• Title/Summary/Keyword: CGT

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Anti-inflammatory effect of CGT in atopic dermatitis model mice (아토피피부염을 유발한 마우스에서 청기해독탕의 항염증 효과)

  • Sueng, Yun-Chel
    • Journal of Digital Convergence
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    • v.12 no.8
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    • pp.361-368
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    • 2014
  • In order to investigate the effect of CGT on atopic dermatitis, various anti-inflammatory factors were studied. In-vitro, inflammatory mediators, such as MTT and nitric oxide and ROS were detected after the addition of LPS with or without CGT in RAW 264.7 cells. In-vivo, in order to verify the effectiveness of CGT in atopic dermatitis animal model, its role in inflammation factors and histological changes were observed in NC/Nga mice. CGT showed cell viability of 100% or higher in all concentration in RAW 264.7 cells. CGT inhibited LPS-induced productions of inflammatory mediators nitric oxide and antioxidant activity reactive oxygen species production in RAW 264.7 cells. CGT treated group showed significant decrease in serum of the expression of IL-$1{\beta}$, IL-6 and TNF-${\alpha}$ by 53%, 43% and 57% respectively. And CGT treated group showed decrease in serum of the expression of IgE by 56% respectively. Also, infiltration of adipocytes into skin was suppressed and the thickness of epidermis and dermis were relatively decreased in the CGT treated group. As a result, CGT has an anti-inflammatory effects in NC/Nga mouse. Thus, these results suggested a beneficial effect of CGT in treatment with Atopic dermatitis and inflammatory.

Analysis of Blood Components in Skin Color Reaction after Cupping Glass Therapy (부항시술 후 나타난 색소반응에 따른 혈액성분 분석)

  • Kweon Oh-Hyun;Bae Sung-Soo
    • The Journal of Korean Physical Therapy
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    • v.14 no.3
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    • pp.1-23
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    • 2002
  • Purpose: The purpose of this study was to examine the relationship between pigmental reaction and blood components, such as white blood cell (WBC), lymphocyte, monocyte, red blood cell (RBC), hemoglobin, mean corpuscular index (mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC)), hematocrit, platelet and neutrophil segment, after Cupping Glass Therapy (CGT). Subjects: Twenty-five healthy adults participated in this study; Methods: Subjects had undergone CGT for 5 days and were divided into two groups (dark color (DCG) and light color (LCG)) depending the level of pigmental reaction. Blood was collected from each subject at the beginning of this study and recollected after 5 day s'CGT. Results: The percentage of lymphocytes was higher in LCG than in DCG after CGT (p<.01) and was significantly increased in LCD after CGT (p<.01), The number of RBC was more in DCG than in LCG both before (p<.01) and after CGT (p<.05). The amount of hemoglobin was more in DCG than in LCG both before (p<.01) and after CGT (p<.01) and was significantly increased in LCD after CGT (p<.01). Mean corpuscular index showed that both MCH (p<.05) and MCHC (p<.01) were higher in DCG than in LCG after CGT, but only MCHC before CGT (p<.01). It also showed either decreased MCV (p<.01) or increased MCHC in LCD after CGT (p<.01). Hematocrite was higher in DCG than in LCG both before (p<.01) and after CGT (p<.01). The percentage of neutrophil segments was higher in BCG than In LCG after CGT (p<.01) and was significantly decreased in LCD after CGT (p<.05). However, neither the number of WBC and platelets nor the percentage of monocytes was significantly different between DCG and LCG either before or after CGT. Conclusion: Pigmental reaction was significantly related to the changes of blood conponents after CGT. The results of this study suggest that CGT may have an effect on the components of blood cells.

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Inhibitory Effect of Inflammatory Cytokines Secretion of Cheonggeumganghwa-tang in Mast cell (비만세포에서의 청금강화탕의 염증성 세포활성물질 분비 억제 효과)

  • Choi Young Soo;Moon Goo;Kim Dong Woung;Han Se Hee;Won Jin Hee
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.18 no.3
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    • pp.887-892
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    • 2004
  • Cheonggeumganghwa-tang(CGT) has been used for the purpose of prevention and treatment of bronchial asthma and allergic asthma in Korea. To investigate the biological effect of CGT, the author examined cytotoxicity and inflammatory cytokines secretion with human mast cell line, HMC-1. HMC-1 was stimulated with phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187. CGT by itself had no effect on viability of HMC-1. The effects of CGT on the secretion of tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6 from HMC-1 were evaluated with enzyme-linked immunosorbent assay (ELISA). CGT (1 ㎎/㎖) inhibited PMA plus A23187 -induced TNF-α and IL-6 secretion, by 93.86 ± 2.05%, 68.69 ± 2.86%, respectively. CGT also inhibited the NF-κB (p50) expression. Taken together, these results suggest that CGT inhibit the production of inflammatory cytokines in HMC-1 cells through blockade of NF-κB activation.

