• Proceedings of the PSK Conference
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• 2002.10a
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• pp.312.2-312.2
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• 2002

The metal-ligand complex, ［Ru(phen)$_2$(dppz)]^{2＋}$ (phen = 1.10-phenanthroline, dppz = dipyrido［3.2-a:2', 3'-c］phenazine) (RuPD), was used as a spectroscopic probe for studying nucleic acid dynamics. The RuPD complex displays a long lifetime and a molecular light switch property upon DNA binding due to shielding of its dppz ligand from water. (omitted)

• Proceedings of the KIEE Conference
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• 1996.07c
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• pp.1615-1617
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• 1996

In this study, epoxidized bisphenolic resins laminated with glass fiber mat(GFRP) are ivestigated on surface, bulk aspect and dielectric constant(${\varepsilon}'$ and ${\varepsilon}''$) vs. frequency characteristics with temperature. The investigation shows the different characteristics accordig to the attachments of fiber surface, filler content, matrix properties, and the others. Especially, dielectric properties of this sample are highly increased above $100^{\circ}C$ and decreased with the rise of frequency. There is a resonance at the high frequency region ($1MHz{\sim}10MHz$). So, dielectric properties show the shift with frequency and temperature. Dielectric properties of EGL 10 are higher than those of EGL 40 with the frequency. Generally, dielectric properties of EGL 10 are more unstable than those of EGL 40 on the shift of frequency and temperature.

• Proceedings of the KIEE Conference
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• 1996.07c
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• pp.1621-1623
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• 1996

The dielectric and polarizable properties of 0.85Pb$Mg_{1/3}Nb_{2/3}$)$O_3$-$0.125PbTiO_3$-$0.25BaTiO_3$ Ceramics have been investigated as a addition of the amount of $La_{2}O_{3}$($0{\leq}x{\geq}0.05$). The Temperature-dependant electrostictive characteristics of 0.85PMN-0.125PT-0.25BT relaxor ferroelectic system were improved by enhencing the extent of the diffuse phase transition(DPT). This was achieved using PMN-PT-BTceramics by the partial substitution of La at the Pb site. The curie temperature and the maximum dielectic permittivity decreased by substituting $La_{2}O_{3}$ and the electic field-related hysteresis phenomena decreased with increasing $La_{2}O_{3}$ substitution amount. It is showed decreasing in induced strain for electric field with increasing $La_{2}O_{3}$ substitution.

• Molecules and Cells
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• v.39 no.2
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• pp.103-110
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• 2016

As a degenerative joint disease, osteoarthritis (OA) constitutes a major cause of disability that seriously affects the quality of life of a large population of people worldwide. However, effective treatment that can successfully reverse OA progression is lacking until now. The present study aimed to determine whether two small non-coding RNAs miR-29a and miR-140, which are significantly down-regulated in OA, can be applied together as potential therapeutic targets for OA treatment. MiRNA synergy score was used to screen the miRNA pairs that potentially synergistically regulate OA. An in vitro model of OA was established by treating murine chondrocytes with IL-$1{\beta}$. Transfection of miR-29a and miR-140 via plasmids was investigated on chondrocyte proliferation and expression of nine genes such as ADAMTS4, ADAMTS5, ACAN, COL2A1, COL10A1, MMP1, MMP3, MMP13 and TIMP metallopeptidase inhibitor 1 (TIMP1). Western blotting was used to determine the protein expression level of MMP13 and TIMP1, and ELISA was used to detect the content of type II collagen. Combined use of miR-29a and miR-140 successfully reversed the destructive effect of IL-$1{\beta}$ on chondrocyte proliferation, and notably affected the MMP13 and TIMP1 gene expression that regulates extracellular matrix. Although co-transfection of miR-29a and miR-140 did not show a synergistic effect on MMP13 protein expression and type II collagen release, but both of them can significantly suppress the protein abundance of MMP13 and restore the type II collagen release in IL-$1{\beta}$ treated chondrocytes. Compared with single miRNA transfection, cotransfection of both miRNAs exceedingly abrogated the suppressed the protein production of TIMP1 caused by IL-$1{\beta}$, thereby suggesting potent synergistic action. These results provided1novel insights into the important function of miRNAs' collaboration in OA pathological development. The reduced MMP13, and enhanced TIMP1 protein production and type II collagen release also implies that miR-29a and miR-140 combination treatment may be a possible treatment for OA.

• The Journal of Engineering Research
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• v.5 no.1
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• pp.73-87
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• 2004

The synthesis and crystallization of amorphous calcium carbonate($CaCO_3$.$nH_2 O$) obtained from gas-liquid reaction between aqueous solution of calcium hydroxide and carbon dioxide at 15~$50^{\circ}C$ are investigated by electrical conductometry, XRD and TEM. The results are as follows: The initial reaction products prior to the formation of precipitated calcium carbonate is amorphous calcium carbonate. The electrical conductivity values in the slurry are decreased during the formation of amorphous calcium carbonate which covers particle surface of calcium hydroxide and retard the dissolution of calcium hydroxide into the solution. that amorphous calcium carbonate is unstable in the aqueous solution and crystallizes finally to calcite by the through-solution reaction. While amorphous calcium carbonate crystallizes into chain-like calcite, the conductivity values are recovered rapidly and the apparent viscosity of slurry containing higher concentration of calcium hydroxide increase. At below pH 9.5, chain-like calcite separates into individual particles to form precipitated calcium carbonate. The formation and synthetic temperature range of amorphous calcium carbonate is most suitable a primary decreasing step(a-step) at $15^{\circ}C$ in the electrical conductometry.

