeongje(增補山林經濟)』 「Yanghwa(養花)」, 『Hwaamsurok(花庵隨錄)』 and 『Imwongyeongjeji(林園經濟志)』 「Yewonji(藝畹志)」 contain garden plant characteristics, cultivation methods, and management methods. The 『Imwongyeongjeji(林園經濟志)』 「Seomyongji(贍用志)」, 「Iunji(怡雲志)」, 「Sangtaekji(相宅志)」 contain details on the location selection of gardens, the layout of facilities, how to create them and materials. The description of these garden or flowering plant books was found to be the most common introduction with 55 percent, followed by methodologies(42.8%), the Lichi Theory(理氣論, 15.5%), the classification(12.4%), and the convention(1.9%). Third, based on the importance of knowledge and information on gardens, the garden or flowering plant books related to the period were classified as early period, including 『Yanghwasorok(養花小錄)』, 『Jibongyuseol(芝峯類說)』 which were compiled before the 17th century. The 18th-century compiled 『Sanrimgyeongje(山林經濟)』 and 『Jeungbosanrimgyeongje(增補山林經濟)』 were classified as middle period, and the 19th-century compilation of 『Imwongyeongjeji(林園經濟志)』 was classified as late period. The garden or flowering plant books were cited the contents of ancient Chinese books, the author's experiences and opinions contained in the preceding period in later garden books. And the reinforcement of garden knowledge was made to reflect the agricultural technology and expertise developed at the time of writing. Fourth, based on analysis of the development and acceptance of knowledge information in garden by period, In the early period was dealing with floriculture as a way to explore the logic of things. Later, in the 18th century, a vast influx of garden knowledge information came from China. Among scholars, they secured justification for garden creation as part of various knowledge-seeking activities, which expanded their expertise in gardens. In response to the trend of gardening in the 19th century, professional books were written based on knowledge and information on gardens that were collected in the past, and systems were established such as the collection and management of garden plants, construction methods, enjoying methods, and self-realization.

Evaluation of Filtration and Backwash Efficiency of Non-point Source Pollution Reduction Facility (장치형 비점오염원 저감시설의 여과 및 역세 효율 평가)

  • Yun, Sangleen;Lee, Yong-Jae;Ahn, Jae-Hwan;Choi, Won-Suk;Lee, Jungwoo;Oh, Hye-Cheol;Kim, Seog-Ku
    • Journal of Korean Society of Environmental Engineers
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    • v.39 no.12
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    • pp.664-671
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    • 2017
  • Non-point source pollution is the emission source that unspecifically releases pollutants to water system from unspecific places such as cities, agricultural lands, mountains, and construction sites and its discharge path is not easily identified. Also, it is difficult to design and manage the reduction facilities for the emission quantity is primarily affected from weather conditions like rainfall. Since 2006, the significance of non-point source pollution reduction has been grown in Republic of Korea and this reinforces needs for the installation of reduction facilities. However, because the standards for the installation details and reduction efficiency are not clarified by law, people are preferring technologies that do not require particular maintenance and high expenses. The purpose of this study is to examine and maintain the efficiency of non-point source pollutants reduction facility which uses expended polypropylene as a media. The higher the depth of the media, the less range of variations in the reduction efficiency was observed and the final efficiency was also increased. When the media depth was 60 cm, the average reduction efficiency was 94% and 90% where linear velocities were 10 m/hr and 20 m/hr respectively. The results from 180 minutes operation in 10 m/hr and 20 m/hr of linear velocities were slightly different in head loss changes which were caused by media depth variations. The backwash experiments which were conducted in triplicate showed the reduction efficiency decreased as the time went on because of the media clogging. However, it was found that after the backwashing the reduction efficiency was increased as effective as the efficiency of the initial filtration.

Emergy Evaluation of the Estuarine Areas of Yeongsan River, Seomjin River, and Han River in Korea (영산강, 섬진강, 한강 하구역의 에머지 평가)