The Protective Effects of Chilgi-tang on Oxidative Stress by Glucose Deprivation in Neuro 2A Cells (Glucose deprivation으로 유발된 Neuro 2A 세포의 산화적 손상에 대한 칠기탕(七氣湯)의 보호효과)

  • Seong, Ki-Ho;Lee, Jung-Sup;Shin, Sun-Ho
    • The Journal of Korean Medicine
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    • v.31 no.2
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    • pp.1-18
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    • 2010
  • Objective: The water extract of Chilgi-tang (CGT) has been traditionally used in treatment of heart diseases caused by stress in Oriental Medicine. However, little is known about the mechanism by which CGT rescues neuronal cells from injury damage. Therefore, this study was designed to evaluate the protective effects of CGT on Neuro-2A cells by glucose deprivation-induced cell death. Methods: We investigated how cell death induced by glucose deprivation was associated with increased reactive oxygen species (ROS) generation. Result: The CGT treatment prior to glucose deprivation insult significantly reduced the number of cell deaths and the glucose deprivation-induced increase in ROS. Nitric oxide (NO) was also attenuated by CGT treatment. In addition, we demonstrated that the anti-cell death effect of CGT was blocked by heme oxygenase-1 (HO-1) activation. Finally, pretreatment of cells with a hemin, HO-1 inducer, reduced glucose deprivation-induced cell death. In contrast, pretreatment of cells with a ZnPP, HO-1 activity inhibitor, attenuated CGT-induced inhibition of cell death. Conclusions: These findings indicate that ROS plays an important role in glucose deprivation-induced cell death and that CGT may prevent glucose deprivation-induced cell death by inhibiting the ROS generation through HO-1 activation in Neuro-2A cells.

Slewing maneuver control of flexible space structure using adaptive CGT

  • Shimada, Yuzo
    • 제어로봇시스템학회:학술대회논문집
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    • 1995.10a
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    • pp.47-50
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    • 1995
  • This paper concerns an adaptive control scheme which is an extension of the simplified adaptive control. Originally, the SAC approach was developed based on the command generator tracker (CGT) theory for perfect model tracking. An attractive point of the SAC is that a control input can be synthesized without any prior knowledge about plant structure. However, a feedforward dynamic compensator of the CGT is removed from the basic structure of the SAC. This deletion of the compensator makes perfect model tracking difficult against even a step input. In this paper, an adaptive control system is redesigned to achieve perfect model tracking for as long as possible by reviving the dynamic compensator of the CGT. The proposed method is applied to slewing control of a flexible space structure and compared to the SAC responses.

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The Effect of Coptidis Rhizoma and Glycyrrhiza Uralensis on Lipid Deposition with Nonalcoholic Fatty Liver Disease (NAFLD) (황련-감초 추출물의 지방축적 감소를 통한 비알콜성지방간 개선 효과)

  • Ahn, Sang Hyun;Kim, Ki Bong
    • The Journal of Pediatrics of Korean Medicine
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    • v.33 no.2
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    • pp.12-21
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    • 2019
  • Objectives Coptidis Rhizoma and Glycyrrhiza Uralensis are herbs that treat obesity and dampness-phlegm. The aim of this study is to investigate the efficacy of Coptidis Rhizoma and Glycyrrhiza Uralensis on lipid deposition with nonalcoholic fatty liver disease (NAFLD). Methods Male 6-week-old C57BL/6 male mice were divided into three groups: control group (Ctrl), high fat diet group (HFF), and high fat diet with Coptidis Rhizoma and Glycyrrhiza Uralensis extract administration group (CGT). Each 10 mice were allocated to each group (total of 30 mice). All mice were allowed to eat fat rich diet freely throughout the experiment. To examine the effect of Coptidis Rhizoma and Glycyrrhiza Uralensis, we observed weight changes, total cholesterol and glucose levels, lipid blot distributions, PGC-1, p-$I{\kappa}B$, and p-JNK. Results Body weights for all mice were measured and analyzed the difference between the groups. Weight gain was significantly lower in CGT group than the HFF group. Total cholesterol and glucose levels were significantly lower in CGT group. The distribution of lipid blots and positive reaction of PGC-1 were significantly lower in CGT group. The positive reaction of p-$I{\kappa}B$ in hepatic tissues was significantly lower in CGT group. The positive reaction of p-JNK in hepatic tissues was significantly lower in CGT group. Conclusions Coptidis Rhizoma and Glycyrrhiza Uralensis have the effect of improving non - alcoholic fatty liver induced insulin resistance through regulation of lipid metabolism.

