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Expression of the Bacillus stearothermophilus NO2 CGTase gene in Saccharomyces cerevisiae  

유동주 (동의대학교 미생물학과)
박현이 (동의대학교 미생물학과)
전숭종 (일본산업기술종합연구소)
권현주 (미쯔비시 화학생명과학연구소)
남수완 (동의대학교 미생물학과)
김병우 (동의대학교 미생물학과)
Publication Information
Microbiology and Biotechnology Letters / v.30, no.3, 2002 , pp. 206-209 More about this Journal
Abstract
For the expression of CGTase gene(cgtS) kom Bacillus stearothemophilus NO2 in Saccharomyces cerevisiae, cgtS gene was subcloned into the Eschepichia coll-yeast shuttle vector, pVT103-U. The constructed plasmid, pVT-CGTS was introduced to 5. cemi-siae 2805 cell, and then the cgtS gene under the control of adhl promoter was successfully expressed in the yeast transformant and 87% of the total activity was detected into the fermentation medium. Therefore, the signal peptide of B. stearothemephilus NO2 CeTase showed high secretion efficiency in 5. cerevisiae. Optimal conditions of the recombinant yeast cell f3r expression of CGTase was achieved, when 5. cerevisiae 2805/pv7-CGTS was cultivated on YP medium at 2% dextrose, pH 5.5,$30^{\circ}C$ and the expression level of CGTase was 0.624units/mL for 48 h culture.
Keywords
Bacillus stearothermophilus; cyclodextrin glucanotransferase; E. coli-yeast shuttle vector; Saccharomyces cerevisiae;
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