  • Lee, Chang-Hee;Kang, Dae-Seok
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.14 no.2
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    • pp.135-143
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    • 2008
  • An emergy concept was used to evaluate the environment and economy of the estuarine areas of Yeongsan River, Seomjin River, and Han River in Korea. The emergy evaluations clearly showed ecological and socioeconomic characteristics of the estuarine areas that act as ecological and economic centers of surrounding areas. River, tide, and rain provided most of the renewable emergy inputs to the estuarine areas with their contribution to the total emergy input less than 8%. The estuarine areas mostly relied for their operation on the purchased emergy which accounted for $92{\sim}98%$ of the total emergy input to the systems. Emergy indices such as emergy use per unit area, population carrying capacity, environmental loading ratio, and emergy sustainability index revealed that the estuarine areas of Yeongsan River, Seomjin River, and Han River are not sustainable at the current level of economic activities in the areas. The ecological economic values of the environment of the areas were in the range of $7.29{\sim}22.06$ million Em\/ha/yr. They are more than twice that for the whole country, indicating the ecological and economic importance of the eatuarine areas. It is, therefore, urgent to establish and implement estuarine management policies to protect and restore the ecological and economic potentials of the estuarine areas of Yeongsan River, Seomjin River, and Han River. Management plans for the estuarine areas should include both demand-side measures such as reduction of population and economic concentration and consideration of ecological carrying capacity in planning stages for utilization and development of the areas, and supply-side ones such as restoration of degraded ecosystems and construction of new productive ecosystems.

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Simple Formulae for Buckling and Ultimate Strength Estimation of Plates Subjected to Water Pressure and Uniaxial Compression (수압(水壓)과 압축력(壓縮力)을 받는 평판(平板)의 좌굴(挫屈) 및 최종강도(最終强度) 추정식(推定式))

  • Jeom-K.,Paik;Chang-Y.,Kim
    • Bulletin of the Society of Naval Architects of Korea
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    • v.25 no.4
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    • pp.69-80
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    • 1988
  • This paper proposes simple formulae for buckling and ultimate strength estimation of plates subjected to water pressure and uniaxial compression. For the construction of a formula for elastic buckling strength estimation, parametric study for actual ship plates with varying aspect ratios and the magnitude of water pressure is carried out by means of principle of minimum potential energy. Based on the results by parametric study, a new formula is approximately expressed as a continuous function of loads and aspect ratio. On the other hand, in order to get a formula for ultimate strength estimation, in-plane stress distribution of plates is investigated through large deflection analysis and total in-plane stresses are expressed as an explicit form. By applying Mises's plasticity condition, ultimate strength criterion is then derives. In the case of plates under relatively small water pressure, the results by the proposed formulae are in good agreement compared with those by other methods and experiment. But present formula overestimates the ultimate strength in the range of large water pressure. However, actual ship plates are subjected to relatively small water pressure except for the impact load due to slamming etc.. Therefore, it is considered that present formulae can be applied for the practical use.

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Planting Method of Buffer Green Space in the Reclaimed Seaside Areas, Rokko Island, Kobe, Japan (일본 고베시(신호시(神戶市)) 로코(육갑(六甲))아일랜드 임해매립지의 완충녹지 식재기법 연구)

  • Han, Bong-Ho;Kim, Jong-Yup;Choi, Jin-Woo;Cho, Yong-Hyeon
    • Korean Journal of Environment and Ecology
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    • v.24 no.2
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    • pp.157-165
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    • 2010
  • This study was carried out to suggest the basic data of planting method for construction of buffer green space based on the land use in case of reclaimed land by analyzing land structure, planting concept, and planting structure in buffer green space, Rokko Island, Kobe, Japan. Rokko Island(total area: 580ha) is divided into port and logistics industry area and urban area by constructing the box type large-scale buffer green space. The land structure of buffer green space were biased mounding type, parallel mounding type, and complex mounding type. The width of buffer green space was 50meters in case of northern area, from 28 to 32meters in case of eastern area, and 37.5meters in case of western area, and the slope of that was from 18 to 25 degrees and the height of that was from 2 to 15meters. There were applied landscape and buffer planting concept on the sea side area of northern buffer green space, on the other hand landscape and shade planting concept on the Inner city side area of that. According to the result of planting structure analysis of northern buffer green space, the main woody species were those of deciduous-evergreen species grow in warm-temperate forest zone such as Quercus glauca, Cinnamomum camphora, Machilus thunbergii, Elaeagnus maritima. The results of maximum number of species and planting density by $100mm^2$ was that 9 species 22 individuals in canopy layer, 9 species 15 individuals in understory layer, 3 species 67 individuals in shrub layer, and 14 species 104 individuals in total. The plant coverage of northern buffer green space based on the ecological planting method was from 69 to 139% in case of canopy layer, from 26 to 38% in case of understory layer, from 6 to 7% in case of shrub layer, and from 101 to 184% in total. Index of plant crown volume of northern buffer green space based on the ecological planting method was from 1.40 to $3.12m^3/m^2$ in case of canopy layer, from 0.43 to $0.55m^3/m^2$ in case of understory layer, $0.06m^3/m^2$ in case of shrub layer, and from 1.89 to $3.73m^3/m^2$ in total.