Expression of the Bacillus stearothermophilus NO2 CGTase gene in Saccharomyces cerevisiae (Saccharomyces cerevisiae 내에서 Bacillus stearothermophilus NO2 CGTnse 유전자의 발현)

  • 유동주;박현이;전숭종;권현주;남수완;김병우
    • Microbiology and Biotechnology Letters
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    • v.30 no.3
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    • pp.206-209
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    • 2002
  • For the expression of CGTase gene(cgtS) kom Bacillus stearothemophilus NO2 in Saccharomyces cerevisiae, cgtS gene was subcloned into the Eschepichia coll-yeast shuttle vector, pVT103-U. The constructed plasmid, pVT-CGTS was introduced to 5. cemi-siae 2805 cell, and then the cgtS gene under the control of adhl promoter was successfully expressed in the yeast transformant and 87% of the total activity was detected into the fermentation medium. Therefore, the signal peptide of B. stearothemephilus NO2 CeTase showed high secretion efficiency in 5. cerevisiae. Optimal conditions of the recombinant yeast cell f3r expression of CGTase was achieved, when 5. cerevisiae 2805/pv7-CGTS was cultivated on YP medium at 2% dextrose, pH 5.5,$30^{\circ}C$ and the expression level of CGTase was 0.624units/mL for 48 h culture.

Comparative Performance of Hens Fed Diets Containing Korean, Japanese and Chinese Green Tea

  • Uuganbayar, D.;Shin, I.S.;Yang, C.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.8
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    • pp.1190-1196
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    • 2006
  • This experiment was carried out to investigate the effects of Korean, Japanese and Chinese green tea on laying performance and egg quality in hens. A total of 168 "Tetran Brown" hens aged 40 weeks were assigned to 7 treatments in a completely randomized design. Each treatment had 4 replicates accommodating 6 layers per replication. The seven dietary treatments were: 1) control diet with no green tea added, 2) diet containing 1.0% Korean green tea (1.0% KGT), 3) diet containing 2.0% Korean green tea (2.0% KGT), 4) diet containing 1.0% Japanese green tea (1.0% JGT), 5) diet containing 2.0% Japanese green tea (2.0% JGT), 6) diet containing 1.0% Chinese green tea (1.0% CGT), and 7) diet containing 2.0% Chinese green tea (2.0% CGT). Egg production rate of the layers fed diets containing 1.0 or 2.0% green tea powders were significantly increased compared to that of the control (p<0.05). The egg weight of layers was significantly reduced in layers fed 1.0% CGT (p<0.05). The feed intake was significantly decreased in KGT and CGT groups at 2.0% inclusion levels (p<0.05). The egg shell thickness and shape index of JGT treatment was significantly lower than that of the control (p<0.05). There were no significant differences in albumen index, yolk index and Haugh unit of eggs for layers fed diets containing green tea powders regardless of origin (p>0.05). Green tea feeding to layers tended to reduce the overall cholesterol content of egg yolk. Particularly, 1.0 or 2.0% CGT significantly depressed the total cholesterol content of egg yolk (p<0.05). In conclusion, incorporation of 1.0 or 2.0% Korean, Japanese and Chinese green tea into layer diets regardless of origin had favorable effects on laying performance and egg quality profiles. Among the three green tea sources, the Chinese green tea powder had the highest reducing effect on cholesterol content in egg yolk.

An Analysis of Multidimensional Productivity for the Shipbuilding Performance (조선 성과 측정을 위한 다차원 생산성의 분석)

  • Kim, Yearnmin
    • Korean Management Science Review
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    • v.34 no.2
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    • pp.57-66
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    • 2017
  • The purpose of this study is to analyze the multidimensional productivity of the shipbuilding performance and to explain the role of different factors, such as man-hour, dock period, number of building block, launching process rate, automatic welding percent, and drawing fault rate which are important production-related variables in most shipbuilding companies. The shipbuilding productivity is obtained using Data Envelopment Analysis (DEA) approach. Then, a Tobit model is considered to measure the influence of different factors on the measured productivity. The results reveal that this productivity measure can substitute a representative shipbuilding productivity index (CGT/man-hour) in shipbuilding industries. Also, this multidimensional productivity analysis using DEA and Tobit reveals complex relationships between production-related variables and CGT and sale.

Decoupling Controller Design for the JINAMCHA by Using Command Generator Tracker and Eigenstructure Assignment (CGT와 고유구조 지정법을 이용한 지남차의 불연성화 제어기 설계)

  • Kim, Joo-Ho;Choi, Jae-Weon
    • Journal of the Korean Society for Precision Engineering
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    • v.16 no.6
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    • pp.176-182
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    • 1999
  • In this paper, we consider a decoupling control and a servo control for the JINAMCHA of which the cart and the pointer is coupled very strongly. The right eigenstructure assignment(i.e. simultaneous assignment of eigenvalues and right eigenvectors) is used for decoupling the motions of the cart and the pointer. The CGT(Command Generator Tracker) is used for a servo control of the JINAMCHA. The performance of the proposed control scheme, that is the combination of the right eigenstructure assignment and the CGT, is evaluated by simulations. The results show that the proposed controller has better performance than the LQR with limiting properties and the conventional LQ servo.

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