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http://dx.doi.org/10.14400/JDC.2014.12.8.361

Anti-inflammatory effect of CGT in atopic dermatitis model mice  

Sueng, Yun-Chel (Dept. of Oriental health care, Joongbu University)
Publication Information
Journal of Digital Convergence / v.12, no.8, 2014 , pp. 361-368 More about this Journal
Abstract
In order to investigate the effect of CGT on atopic dermatitis, various anti-inflammatory factors were studied. In-vitro, inflammatory mediators, such as MTT and nitric oxide and ROS were detected after the addition of LPS with or without CGT in RAW 264.7 cells. In-vivo, in order to verify the effectiveness of CGT in atopic dermatitis animal model, its role in inflammation factors and histological changes were observed in NC/Nga mice. CGT showed cell viability of 100% or higher in all concentration in RAW 264.7 cells. CGT inhibited LPS-induced productions of inflammatory mediators nitric oxide and antioxidant activity reactive oxygen species production in RAW 264.7 cells. CGT treated group showed significant decrease in serum of the expression of IL-$1{\beta}$, IL-6 and TNF-${\alpha}$ by 53%, 43% and 57% respectively. And CGT treated group showed decrease in serum of the expression of IgE by 56% respectively. Also, infiltration of adipocytes into skin was suppressed and the thickness of epidermis and dermis were relatively decreased in the CGT treated group. As a result, CGT has an anti-inflammatory effects in NC/Nga mouse. Thus, these results suggested a beneficial effect of CGT in treatment with Atopic dermatitis and inflammatory.
Keywords
Anti-inflammation; Atopic dermatitis; Cytokine; CGT; IgE; NC/Nga mouse;
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1 D. H. Kim, S. J. Park, J. Y. Jung, S. C. Kim, S. H. Byun, Antiinflammatory effects of the aqueous extract of Hwangnyenhaedok-tang in LPS-activated macrophage cells. Korean J. Oriental Physiology & Patholog. Vol. 24, No. 4, pp. 39-47, 2009.
2 S. Briganti, M. Picardo, Antioxidant activity, lipid peroxidation and skin diseases: What' new, Journal of the European Academy of Dermatology and Venereology, Vol. 17, No. 6, pp. 663-669, 2003.   DOI   ScienceOn
3 C. S. Koblenzer, Itching and the Atopic Skin, Journal of allergy and clinical immunology, Vol. 104, No. 3. pp. 109-13, 1999.
4 K. Mihara, K. Kuratani, T. Matusui, K.Yokota, Vital Role of the Itch-scratch Response in Development of Spontaneous Dermatitis in NC/Nga Mice, British Journal of Dermatology, Vol. 151, No. 2, pp. 335-45, 2004.   DOI   ScienceOn
5 N. Margarete, W. Thomas, Innate immunity allergy and atopic dermatitis, Current Opinion in Allergy and Clinical Immunology, Vol. 10, No. 5, pp. 463-468, 2010.   DOI   ScienceOn
6 J. H. Hong, A research Western medical science, The Korean traditional medicine, Local clinic and Naturopathy about Atopy, The Korean Society For Eco Early Childhood Education, Vol. 7, No. 1, 65-81, 2008.
7 S. H. Bang, Y. W. Choi, J. H. Ham, G. G. Hwan, The Clinical Effect of Topical DPCP Therapy in the Treatment of Atopic Dermatitis in Children and Adolescents, Korean Journal of Dermatology, Vol. 42, No. 1, pp. 29-36, 2004.   과학기술학회마을
8 S. Y. Jung, H. S. Seo, The Effects of Samhwangseje on Anti-Inflammation in RAW 264.7 cell. The Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology, Vol. 22, No.1, pp. 108-19, 2009.   과학기술학회마을
9 E. M. Behrens, Macrophage activation syndrome in rheumatic disease: What is the role of the antigen presenting cell, Autoimmunity reviews, Vol. 7, No. 4, pp. 305-8, 2008.   DOI   ScienceOn
10 L. N. Lopez-Bojorquez, A. Z. Dehesa, G. Reyes-Teran, Molecular mechanisms involved in the pathogenesis of septic shock, Archives of medical research, Vol. 35, No. 6, pp. 465-79, 2004.   DOI   ScienceOn
11 K. Toshiaki, S. J. Galli, Regulation of mast-cell and basophil function and survival by IgE, Nature Reviews Immunology, Vol. 2, pp. 773-86, 2002.   DOI   ScienceOn
12 M. J. Tak, M. R. Tak, K. H. Kang, W. S. Ko, H. J. Yoon, The Inhibitory Effects of Yang Geouk San Hwa-Tang on LPS-stimulated inflammation in RAW264.7 macrophage cells, The Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology, Vol. 23, No.1, pp. 118-34, 2010.   과학기술학회마을
13 J. H. Lee, Antioxidant activities of Eclipta alba L. ethantol-extract, Annals of Plant Resources Research, Vol. 7, No. -, pp. 75-85, 2008.
14 I. H. Ryu, H. B. Cho, S. B. Kim, Y. J. Seo, C. M. Choi, The inhibitory effect of Picrasmae Lignum on inflammatory responses, The Journal of Oriental Obstetrics & Gynecology, Vol. 24, No. 1, pp. 1-14, 2011.   과학기술학회마을
15 C. S. Kim, J. H. Yoon, D. H. Park, The Effects of Exercise at Anaerobic Threshold Intensity on Production of NO, IL-1 beta and IFN-gamma in Young Males, Journal of the Korean Society of Living Environmental System, Vol. 19, No. 3, pp. 381-391, 2012.
16 G. S. Lee, I. D. Pana, J. Y. Choi, S. Y. Yoon, J. H. Choi, T. J. Kang, S. K. Oh, J. H. Cheong, Effect of SPZZC, a composotion of herb extracts, on atopic dermatitis in BALB/c and NC/Nga mouse, Yakhak Hoeji. Vol. 52, No. 3, pp. 232-239, 2008.
17 M. L. McDaniel, G. Kwon, J. R. Hill, C. A. Marshall, J. A. Corbett, Cytokines and nitric oxide in islet inflammation and diabetes, Experimental Biology and Medicine, Vol. 211, No. 1, pp. 24-32, 1996.   DOI