Avifauna and Management of Breeding Season in Taeanhaean National Park (태안해안국립공원의 번식기 조류상과 관리)

  • Paik, In-Hwan;Jin, Seon-Deok;Yu, Jae-Pyoung;Paek, Woon-Kee
    • Korean Journal of Environment and Ecology
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    • v.24 no.2
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    • pp.139-146
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    • 2010
  • The survey was done in order to find what kinds of birds visit Taeanhaean National Park during breeding season, where we fixed up 10 coastal areas and islands within the National Park. Three groups concurrently performed the field research from 5th to 9th of July in 2009. Total 58 species and 7,323 individuals were recorded in Taeanhaean National Park. 48 species including 6,187 individuals were observed in coastal areas and 33 species including 1,136 individuals in island areas. The most dominant species in the National Park are Larus crassirostris which accounts for 60% of the birds inhabiting there, and they seem to have been bred in the islands near the National Park. The birds observed only around the coastal areas include Anas poecilorhyncha, Fulica atra, Egretta intermedia and the others which consist of 25 species and amount to 318 individuals, and the birds found exclusively in island areas include Phalacrocorax filamentosus, Apus pacificus¸ Locustella pleskei and other birds, which consist of 10 species and the number of those individuals observed was 308. The inhabited islands areas such as Gauido were characterized by high ratio of waterbird population, which seems to be correlated with the factors such as the extent of island, the richness of water resources, and the diversity of habitats. Based on the data collected during the research and other data from the previous observations, the kinds of dominant species remain nearly unchanged. And in spite of the oil spill accident in 2007, the increase in the number of waterbirds compared to 2004 may be the evidence that the area is recovering from the environmental pollution. At present, the tidal power plants are being built or scheduled to be built and large-scale reclamation is also under way. What is worse, those areas are seeing the increase of pension construction, which is likely to be the potential cause of damage and disturbance against some key habitats for the waterbirds. Therefore, it is a major priority that we build the bird information system to efficiently manage the knowledge-based asset collected from bird-watching groups and to better monitor the areas that need enhanced database through which the National Park can be appropriately administered.

A Comparative Study of Juvenile Black-faced Spoonbills Platalea Minor Home Range in Gujido and Chilsando Islets, South Korea (구지도, 칠산도 저어새 유조의 행동권 비교 연구)

  • Son, Seok-Jun;Kang, Jung-Hoon;Kwon, In-Ki;Kim, Dal-Ho;Lee, Ki-Sup;Yoo, Jeong-Chil
    • Korean Journal of Environment and Ecology
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    • v.34 no.2
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    • pp.99-105
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    • 2020
  • Migratory birds use a variety of breeding and wintering sites, and it is particularly important to understand more information on breeding and feeding sites for the conservation and management of endangered species. Black-faced spoonbills (Platalea minor) are an international endangered species distributed in East Asia. The majority of black-faced spoonbills breed on uninhabited islets off the west coast of the Korean Peninsula during the breeding season, and they are distributed in East Asia such as Taiwan, Hong Kong, southern China, Japan, and Jeju island during the winter season. In this study, we used a wild animal location tracking system to analyze and compare home ranges of three black-faced spoonbills spending the post-fledging stage in Gujido islet in Incheon and Chilsando islet in Yeonggwang each in 2015. The tree black-faced spoonbills in Guji islet showed a home range in coastal areas in Hwanghaenam-do and Gangneung-gun. The home range size (mean±SD) was estimated to be 425.49±116.95 ㎢ using 100% MCP, 43.61±18.51 ㎢ using KDE 95%, and 7.46±3.68 ㎢using KDE 50%. The tree black-faced spoonbills in Chilsando islet showed a home range in the Baeksu tidal flat and the Buan Saemangeum area with a size of 99.38±55.29 ㎢ using 100% MCP, 19.87±6.05 ㎢ using KDE 95%, and 1.16±0.53 ㎢ using KDE 50%. The figured indicated that the tree black-faced spoonbills breeding in Gujido islet had a wider home range than those breeding in Chilsando islet. During the post-fledging stage, the home ranges of black-faced spoonbills were mostly breeding in mudflats. Therefore, it is necessary to minimize human intervention, such as the construction of roads and structures and the human access, to protect the habitats during the period.