• Korean Journal of Microbiology
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• v.44 no.2
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• pp.93-97
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• 2008

RraA is a recently discovered protein inhibitor that regulates the enzymatic activity of RNase E, which plays a major role in the decay and processing of RNAs in Escherichia coli. It has also been shown to regulate the activity of RNase ES, a functional Streptomyces coelicolor ortholog of RNase E, which has 36% identity to the amino-terminal region of RNase E. There are two open reading frames in S. coelicolor genome that can potentially encode proteins having more than 35.4% similarity to the amino acid sequence of RraA. DNA fragment encoding one of these RraA orthologs, designated as RraAS2 here, was amplified and cloned in to E. coli vector to test whether it has ability to regulate RNase E activity in E. coli cells. Co-expression of RraAS2 partially rescued E. coli cells over-producing RNase E from growth arrest, although not as efficiently as RraA, induced by the increased ribonucleolytic activity in the cells. The copy number of ColEl-type plasmid in these cells was also decreased by 14% compared to that in cells over-producing RNase E only, indicating the ability of RraAS2 to inhibit RNase E action on RNA I. We observed that the expression level of RraAS2 was lower than that of RraA by 4.2 folds under the same culture condition, suggesting that because of inefficient expression of RraAS2 in E. coli cells, co-expression of RraAS2 was not efficiently able to inhibit RNase E activity to the level for proper processing and decay of all RNA species that is required to restore normal cellular growth to the cells over-producing RNase E.

• Journal of Korean Medicine Rehabilitation
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• v.30 no.4
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• pp.17-30
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• 2020

Objectives The purpose of this study is to evaluate the healing effect of Yukmijihwang-tang (YM) on femur fractured mice. Methods Mice were randomly divided into 6 groups: normal, control, positive control, YM with low, medium, high dosage each. All groups were prepared with femur fracture and treated diffrently. In order to measure bone regeneration effects, we analysed the levels of cyclooxygenase-2 (COX2), bone morphogenetic protein-2 (BMP2), collagen type II alpha 1 chain (Col2a1), Sox9, runt-related transcription factor 2 (Runx2), and osterix genes expressed in bone. For morphological analysis, muscles were removed and femur was observed with naked eye. Results COX2 gene expression in bone marrow significantly decreased. BMP2 gene expression significantly increased. Col2a1 gene expression significantly increased. Sox9 gene expression increased as well. Runx2 gene expression in bone marrow increased, but there was no statistical significance. Osterix gene expression significantly increased. Union of the fracture site progressed more in YM group compared to the control group. The fracture union score was significantly decreased in YM group compared to the control group. Conclusions YM showed anti-inflammatory effect, promoted bone regeneration by stimulating the bone regeneration factor. In conclusion, YM can help fracture healing and it well be applied clinically to patients with fracture.

• Korean journal of applied entomology
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• v.26 no.4 s.73
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• pp.195-201
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• 1987

Insects and nematodes associated with crops growing in plastic film houses were surveyed throughout the southern part of Korea at Jinju, Jiphyeon, Geumsan, Hapcheon, Changyeong, Namji, Milyang, Kimhae, Busan, Sooncheon and Gwangyang from December of 1984 to December of 1985. The phytonematodes representing six families, nine genera and nine species, and the insects representing four orders, seventeen families, twenty-three genera and twenty-four species were identified. The mite also caused problem on the leaves of strawberry and watermelon. Of these Meloidogyne incognita and Aphis gossyphii were most important ones. Aphelenchoides fragariae and M. hapla, however dominant nematodes on strawberry. M. incognita was always detected from pepper plants which were heavily infected with Phytophthora capsici. Tomato roots were readily infected with root-knot nematodes in non-sterilized upland surface soil. However, tomato were growing-vigorouly free from nematode damage in the upland surface soil treated by nematicide or in the paddy soil. A few galls were developed even in the upland subsoil at 60cm below surface. Soil salinity affected profoundly the host-root-knot nematode interaction: the numerous galls were developed on the tomato roots at EC $1mS/cm^2$ followed by 4mS and 2mS, but few galls were at 6mS.

• Natural Product Sciences
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• v.9 no.2
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• pp.109-111
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• 2003

A bioassay-guided fractionation of the active chloroform extracts of the rhizomes of Zingiber cassumunar Roxb. led to the isolation of a potential cytotoxic principle, curcumin (1), along with two inactive compounds, (E)-4-(3',4'- dimethoxyphenyl)but-3-en-1-ol (2) and (E)-4- (3',4'-dimethoxyphenyl)but-3-en-1- yl acetate (3). Curcumin (1) showed a significant cytotoxicity against several human cancer cell lines $(Col2;\;2.30,\;A549;\;12.30,\;SNU638;\;IC_{50}\;18.80\;{\mu}g/ml)$.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2005.05b
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• pp.106-109
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• 2005

The $(Sr_{1-x}Ca_x)TiO_3$(SCT) thin films are deposited on Pt-coated electrode (Pt/TiN/$SiO_2$/Si) using RF sputtering method with substitutional contents of Ca. The optimum conditions of RF power and Ar/$O_2$ ratio were 140[W] and 80/20, respectively. Deposition rate of SCT thin film was about 18.75[$\AA$/min]. The dielectric constant was increased with increasing the substitutional contents of Ca, while it was decreased if the substitutional contents of Ca exceeded over 15[mol%]. The capacitance characteristics had a stable value within ${\pm}4$[%] in temperature ranges of -80~+90[$^{\circ}C$].