A Study on the Effects of BIM Adoption and Methods of Implementationin Landscape Architecture through an Analysis of Overseas Cases (해외사례 분석을 통한 조경분야에서의 BIM 도입효과 및 실행방법에 관한 연구)

  • Kim, Bok-Young;Son, Yong-Hoon
    • Journal of the Korean Institute of Landscape Architecture
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    • v.45 no.1
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    • pp.52-62
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    • 2017
  • Overseas landscape practices have already benefited from the awareness of BIM while landscape-related organizations are encouraging its use and the number of landscape projects using BIM is increasing. However, since BIM has not yet been introduced in the domestic field, this study investigated and analyzed overseas landscape projects and discussed the positive effects and implementation of BIM. For this purpose, landscape projects were selected to show three effects of BIM: improvement of design work efficiency, building of a platform for cooperation, and performance of topography design. These three projects were analyzed across four aspects of implementation methods: landscape information, 3D modeling, interoperability, and visualization uses of BIM. First, in terms of landscape information, a variety of building information was constructed in the form of 3D libraries or 2D CAD format from detailed landscape elements to infrastructure. Second, for 3D modeling, a landscape space including simple terrain and trees was modeled with Revit while elaborate and complex terrain was modeled with Maya, a professional 3D modeling tool. One integrated model was produced by periodically exchanging, reviewing, and finally combining each model from interdisciplinary fields. Third, interoperability of data from different fields was achieved through the unification of file formats, conversion of differing formats, or compliance with information standards. Lastly, visualized 3D models helped coordination among project partners, approval of design, and promotion through public media. Reviewing of the case studies shows that BIM functions as a process to improve work efficiency and interdisciplinary collaboration, rather than simply as a design tool. It has also verified that landscape architects could play an important role in integrated projects using BIM. Just as the introduction of BIM into the architecture, engineering and construction industries saw great benefits and opportunities, BIM should also be introduced to landscape architecture.

Scientific Analysis of the Historical Characteristics and Painting Pigments of Gwaebultaeng in Boeun Beopjusa Temple (보은 법주사 <괘불탱>의 미술사적 특징과 채색 안료의 과학적 분석 연구)

  • Lee, Jang-jon;Gyeong, Yu-jin;Lee, Jong-su;Seo, Min-seok
    • Korean Journal of Heritage: History & Science
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    • v.52 no.4
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    • pp.226-245
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    • 2019
  • Beopjusa Gwaebultaeng (Large Buddhist Painting), designated as Treasure No. 1259, was painted in 1766 and featured Yeorae (Buddha) at the center in the style of a single figure. It is the longest existing buddhist painting and was created by Duhun, a painter who was representative of 18th century Korean artists. His other remaining work is Seokgayeorae Gwaebultaeng (1767) in Tongdosa Temple. Considering their same iconography, they are assumed to have used the same underdrawing. Duhun had a superb ability to maintain a consistent underdrawing, while most painters changed theirs within a year. The Beopjusa painting carries significance because it was not only painted earlier than the one in Tongdosa, but also indicates possible relevance to the royal family through its records. Beopjusa Temple is also the site of Seonhuigung Wondang, a shrine housing the spirit tablet of Lady Yi Youngbin, also known as Lady Seonhui. Having been built only a year before Beopjusa Gwaebultaeng was painted, it served as a basis for the presumption that it has a connection to the royal family. In particular, a group of unmarried women is noticeable in the record of Beopjusa painting. The names of some people, including Ms. Lee, born in the year of Gyengjin, are recorded on the Bonginsa Temple Building, the construction of which Lady Yi Youngbin and Princess Hwawan donated money to. In this regard, they are probably court ladies related to Lady Yi Youngbin. The connection of Beopjusa Gwaebultaeng with the royal family is also verified by a prayer at the bottom of the painting, reading "JusangJusamJeonhaSumanse (主上主三殿下壽萬歲, May the king live forever)." While looking into the historical characteristics of this art, this study took an approach based on scientific analysis. Damages to Beopjusa Gwaebultaeng include: bending, folding, wrinkles, stains due to moisture, pigment spalling, point-shaped pigment spalling, and pigment penetration to the lining paper at the back. According to the results of an analysis of the painting pigments, white lead was used as a white pigment, while an ink stick and indigo were used for black. For red, cinnabar and minium were used independently or were combined. For purple, organic pigments seem to have been used. For yellow, white lead and gamboge were mixed, or gamboge was painted over white lead, and gold foil was adopted for storage. As a green pigment, atacamite or a mixture of atacamite and malachite was used. Azurite and smalt were used separately or together as blue pigments.